Methods for production of chitin and chitosan

- FIELD OF THE INVENTION.
- BACKGROUND OF THE INVENTION.
- [0004] Chitosan can be created by N-deacetylation of the chitin polymer.
- BRIEF SUMMARY OF THE INVENTION.
- Compositions of the present invention further include synthetic polynucleotides encoding enzymes or polypeptides involved in the production of chitin and/or chitosan.
- sequences encoding variants and fragments of the amino acid sequence shown in SEQ ID NOS and 44..
- BRIEF DESCRIPTION OF THE DRAWING.
- DETAILED DESCRIPTION OF THE INVENTION I.
- For example, the processivity of the enzyme (i.e., the average chain length.
- controls the sugar composition of the polymer formed.
- Chitin synthase from a variety of organisms are known in the art and are contemplated for use in the genetic engineering strategies of the present invention.
- deacetylases from a variety of organisms are known in the art and are contemplated for use in the genetic engineering strategies of the present invention.
- microorganism or plant changes the ability of the microorganism or plant to produce chitin and/or chitosan.
- For purposes of the invention, "isolated".
- [0047] Preferred chitin/chitosan-related proteins of the present invention are encoded by a nucleotide sequence sufficiently identical to the nucleotide sequence of SEQ ID NOS or 43.
- BLASTX program, score=50, wordlength=3, to obtain amino acid sequences homologous to chitin/chitosan-related protein molecules of the invention.
- of the chitin/chitosan-related nucleotide sequences include those sequences that encode a.
- [0055] Using methods such as PCR, hybridization, and the like, corresponding chitin/chitosan- related sequences can be identified, such sequences having substantial identity to the sequences of the invention.
- [0056] In a hybridization method, all or part of the chitin/chitosan-related nucleotide sequence can be used to screen cDNA or genomic libraries.
- By controlling the stringency of the.
- thus, T.sub.m, hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity.
- Variant polypeptides encompassed by the present invention are biologically active, that is they continue to possess the desired biological activity of the native protein, that is, production of chitin and/or chitosan.
- [0065] Methods of the invention involve introducing one or more chitin/chitosan-related polynucleotides into a microorganism or plant.
- polynucleotide gains access to the interior of at least one cell of the microorganism or plant..
- overexpression of the enzymes.
- For example, the gene can be engineered to contain a signal peptide to facilitate transfer of the peptide to the vacuole.
- Generally, the GC content of the gene will be increased.
- [0076] In yet another embodiment of the invention, chitin/chitosan production is targeted to the vacuole, for example, by targeting the chitin and/or chitosan synthase to the v.
- For example, amino acid sequence variants of the targeted enzymes can be prepared by mutations in the polynucleotides encoding the amino acid sequence.
- In some aspects, the changes encoded in the amino acid sequence will not substantially affect the function of the protein.
- synthase that allows production of chitin as an insoluble polymer within at least one cell of the microorganism.
- [0107] As noted above, in the method for production of chitin and/or chitosan of the present invention, a microorganism or plant having a genetically modified chitin/chitosan pathway is cultured in a fermentation medium for production of chitin and/or chitosan.
- microorganism or plant of the present invention, when cultured, is capable of producing (accumulating) chitin and/or chitosan.
- [0109] Microorganism or plants of the present invention can be cultured in conventional fermentation bioreactors.
- Preferably, microorganism or plants of the present invention are grown by batch or fed-batch fermentation processes..
- It is noted that the optimum temperature for growth and chitin and/or chitosan production by a microorganism or plant of the present.
- One of ordinary skill in the art can readily determine the optimum growth and chitin and/or chitosan production temperature for any microorganism or plant of the present invention using standard techniques..
- refers to a purity that allows for the effective use of the chitin and/or chitosan as a compound for commercial sale or use.
- seeds, plant cells, propagules, embryos and progeny of the same.
- [0125] The chitin/chitosan-related genes of the invention may be modified to obtain or enhance expression in plant cells.
- The chitin/chitosan-related sequences of the invention may be provided in expression cassettes for expression in the plant of interest.
- [0130] Methods of the invention involve introducing a nucleotide construct into a plant.
- Expression of RNA encoded by polynucleotide sequences involved in the production of chitin and/or chitosan is then tested by hybridizing the filter to a radioactive probe derived from a polynucleotide of the invention, by methods known in the art (Sambrook and Russell, 2001, supra).
- aga aat gaa tat cac tca aac cgg aag aat gaa cct tcc tat 523 Arg Ser Arg Asn Glu Tyr His Ser Asn Arg Lys Asn Glu Pro Ser Tyr 10 15 20 gaa ctc caa aat gca cat agc ggg cta ttt cac tct tct aat gaa gaa 571 Glu Leu Gln Asn Ala His Ser Gly Leu Phe His Ser Ser Asn Glu Glu 25 30 35 tta aca aac agg aac caa aga tat acc aat caa aat gcc agc atg ggt 619 Leu Thr Asn Arg Asn Gln Arg Tyr Thr Asn Gln Asn Ala Ser Met Gly 40 45 50 tca ttc act cca gtc caa tct ttg caa ttt cca gaa caa tct cag caa 667 Ser Phe Thr Pro Val Gln Ser Leu Gln Phe Pro Glu Gln Ser Gln Gln aca aat atg ctt tat aac ggt gac gat ggc aat aat aat act atc aat 715 Thr Asn Met Leu Tyr Asn Gly Asp Asp Gly Asn Asn Asn Thr Ile Asn 75 80 85 gat aac gaa cga gac ata tat gga ggt ttt gtc aac cac cat cgc cag 763 Asp Asn Glu Arg Asp Ile Tyr Gly Gly Phe Val Asn His His Arg Gln 90 95 100 cgt ccc cca cca gca act gca gaa tac aat gac gtt ttt aat acg aat 811 Arg Pro Pro Pro Ala Thr Ala Glu Tyr Asn Asp Val Phe Asn Thr Asn agt caa cag cta ccg tcg gaa cat caa tac aat aac gta cct tca tat 859 Ser Gln Gln Leu Pro Ser Glu His Gln Tyr Asn Asn Val Pro Ser Tyr cca ctt cct tcg ata aat gtg att caa acc act cca gaa ctc ata cat 907 Pro Leu Pro Ser Ile Asn Val Ile Gln Thr Thr Pro Glu Leu Ile His aac ggc tca cag act atg gcc acc ccc atc gaa agg ccc ttc ttt aac 955 Asn Gly Ser Gln Thr Met Ala Thr Pro Ile Glu Arg Pro Phe Phe Asn gaa aac gac tac tat tat aat aac agg aac tct agg acg tca ccg agt 1003 Glu Asn Asp Tyr Tyr Tyr Asn Asn Arg Asn Ser Arg Thr Ser Pro Ser att gct tct agt agc gat ggt tat gca gat cag gaa gct agg ccc att 1051 Ile Ala Ser Ser Ser Asp Gly Tyr Ala Asp Gln Glu Ala Arg Pro Ile ttg gag caa ccc aac aat aac atg aat agc ggt aat att cct caa tac 1099 Leu Glu Gln Pro Asn Asn Asn Met Asn Ser Gly Asn Ile Pro Gln Tyr cat gac caa cct ttt gga tac aac aat ggt tac cat ggc cta cag gca 1147 His Asp Gln Pro Phe Gly Tyr Asn Asn Gly Tyr His Gly Leu Gln Ala aaa gat tac tat gac gat ccg gag ggt ggt tat att gat cag aga gga 1195 Lys Asp Tyr Tyr Asp Asp Pro Glu Gly Gly Tyr Ile Asp Gln Arg Gly gat gac tat cag att aat tca tat ttg ggt aga aac ggt gaa atg gtt 1243 Asp Asp Tyr Gln Ile Asn Ser Tyr Leu Gly Arg Asn Gly Glu Met Val gat cct tac gat tat gaa aac agt tta aga cat atg act cct atg gag 1291 Asp Pro Tyr Asp Tyr Glu Asn Ser Leu Arg His Met Thr Pro Met Glu cgt aga gaa tat ctt cat gat gat agc aga ccc gta aac gat gga aaa 1339 Arg Arg Glu Tyr Leu His Asp Asp Ser Arg Pro Val Asn Asp Gly Lys gaa gaa tta gac agt gtg aaa agc ggt tac tct cat aga gac ttg ggg 1387 Glu Glu Leu Asp Ser Val Lys Ser Gly Tyr Ser His Arg Asp Leu Gly gaa tat gac aag gat gat ttt tca agg gat gac gag tac gat gat ctc 1435 Glu Tyr Asp Lys Asp Asp Phe Ser Arg Asp Asp Glu Tyr Asp Asp Leu aac act att gat aaa tta cag ttt caa gct aat ggt gta cct gca tca 1483 Asn Thr Ile Asp Lys Leu Gln Phe Gln Ala Asn Gly Val Pro Ala Ser tcc tcg gtg tct tct atc gga tct aaa gaa tcc gac ata ata gta agc 1531 Ser Ser Val Ser Ser Ile Gly Ser Lys Glu Ser Asp Ile Ile Val Ser aat gat aac tta acc gca aat aga gca cta aag aga agc ggt act gaa 1579 Asn Asp Asn Leu Thr Ala Asn Arg Ala Leu Lys Arg Ser Gly Thr Glu att agg aaa ttc aaa ctt tgg aat ggt aat ttt gtt ttc gat tct cca 1627 Ile Arg Lys Phe Lys Leu Trp Asn Gly Asn Phe Val Phe Asp Ser Pro atc agt aag acg cta ttg gac caa tac gct act aca aca gaa aat gca 1675 Ile Ser Lys Thr Leu Leu Asp Gln Tyr Ala Thr Thr Thr Glu Asn Ala aac act tta cca aat gag ttt aag ttt atg aga tat caa gca gtt act 1723 Asn Thr Leu Pro Asn Glu Phe Lys Phe Met Arg Tyr Gln Ala Val Thr tgc gaa cct aat caa ctt gca gag aag aat ttc acg gtg agg cag ttg 1771 Cys Glu Pro Asn Gln Leu Ala Glu Lys Asn Phe Thr Val Arg Gln Leu aag tat tta act cca agg gaa acg gaa ttg atg cta gta gtc aca atg 1819 Lys Tyr Leu Thr Pro Arg Glu Thr Glu Leu Met Leu Val Val Thr Met tat aat gaa gac cat atc ctg tta gga aga act ttg aaa ggt att atg 1867 Tyr Asn Glu Asp His Ile Leu Leu Gly Arg Thr Leu Lys Gly Ile Met gac aat gtc aaa tat atg gtg aaa aaa aaa aat tca agc act tgg ggg 1915 Asp Asn Val Lys Tyr Met Val Lys Lys Lys Asn Ser Ser Thr Trp Gly ccg gat gca tgg aaa aag att gtc gtt tgt atc.
