- Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. - In total, 972 circRNAs were identified, including 631 stage-specific circRNAs and 8 tissue-specific circRNAs. - The biological functions of parental genes of circRNAs were enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. - Furthermore, the head-to-tail exon as well as the expressions of 10 circRNAs were validated by the divergent RT-qPCR and sanger sequencing.. - Conclusions: In summary, the profiles of ovarian circRNAs were provided during pubertal transition in gilts, and these results provided useful information for the investigation on the onset of puberty at the ovarian-circRNAs-level in mammals.. - The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. - If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. - These observa- tions suggested the potential importance and signifi- cance of circRNAs in the ovary, but the information of ovarian circRNAs remains little during the pubertal transition.. - Then, the expression changes of circRNAs were ex- plored as well as the stage-specific and tissue-specific circRNAs, and the potentially pubertal circRNAs were detected in this study. - A total of 972 cir- cRNAs candidates were identified in the pubertal transi- tion of pubertal ovaries (Additional file 1) (Fig. - Thereinto, 347, 293 and 827 circRNAs were identified in pre-, in- and post-puberty, respectively (Fig. - These circRNAs were more distributed in Sus scrofa chromo- some 1 (Fig. - The expressions of circRNAs were the lowest in the post-puberty (Fig. - Notably, in the pre- puberty, exonic, intronic and intergenic circRNA occu- pied 93.66. - in the in- puberty, exonic, intronic and intergenic circRNA occu- pied 94.20. - in the post- puberty, exonic, intronic and intergenic circRNA occu- pied 95.41. - Additionally, most circRNAs were made up of two and three exons. - Specifically, circRNAs were made up of two exons occupied 26.80. - circRNAs were made up of three exons occupied 34.87. - 922 exonic circRNAs were derived from 699 genes, and 23 intronic circRNAs were derived from 23 genes (Fig. - Taken together, 972 circRNAs were identified during onset of puberty in the ovaries of gilts.. - In the steroid biosynthesis sig- naling pathway, “circ . - and de- rived from FDFT1 gene were uniquely expressed in the post-puberty (Fig. - In the autophagy-animal signaling pathway, “circ derived from HIF1A gene was uniquely expressed in the in-puberty.. - In the MAPK signaling pathway, “circ . - 25143159” and “circ derived from MAP3K2 gene was uniquely expressed in the post- puberty. - In the progesterone-mediated oocyte matur- ation, “circ derived from CPEB4 gene was uniquely expressed in the post-puberty.. - In the ras signaling pathway, “circ . - 43691261” derived from NF1 gene was expressed in the in-puberty and post-puberty. - Details of these circRNAs were shown in Additional file 3. - uniquely expressed in the pre- puberty) and “circ expressed in the pre-, in- and post-puberty) were derived from ESR1;. - “circ uniquely expressed in the post-puberty) was derived from JAK2. - expressed in the in- and post- puberty) was derived from NF1. - Apart from “circ . - Strikingly, in the four types of AS events, IR showed more extreme post-pubertal tendency (Welch two- sample t-test, P <. - Furthermore, “circ . - a CircRNAs were identified by two algorithms. - c Circos plot of the circRNAs distribution in the whole genome of gilts. - f Transcript length of circRNAs. - Stage-specific and ovary-specific circRNAs in the pubertal transition. - Respect- ively, 72, 50 and 509 of circRNAs were uniquely expres- sion in pre-, in- and post-puberty stages and considered. - The parental genes of these stage-specific circRNAs were enriched in MAPK signaling pathway, progesterone-mediated oocyte maturation, oocyte meiosis and GnRH signaling pathway in post-puberty (Additional file 6) (Fig. - Moreover, 154 circRNAs were expressed in all stages and consid- ered as the co-existed circRNAs (Figs. - Be- sides, some specific circRNAs and co-existed circRNAs were derived from the same gene. - For instance, “circ 1:. - uniquely expressed in the post- puberty) and “circ no uniquely expressed in any puberty) were derived from SMAD4 (Additional file 7). - investigate the specific circRNAs in the ovaries, 964 known circRNAs that were found in nine tissues (brain, heart, kidney, liver, lung, skeletal muscle, spleen, testis, and retina) were excluded, leaving 8 circRNAs as being putative ovary-specific circRNAs which were only expressed in the ovary (Additional file 8). - Subsequently, we found that the length of ovary-specific circRNAs were shorter than known circRNAs (Fig. - Strikingly, apart from “circ other 7 putative ovary- specific circRNAs were exclusively expressed in the post- puberty (Fig. - Besides, “circ . - and “circ were both derived from NR5A2 (Additional file 8). - the stage-specific means during pubertal transition, and 8 circRNAs were the ovary-specific circRNAs.. - Subsequently, 154 co-existed circRNAs were used to analysis differential expression between pair-wise com- parison of three stages (Figs. - In total, 10 cir- cRNAs were identified as differentially expressed circRNAs (Additional file 9), of which 7 up-regulated