- A total of 96.79% of the raw read sequences were processed as clean reads. - Of a total of 50,230 unigenes of the total unigenes) annotated to homologous proteins in the Protostome database (PANM-DB). - Full list of author information is available at the end of the article. - Most of the slug species were able to expand their habitat requirements over in mountains. - Hence, cataloguing the genomic resources would guide successful establishment of the species in the wild.. - In the case of molluscan species, these microsatellites would be valuable in genetic improvement of stocks through marker-assisted breeding and understanding the diversity of the species within and among populations [16, 17]. - This is to in- vestigate the ecological dynamics of the species through screening of growth, immunity, and reproduction related genes. - BUSCO version using the metazoan_odb9 [25] was used to create a lineage dataset and assess the completeness of the transcriptome.. - De novo assembly of the clean sequence reads was conducted using the Trinity assembly, generating 591,401 contigs with the lar- gest contig size of 15,717 bp. - Based on BUSCO, we assessed the “completeness” of the I.. - Most of the unigenes (34,470) showed homologous matches to PANM-DB. - 9457 sequences (27.44%) out of the 34,470 show specific annotation to the homologous proteins in PANM-DB. - Of the 50,230 unigenes, 18,238 showed classic and conserved InterPro domains. - Rest of the sequences par- ticipated under more than one GO term. - The GO terms (at level 2) for each of the three categories are shown in Fig. - In the. - An exhaustive summary of the candidate genes under immune defense categories such as ‘pathogen rec- ognition receptor (PRR. - Furthermore, genes involved in the unique features of the slug’s. - In silico analysis of the protein sequence revealed the characteristic overlapping of the PGRP and N-acetylmuramoyl-L-alanine amidase domains (amidase_2 domain). - We also found profilin-like and profilin-4-like transcripts involved in the restructuring of the actin cytoskeleton. - Angiotensin-converting enzyme-like If_Uni_08052, If_Uni_10667, If_Uni Type-1 angiotensin II receptor-associated. - If_Uni_23153 1732. - Type-1 angiotensin II receptor B-like If_Uni_28079, If_Uni . - Adenylate cyclase isoform X1 If_Uni_27752, If_Uni . - Adenylate cyclase type 2-like If_Uni_27754, If_Uni Adenylate cyclase If_Uni_30138, If_Uni_32170, If_Uni_32295,. - If_Uni_35390, If_Uni_36571. - 5 ′ -AMP-activated protein kinase If_Uni_06408, If_Uni_10162, If_Uni_16111, If_Uni_17195, If_Uni_31806, If_Uni_45007, If_Uni_45226, If_Uni_49689. - Aquaporin, partial If_Uni_13477, If_Uni_36565, If_Uni Aquaporin-4-like If_Uni_27284, If_Uni_27285, If_Uni_40352,. - If_Uni_48767. - Aquaporin AQPcic-like If_Uni_30154, If_Uni . - Aquaporin-8-like If_Uni_30734 1971. - Aquaporin AQPAn.G-like If_Uni_37263, If_Uni . - Aquaporin-9-like If_Uni_39587 1799. - Collagen alpha-1(I) chain-like If_Uni_08744, If_Uni_08745, If_Uni_09485, If_Uni_09486, If_Uni_15928, If_Uni_27766, If_Uni_27767, If_Uni_29582, If_Uni_34918, If_Uni_37761, If_Uni_39405, If_Uni_39724. - NMDA-type glutamate receptor If_Uni_38285 1269. - Glutamate receptor ionotropic, NMDA 2B-like If_Uni_41604 278. - Heat shock protein 70 B2-like If_Uni_09052, If_Uni_09053, If_Uni_23324, If_Uni_23325, If_Uni_23326. - Heat shock 70 kDa protein cognate 4 