- att tca gat ggt aga 1963 Pro Asp Ala Trp Lys Lys Ile Val Val Cys Ile Ile Ser Asp Gly Arg tcc aaa att aat gaa cgc tcg cta gca tta cta agt tcg tta ggt tgt 2011 Ser Lys Ile Asn Glu Arg Ser Leu Ala Leu Leu Ser Ser Leu Gly Cys tac cag gac ggg ttt gct aag gat gaa att aat gaa aaa aaa gtg gca 2059 Tyr Gln Asp Gly Phe Ala Lys Asp Glu Ile Asn Glu Lys Lys Val Ala atg cat gtc tac gaa cat acg aca atg atc aac atc aca aat att tcg 2107 Met His Val Tyr Glu His Thr Thr Met Ile Asn Ile Thr Asn Ile Ser gaa tca gag gtt tca tta gaa tgc aat caa ggt acc gtt cca ata caa 2155 Glu Ser Glu Val Ser Leu Glu Cys Asn Gln Gly Thr Val Pro Ile Gln ctt ttg ttt tgt ttg aaa gag caa aat cag aaa aaa att aac tca cat 2203 Leu Leu Phe Cys Leu Lys Glu Gln Asn Gln Lys Lys Ile Asn Ser His aga tgg gca ttt gaa ggc ttt gca gaa tta ctg cgt ccc aat atc gtt 2251 Arg Trp Ala Phe Glu Gly Phe Ala Glu Leu Leu Arg Pro Asn Ile Val aca ttg tta gat gct ggt acc atg cca ggt aaa gat tct att tac cag 2299 Thr Leu Leu Asp Ala Gly Thr Met Pro Gly Lys Asp Ser Ile Tyr Gln tta tgg aga gag ttc agg aat cca aat gtt ggt ggc gca tgt ggt gaa 2347 Leu Trp Arg Glu Phe Arg Asn Pro Asn Val Gly Gly Ala Cys Gly Glu ata aga act gat ttg ggt aag aga ttt gta aag ctt ttg aat cct tta 2395 Ile Arg Thr Asp Leu Gly Lys Arg Phe Val Lys Leu Leu Asn Pro Leu gtt gca tca cag aat ttc gaa tac aaa atg tcc aat att tta gac aaa 2443 Val Ala Ser Gln Asn Phe Glu Tyr Lys Met Ser Asn Ile Leu Asp Lys aca acc gag tct aac ttt gga ttt att act gtt cta ccg ggg gca ttc 2491 Thr Thr Glu Ser Asn Phe Gly Phe Ile Thr Val Leu Pro Gly Ala Phe tct gcg tat agg ttt gaa gct gtg aga ggc caa cca tta cag aag tac 2539 Ser Ala Tyr Arg Phe Glu Ala Val Arg Gly Gln Pro Leu Gln Lys Tyr ttt tat ggt gaa att atg gaa aat gaa ggt ttt cat ttt ttt tct tcc 2587 Phe Tyr Gly Glu Ile Met Glu Asn Glu Gly Phe His Phe Phe Ser Ser aat atg tat ctt gct gaa gat cgt att tta tgc ttt gaa gtg gtc aca 2635 Asn Met Tyr Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Val Val Thr aaa aaa aat tgt aat tgg att ttg aaa tac tgc aga agt tct tat gct 2683 Lys Lys Asn Cys Asn Trp Ile Leu Lys Tyr Cys Arg Ser Ser Tyr Ala tca aca gat gta ccg gag agg gtc cct gaa ttt att ctt cag agg agg 2731 Ser Thr Asp Val Pro Glu Arg Val Pro Glu Phe Ile Leu Gln Arg Arg cgt tgg ttg aat ggt tca ttt ttt gct agt gta tat tcc ttt tgt cat 2779 Arg Trp Leu Asn Gly Ser Phe Phe Ala Ser Val Tyr Ser Phe Cys His ttt tac aga gtc tgg agc agt ggt cat aat att ggt aga aaa ctc ctt 2827 Phe Tyr Arg Val Trp Ser Ser Gly His Asn Ile Gly Arg Lys Leu Leu ttg acg gtt gaa ttt ttt tac ctt ttc ttc aat aca ttg att tca tgg 2875 Leu Thr Val Glu Phe Phe Tyr Leu Phe Phe Asn Thr Leu Ile Ser Trp ttt tca ttg agt tca ttt ttc cta ttc ttt aga att ctc act gtt tct 2923 Phe Ser Leu Ser Ser Phe Phe Leu Phe Phe Arg Ile Leu Thr Val Ser att gca ctg gca tac cat tca gca ttt aat gtg ttg tcc gtc ata ttc 2971 Ile Ala Leu Ala Tyr His Ser Ala Phe Asn Val Leu Ser Val Ile Phe ctg tgg ctt tat ggg att tgt acc tta tca aca ttc ata ctg tca ttg 3019 Leu Trp Leu Tyr Gly Ile Cys Thr Leu Ser Thr Phe Ile Leu Ser Leu ggt aat aaa cct aaa agt act gag aaa ttt tat gtt cta act tgc gtc 3067 Gly Asn Lys Pro Lys Ser Thr Glu Lys Phe Tyr Val Leu Thr Cys Val att ttt gcg gtg atg atg att tac atg ata ttc tgc agt ata ttc atg 3115 Ile Phe Ala Val Met Met Ile Tyr Met Ile Phe Cys Ser Ile Phe Met agt gtc aaa tcc ttc caa aat ata ttg aaa aac gat acc atc agc ttt 3163 Ser Val Lys Ser Phe Gln Asn Ile Leu Lys Asn Asp Thr Ile Ser Phe gag ggt ttg att acc aca gaa gct ttc agg gat att gtt atc tct ctg 3211 Glu Gly Leu Ile Thr Thr Glu Ala Phe Arg Asp Ile Val Ile Ser Leu ggc tcc act tat tgt ttg tac cta atc agt tca att atc tat ttg cag 3259 Gly Ser Thr Tyr Cys Leu Tyr Leu Ile Ser Ser Ile Ile Tyr Leu Gln cca tgg cat atg ttg aca agt ttt att cag tat att tta ttg agt cct 3307 Pro Trp His Met Leu Thr Ser Phe Ile Gln Tyr Ile Leu Leu Ser Pro tct tac atc aat gtt ttg aat atc tat gca ttt tgt aat gtc cac gac 3355 Ser Tyr Ile Asn Val Leu Asn Ile Tyr Ala Phe Cys Asn Val His Asp tta tca tgg ggt aca aag ggt gca atg gca aat ccg ctg ggt aag att 3403 Leu Ser Trp Gly Thr Lys Gly Ala Met Ala Asn Pro Leu Gly Lys Ile 970.
- 975 980 aat act aca gaa gat ggt acg ttc aaa atg gaa gtt ctg gtc tct agt 3451 Asn Thr Thr Glu Asp Gly Thr Phe Lys Met Glu Val Leu Val Ser Ser tca gag att caa gca aac tac gat aaa tat ttg aaa gtt tta aat gac 3499 Ser Glu Ile Gln Ala Asn Tyr Asp Lys Tyr Leu Lys Val Leu Asn Asp ttc gat cca aaa tca gaa tct cgg cct act gag cca tct tat gat gaa 3547 Phe Asp Pro Lys Ser Glu Ser Arg Pro Thr Glu Pro Ser Tyr Asp Glu aaa aag act ggc tat tat gca aac gtt aga tct ctc gtg att atc ttt 3595 Lys Lys Thr Gly Tyr Tyr Ala Asn Val Arg Ser Leu Val Ile Ile Phe tgg gtc atc aca aat ttc atc atc gtt gct gtt gtc tta gaa acc ggt 3643 Trp Val Ile Thr Asn Phe Ile Ile Val Ala Val Val Leu Glu Thr Gly ggg att gca gat tat att gct atg aaa tcc ata tca act gat gac act 3691 Gly Ile Ala Asp Tyr Ile Ala Met Lys Ser Ile Ser Thr Asp Asp Thr tta gaa act gca aag aag gcg gaa att ccc tta atg acc agt aag gcc 3739 Leu Glu Thr Ala Lys Lys Ala Glu Ile Pro Leu Met Thr Ser Lys Ala tca att tat ttt aat gta att tta tgg tta gtt gca tta tcg gca tta 3787 Ser Ile Tyr Phe Asn Val Ile Leu Trp Leu Val Ala Leu Ser Ala Leu ata agg ttc att ggt tgc tca ata tac atg ata gta agg ttt ttt aaa 3835 Ile Arg Phe Ile Gly Cys Ser Ile Tyr Met Ile Val Arg Phe Phe Lys aag gtt aca ttt cgc taa gtagattgat tacccctttg ttataaataa 3883 Lys Val Thr Phe Arg * 1130 gtatatgtgt.
- ttactatcat ataataatta attagttgta agtatggtat ttttcaaaga 3943 aacatttagt attattttta aagggtgcct ttaccataaa accaaaaaaa aatggcttct 4003 gagagaaaaa cataaataat tgataaaact gaaactcaca acaccaactc ttccgctcta 4063 aacacccaaa gctcaaaaaa cgacatgtgc acatctatat ccataaactt tcaaaaaagt 4123 ggttcattcg caaattcatg aagtattatt taatacacag taatctggca tttcatacta 4183 tcattgctca aattatccgc cggacccttg aaactgtggc gctttttaag acgcatctcc 4243 aaaaaaagaa aaagaacaaa agaaaaaaag ctgcaaatat atatacaaaa cagatttcac 4303 gtttattgct tgggagtaag gtaacgatcc ggaagccttg aagaaatttg aatgtatcca 4363 actaactatt gaaaatcatt cgtg PRT Saccharomyces cerevisiae 2 Met Ser Asp Gln Asn Asn Arg Ser Arg Asn Glu Tyr His Ser Asn Arg 1 5 10 15 Lys Asn Glu Pro Ser Tyr Glu Leu Gln Asn Ala His Ser Gly Leu Phe 20 25 30 His Ser Ser Asn Glu Glu Leu Thr Asn Arg Asn Gln Arg Tyr Thr Asn 35 40 45 Gln Asn Ala Ser Met Gly Ser Phe Thr Pro Val Gln Ser Leu Gln Phe 50 55 60 Pro Glu Gln Ser Gln Gln Thr Asn Met Leu Tyr Asn Gly Asp Asp Gly Asn Asn Asn Thr Ile Asn Asp Asn Glu Arg Asp Ile Tyr Gly Gly Phe 85 90 95 Val Asn His His Arg Gln Arg Pro Pro Pro Ala Thr Ala Glu Tyr Asn Asp Val Phe Asn Thr Asn Ser Gln Gln Leu Pro Ser Glu His Gln Tyr Asn Asn Val Pro Ser Tyr Pro Leu Pro Ser Ile Asn Val Ile Gln Thr Thr Pro Glu Leu Ile His Asn Gly Ser Gln Thr Met Ala Thr Pro Ile Glu Arg Pro Phe Phe Asn Glu Asn Asp Tyr Tyr Tyr Asn Asn Arg Asn Ser Arg Thr Ser Pro Ser Ile Ala Ser Ser Ser Asp Gly Tyr Ala Asp Gln Glu Ala Arg Pro Ile Leu Glu Gln Pro Asn Asn Asn Met Asn Ser Gly Asn Ile Pro Gln Tyr His Asp Gln Pro Phe Gly Tyr Asn Asn Gly Tyr His Gly Leu Gln Ala Lys Asp Tyr Tyr Asp Asp Pro Glu Gly Gly Tyr Ile Asp Gln Arg Gly Asp Asp Tyr Gln Ile Asn Ser Tyr Leu Gly Arg Asn Gly Glu Met Val Asp Pro Tyr Asp Tyr Glu Asn Ser Leu Arg His Met Thr Pro Met Glu Arg Arg Glu Tyr Leu His Asp Asp Ser Arg Pro Val Asn Asp Gly Lys Glu Glu Leu Asp Ser Val Lys Ser Gly Tyr Ser His Arg Asp Leu Gly Glu Tyr Asp Lys Asp Asp Phe Ser Arg Asp Asp Glu Tyr Asp Asp Leu Asn Thr Ile Asp Lys Leu Gln Phe Gln Ala Asn Gly Val Pro Ala Ser Ser Ser Val Ser Ser Ile Gly Ser Lys Glu Ser Asp Ile Ile Val Ser Asn Asp Asn Leu Thr Ala Asn Arg Ala Leu Lys Arg Ser Gly Thr Glu Ile Arg Lys Phe Lys Leu Trp Asn Gly Asn Phe Val Phe Asp Ser Pro Ile Ser Lys Thr Leu Leu Asp Gln Tyr Ala Thr Thr Thr Glu Asn Ala Asn Thr Leu Pro Asn Glu Phe Lys Phe Met Arg Tyr Gln Ala Val Thr Cys Glu Pro Asn Gln Leu Ala Glu Lys Asn .
- Phe Thr Val Arg Gln Leu Lys Tyr Leu Thr Pro Arg Glu Thr Glu Leu Met Leu Val Val Thr Met Tyr Asn Glu Asp His Ile Leu Leu Gly Arg Thr Leu Lys Gly Ile Met Asp Asn Val Lys Tyr Met Val Lys Lys Lys Asn Ser Ser Thr Trp Gly Pro Asp Ala Trp Lys Lys Ile Val Val Cys Ile Ile Ser Asp Gly Arg Ser Lys Ile Asn Glu Arg Ser Leu Ala Leu Leu Ser Ser Leu Gly Cys Tyr Gln Asp Gly Phe Ala Lys Asp Glu Ile Asn Glu Lys Lys Val Ala Met His Val Tyr Glu His Thr Thr Met Ile Asn Ile Thr Asn Ile Ser Glu Ser Glu Val Ser Leu Glu Cys Asn Gln Gly Thr Val Pro Ile Gln Leu Leu Phe Cys Leu Lys Glu Gln Asn Gln Lys Lys Ile Asn Ser His Arg Trp Ala Phe Glu Gly Phe Ala Glu Leu Leu Arg Pro Asn Ile Val Thr Leu Leu Asp Ala Gly Thr Met Pro Gly Lys Asp Ser Ile Tyr Gln Leu Trp Arg Glu Phe Arg Asn Pro Asn Val Gly Gly Ala Cys Gly Glu Ile Arg Thr Asp Leu Gly Lys Arg Phe Val Lys Leu Leu Asn Pro Leu Val Ala Ser Gln Asn Phe Glu Tyr Lys Met Ser Asn Ile Leu Asp Lys Thr Thr Glu Ser Asn Phe Gly Phe Ile Thr Val Leu Pro Gly Ala Phe Ser Ala Tyr Arg Phe Glu Ala Val Arg Gly Gln Pro Leu Gln Lys Tyr Phe Tyr Gly Glu Ile Met Glu Asn Glu Gly Phe His Phe Phe Ser Ser Asn Met Tyr Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Val Val Thr Lys Lys Asn Cys Asn Trp Ile Leu Lys Tyr Cys Arg Ser Ser Tyr Ala Ser Thr Asp Val Pro Glu Arg Val Pro Glu Phe Ile Leu Gln Arg Arg Arg Trp Leu Asn Gly Ser Phe Phe Ala Ser Val Tyr Ser Phe Cys His Phe Tyr Arg Val Trp Ser Ser Gly His Asn Ile Gly Arg Lys Leu Leu Leu Thr Val Glu Phe Phe Tyr Leu Phe Phe Asn Thr Leu Ile Ser Trp Phe Ser Leu Ser Ser Phe Phe Leu Phe Phe Arg Ile Leu Thr Val Ser Ile Ala Leu Ala Tyr His Ser Ala Phe Asn Val Leu Ser Val Ile Phe Leu Trp Leu Tyr Gly Ile Cys Thr Leu Ser Thr Phe Ile Leu Ser Leu Gly Asn Lys Pro Lys Ser Thr Glu Lys Phe Tyr Val Leu Thr Cys Val Ile Phe Ala Val Met Met Ile Tyr Met Ile Phe Cys Ser Ile Phe Met Ser Val Lys Ser Phe Gln Asn Ile Leu Lys Asn Asp Thr Ile Ser Phe Glu Gly Leu Ile Thr Thr Glu Ala Phe Arg Asp Ile Val Ile Ser Leu Gly Ser Thr Tyr Cys Leu Tyr Leu Ile Ser Ser Ile Ile Tyr Leu Gln Pro Trp His Met Leu Thr Ser Phe Ile Gln Tyr Ile Leu Leu Ser Pro Ser Tyr Ile Asn Val Leu Asn Ile Tyr Ala Phe Cys Asn Val His Asp Leu Ser Trp Gly Thr Lys Gly Ala Met Ala Asn Pro Leu Gly Lys Ile Asn Thr Thr Glu Asp Gly Thr Phe Lys Met Glu Val Leu Val Ser Ser Ser Glu Ile Gln Ala Asn Tyr Asp Lys Tyr Leu Lys Val Leu Asn Asp Phe Asp Pro Lys Ser Glu Ser Arg Pro Thr Glu Pro Ser Tyr Asp Glu Lys Lys Thr Gly Tyr Tyr Ala Asn Val Arg Ser Leu Val Ile Ile Phe Trp Val Ile Thr Asn Phe Ile Ile Val Ala Val Val Leu Glu Thr Gly Gly Ile Ala Asp Tyr Ile Ala Met Lys Ser Ile Ser Thr Asp Asp Thr Leu Glu Thr Ala Lys Lys Ala Glu Ile Pro Leu Met Thr Ser Lys Ala Ser Ile Tyr Phe Asn Val Ile Leu Trp Leu Val Ala Leu Ser Ala Leu Ile Arg Phe Ile Gly Cys Ser Ile Tyr Met Ile Val Arg Phe Phe Lys Lys Val Thr Phe Arg DNA Saccharomyces cerevisiae 3 ctt ttc tac gtt tgc tgt ttt ttc tgt ttc cct tgc ctt ata cga aca 48 tac gtt tct aat gcc gat tat aaa cac aat aga cac aat tat act att 96 ttc tca ctt ttc tct tcg gga gat cgc tat tga tta tta ttc ccg ttt 144 att tcc ttc aat tta ggg tct gaa aag aag ata gta ggg tta gtt ttt 192 tgg ggt tta aaa ccc tag tat aaa ccc cta tac tat ttg ttt ata tat 240 ttc cta ttt gag tat gat agc atc gat att gaa att tac aga act tca 288 att tag ttt caa ttg gga att cga gga ctc tcc ctt ggg atg gcc cca 336 gtc tat tta act atc ttc cat aat att act gtt gat ttc gtg act cag 384 aag aga aaa cag agg aag tta cat ata gac cca aat aaa aac caa aga 432 acc aca tat aga aat gac gag aaa ccc gtt tat ggt gga acc ttc gaa 480 tgg ctc tcc taa tag acg tgg tgc ttc aaa cct ctc caa att tta cgc 528 aaa cgc taa cag caa ctc tcg gtg ggc taa tcc cag tga gga gag ttt 576 gga gga tag cta tga cca atc taa cgt ttt cca agg cct tcc ggc atc 624 tcc ttc.