circRNAs and 3 down-regulated circRNAs were identi- fied in the pre- vs. - 2 up-regulated circRNA were identified in the in- vs. - For instance, “circ . - which was down-regulated in the pre- vs. - Validation of circRNAs. - The head- to-tail splice junctions of 5 circRNAs were determined by sanger sequencing, which proved that the circRNAs were circRNAs (Fig. - Furthermore, 7 circRNAs of 10 differentially expressed circRNAs were selected for fur- ther investigation. - The “circ . - 6b), “circ Fig. - 6c), “circ 2:. - 6d), “circ . - 6e), “circ . - 6f), “circ Fig. - Besides, the expression of “circ . - Consistently, in this study, we demonstrated that exonic circRNAs were accounted for approximately 94. - Notably, for autophagy- animal pathway, “circ was ex- clusively expressed in the in-puberty, and its parental gene HIF1A has been reported to have the highest ex- pression in the early luteal phase [54]. - In this study, we found that the AS events in circRNAs were consistent with previous reports, of which A3SS and ES events occurred in circRNAs in abundance. - Importantly, the parental genes of post-pubertal specific circRNAs were associated with the function of growth, development and reproduction.. - the parental gene of “circ . - There is evidence that FAF1, Fas-associated protein factor 1, is expressed in the cytoplasm of grow- ing oocyte of the ovary [63]. - These results suggest that the tissue-specific circRNAs might regulate the transcriptions of their parental genes to involve in the onset of puberty.. - Besides, many of target genes regulated by circRNAs- miRNAs network are the important puberty related genes and differentially expressed in the pubertal ovaries (Fig. - For example, GAS2 is a regulator in the ovary during folliculogenesis and oocyte cyst breakdown [43];. - AIG1 is induced by androgen and expressed at high levels in the ovaries [44]. - post-puberty group, the down-regulated “circ 9:. - In conclusion, we described the profiles of ovarian 972 circRNAs during pubertal transition in gilts, and these circRNAs were mostly enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras sig- naling pathway. - 631 circRNAs were stage-specific, 8. - circRNAs were tissue-specific, and 10 circRNAs were differentially expressed across pre-, in- and post-pubertal ovarian. - Besides, 5 circRNAs were derived from four pu- bertal genes ESR1, JAK2, NF1 and ARNT. - Furthermore, several circRNAs were validated by the di- vergent RT-qPCR. - We eventually identified candi- date circRNA in the gilts during pre-, in- and post- puberty. - In addition, the selection criteria for tissular specificity was as follows: the circRNAs identified in this study were matched with the known circRNAs in pigs by start- ing and ending the genome locations of circRNAs, and the new circRNAs were considered as the presumed tis- sue specific circRNAs. - The known circRNAs were down- loaded from circAtlas 2.0 (namely, the circRNAs database in vertebrates) which were included circRNAs of nine tissues (brain, retina, heart, kidney, liver, lung, skeletal muscle, spleen, and testis) [73]. - In addition, the alternative splicing events of circRNAs were determined by the CIRI-AS module [40], which classified the alter- native splicing events into four forms: A3SS, A5SS, ES, and IR. - The divergent primers of 10 circRNAs were designed to verify the accuracy of the RNA-sEq. - Frequency and amplitude of gonadotropin-releasing hormone stimulation and gonadotropin secretion in the rhesus monkey. - In vivo release of luteinizing hormone releasing hormone increases with puberty in the female rhesus monkey.. - Intrafollicular level of steroid hormones and the expression of androgen receptor in the equine ovary at puberty. - Ro S, Song R, Park C, Zheng H, Sanders KM, Yan W: Cloning and expression profiling of small RNAs expressed in the mouse ovary. - Li Z, Huang C, Bao C, Chen L, Lin M, Wang X, Zhong G, Yu B, Hu W, Dai L et al : Exon-intron circular RNAs regulate transcription in the nucleus. - Xia S, Feng J, Lei L, Hu J, Xia L, Wang J, Xiang Y, Liu L, Zhong S, Han L et al : Comprehensive characterization of tissue-specific circular RNAs in the human and mouse genomes. - Xie S, Li M, Chen Y, Liu Y, Ma L, Sun X, Sun Y, Gao R, Huang T: Identification of circular RNAs in the ovarian follicles of Meishan and Duroc sows during the follicular phase. - Cao Z, Gao D, Xu T, Zhang L, Tong X, Zhang D, Wang Y, Ning W, Qi X, Ma Y et al : Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation. - Zhao C, Zhou Y, Shen X, Gong M, Lu Y, Fang C, Chen J, Ju R: Circular RNA expression profiling in the fetal side of placenta from maternal polycystic ovary syndrome and circ_0023942 inhibits the proliferation of human ovarian granulosa cell. - Hiney JK, Srivastava VK, Vaden AD, Hartzoge NL, Dees WL: >Regulation of Kisspeptin Synthesis and Release in the Preoptic/Anterior Hypothalamic Region of Prepubertal Female Rats: Actions of IGF-1 and Alcohol. - Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW: Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary. - Russell MC, Cowan RG, Harman RM, Walker AL, Quirk SM: The hedgehog signaling pathway in the mouse ovary
Xem thử không khả dụng, vui lòng xem tại trang nguồn hoặc xem
Tóm tắt