If_Uni_23327, If_Uni_23328, If_Uni_33435, If_Uni_43426. - Heat shock 70 kDa protein 4 If_Uni_42286 4176. - Heat shock 70 kDa protein 10 If_Uni_43360 285. - Heat shock 70 kDa protein 13 If_Uni_36140, If_Uni . - Heat shock 70 kDa protein 14 If_Uni_22068, If_Uni_22069, If_Uni . - Hsp70-binding protein 1 If_Uni_41490 2947. - Insulin receptor substrate 1 If_Uni_44383 1014. - Mitogen-activated protein kinase 15 If_Uni_28827, If_Uni calcium-independent phospholipase. - If_Uni_28664, If_Uni . - group XV phospholipase A2-like If_Uni_31585 1836. - If_Uni_32679, If_Uni_38324, If_Uni . - stimulated by retinoic acid gene 6 protein If_Uni_26478, If_Uni_26479, If_Uni_26480, If_Uni_26481, If_Uni_27267, If_Uni_27367, If_Uni_27368, If_Uni_27369, If_Uni_33908, If_Uni_46952. - T-box transcription factor TBX20-like If_Uni_21931, If_Uni T-box transcription factor TBX1-B-like If_Uni_25334, If_Uni_25335, If_Uni_03651,. - If_Uni_03826. - Solute carrier family 41 If_Uni_07293, If_Uni_24931, If_Uni_24932, If_Uni_24933, If_Uni_24934, If_Uni_24935, If_Uni_24936, If_Uni_35601. - The basic idea of this study was to ex- plore the fitness traits regulating adaptation and survival of the species in the wild. - Solute carrier family 25 If_Uni_08851, If_Uni_08852, If_Uni_08853, If_Uni_08854, If_Uni_08855, If_Uni_10412, If_Uni_15275, If_Uni_15276, If_Uni_15277, If_Uni_15278, If_Uni_18168, If_Uni_30488, If_Uni_09808, If_Uni_33449, If_Uni_33450, If_Uni_34586, If_Uni_35960, If_Uni_38390, If_Uni_40358, If_Uni_41902, If_Uni_45240, If_Uni_47602, If_Uni_48696, If_Uni_00611. - Solute carrier family 40 If_Uni_09262, If_Uni_09263, If_Uni_22596, If_Uni_22597, If_Uni_30690. - Solute carrier family 43 If_Uni_09267, If_Uni_09268, If_Uni_37142, If_Uni_43186, If_Uni_06623. - Solute carrier family 22 If_Uni_10312, If_Uni_15224, If_Uni_19826, If_Uni_22045, If_Uni_24463, If_Uni_24464, If_Uni_24465, If_Uni_24466, If_Uni_24467, If_Uni_24468, If_Uni_24469, If_Uni_26545, If_Uni_28975, If_Uni_28976, If_Uni_32419, If_Uni_32420, If_Uni_34348, If_Uni_36091, If_Uni_36557, If_Uni_36908, If_Uni_37399, If_Uni_37943, If_Uni_43214, If_Uni_49180, If_Uni_50058, If_Uni_00679, If_Uni_04935. - Solute carrier family 23 If_Uni_12119, If_Uni_36506, If_Uni_37977, If_Uni_40499, If_Uni_47883. - Solute carrier family 35 If_Uni_12656, If_Uni_12657, If_Uni_14403, If_Uni_19966, If_Uni_27046, If_Uni_27993, If_Uni_29331, If_Uni_29332, If_Uni_32495, If_Uni_35319, If_Uni_39687, If_Uni_46340, If_Uni_49558, If_Uni_00045, If_Uni_06869. - Solute carrier family 12 If_Uni_15628, If_Uni_15629, If_Uni_15630, If_Uni_15631, If_Uni_17088, If_Uni_17089, If_Uni_24560, If_Uni_24561, If_Uni_24562, If_Uni_24563, If_Uni_24564, If_Uni_24565, If_Uni_24566, If_Uni_24902, If_Uni_24903, If_Uni_24904, If_Uni_28839, If_Uni_30662, If_Uni_39565, If_Uni_45407, If_Uni_48974. - Solute carrier family 28 If_Uni_19488, If_Uni_19489, If_Uni_19743, If_Uni_24733, If_Uni_24734, If_Uni_24735, If_Uni_26540, If_Uni_26541, If_Uni_26542. - Solute carrier family 15 If_Uni_27273, If_Uni_28577, If_Uni_30039, If_Uni_30040, If_Uni_31286, If_Uni_31287, If_Uni_32935, If_Uni_39335. - Solute carrier family 46 If_Uni_32274, If_Uni . - Solute carrier family 13 If_Uni_34257, If_Uni_43800, If_Uni . - Solute carrier family 45 If_Uni_36376 1546. - Again, KEGG is a unified database connecting gen- omic information to biological pathways and provides a foresight towards the putative enzymes in the genome of the