- cga gta act acg cga gga acc cgg acc gca ata acg att aaa gaa 3984 ggc ccg gaa ggg aga tgc tta aat gat tat cac tca gtt aaa aaa gac 4032 aaa taa gaa act att gag act gaa ccg ttt tgg tta att tca ggt gga 4080 aac aat tga aga cga gca gta aac att att tta ttt agt agt c PRT Saccharomyces cerevisiae 4 Met Thr Arg Asn Pro Phe Met Val Glu Pro Ser Asn Gly Ser Pro Asn 1 5 10 15 Arg Arg Gly Ala Ser Asn Leu Ser Lys Phe Tyr Ala Asn Ala Asn Ser 20 25 30 Asn Ser Arg Trp Ala Asn Pro Ser Glu Glu Ser Leu Glu Asp Ser Tyr 35 40 45 Asp Gln Ser Asn Val Phe Gln Gly Leu Pro Ala Ser Pro Ser Arg Ala 50 55 60 Ala Leu Arg Tyr Ser Pro Asp Arg Arg His Arg Thr Gln Phe Tyr Arg Asp Ser Ala His Asn Ser Pro Val Ala Pro Asn Arg Tyr Ala Ala Asn 85 90 95 Leu Gln Glu Ser Pro Lys Arg Ala Gly Glu Ala Val Ile His Leu Ser Glu Gly Ser Asn Leu Tyr Pro Arg Asp Asn Ala Asp Leu Pro Val Asp Pro Tyr His Leu Ser Pro Gln Gln Gln Pro Ser Asn Asn Leu Phe Gly Ser Gly Arg Leu Tyr Ser Gln Ser Ser Lys Tyr Thr Met Ser Thr Thr Ser Thr Thr Ala Pro Ser Leu Ala Glu Ala Asp Asp Glu Lys Glu Lys Tyr Leu Thr Ser Thr Thr Ser Tyr Asp Asp Gln Ser Thr Ile Phe Ser Ala Asp Thr Phe Asn Glu Thr Lys Phe Glu Leu Asn His Pro Thr Arg Gln Gln Tyr Val Arg Arg Ala Asn Ser Glu Ser Lys Arg Arg Met Val Ser Asp Leu Pro Pro Pro Ser Lys Lys Lys Ala Leu Leu Lys Leu Asp Asn Pro Ile Pro Lys Gly Leu Leu Asp Thr Leu Pro Arg Arg Asn Ser Pro Glu Phe Thr Glu Met Arg Tyr Thr Ala Cys Thr Val Glu Pro Asp Asp Phe Leu Arg Glu Gly Tyr Thr Leu Arg Phe Ala Glu Met Asn Arg Glu Cys Gln Ile Ala Ile Cys Ile Thr Met Tyr Asn Glu Asp Lys Tyr Ser Leu Ala Arg Thr Ile His Ser Ile Met Lys Asn Val Ala His Leu Cys Lys Arg Glu Lys Ser His Val Trp Gly Pro Asn Gly Trp Lys Lys Val Ser Val Ile Leu Ile Ser Asp Gly Arg Ala Lys Val Asn Gln Gly Ser Leu Asp Tyr Leu Ala Ala Leu Gly Val Tyr Gln Glu Asp Met Ala Lys Ala Ser Val Asn Gly Asp Pro Val Lys Ala His Ile Phe Glu Leu Thr Thr Gln Val Ser Ile Asn Ala Asp Leu Asp Tyr Val Ser Lys Asp Ile Val Pro Val Gln Leu Val Phe Cys Leu Lys Glu Glu Asn Lys Lys Lys Ile Asn Ser His Arg Trp Leu Phe Asn Ala Phe Cys Pro Val Leu Gln Pro Thr Val Val Thr Leu Val Asp Val Gly Thr Arg Leu Asn Asn Thr Ala Ile Tyr Arg Leu Trp Lys Val Phe Asp Met Asp Ser Asn Val Ala Gly Ala Ala Gly Gln Ile Lys Thr Met Lys Gly Lys Trp Gly Leu Lys Leu Phe Asn Pro Leu Val Ala Ser Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu Asp Lys Pro Leu Glu Ser Val Phe Gly Tyr Ile Ser Val Leu Pro Gly Ala Leu Ser Ala Tyr Arg Tyr Arg Ala Leu Lys Asn His Glu Asp Gly Thr Gly Pro Leu Arg Ser Tyr Phe Leu Gly Glu Thr Gln Glu Gly Arg Asp His Asp Val Phe Thr Ala Asn Met Tyr Leu Ala Glu Asp Arg Ile Leu Cys Trp Glu Leu Val Ala Lys Arg Asp Ala Lys Trp Val Leu Lys Tyr Val Lys Glu Ala Thr Gly Glu Thr Asp Val Pro Glu Asp Val Ser Glu Phe Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ala Met Phe Ala Ala Ile Tyr Ala Gln Leu His Phe Tyr Gln Ile Trp Lys Thr Lys His Ser Val Val Arg Lys Phe Phe Leu His Val Glu Phe Leu Tyr Gln Phe Ile Gln Met Leu Phe Ser Trp Phe Ser Ile Ala Asn Phe Val Leu Thr Phe Tyr Tyr Leu Ala Gly Ser Met Asn Leu Val Ile Lys His Gly Glu Ala Leu Phe Ile Phe Phe Lys Tyr Leu Ile Phe Cys Asp Leu Ala Ser Leu Phe Ile Ile Ser Met Gly Asn Arg Pro Gln Gly Ala Lys His Leu Phe Ile Thr Ser Met Val Ile Leu Ser Ile Cys Ala Thr Tyr Ser Leu Ile Cys Gly Phe Val Phe Ala Phe Lys Ser Leu Ala Ser Gly Thr Glu Ser His Lys Ile Phe Val Asp Ile Val Ile Ser Leu Leu Ser Thr Tyr Gly Leu Tyr Phe Phe Ser Ser Leu Met Tyr Leu Asp Pro Trp His Met Phe Thr Ser Ser Ile Gln Tyr Phe Leu Thr Leu Pro Ala Phe Thr Cys Thr Leu Gln Ile Phe Ala Phe Cys Asn Thr His Asp Val Ser Trp Gly Thr Lys Gly Ser Thr Gln Glu Ser Lys Gln Leu Ser 805 810.
- 815 Lys Ala Ile Val Val Gln Gly Pro Asp Gly Lys Gln Ile Val Glu Thr Asp Trp Pro Gln Glu Val Asp Lys Lys Phe Leu Glu Ile Lys Ser Arg Leu Lys Glu Pro Glu Phe Glu Glu Ser Ser Gly Asn Glu Lys Gln Ser Lys Asn Asp Tyr Tyr Arg Asp Ile Arg Thr Arg Ile Val Met Ile Trp Met Leu Ser Asn Leu Ile Leu Ile Met Ser Ile Ile Gln Val Phe Thr Pro Gln Asp Thr Asp Asn Gly.
- Tyr Leu Ile Phe Ile Leu Trp Ser Val Ala Ala Leu Ala Ala Phe Arg Val Val Gly Ser Met Ala Phe Leu Phe Met Lys Tyr Leu Arg Ile Ile Val Ser Tyr Arg Asn Lys Val Glu Gly Ser Gly Ser Trp Glu Val Ser Lys Leu Asp Leu Pro Asn Val Phe His Lys Lys Gly 5 5176 DNA Saccharomyces cerevisiae 5 taattgcctt agccacgtaa tatgtgcctt.
- aaacctaaca aagtatgaat ttcgagaagt cccatcgata 5057 gtagtcaaac aattgctaaa gagggctacg gatcgtctac aataccttcg taaaacagca 5117 gcttacagga tcttttataa tatcaccata gaaaggggaa tttgggaagc gagagtaaa PRT Saccharomyces cerevisiae 6 Met Thr Gly Leu Asn Gly Asp Asp Pro Asp Asp Tyr Tyr Leu Asn Leu 1 5 10 15 Asn Gln Asp Glu Glu Ser Leu Leu Arg Ser Arg His Ser Val Gly Ser 20 25 30 Gly Ala Pro His Arg Gln Gly Ser Leu Val Arg Pro Glu Arg Ser Arg 35 40 45 Leu Asn Asn Pro Asp Asn Pro His Phe Tyr Tyr Ala Gln Lys Thr Gln 50 55 60 Glu Gln Met Asn His Leu Asp Val Leu Pro Ser Ser Thr Gly Val Asn Pro Asn Ala Thr Arg Arg Ser Gly Ser Leu Arg Ser Lys Gly Ser Val 85 90 95 Arg Ser Lys Phe Ser Gly Arg Glu Thr Asp Ser Tyr Leu Leu Gln Asp Met Asn Thr Thr Asp Lys Lys Ala Ser Val Lys Ile Ser Asp Glu Gly Val Ala Glu Asp Glu Phe Asp Lys Asp Gly Asp Val Asp Asn Phe Glu Glu Ser Ser Thr Gln Pro Ile Asn Lys Ser Ile Lys Pro Leu Arg Lys Glu Thr Asn Asp Thr Leu Ser Phe Trp Gln Met Tyr Cys Tyr Phe Ile Thr Phe Trp Ala Pro Ala Pro Ile Leu Ala Phe Cys Gly Met Pro Lys Lys Glu Arg Gln Met Ala Trp Arg Glu Lys Val Ala Leu Ile Ser Val Ile Leu Tyr Ile Gly Ala Ile Val Ala Phe Leu Thr Phe Gly Phe Thr Lys Thr Val Cys Ser Ser Ser Lys Leu Arg Leu Lys Asn Asn Glu Val 225 230.
