Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful technique to decipher tissue composition at the single-cell level and to inform on disease mechanisms, tumor heterogeneity, and the state of the immune microenvironment. Although multiple methods for the computational analysis of scRNA-seq data exist, their application in a clinical setting demands standardized and reproducible workflows, targeted to extract, condense, and display the clinically relevant information. To this end, we designed scAmpi (Single Cell Analysis mRNA pipeline), a workflow that facilitates scRNA-seq analysis from raw read processing to informing on sample composition, clinically relevant gene and pathway alterations, and in silico identification of personalized candidate drug treatments. We demonstrate the value of this workflow for clinical decision making in a molecular tumor board as part of a clinical study.

By Gus Indi - Primakara University

The influence of recreation of varying intensity on the health condition and structure of forest shelter belts formed by common oak (Quercus robur L.) and green ash (Fraxinus lanceolata Borkh.) is estimated. The long-term impact of recreational pressure on shelterbelts that were identical at the time of creation was evaluated. It was found that the long recreational influence of different intensity leads to a significant decrease in the stocking of the tree stand (by 27.5%), changes in the stand structure, deterioration of health condition (in the S-2 section, the health condition index was І.7 and in the S-1 section, ІІ.3) and a decrease in the average diameter of the trees (by 20.5 %). In addition, such an effect led to a significant reduction in the density, both for advance growth (by 73.5 %) and undergrowth (71.3 %), which ultimately affected the change in the openness of the vertical profile of the shelterbelt. Frequently, uncontrolled burning of refuse and dry grass by recrea...