species [45].. - With the transcriptome information of the pul- monate slug, I. - The CUE domain comprised of the conserved ‘Met-Phe-Pro’. - or phagocytosis of the cell [66, 67]. - The lack of a signal peptide in the I. - In this case, TNF (tumor necrosis factor) receptor-associated factor 6 (TRAF6)/TRAF3 is recruited in the cytosol upon activation of the TLR-MyD88 signaling cascade. - fruhstorferi for understanding the phyl- ogeny of the species under the molluscan clade [91].. - fruhstorferi health and sustainability in the wild.. - The cataloguing of immune-related functional transcripts from the de novo assembled transcriptome of the air-. - This transcript is one of the many associated with hermaphrodite phenotypes in Caenorhabditis elegans [96]. - In the transcriptome of C.. - Sox30, de- fined to be involved in the differentiation of male gametes was found to be highly expressed in the testis of the Manila clam R. - Spermatogenesis-associated protein 6 was found highly expressed in male tissues of the abalone H. - Most of the transcripts found are suggested to be associated with male reproductive tissues. - BLASTx annotation of the unigenes to PANM DB at an E-value threshold of 1.0E-5 was used for the statistical summary.. - Validation of the I. - (A) RT-PCR analysis of the whole-body sample using gene-specific primers. - A catalogue of the mollusks of Jeju Island, South Korea. - Cloning and sequencing of three C-type lectins from body surface mucus of the land slug, Incilaria fruhstorferi. - The whole -genome and transcriptome of the Manila clam (Ruditapes philippinarum). - Mitochondrial phylogenomics of the Bivalvia (Mollusca):. - Sequencing and de novo assembly of visceral mass transcriptome of the critically endangered land snail Satsuma myomphala: annotation and SSR discovery. - Sequencing, De novo assembly, and annotation of the transcriptome of the endangered freshwater pearl bivalve, Cristaria plicata, provides novel insights into functional genes and marker discovery. - Transcriptome analysis of the Sydney rock oyster, Saccostrea glomerata: insights into molluscan immunity. - Transcriptome sequencing and differential gene expression analysis of the schistosome-transmitting snail Oncomelania hupensis inhabiting hilly and marshland regions. - The transcriptome of a ‘ sleeping ’ invader: de novo assembly and annotation of the transcriptome of aestivating Cornu aspersum. - Transcriptome analysis of the threatened snail Ellobium chinense reveals candidate genes for adaptation and identifies SSRs for conservation genetics. - De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome. - RNA sequencing, De novo assembly, functional annotation and SSR analysis of the endangered diving beetle, Cybister chinensis. - Sequencing and de novo assembly of the Asian clam (Corbicula fluminea) transcriptome using the Illumina GAIIx method. - Transcriptome sequencing and de novo annotation of the critically endangered Adriatic sturgeon. - Transcriptomic analysis of the endangered Neritid species Clithon retropictus:. - Identification, characterization and expression profiling of the Tollip gene in yesso scallop (Patinopecten yessoensis). - Transcriptome analysis of the Portunus trituberculatus: De novo assembly, growth-related gene identification and marker discovery. - transcriptome of the gray garden slug
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