- 235 240 Ser Thr Glu Phe Val Val Ile Asn Gly Lys Ala Tyr Glu Leu Asp Thr Ser Ser Arg Ser Gly Ile Gln Asp Val Glu Val Asp Ser Asp Thr Leu Tyr Gly Pro Trp Ser Asp Ala Gly Lys Asp Ala Ser Phe Leu Phe Gln Asn Val Asn Gly Asn Cys His Asn Leu Ile Thr Pro Lys Ser Asn Ser Ser Ile Pro His Asp Asp Asp Asn Asn Leu Ala Trp Tyr Phe Pro Cys Lys Leu Lys Asn Gln Asp Gly Ser Ser Lys Pro Asn Phe Thr Val Glu Asn Tyr Ala Gly Trp Asn Cys His Thr Ser Lys Glu Asp Arg Asp Ala Phe Tyr Gly Leu Lys Ser Lys Ala Asp Val Tyr Phe Thr Trp Asp Gly Ile Lys Asn Ser Ser Arg Asn Leu Ile Val Tyr Asn Gly Asp Val Leu Asp Leu Asp Leu Leu Asp Trp Leu Glu Lys Asp Asp Val Asp Tyr Pro Val Val Phe Asp Asp Leu Lys Thr Ser Asn Leu Gln Gly Tyr Asp Leu Ser Leu Val Leu Ser Asn Gly His Glu Arg Lys Ile Ala Arg Cys Leu Ser Glu Ile Ile Lys Val Gly Glu Val Asp Ser Lys Thr Val Gly Cys Ile Ala Ser Asp Val Val Leu Tyr Val Ser Leu Val Phe Ile Leu Ser Val Val Ile Ile Lys Phe Ile Ile Ala Cys Tyr Phe Arg Trp Thr Val Ala Arg Lys Gln Gly Ala Tyr Ile Val Asp Asn Lys Thr Met Asp Lys His Thr Asn Asp Ile Glu Asp Trp Ser Asn Asn Ile Gln Thr Lys Ala Pro Leu Lys Glu Val Asp Pro His Leu Arg Pro Lys Lys Tyr Ser Lys Lys Ser Leu Gly His Lys Arg Ala Ser Thr Phe Asp Leu Leu Lys Lys His Ser Ser Lys Met Phe Gln Phe Asn Glu Ser Val Ile Asp Leu Asp Thr Ser Met Ser Ser Ser Leu Gln Ser Ser Gly Ser Tyr Arg Gly Met Thr Thr Met Thr Thr Gln Asn Ala Trp Lys Leu Ser Asn Glu Asn Lys Ala Val His Ser Arg Asn Pro Ser Thr Leu Leu Pro Thr Ser Ser Met Phe Trp Asn Lys Ala Thr Ser Ser Pro Val Pro Gly Ser Ser Leu Ile Gln Ser Leu Asp Ser Thr Ile Ile His Pro Asp Ile Val Gln Gln Pro Pro Leu Asp Phe Met Pro Tyr Gly Phe Pro Leu Ile His Thr Ile Cys Phe Val Thr Cys Tyr Ser Glu Asp Glu Glu Gly Leu Arg Thr Thr Leu Asp Ser Leu Ser Thr Thr Asp Tyr Pro Asn Ser His Lys Leu Leu Met Val Val Cys Asp Gly Leu Ile Lys Gly Ser Gly Asn Asp Lys Thr Thr Pro Glu Ile Ala Leu Gly Met Met Asp Asp Phe Val Thr Pro Pro Asp Glu Val Lys Pro Tyr Ser Tyr Val Ala Val Ala Ser Gly Ser Lys Arg His Asn Met Ala Lys Ile Tyr Ala Gly Phe Tyr Lys Tyr Asp Asp Ser Thr Ile Pro Pro Glu Asn Gln Gln Arg Val Pro Ile Ile Thr Ile Val Lys Cys Gly Thr Pro Ala Glu Gln Gly Ala Ala Lys Pro Gly Asn Arg Gly Lys Arg Asp Ser Gln Ile Ile Leu Met Ser Phe Leu Glu Lys Ile Thr Phe Asp Glu Arg Met Thr Gln Leu Glu Phe Gln Leu Leu Lys Asn Ile Trp Gln Ile Thr Gly Leu Met Ala Asp Phe Tyr Glu Thr Val Leu Met Val Asp Ala Asp Thr Lys Val Phe Pro Asp Ala Leu Thr His Met Val Ala Glu Met Val Lys Asp Pro Leu Ile Met Gly Leu Cys Gly Glu Thr Lys Ile Ala Asn Lys Ala Gln Ser Trp Val Thr Ala Ile Gln Val Phe Glu Tyr Tyr Ile Ser His His Gln Ala Lys Ala Phe Glu Ser Val Phe Gly Ser Val Thr Cys Leu Pro Gly Cys Phe Ser Met Tyr Arg Ile Lys Ser Pro Lys Gly Ser Asp Gly Tyr Trp Val Pro Val Leu Ala Asn Pro Asp Ile Val Glu Arg Tyr Ser Asp Asn Val Thr Asn Thr Leu His Lys Lys Asn Leu Leu Leu Leu Gly Glu Asp Arg Phe Leu Ser Ser Leu Met Leu Lys Thr Phe Pro Lys Arg Lys Gln Val Phe Val Pro Lys Ala Ala Cys Lys Thr Ile Ala Pro Asp Lys Phe Lys Val Leu Leu Ser Gln Arg Arg Arg Trp Ile Asn Ser Thr Val His Asn Leu Phe Glu Leu Val Leu Ile Arg Asp Leu Cys Gly Thr Phe Cys Phe Ser Met Gln Phe Val Ile Gly Ile Glu Leu Ile Gly Thr Met Val Leu Pro Leu Ala Ile Cys Phe Thr Ile Tyr Val Ile Ile Phe Ala Ile Val Ser Lys Pro Thr Pro Val Ile Thr Leu Val Leu Leu Ala Ile Ile Leu Gly Leu Pro Gly Leu Ile Val Val Ile Thr Ala Thr Arg Trp Ser Tyr Leu Trp Trp Met Cys Val Tyr Ile Cys Ala Leu Pro Ile Trp Asn Phe Val Leu Pro Ser Tyr Ala Tyr.
- Saccharomyces cerevisiae 8 Met Cys Gly Ile Phe Gly Tyr Cys Asn Tyr Leu Val Glu Arg Ser Arg 1 5 10 15 Gly Glu Ile Ile Asp Thr Leu Val Asp Gly Leu Gln Arg Leu Glu Tyr 20 25 30 Arg Gly Tyr Asp Ser Thr Gly Ile Ala Ile Asp Gly Asp Glu Ala Asp 35 40 45 Ser Thr Phe Ile Tyr Lys Gln Ile Gly Lys Val Ser Ala Leu Lys Glu 50 55 60 Glu Ile Thr Lys Gln Asn Pro Asn Arg Asp Val Thr Phe Val Ser His Cys Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Arg Pro Glu Gln 85 90 95 Val Asn Cys His Pro Gln Arg Ser Asp Pro Glu Asp Gln Phe Val Val Val His Asn Gly Ile Ile Thr Asn Phe Arg Glu Leu Lys Thr Leu Leu Ile Asn Lys Gly Tyr Lys Phe Glu Ser Asp Thr Asp Thr Glu Cys Ile Ala Lys Leu Tyr Leu His Leu Tyr Asn Thr Asn Leu Gln Asn Gly His Asp Leu Asp Phe His Glu Leu Thr Lys Leu Val Leu Leu Glu Leu Glu Gly Ser Tyr Gly Leu Leu Cys Lys Ser Cys His Tyr Pro Asn Glu Val Ile Ala Thr Arg Lys Gly Ser Pro Leu Leu Ile Gly Val Lys Ser Glu Lys Lys Leu Lys Val Asp Phe Val Asp Val Glu Phe Pro Glu Glu Asn Ala Gly Gln Pro Glu Ile Pro Leu Lys Ser Asn Asn Lys Ser Phe Gly Leu Gly Pro Lys Lys Ala Arg Glu Phe Glu Ala Gly Ser Gln Asn Ala Asn Leu Leu Pro Ile Ala Ala Asn Glu Phe Asn Leu Arg His Ser Gln Ser Arg Ala Phe Leu Ser Glu Asp Gly Ser Pro Thr Pro Val Glu Phe Phe Val Ser Ser Asp Ala Ala Ser Val Val Lys His Thr Lys Lys Val Leu Phe Leu Glu Asp Asp Asp Leu Ala His Ile Tyr Asp Gly Glu Leu His Ile His Arg Ser Arg Arg Glu Val Gly Ala Ser Met Thr Arg Ser Ile Gln Thr Leu Glu Met Glu Leu Ala Gln Ile Met Lys Gly Pro Tyr Asp His Phe Met Gln Lys Glu Ile Tyr Glu Gln Pro Glu Ser Thr Phe Asn Thr Met Arg Gly Arg Ile Asp Tyr Glu Asn Asn Lys Val Ile Leu Gly Gly Leu Lys Ala Trp Leu Pro Val Val Arg Arg Ala Arg Arg Leu Ile Met Ile Ala Cys Gly Thr Ser Tyr His Ser Cys Leu Ala Thr Arg Ala Ile Phe Glu Glu Leu Ser Asp Ile Pro Val Ser Val Glu Leu Ala Ser Asp Phe Leu Asp Arg Lys Cys Pro Val Phe Arg Asp Asp Val Cys Val Phe Val Ser Gln Ser Gly Glu Thr Ala Asp Thr Met Leu Ala Leu Asn Tyr Cys Leu Glu Arg Gly Ala Leu Thr Val Gly Ile Val Asn Ser Val Gly Ser Ser Ile Ser Arg Val Thr His Cys Gly Val His Ile Asn Ala Gly Pro Glu Ile Gly Val Ala Ser Thr Lys Ala Tyr Thr Ser Gln Tyr Ile Ala Leu Val Met Phe Ala Leu Ser Leu Ser Asp Asp Arg Val Ser Lys Ile Asp Arg Arg Ile Glu Ile Ile Gln Gly Leu Lys Leu Ile Pro Gly Gln Ile Lys Gln Val Leu Lys Leu Glu Pro Arg Ile Lys Lys Leu Cys Ala Thr Glu Leu Lys Asp Gln Lys Ser Leu Leu Leu Leu Gly Arg Gly Tyr Gln Phe Ala Ala Ala Leu Glu Gly Ala Leu Lys Ile Lys Glu Ile Ser Tyr Met His Ser Glu Gly Val Leu Ala Gly Glu Leu Lys His Gly Val Leu Ala Leu Val Asp Glu Asn Leu Pro Ile Ile Ala Phe Gly Thr Arg Asp Ser Leu Phe Pro Lys Val Val Ser Ser Ile Glu Gln Val Thr Ala Arg Lys Gly His Pro Ile Ile Ile Cys Asn Glu Asn Asp Glu Val Trp Ala Gln Lys Ser Lys Ser Ile Asp Leu Gln Thr Leu Glu Val Pro Gln Thr Val Asp Cys Leu Gln Gly Leu Ile Asn Ile Ile Pro Leu Gln Leu Met Ser Tyr Trp Leu Ala Val Asn Lys Gly Ile Asp Val Asp Phe Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu DNA Escherichia coli CDS atg tgt gga att gtt ggc gcg atc gcg caa cgt gat gta gca gaa atc 48 Met Cys Gly Ile Val Gly Ala Ile Ala Gln Arg Asp Val Ala Glu Ile 1 5 10 15 ctt ctt gaa ggt tta cgt cgt ctg gaa tac cgc gga tat gac tct gcc 96 Leu Leu Glu Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 20 25 30 ggt ctg gcc gtt gtt gat gca gaa ggt cat atg acc cgc ctg cgt.
