- DALAT UNIVERSITY JOURNAL OF SCIENCE Volume 10, Issue ANTIBACTERIAL ACTIVITIES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL OF BLUMEA BALSAMIFERA (L.) DC., DISTRIBUTED IN LAMDONG PROVINCE, VIETNAM Hoang Thi Binha*, Nguyen Minh Tria, Nguyen Huu Quana, Nguyen Van Ngoca a The Faculty of Biology, Dalat University, Lamdong, Vietnam * Corresponding author: Email:
[email protected] Article history Received: August 25th, 2019 Received in revised form (1st): October 28th, 2019 | Received in revised form (2nd): December 19th, 2019 Accepted: December 23rd, 2019 Abstract In the present study, the chemical composition and the antibacterial properties of the essential oil obtained from fresh leaves of Blumea balsamifera (L.) DC.
- The hydrodistillation method was used to isolate essential oil from leaves of this species, and gas chromatography/mass spectrometry (GC-MS) techniques were used to analyze the chemical constituents of the essential oil.
- Thirty six chemical constituents of the essential oil derived from fresh leaves of B.
- balsamifera were identified, in which the major compounds of the essential oil were camphor, caryophyllene, caryophyllene oxide, β-eudesmol, thymol hydroquinone dimethyl ether, and t-eudesmol, accounting for and 3.32%, respectively.
- Blumea balsamifera.
- Essential oil.
- Licensing: This article is licensed under a CC BY-NC 4.0 3 DALAT UNIVERSITY JOURNAL OF SCIENCE [NATURAL SCIENCES AND TECHNOLOGY] THÀNH PHẦN HOÁ HỌC VÀ HOẠT TÍNH KHÁNG KHUẨN CỦA TINH DẦU BLUMEA BALSAMIFERA (L.) DC.
- PHÂN BỐ Ở LÂM ĐỒNG, VIỆT NAM Hoàng Thị Bìnha*, Nguyễn Minh Tría, Nguyễn Hữu Quâna, Nguyễn Văn Ngọca Khoa Sinh học, Trường Đại học Đà Lạt, Lâm Đồng, Việt Nam a * Tác giả liên hệ: Email:
[email protected] Lịch sử bài báo Nhận ngày 25 tháng 8 năm 2019 Chỉnh sửa lần 01 ngày 28 tháng 10 năm 2019 | Chỉnh sửa lần 02 ngày 19 tháng 12 năm 2019 Chấp nhận đăng ngày 23 tháng 12 năm 2019 Tóm tắt Trong nghiên cứu này, thành phần hoá học và hoạt tính kháng khuẩn của tinh dầu thu từ lá tươi loài Blumea balsamifera (L.) DC.
- balsamifera (L.) DC.
- Từ khóa: Blumea balsamifera.
- Almost all the species of the Blumea genus are widely distributed in tropical Asia with a few species in Australia and Africa (Anderberg, 1994, pp.
- In particular, Blumea balsamifera is widely distributed in many areas of Vietnam.
- Chantrapromma, 2011) and components of essential oil from leaves of B.
- Chu et al., 2012.
- In Bangladesh, the chemical compositions of the essential oil from leaves of B.
- and γ-eudesmol (3.18%) (Bhuiyan et al., 2009).
- In China, the main components of the essential oil of B.
- and caryophyllene oxide (5.35%) (Chu et al., 2012).
- In Vietnam, chemical compositions of the essential oil from the leaves of B.
- and thujopsene were the dominant components in the essential oil.
- Chemical compositions of the essential oil of B.
- Chu et al.
- 5 DALAT UNIVERSITY JOURNAL OF SCIENCE [NATURAL SCIENCES AND TECHNOLOGY] Nguyen et al.
- and Tran et al.
- However, the essential oil compositions may be affected by the choice of extraction methods.
- Moreover, until now there has been no report on the volatile constituents of essential oil composition as well as antibacterial activity of essential oil derived from the leaves of B.
- Thus, in this study, we report the chemical composition and the evaluation of antibacterial activity of the essential oil from the leaves of B.
- Plant materials Fresh leaves of Blumea balsamifera L.
- Isolation of the essential oil In the present study, the hydrodistillation method was used to extract essential oil from B.
- After the volatile essential oil was collected, sodium sulphate was used to dry the anhydrous essential oil of B.
- Gaschromatography-mass spectrometry (GC-MS) and identification of the constituents The components of the essential oil derived from leaves of B.
- Most of the constituents of the essential oil were identified on the basis of retention times (RT).
- The antibacterial activity of the essential oil derived from fresh leaves of B.
- In the center of each dish, wells of approximately 6 mm diameter were created and 40 μL of essential oil solution, dimethyl sulphoxide (DMSO), and chloramphenicol were added to the wells.
- The sterile DMSO was used to the dilute essential oil of B.
- Sensitive: D > 13 mm) showed that both tested bacteria strains were not sensitive (D > 13 mm) to the essential oil of B.
- Coli) of bacteria were moderately sensitive to the essential oil.
- The activity against bacteria of the essential oil of B.
- the main component of the essential oil.
- Camphor is mainly responsible for the antibacterial activity of the plant oils which contain it, as it is known to have very efficient antibacterial properties (Jalsenjak, Peljnjak.
- Sivropoulou et al., 1997).
- CONCLUSION In conclusion, analysis by GC-MS showed that thirty six compounds were identified in the essential oil obtained from fresh leaves of Blumea balsamifera in Lamdong, Vietnam.
- In addition, the essential oil of this species showed significant antibacterial activity against both E.
- We thank the staff of the Center of Analytical Services and Experimentation Hochiminh City for analysis of our essential oil samples by the GC-MS method.
- Identification of essential oil components by gas chromatography/quadrupole mass spectrometry (4th ed.
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