- cgc 144 Gly Leu Ala Val Val Asp Ala Glu Gly His Met Thr Arg Leu Arg Arg 35 40 45 ctc ggt aaa gtc cag atg ctg gca cag gca gcg gaa gaa cat cct ctg 192 Leu Gly Lys Val Gln Met Leu Ala Gln Ala Ala Glu Glu His Pro Leu 50 55 60 cat ggc ggc act ggt att gct cac act cgc tgg gcg acc cac ggt gaa 240 His Gly Gly Thr Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Glu cct tca gaa gtg aat gcg cat ccg cat gtt tct gaa cac att gtg gtg 288 Pro Ser Glu Val Asn Ala His Pro His Val Ser Glu His Ile Val Val 85 90 95 gtg cat aac ggc atc atc gaa aac cat gaa ccg ctg cgt gaa gag cta 336 Val His Asn Gly Ile Ile Glu Asn His Glu Pro Leu Arg Glu Glu Leu aaa gcg cgt ggc tat acc ttc gtt tct gaa acc gac acc gaa gtg att 384 Lys Ala Arg Gly Tyr Thr Phe Val Ser Glu Thr Asp Thr Glu Val Ile gcc cat ctg gtg aac tgg gag ctg aaa caa ggc ggg act ctg cgt gag 432 Ala His Leu Val Asn Trp Glu Leu Lys Gln Gly Gly Thr Leu Arg Glu gcc gtt ctg cgt gct atc ccg cag ctg cgt ggt gcg tac ggt aca gtg 480 Ala Val Leu Arg Ala Ile Pro Gln Leu Arg Gly Ala Tyr Gly Thr Val atc atg gac tcc cgt cac ccg gat acc ctg ctg gcg gca cgt tct ggt 528 Ile Met Asp Ser Arg His Pro Asp Thr Leu Leu Ala Ala Arg Ser Gly agt ccg ctg gtg att ggc ctg ggg atg ggc gaa aac ttt atc gct tct 576 Ser Pro Leu Val Ile Gly Leu Gly Met Gly Glu Asn Phe Ile Ala Ser gac cag ctg gcg ctg ttg ccg gtg acc cgt cgc ttt atc ttc ctt gaa 624 Asp Gln Leu Ala Leu Leu Pro Val Thr Arg Arg Phe Ile Phe Leu Glu gag ggc gat att gcg gaa atc act cgc cgt tcg gta aac atc ttc gat 672 Glu Gly Asp Ile Ala Glu Ile Thr Arg Arg Ser Val Asn Ile Phe Asp aaa act ggc gcg gaa gta aaa cgt cag gat atc gaa tcc aat ctg caa 720 Lys Thr Gly Ala Glu Val Lys Arg Gln Asp Ile Glu Ser Asn Leu Gln tat gac gcg ggc gat aaa ggc att tac cgt cac tac atg cag aaa gag 768 Tyr Asp Ala Gly Asp Lys Gly Ile Tyr Arg His Tyr Met Gln Lys Glu atc tac gaa cag ccg aac gcg atc aaa aac acc ctt acc gga cgc atc 816 Ile Tyr Glu Gln Pro Asn Ala Ile Lys Asn Thr Leu Thr Gly Arg Ile agc cac ggt cag gtt gat tta agc gag ctg gga ccg aac gcc gac gaa 864 Ser His Gly Gln Val Asp Leu Ser Glu Leu Gly Pro Asn Ala Asp Glu ctg ctg tcg aag gtt gag cat att cag atc ctc gcc tgt ggt act tct 912 Leu Leu Ser Lys Val Glu His Ile Gln Ile Leu Ala Cys Gly Thr Ser tat aac tcc ggt atg gtt tcc cgc tac tgg ttt gaa tcg cta gca ggt 960 Tyr Asn Ser Gly Met Val Ser Arg Tyr Trp Phe Glu Ser Leu Ala Gly att ccg tgc gac gtc gaa atc gcc tct gaa ttc cgc tat cgc aaa tct 1008 Ile Pro Cys Asp Val Glu Ile Ala Ser Glu Phe Arg Tyr Arg Lys Ser gcc gtg cgt cgt aac agc ctg atg atc acc ttg tca cag tct ggc gaa 1056 Ala Val Arg Arg Asn Ser Leu Met Ile Thr Leu Ser Gln Ser Gly Glu acc gcg gat acc ctg gct ggc ctg cgt ctg tcg aaa gag ctg ggt tac 1104 Thr Ala Asp Thr Leu Ala Gly Leu Arg Leu Ser Lys Glu Leu Gly Tyr ctt ggt tca ctg gca atc tgt aac gtt ccg ggt tct tct ctg gtg cgc 1152 Leu Gly Ser Leu Ala Ile Cys Asn Val Pro Gly Ser Ser Leu Val Arg gaa tcc gat ctg gcg cta atg acc aac gcg ggt aca gaa atc ggc gtg 1200 Glu Ser Asp Leu Ala Leu Met Thr Asn Ala Gly Thr Glu Ile Gly Val gca tcc act aaa gca ttc acc act cag tta act gtg ctg ttg atg ctg 1248 Ala Ser Thr Lys Ala Phe Thr Thr Gln Leu Thr Val Leu Leu Met Leu gtg gcg aag ctg tct cgc ctg aaa ggt ctg gat gcc tcc att gaa cat 1296 Val Ala Lys Leu Ser Arg Leu Lys Gly Leu Asp Ala Ser Ile Glu His gac atc gtg cat ggt ctg cag gcg ctg ccg agc cgt att gag cag atg 1344 Asp Ile Val His Gly Leu Gln Ala Leu Pro Ser Arg Ile Glu Gln Met ctg tct cag gac aaa cgc att gaa gcg ctg gca gaa gat ttc tct gac 1392 Leu Ser Gln Asp Lys Arg Ile Glu Ala Leu Ala Glu Asp Phe Ser Asp aaa cat cac gcg ctg ttc ctg ggc cgt ggc gat cag tac cca atc gcg 1440 Lys His His Ala Leu Phe Leu Gly Arg Gly Asp Gln Tyr Pro Ile Ala ctg gaa ggc gca ttg aag ttg aaa gag atc tct tac att cac gct gaa 1488 Leu Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His Ala Glu gcc tac gct gct ggc gaa ctg aaa cac ggt ccg ctg gcg cta att gat 1536 Ala Tyr Ala Ala Gly Glu Leu Lys His Gly Pro Leu Ala Leu Ile Asp gcc gat atg ccg gtt att gtt gtt gca ccg aac aac gaa ttg ctg gaa 1584 Ala Asp Met Pro Val Ile Val Val Ala Pro.
- Asn Asn Glu Leu Leu Glu aaa ctg aaa tcc aac att gaa gaa gtt cgc gcg cgt ggc ggt cag ttg 1632 Lys Leu Lys Ser Asn Ile Glu Glu Val Arg Ala Arg Gly Gly Gln Leu tat gtc ttc gcc gat cag gat gcg ggt ttt gta agt agc gat aac atg 1680 Tyr Val Phe Ala Asp Gln Asp Ala Gly Phe Val Ser Ser Asp Asn Met cac atc atc gag atg ccg cat gtg gaa gag gtg att gca ccg atc ttc 1728 His Ile Ile Glu Met Pro His Val Glu Glu Val Ile Ala Pro Ile Phe tac acc gtt ccg ctg cag ctg ctg gct tac cat gtc gcg ctg atc aaa 1776 Tyr Thr Val Pro Leu Gln Leu Leu Ala Tyr His Val Ala Leu Ile Lys ggc acc gac gtt gac cag ccg cgt aac ctg gca aaa tcg gtt acg gtt 1824 Gly Thr Asp Val Asp Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val gag taa 1830 Glu * 10 609 PRT Escherichia coli 10 Met Cys Gly Ile Val Gly Ala Ile Ala Gln Arg Asp Val Ala Glu Ile 1 5 10 15 Leu Leu Glu Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 20 25 30 Gly Leu Ala Val Val Asp Ala Glu Gly His Met Thr Arg Leu Arg Arg 35 40 45 Leu Gly Lys Val Gln Met Leu Ala Gln Ala Ala Glu Glu His Pro Leu 50 55 60 His Gly Gly Thr Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Glu Pro Ser Glu Val Asn Ala His Pro His Val Ser Glu His Ile Val Val 85 90 95 Val His Asn Gly Ile Ile Glu Asn His Glu Pro Leu Arg Glu Glu Leu Lys Ala Arg Gly Tyr Thr Phe Val Ser Glu Thr Asp Thr Glu Val Ile Ala His Leu Val Asn Trp Glu Leu Lys Gln Gly Gly Thr Leu Arg Glu Ala Val Leu Arg Ala Ile Pro Gln Leu Arg Gly Ala Tyr Gly Thr Val Ile Met Asp Ser Arg His Pro Asp Thr Leu Leu Ala Ala Arg Ser Gly Ser Pro Leu Val Ile Gly Leu Gly Met Gly Glu Asn Phe Ile Ala Ser Asp Gln Leu Ala Leu Leu Pro Val Thr Arg Arg Phe Ile Phe Leu Glu Glu Gly Asp Ile Ala Glu Ile Thr Arg Arg Ser Val Asn Ile Phe Asp Lys Thr Gly Ala Glu Val Lys Arg Gln Asp Ile Glu Ser Asn Leu Gln Tyr Asp Ala Gly Asp Lys Gly Ile Tyr Arg His Tyr Met Gln Lys Glu Ile Tyr Glu Gln Pro Asn Ala Ile Lys Asn Thr Leu Thr Gly Arg Ile Ser His Gly Gln Val Asp Leu Ser Glu Leu Gly Pro Asn Ala Asp Glu Leu Leu Ser Lys Val Glu His Ile Gln Ile Leu Ala Cys Gly Thr Ser Tyr Asn Ser Gly Met Val Ser Arg Tyr Trp Phe Glu Ser Leu Ala Gly Ile Pro Cys Asp Val Glu Ile Ala Ser Glu Phe Arg Tyr Arg Lys Ser Ala Val Arg Arg Asn Ser Leu Met Ile Thr Leu Ser Gln Ser Gly Glu Thr Ala Asp Thr Leu Ala Gly Leu Arg Leu Ser Lys Glu Leu.
- Gly Tyr Leu Gly Ser Leu Ala Ile Cys Asn Val Pro Gly Ser Ser Leu Val Arg Glu Ser Asp Leu Ala Leu Met Thr Asn Ala Gly Thr Glu Ile Gly Val Ala Ser Thr Lys Ala Phe Thr Thr Gln Leu Thr Val Leu Leu Met Leu Val Ala Lys Leu Ser Arg Leu Lys Gly Leu Asp Ala Ser Ile Glu His Asp Ile Val His Gly Leu Gln Ala Leu Pro Ser Arg Ile Glu Gln Met Leu Ser Gln Asp Lys Arg Ile Glu Ala Leu Ala Glu Asp Phe Ser Asp Lys His His Ala Leu Phe Leu Gly Arg Gly Asp Gln Tyr Pro Ile Ala Leu Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His Ala Glu Ala Tyr Ala Ala Gly Glu Leu Lys His Gly Pro Leu Ala Leu Ile Asp Ala Asp Met Pro Val Ile Val Val Ala Pro Asn Asn Glu Leu Leu Glu Lys Leu Lys Ser Asn Ile Glu Glu Val Arg Ala Arg Gly Gly Gln Leu Tyr Val Phe Ala Asp Gln Asp Ala Gly Phe Val Ser Ser Asp Asn Met His Ile Ile Glu Met Pro His Val Glu Glu Val Ile Ala Pro Ile Phe Tyr Thr Val Pro Leu Gln Leu Leu Ala Tyr His Val Ala Leu Ile Lys Gly Thr Asp Val Asp Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu 11 1803 DNA Bacillus subtilis CDS atg tgt gga atc gta ggt tat atc ggt cag ctt gat gcg aag gaa att 48 Met Cys Gly Ile Val Gly Tyr Ile Gly Gln Leu Asp Ala Lys Glu Ile 1 5 10 15 tta tta aaa ggg tta gag aag ctt gag tat cgc ggt tat gac tct gct 96 Leu Leu Lys Gly Leu Glu Lys Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 20 25 30 ggt att gct gtt gcc aac gaa cag gga atc cat gtg ttc aaa gaa aaa.
- 144 Gly Ile Ala Val Ala Asn Glu Gln Gly Ile His Val Phe Lys Glu Lys 35 40 45 gga cgc att gca gat ctt cgt gaa gtt gtg gat gcc aat gta gaa gcg 192 Gly Arg Ile Ala Asp Leu Arg Glu Val Val Asp Ala Asn Val Glu Ala 50 55 60 aaa gcc gga att ggg cat act cgc tgg gcg aca cac ggc gaa cca agc 240 Lys Ala Gly Ile Gly His Thr Arg Trp Ala Thr His Gly Glu Pro Ser tat ctg aac gct cac ccg cat caa agc gca ctg ggc cgc ttt aca ctt 288 Tyr Leu Asn Ala His Pro His Gln Ser Ala Leu Gly Arg Phe Thr Leu 85 90 95 gtt cac aac ggc gtg atc gag aac tat gtt cag ctg aag caa gag tat 336 Val His Asn Gly Val Ile Glu Asn Tyr Val Gln Leu Lys Gln Glu Tyr ttg caa gat gta gag ctc aaa agt gac acc gat aca gaa gta gtc gtt 384 Leu Gln Asp Val Glu Leu Lys Ser Asp Thr Asp Thr Glu Val Val Val caa gta atc gag caa ttc gtc aat gga gga ctt gag aca gaa gaa gcg 432 Gln Val Ile Glu Gln Phe Val Asn Gly Gly Leu Glu Thr Glu Glu Ala ttc cgc aaa aca ctt aca ctg tta aaa ggc tct tat gca att gct tta 480 Phe Arg Lys Thr Leu Thr Leu Leu Lys Gly Ser Tyr Ala Ile Ala Leu ttc gat aac gac aac aga gaa acg att ttt gta gcg aaa aac aaa agc 528 Phe Asp Asn Asp Asn Arg Glu Thr Ile Phe Val Ala Lys Asn Lys Ser cct cta tta gta ggt ctt gga gat aca ttc aac gtc gta gca tct gat 576 Pro Leu Leu Val Gly Leu Gly Asp Thr Phe Asn Val Val Ala Ser Asp gcg atg gcg atg ctt caa gta acc aac gaa tac gta gag ctg atg gat 624 Ala Met Ala Met Leu Gln Val Thr Asn Glu Tyr Val Glu Leu Met Asp aaa gaa atg gtt atc gtc act gat gac caa gtt gtc atc aaa aac ctt 672 Lys Glu Met Val Ile Val Thr Asp Asp Gln Val Val Ile Lys Asn Leu gat ggt gac gtg att aca cgt gcg tct tat att gct gag ctt gat gcc 720 Asp Gly Asp Val Ile Thr Arg Ala Ser Tyr Ile Ala Glu Leu Asp Ala agt gat atc gaa aaa ggc acg tac cct cac tac atg ttg aaa gaa acg 768 Ser Asp Ile Glu Lys Gly Thr Tyr Pro His Tyr Met Leu Lys Glu Thr gat gag cag cct gtt gtt atg cgc aaa atc atc caa acg tat caa gat 816 Asp Glu Gln Pro Val Val Met Arg Lys Ile Ile Gln Thr Tyr Gln Asp gaa aac ggc aag ctg tct gtg cct ggc gat atc gct gcc gct gta gcg 864 Glu Asn Gly Lys Leu Ser Val Pro Gly Asp Ile Ala Ala Ala Val Ala gaa gcg gac cgc atc tat atc att ggc tgc gga aca agc tac cat gca 912 Glu Ala Asp Arg Ile Tyr Ile Ile Gly Cys Gly Thr Ser Tyr His Ala gga ctt gtc ggt aaa caa tat att gaa atg tgg gca aac gtg ccg gtt 960 Gly Leu Val Gly Lys Gln Tyr Ile Glu Met Trp Ala Asn Val Pro Val gaa gtg cat gta gcg agt gaa ttc tcc tac aac atg ccg ctt ctg tct 1008 Glu Val His Val Ala Ser Glu Phe Ser Tyr Asn Met Pro Leu Leu Ser aag aaa ccg ctc ttc att ttc ctt tct caa agc gga gaa aca gca gac 1056 Lys Lys Pro Leu Phe Ile Phe Leu Ser Gln Ser Gly Glu Thr Ala Asp agc cgc gcg gta ctc gtt caa gtc aaa gcg ctc gga cac aaa gcc ctg 1104 Ser Arg Ala Val Leu Val Gln Val Lys Ala Leu Gly His Lys Ala Leu aca atc aca aac gta cct gga tca acg ctt tct cgt gaa gct gac tat 1152 Thr Ile Thr Asn Val Pro Gly Ser Thr Leu Ser Arg Glu Ala Asp Tyr aca ttg ctg ctt cat gca ggc cct gag atc gct gtt gcg tca acg aaa 1200 Thr Leu Leu Leu His Ala Gly Pro Glu Ile Ala Val Ala Ser Thr Lys gca tac act gca caa atc gca gtt ctg gcg gtt ctt gct tct gtg gct 1248 Ala Tyr Thr Ala Gln Ile Ala Val Leu Ala Val Leu Ala Ser Val Ala gct gac aaa aat ggc atc aat atc gga ttt gac ctc gtc aaa gaa ctc 1296 Ala Asp Lys Asn Gly Ile Asn Ile Gly Phe Asp Leu Val Lys Glu Leu ggt atc gct gca aac gca atg gaa gct cta tgc gac cag aaa gac gaa 1344 Gly Ile Ala Ala Asn Ala Met Glu Ala Leu Cys Asp Gln Lys Asp Glu atg gaa atg atc gct cgt gaa tac ctg act gta tcc aga aat gct ttc 1392 Met Glu Met Ile Ala Arg Glu Tyr Leu Thr Val Ser Arg Asn Ala Phe ttc atc gga cgc ggc ctt gac tac ttc gta tgt gtc gaa ggc gca ctg 1440 Phe Ile Gly Arg Gly Leu Asp Tyr Phe Val Cys Val Glu Gly Ala Leu aag ctg aaa gag att tct tac atc cag gca gaa ggt ttt gcc ggc ggt 1488 Lys Leu Lys Glu Ile Ser Tyr Ile Gln Ala Glu Gly Phe Ala Gly Gly gag cta aag cac gga acg att gcc ttg atc gaa caa gga aca cca gta 1536 Glu Leu Lys His Gly Thr Ile Ala Leu Ile Glu Gln Gly Thr Pro Val ttc gca ctg gca act caa gag cat gta aac cta agc atc cgc gga aac 1584 Phe Ala Leu Ala Thr Gln Glu His.
- Val Asn Leu Ser Ile Arg Gly Asn gtc aaa gaa gtt gct gct cgc gga gca aac aca tgc atc atc tca ctg 1632 Val Lys Glu Val Ala Ala Arg Gly Ala Asn Thr Cys Ile Ile Ser Leu aaa ggc cta gac gat gcg gat gac aga ttc gta ttg ccg gaa gta aac 1680 Lys Gly Leu Asp Asp Ala Asp Asp Arg Phe Val Leu Pro Glu Val Asn cca gcg ctt gct ccg ttg gta tct gtt gtt cca ttg cag ctg atc gct 1728 Pro Ala Leu Ala Pro Leu Val Ser Val Val Pro Leu Gln Leu Ile Ala tac tat gct gca ctg cat cgc ggc tgt gat gtg gat aaa cct cgt aac 1776 Tyr Tyr Ala Ala Leu His Arg Gly Cys Asp Val Asp Lys Pro Arg Asn ctt gcg aag agt gtt act gtg gag taa 1803 Leu Ala Lys Ser Val Thr Val Glu PRT Bacillus subtilis 12 Met Cys Gly Ile Val Gly Tyr Ile Gly Gln Leu Asp Ala Lys Glu Ile 1 5 10 15 Leu Leu Lys Gly Leu Glu Lys Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 20 25 30 Gly Ile Ala Val Ala Asn Glu Gln Gly Ile His Val Phe Lys Glu Lys 35 40 45 Gly Arg Ile Ala Asp Leu Arg Glu Val Val Asp Ala Asn Val Glu Ala 50 55 60 Lys Ala Gly Ile Gly His Thr Arg Trp Ala Thr His Gly Glu Pro Ser Tyr Leu Asn Ala His Pro His Gln Ser Ala Leu Gly Arg Phe Thr Leu 85 90 95 Val His Asn Gly Val Ile Glu Asn Tyr Val Gln Leu Lys Gln Glu Tyr Leu Gln Asp Val Glu Leu Lys Ser Asp Thr Asp Thr Glu Val Val Val Gln Val Ile Glu Gln Phe Val Asn Gly Gly Leu Glu Thr Glu Glu Ala Phe Arg Lys Thr Leu Thr Leu Leu Lys Gly Ser Tyr Ala Ile Ala Leu Phe Asp Asn Asp Asn Arg Glu Thr Ile Phe Val Ala Lys Asn Lys Ser Pro Leu Leu Val Gly Leu Gly Asp Thr Phe Asn Val Val Ala Ser Asp Ala Met Ala Met Leu Gln Val Thr Asn Glu Tyr Val Glu Leu Met Asp Lys Glu Met Val Ile Val Thr Asp Asp Gln Val Val Ile Lys Asn Leu Asp Gly Asp Val Ile Thr Arg Ala Ser Tyr Ile Ala Glu Leu Asp Ala Ser Asp Ile Glu Lys Gly Thr Tyr Pro His Tyr Met Leu Lys Glu Thr Asp Glu Gln Pro Val Val Met Arg Lys Ile Ile Gln Thr Tyr Gln Asp Glu Asn Gly Lys Leu Ser Val Pro Gly Asp Ile Ala Ala Ala Val Ala Glu Ala Asp Arg Ile Tyr Ile Ile Gly Cys Gly Thr Ser Tyr His Ala Gly Leu Val Gly Lys Gln Tyr Ile Glu Met Trp Ala Asn Val Pro Val Glu Val His Val Ala Ser Glu Phe Ser Tyr Asn Met Pro Leu Leu Ser Lys Lys Pro Leu Phe Ile Phe Leu Ser Gln Ser Gly Glu Thr Ala Asp Ser Arg Ala Val Leu Val Gln Val Lys Ala Leu Gly His Lys Ala Leu Thr Ile Thr Asn Val Pro Gly Ser Thr Leu Ser Arg Glu Ala Asp Tyr Thr Leu Leu Leu His Ala Gly Pro Glu Ile Ala Val Ala Ser Thr Lys Ala Tyr Thr Ala Gln Ile Ala Val Leu Ala Val Leu Ala Ser Val Ala Ala Asp Lys Asn Gly Ile Asn Ile Gly Phe Asp Leu Val Lys Glu Leu Gly Ile Ala Ala Asn Ala Met Glu Ala Leu Cys Asp Gln Lys Asp Glu Met Glu Met Ile Ala Arg Glu Tyr Leu Thr Val Ser Arg Asn Ala Phe Phe Ile Gly Arg Gly Leu Asp Tyr Phe Val Cys Val Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile Gln Ala Glu Gly Phe Ala Gly Gly Glu Leu Lys His Gly Thr Ile Ala Leu Ile Glu Gln Gly Thr Pro Val Phe Ala Leu Ala Thr Gln Glu His Val Asn Leu Ser Ile Arg Gly Asn Val Lys Glu Val Ala Ala Arg Gly Ala Asn Thr Cys Ile Ile Ser Leu Lys Gly Leu Asp Asp Ala Asp Asp Arg Phe Val Leu Pro Glu Val Asn Pro Ala Leu Ala Pro Leu Val Ser Val Val Pro Leu Gln Leu Ile Ala Tyr Tyr Ala Ala Leu His Arg Gly Cys Asp Val Asp Lys Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu DNA Candida albicans CDS atg tgt ggt att ttt ggt tac gtc aat ttc ttg gtc gac aag agt aga 48 Met Cys Gly Ile Phe Gly Tyr Val Asn Phe Leu Val Asp Lys Ser Arg 1 5 10 15 ggt gaa atc att gat aat tta att gaa ggt ttg caa cga tta gaa tat 96 Gly Glu Ile Ile Asp Asn Leu Ile Glu Gly Leu Gln Arg Leu Glu Tyr 20 25 30 aga ggt tat gat tca gca ggc att gct gtt gat ggg aaa tta act aaa 144 Arg Gly Tyr Asp Ser Ala Gly Ile Ala Val Asp Gly Lys Leu Thr Lys 35 40 45 gat cct tct aat ggt gat gaa gaa tat atg gat tct att att gtt aaa 192 Asp Pro Ser Asn Gly Asp Glu Glu Tyr Met Asp Ser Ile Ile Val Lys 50 55 60 act act ggt aaa gtt aaa gtt ttg aaa caa aaa.
- atc att gat gat caa 240 Thr Thr Gly Lys Val Lys Val Leu Lys Gln Lys Ile Ile Asp Asp Gln atc gat aga tcg gcc att ttt gat aat cat gtt ggt att gct cat act 288 Ile Asp Arg Ser Ala Ile Phe Asp Asn His Val Gly Ile Ala His Thr 85 90 95 aga tgg gct aca cat ggt caa cca aaa act gaa aat tgt cat cct cat 336 Arg Trp Ala Thr His Gly Gln Pro Lys Thr Glu Asn Cys His Pro His aaa tca gat cca aag ggg gaa ttc att gtt gtt cat aat ggt att att 384 Lys Ser Asp Pro Lys Gly Glu Phe Ile Val Val His Asn Gly Ile Ile act aat tat gct gct tta aga aaa tat ctt tta tca aaa gga cat gtt 432 Thr Asn Tyr Ala Ala Leu Arg Lys Tyr Leu Leu Ser Lys Gly His Val ttt gaa agt gaa act gat act gaa tgt att gct aaa tta ttt aaa cat 480 Phe Glu Ser Glu Thr Asp Thr Glu Cys Ile Ala Lys Leu Phe Lys His ttt tat gat ttg aat gtt aaa gct ggt gtt ttc cct gat ctt aat gaa 528 Phe Tyr Asp Leu Asn Val Lys Ala Gly Val Phe Pro Asp Leu Asn Glu ttg act aaa caa gtt ttg cat gaa tta gaa ggt tct tat ggg tta tta 576 Leu Thr Lys Gln Val Leu His Glu Leu Glu Gly Ser Tyr Gly Leu Leu gtt aaa tct tat cat tat cct gga gaa gtt tgt ggt act aga aaa ggt 624 Val Lys Ser Tyr His Tyr Pro Gly Glu Val Cys Gly Thr Arg Lys Gly tct cca tta ttg gtt ggt gtt aaa act gat aag aaa tta aaa gtt gat 672 Ser Pro Leu Leu Val Gly Val Lys Thr Asp Lys Lys Leu Lys Val Asp ttt gtt gac gtt gaa ttt gaa gct caa cag caa cat cga cca caa caa 720 Phe Val Asp Val Glu Phe Glu Ala Gln Gln Gln His Arg Pro Gln Gln cca caa atc aat cat aat ggt gcc act tca gct gct gaa ttg ggc ttt 768 Pro Gln Ile Asn His Asn Gly Ala Thr Ser Ala Ala Glu Leu Gly Phe atc cca gtg gct cca ggt gaa caa aat tta aga act tct caa tca aga 816 Ile Pro Val Ala Pro Gly Glu Gln Asn Leu Arg Thr Ser Gln Ser Arg gct ttc ctt tct gaa gat gat tta cct atg cca gtt gaa ttc ttt tta 864 Ala Phe Leu Ser Glu Asp Asp Leu Pro Met Pro Val Glu Phe Phe Leu tct tct gat cct gca tca gtg gtt caa cac acc aaa aaa gtt tta ttt 912 Ser Ser Asp Pro Ala Ser Val Val Gln His Thr Lys Lys Val Leu Phe tta gaa gat gat gat att gct cat atc tat gat ggg gaa tta cgt att 960 Leu Glu Asp Asp Asp Ile Ala His Ile Tyr Asp Gly Glu Leu Arg Ile cat aga gct tcg act aaa tct gct ggg gaa tct act gtt aga cca att 1008 His Arg Ala Ser Thr Lys Ser Ala Gly Glu Ser Thr Val Arg Pro Ile caa act tta gaa atg gaa ttg aat gaa att atg aaa ggc ccc tat aaa 1056 Gln Thr Leu Glu Met Glu Leu Asn Glu Ile Met Lys Gly Pro Tyr Lys cat ttt atg caa aaa gaa att ttc gaa caa cca gat tct gct ttt aat 1104 His Phe Met Gln Lys Glu Ile Phe Glu Gln Pro Asp Ser Ala Phe Asn act atg aga ggt aga att gat ttt gaa aat tgt gtt gtt acc ctt ggt 1152 Thr Met Arg Gly Arg Ile Asp Phe Glu Asn Cys Val Val Thr Leu.
- Gly gga tta aaa tca tgg tta tct aca att aga aga tgt aga aga atc att 1200 Gly Leu Lys Ser Trp Leu Ser Thr Ile Arg Arg Cys Arg Arg Ile Ile atg att gct tgt ggt act tca tat cat tca tgt tta gcc acg aga tca 1248 Met Ile Ala Cys Gly Thr Ser Tyr His Ser Cys Leu Ala Thr Arg Ser att ttt gaa gaa ttg aca gaa atc ccc gtt tcg gtt gaa tta gct tct 1296 Ile Phe Glu Glu Leu Thr Glu Ile Pro Val Ser Val Glu Leu Ala Ser gat ttc ttg gat aga aga tct cca gtt ttc aga gat gat act tgt gta 1344 Asp Phe Leu Asp Arg Arg Ser Pro Val Phe Arg Asp Asp Thr Cys Val ttt gtt tct caa tcg ggt gaa act gcc gac tcc att ttg gct tta caa 1392 Phe Val Ser Gln Ser Gly Glu Thr Ala Asp Ser Ile Leu Ala Leu Gln tat tgt ttg gaa aga gga gct tta act gtt ggt atc gtt aac tct gtt 1440 Tyr Cys Leu Glu Arg Gly Ala Leu Thr Val Gly Ile Val Asn Ser Val ggt tct tca atg tct aga caa acc cat tgt ggg gtt cat att aat gct 1488 Gly Ser Ser Met Ser Arg Gln Thr His Cys Gly Val His Ile Asn Ala ggg cca gaa att ggt gtt gcc tca act aaa gct tac aca tct caa tat 1536 Gly Pro Glu Ile Gly Val Ala Ser Thr Lys Ala Tyr Thr Ser Gln Tyr att gcc ttg gtg atg ttt gcc ctt tct tta tct aat gat tct att tcc 1584 Ile Ala Leu Val Met Phe Ala Leu Ser Leu Ser Asn Asp Ser Ile Ser aga aag gga aga cat gaa gaa att att aaa ggt tta caa aaa atc cct 1632 Arg Lys Gly Arg His Glu Glu Ile Ile Lys Gly Leu Gln Lys Ile Pro 530 535.
- 540 gaa caa att aaa caa gtt ttg aaa tta gaa aac aag atc aaa gat tta 1680 Glu Gln Ile Lys Gln Val Leu Lys Leu Glu Asn Lys Ile Lys Asp Leu tgt aat agt tca ttg aat gat caa aaa tct tta tta tta tta ggt aga 1728 Cys Asn Ser Ser Leu Asn Asp Gln Lys Ser Leu Leu Leu Leu Gly Arg ggt tat caa ttt gct act gct tta gaa ggg gct tta aaa att aaa gaa 1776 Gly Tyr Gln Phe Ala Thr Ala Leu Glu Gly Ala Leu Lys Ile Lys Glu att tct tat atg cat tct gaa ggg gta tta gct ggt gaa tta aaa cat 1824 Ile Ser Tyr Met His Ser Glu Gly Val Leu Ala Gly Glu Leu Lys His ggt ata tta gca tta gtc gat gaa gat tta cca att att gcc ttt gcc 1872 Gly Ile Leu Ala Leu Val Asp Glu Asp Leu Pro Ile Ile Ala Phe Ala act aga gat tca tta ttt cct aaa gtt atg tcc gct att gaa caa gtc 1920 Thr Arg Asp Ser Leu Phe Pro Lys Val Met Ser Ala Ile Glu Gln Val act gct aga gat ggt aga cca att gtt att tgt aat gaa ggt gat gct 1968 Thr Ala Arg Asp Gly Arg Pro Ile Val Ile Cys Asn Glu Gly Asp Ala att att tct aat gat aaa gtt cat act act tta gaa gtt cca gaa acc 2016 Ile Ile Ser Asn Asp Lys Val His Thr Thr Leu Glu Val Pro Glu Thr gtt gat tgt tta caa ggg tta tta aat gtt att cca tta caa ttg att 2064 Val Asp Cys Leu Gln Gly Leu Leu Asn Val Ile Pro Leu Gln Leu Ile agt tat tgg ttg gct gtg aat aga ggt att gat gtt gat ttc cct cgt 2112 Ser Tyr Trp Leu Ala Val Asn Arg Gly Ile Asp Val Asp Phe Pro Arg aac ttg gct aaa tca gtt act gtt gag taa 2142 Asn Leu Ala Lys Ser Val Thr Val Glu PRT Candida albicans 14 Met Cys Gly Ile Phe Gly Tyr Val Asn Phe Leu Val Asp Lys Ser Arg 1 5 10 15 Gly Glu Ile Ile Asp Asn Leu Ile Glu Gly Leu Gln Arg Leu Glu Tyr 20 25 30 Arg Gly Tyr Asp Ser Ala Gly Ile Ala Val Asp Gly Lys Leu Thr Lys 35 40 45 Asp Pro Ser Asn Gly Asp Glu Glu Tyr Met Asp Ser Ile Ile Val Lys 50 55 60 Thr Thr Gly Lys Val Lys Val Leu Lys Gln Lys Ile Ile Asp Asp Gln Ile Asp Arg Ser Ala Ile Phe Asp Asn His Val Gly Ile Ala His Thr 85 90 95 Arg Trp Ala Thr His Gly Gln Pro Lys Thr Glu Asn Cys His Pro His Lys Ser Asp Pro Lys Gly Glu Phe Ile Val Val His Asn Gly Ile Ile Thr Asn Tyr Ala Ala Leu Arg Lys Tyr Leu Leu Ser Lys Gly His Val Phe Glu Ser Glu Thr Asp Thr Glu Cys Ile Ala Lys Leu Phe Lys His Phe Tyr Asp Leu Asn Val Lys Ala Gly Val Phe Pro Asp Leu Asn Glu Leu Thr Lys Gln Val Leu His Glu Leu Glu Gly Ser Tyr Gly Leu Leu Val Lys Ser Tyr His Tyr Pro Gly Glu Val Cys Gly Thr Arg Lys Gly Ser Pro Leu Leu Val Gly Val Lys Thr Asp Lys Lys Leu Lys Val Asp Phe Val Asp Val Glu Phe Glu Ala Gln Gln Gln His Arg Pro Gln Gln Pro Gln Ile Asn His Asn Gly Ala Thr Ser Ala Ala Glu Leu Gly Phe Ile Pro Val Ala Pro Gly Glu Gln Asn Leu Arg Thr Ser Gln Ser Arg Ala Phe Leu Ser Glu Asp Asp Leu Pro Met Pro Val Glu Phe Phe Leu Ser Ser Asp Pro Ala Ser Val Val Gln His Thr Lys Lys Val Leu Phe Leu Glu Asp Asp Asp Ile Ala His Ile Tyr Asp Gly Glu Leu Arg Ile His Arg Ala Ser Thr Lys Ser Ala Gly Glu Ser Thr Val Arg Pro Ile Gln Thr Leu Glu Met Glu Leu Asn Glu Ile Met Lys Gly Pro Tyr Lys His Phe Met Gln Lys Glu Ile Phe Glu Gln Pro Asp Ser Ala Phe Asn Thr Met Arg Gly Arg Ile Asp Phe Glu Asn Cys Val Val Thr Leu Gly Gly Leu Lys Ser Trp Leu Ser Thr Ile Arg Arg Cys Arg Arg Ile Ile Met Ile Ala Cys Gly Thr Ser Tyr His Ser Cys Leu Ala Thr Arg Ser Ile Phe Glu Glu Leu Thr Glu Ile Pro Val Ser Val Glu Leu Ala Ser Asp Phe Leu Asp Arg Arg Ser Pro Val Phe Arg Asp Asp Thr Cys Val Phe Val Ser Gln Ser Gly Glu Thr Ala Asp Ser Ile Leu Ala Leu Gln Tyr Cys Leu Glu Arg Gly Ala Leu Thr Val Gly Ile Val Asn Ser Val Gly Ser Ser Met Ser Arg Gln Thr His Cys Gly Val His Ile Asn Ala Gly Pro Glu Ile Gly Val Ala Ser Thr Lys Ala Tyr Thr Ser Gln Tyr Ile Ala Leu Val Met Phe Ala Leu Ser Leu Ser Asn Asp Ser Ile Ser Arg Lys Gly Arg His Glu Glu Ile Ile Lys Gly Leu Gln Lys Ile Pro Glu Gln Ile Lys Gln Val Leu Lys Leu Glu Asn Lys Ile Lys Asp Leu Cys Asn Ser Ser Leu Asn Asp Gln.
- Lys Ser Leu Leu Leu Leu Gly Arg Gly Tyr Gln Phe Ala Thr Ala Leu Glu Gly Ala Leu Lys Ile Lys Glu Ile Ser Tyr Met His Ser Glu Gly Val Leu Ala Gly Glu Leu Lys His Gly Ile Leu Ala Leu Val Asp Glu Asp Leu Pro Ile Ile Ala Phe Ala Thr Arg Asp Ser Leu Phe Pro Lys Val Met Ser Ala Ile Glu Gln Val Thr Ala Arg Asp Gly Arg Pro Ile Val Ile Cys Asn Glu Gly Asp Ala Ile Ile Ser Asn Asp Lys Val His Thr Thr Leu Glu Val Pro Glu Thr Val Asp Cys Leu Gln Gly Leu Leu Asn Val Ile Pro Leu Gln Leu Ile Ser Tyr Trp Leu Ala Val Asn Arg Gly Ile Asp Val Asp Phe Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu PRT Saccharomyces cerevisiae 15 Met Ala Ser Ser Pro Gln Val His Pro Tyr Lys Lys His Leu Met Gln 1 5 10 15 Ser Gln His Ile Asn Phe Asp Asn Arg Gly Leu Gln Phe Gln Asn Ser 20 25 30 Ser Leu Lys Val Gly Gln Asp Phe Ser Asp Asn Lys Glu Asn Arg Glu 35 40 45 Asn Arg Asp Asn Glu Asp Phe Ser Thr Ala Asp Leu Pro Lys Arg Ser 50 55 60 Ala Asn Gln Pro Leu Ile Asn Glu His Leu Arg Ala Ala Ser Val Pro Leu Leu Ser Asn Asp Ile Gly Asn Ser Gln Glu Glu Asp Phe Val Pro 85 90 95 Val Pro Pro Pro Gln Leu His Leu Asn Asn Ser Asn Asn Thr Ser Leu Ser Ser Leu Gly Ser Thr Pro Thr Asn Ser Pro Ser Pro Gly Ala Leu Arg Gln Thr Asn Ser Ser Thr Ser Leu Thr Lys Glu Gln Ile Lys Lys Arg Thr Arg Ser Val Asp Leu Ser His Met Tyr Leu Leu Asn Gly Ser Ser Asp Thr Gln Leu Thr Ala Thr Asn Glu Ser Val Ala Asp Leu Ser His Gln Met Ile Ser Arg Tyr Leu Gly Gly Lys Asn Asn Thr Ser Leu Val Pro Arg Leu Lys Thr Ile Glu Met Tyr Arg Gln Asn Val Lys Lys Ser Lys Asp Pro Glu Val Leu Phe Gln Tyr Ala Gln Tyr Met Leu Gln Thr Ala Leu Thr Ile Glu Ser Ser Asn Ala Leu Val Gln Asp Ser Asp Lys Glu Gly Asn Val Ser Gln Ser Asp Leu Lys Leu Gln Phe Leu Lys Glu Ala Gln Ser Tyr Leu Lys Lys Leu Ser Ile Lys Gly Tyr Ser Asp Ala Gln Tyr Leu Leu Ala Asp Gly Tyr Ser Ser Gly Ala Phe Gly Lys Ile Glu Asn Lys Glu Ala Phe Val Leu Phe Gln Ala Ala Ala Lys His Gly His Ile Glu Ser Ala Tyr Arg Ala Ser His Cys Leu Glu Glu Gly Leu Gly Thr Thr Arg Asp Ser Arg Lys Ser Val Asn Phe Leu Lys Phe Ala Ala Ser Arg Asn His Pro Ser Ala Met Tyr Lys Leu Gly Leu Tyr Ser Phe Tyr Gly Arg Met Gly Leu Pro Thr Asp Val Asn Thr Lys Leu Asn Gly Val Lys Trp Leu Ser Arg Ala Ala Ala Arg Ala Asn Glu Leu Thr Ala Ala Ala Pro Tyr Glu Leu Ala Lys Ile Tyr His Glu Gly Phe Leu Asp Val Val Ile Pro Asp Glu Lys Tyr Ala Met Glu Leu Tyr Ile Gln Ala Ala Ser Leu Gly His Val Pro Ser Ala Thr Leu Leu Ala Gln Ile Tyr Glu Thr Gly Asn Asp Thr Val Gly Gln Asp Thr Ser Leu Ser Val His Tyr Tyr Thr Gln Ala Ala Leu Lys Gly Asp Ser Val Ala Met Leu Gly Leu Cys Ala Trp Tyr Leu Leu Gly Ala Glu Pro Ala Phe Glu Lys Asp Glu Asn Glu Ala Phe Gln Trp Ala Leu Arg Ala Ala Asn Ala Gly Leu Pro Lys Ala Gln Phe Thr Leu Gly Tyr Phe Tyr Glu His Gly Lys Gly Cys Asp Arg Asn Met Glu Tyr Ala Trp Lys Trp Tyr Glu Lys Ala Ala Gly Asn Glu Asp Lys Arg Ala Ile Asn Lys Leu Arg Ser Arg Asp Gly Gly Leu Ala Ser Ile Gly Lys Lys Gln His Lys Lys Asn Lys Ser Ile Ser Thr Leu Asn Leu Phe Ser Thr Val Asp Ser Gln Thr Ser Asn Val Gly Ser Asn Ser Arg Val Ser Ser Lys Ser Glu Thr Phe Phe Thr Gly Asn Pro Lys Arg Asp Arg Glu Pro Gln Gly Leu Gln Ile Asn Met Asn Ser Asn Thr Asn Arg Asn Gly Ile Lys Thr Gly Ser Asp Thr Ser Ile Arg Lys Ser Ser Ser Ser Ala Lys Gly Met Ser Arg Glu Val Ala Glu Gln Ser Met Ala Ala Lys Gln Glu Val Ser Leu Ser Asn Met Gly Ser Ser Asn Met Ile Arg Lys Asp Phe Pro Ala Val Lys Thr Glu Ser Lys Lys Pro Thr Ser Leu Lys Asn Lys Lys Asp Lys Gln Gly Lys Lys Lys Lys Asp Cys Val Ile Met PRT Saccharomyces cerevisiae 16 Met Ser Ser Val Asp Val Leu Leu Thr Val Gly Lys Leu Asp Ala Ser.
- 1 5 10 15 Leu Ala Leu Leu Thr Thr Gln Asp His His Val Ile Glu Phe Pro Thr 20 25 30 Val Leu Leu Pro Glu Asn Val Lys Ala Gly Ser Ile Ile Lys Met Gln 35 40 45 Val Ser Gln Asn Leu Glu Glu Glu Lys Lys Gln Arg Asn His Phe Lys 50 55 60 Ser Ile Gln Ala Lys Ile Leu Glu Lys Tyr Gly Thr His Lys Pro Glu Ser Pro Val Leu Lys Ile Val Asn Val Thr Gln Thr Ser Cys Val Leu 85 90 95 Ala Trp Asp Pro Leu Lys Leu Gly Ser Ala Lys Leu Lys Ser Leu Ile Leu Tyr Arg Lys Gly Ile Arg Ser Met Val Ile Pro Asn Pro Phe Lys Val Thr Thr Thr Lys Ile Ser Gly Leu Ser Val Asp Thr Pro Tyr Glu Phe Gln Leu Lys Leu Ile Thr Thr Ser Gly Thr Leu Trp Ser Glu Lys Val Ile Leu Arg Thr His Lys Met Thr Asp Met Ser Gly Ile Thr Val Cys Leu Gly Pro Leu Asp Pro Leu Lys Glu Ile Ser Asp Leu Gln Ile Ser Gln Cys Leu Ser His Ile Gly Ala Arg Pro Leu Gln Arg His Val Ala Ile Asp Thr Thr His Phe Val Cys Asn Asp Leu Asp Asn Glu Glu Ser Asn Glu Glu Leu Ile Arg Ala Lys His Asn Asn Ile Pro Ile Val Arg Pro Glu Trp Val Arg Ala Cys Glu Val Glu Lys Arg Ile Val Gly Val Arg Gly Phe Tyr Leu Asp Ala Asp Gln Ser Ile Leu Lys Asn Tyr Thr Phe Pro Pro Val Asn Glu Glu Glu Leu Ser Tyr Ser Lys Glu Asn Glu Pro Val Ala Glu Val Ala Asp Glu Asn Lys Met Pro Glu Asp Thr Thr Asp Val Glu Gln Val Ala Ser Pro Asn Asp Asn Glu Ser Asn Pro Ser Glu Ala Lys Glu Gln Gly Glu Lys Ser Gly His Glu Thr Ala Pro Val Ser Pro Val Glu Asp Pro Leu His Ala Ser Thr Ala Leu Glu Asn Glu Thr Thr Ile Glu Thr Val Asn Pro Ser Val Arg Ser Leu Lys Ser Glu Pro Val Gly Thr Pro Asn Ile Glu Glu Asn Lys Ala Asp Ser Ser Ala Glu Ala Val Val Glu Glu Pro Asn Glu Ala Val Ala Glu Ser Ser Pro Asn Glu Glu Ala Thr Gly Gln Lys Ser Glu Asp Thr Asp Thr His Ser Asn Glu Gln Ala Asp Asn Gly Phe Val Gln Thr Glu Glu Val Ala Glu Asn Asn Ile Thr Thr Glu Ser Ala Gly Glu Asn Asn Glu Pro Ala Asp Asp Ala Ala Met Glu Phe Gly Arg Pro Glu Ala Glu Ile Glu Thr Pro Glu Val Asn Glu Ser Ile Glu Asp Ala Asn Glu Pro Ala Glu Asp Ser Asn Glu Pro Val Glu Asp Ser Asn Lys.
- Asn Pro Glu Leu Lys Pro Pro Gly Leu Val Glu Cys Pro Phe Tyr Val Gly Lys Asp Thr Ile Ser Ser Cys Lys Lys Ile Ile Glu Leu Leu Cys Arg Phe Leu Pro Arg Ser Leu Asn Cys Gly Trp Asp Ser Thr Lys Ser Met Gln Ala Thr Ile Val Asn Asn Tyr Leu Met Tyr Ser Leu Lys Ser Phe Ile Ala Ile Thr Pro Ser Leu Val Asp Phe Thr Ile Asp Tyr Leu Lys Gly Leu Thr Lys Lys Asp Pro Ile His Asp Ile Tyr Tyr Lys Thr Ala Met Ile Thr Ile Leu Asp His Ile Glu Thr Lys Glu Leu Asp Met Ile Thr Ile Leu Asn Glu Thr Leu Asp Pro Leu Leu Ser Leu Leu Asn Asp Leu Pro Pro Arg Asp Ala Asp Ser Ala Arg Leu Met Asn Cys Met Ser Asp Leu Leu Asn Ile Gln Thr Asn Phe Leu Leu Asn Arg Gly Asp Tyr Glu Leu Ala Leu Gly Val Ser Asn Thr Ser Thr Glu Leu Ala Leu Asp Ser Phe Glu Ser Trp Tyr Asn Leu Ala Arg Cys His Ile Lys Lys Glu Glu Tyr Glu Lys Ala Leu Phe Ala Ile Asn Ser Met Pro Arg Leu Arg Lys Asn Asp Gly His Leu Glu Thr Met Tyr Ser Arg Phe Leu Thr Ser Asn Tyr Tyr Lys Lys Pro Leu Asn Gly Thr Arg Glu His Tyr Asp Leu Thr Ala Met Glu Phe Thr Asn Leu Ser Gly Thr Leu Arg Asn Trp Lys Glu Asp Glu Leu Lys Arg Gln Ile Phe Gly Arg Ile Ala Met Ile Asn Glu Lys Lys Ile Gly Tyr Thr Lys Glu Ile Trp Asp Asp Ile Ala Ile Lys Leu Gly Pro Ile Cys Gly Pro Gln Ser Val Asn Leu Ile Asn Tyr Val Ser Pro Gln Glu Val Lys Asn Ile Lys Asn Ile Asn Leu Ile Ala Arg Asn Thr Ile Gly Lys Gln Leu Gly Trp Phe Ser Gly Lys Ile Tyr Gly Leu Leu Met Glu Ile Val Asn Lys Ile Gly Trp Asn Gly Leu Leu Asn Ile Arg Thr Glu Ala Phe Met Met Glu Thr Glu Phe Tyr Gln Ala Ser Asn Asn Ile Ile Asp Glu Asn Gly His Ile Pro Met Glu Ser Arg Lys Lys Arg Phe Cys Glu Gly Trp Leu Asp Asp Leu Phe Leu Asp Leu Tyr Gln Asp Leu Lys Leu Ser Lys Ile Ser Leu Ser Asn Lys Asp Glu Lys His Ser Gly Leu Glu Trp Glu Leu Leu Gly Leu Ile Met Leu Arg Thr Trp His Trp Glu Asp Ala Val Ala Cys Leu Arg Thr Ser Ile Val Ala Arg Phe Asp Pro Val Ser Cys Gln Gln Leu Leu Lys Ile Tyr Leu Gln Pro Pro Lys Asn Ile Gln Glu Val Thr Leu Leu Asp Thr Asp Thr Ile Ile Ser Leu Leu Ile Lys Lys Ile Ser Tyr Asp Cys Arg Tyr Tyr Asn Tyr Cys Gln Ile Phe Asn Leu Gln Leu Leu Glu Lys Leu Cys Asn Glu Leu Gly Thr His Ile Leu Arg Asn Lys Ile Leu Leu Gln Pro Ser Ile Gly Asp Glu Ile Met Val Met Ile Asp Ala Met Leu Ala Trp Ile Ala Asp Leu Asp His Thr Val Gln Pro PRT Saccharomyces cerevisiae 18 Met Ala Phe Ser Asp Phe Ala Ala Ile Cys Ser Lys Thr Pro Leu Pro 1 5 10 15 Leu Cys Ser Val Ile Lys Ser Lys Thr His Leu Ile Leu Ser Asn Ser 20 25 30 Thr Ile Ile His Asp Phe Asp Pro Leu Asn Leu Asn Val Gly Val Leu 35 40 45 Pro Arg Cys Tyr Ala Arg Ser Ile Asp Leu Ala Asn Thr Val Ile Phe 50 55 60 Asp Val Gly Asn Ala Phe Ile Asn Ile Gly Ala Leu Gly Val Ile Leu Ile Ile Leu Tyr Asn Ile Arg Gln Lys Tyr Thr Ala Ile Gly Arg Ser 85 90 95 Glu Tyr Leu Tyr Phe Phe Gln Leu Thr Leu Leu Leu Ile Ile Phe Thr Leu Val Val Asp Cys Gly Val Ser Pro Pro Gly Ser Gly Se.
- Tyr Pro Tyr Phe Val Ala Ile Gln Ile Gly Leu Ala Gly Ala Cys Cys Trp Ala Leu Leu Ile Ile Gly Phe Leu Gly Phe Asn Leu Trp Glu Asp Gly Thr Thr Lys Ser Met Leu Leu Val Arg Gly Thr Ser Met Leu Gly Phe Ile Ala Asn Phe Leu Ala Ser Ile Leu Thr Phe Lys Ala Trp Ile Thr Asp His Lys Val Ala Thr Met Asn Ala Ser Gly Met Ile Val Val Val Tyr Ile Ile Asn Ala Ile Phe Leu Phe Val Phe Val Ile Cys Gln Leu Leu Val Ser Leu Leu Val Val Arg Asn Leu Trp Val Thr Gly Ala Ile Phe Leu Gly Leu Phe Phe Phe Val Ala Gly Gln Val Leu Val Tyr Ala Phe Ser Thr Gln Ile Cys Glu Gly Phe Lys His Tyr Leu Asp Gly Leu Phe Phe Gly Ser Ile Cys Asn Val Phe Thr Leu Met Met Val Tyr Lys Thr Trp Asp Met Thr Thr Asp Asp.
- Asp Leu Glu Phe Gly Val Ser Val Ser Lys Asp Gly Asp Val Val Tyr Asp Asn Gly Phe Met DNA Aspergillus niger CDS misc_feature n = A,T,C or G 19 gat ttc ctc ttc gct cgg acg atg atc ggt gtg ttc aag aac att gag 48 Asp Phe Leu Phe Ala Arg Thr Met Ile Gly Val Phe Lys Asn Ile Glu 1 5 10 15 tac atg tgc tcc agg aca tca agc aag acg tgg ggc aaa gat gcc tgg 96 Tyr Met Cys Ser Arg Thr Ser Ser Lys Thr Trp Gly Lys Asp Ala Trp 20 25 30 aag aag att gtg gtt tgt gtc atc agt gac ggt cgt gcg aag atc aat 144 Lys Lys Ile Val Val Cys Val Ile Ser Asp Gly Arg Ala Lys Ile Asn 35 40 45 ccg cga acg cgc gct gtc ctt gct ggt ttg gga tgc tat cag gat gga 192 Pro Arg Thr Arg Ala Val Leu Ala Gly Leu Gly Cys Tyr Gln Asp Gly 50 55 60 att gcc aag cag caa gtt aac ggc aag gat gtc aca gca cac atc tac 240 Ile Ala Lys Gln Gln Val Asn Gly Lys Asp Val Thr Ala His Ile Tyr gaa tat acc acg cag gtg ggt cta gaa ttg aag gga ggc caa gtt agc 288 Glu Tyr Thr Thr Gln Val Gly Leu Glu Leu Lys Gly Gly Gln Val Ser 85 90 95 ctc aag cct cga act gga tgc ccc gtt cag atg atc ttt tgt ctg aag 336 Leu Lys Pro Arg Thr Gly Cys Pro Val Gln Met Ile Phe Cys Leu Lys gaa aag aac cag aag aag atc aac tcg cac cgc tgg ttc ttc cag gca 384 Glu Lys Asn Gln Lys Lys Ile Asn Ser His Arg Trp Phe Phe Gln Ala ttt ggt cgc gtg ctg gat ccc aat atc tgc gtt ctg ttg gac gcg ggt 432 Phe Gly Arg Val Leu Asp Pro Asn Ile Cys Val Leu Leu Asp Ala Gly acc cgg ccn gga aag gac tcg atc tac cat ctt tgg aag gcn ttc gat 480 Thr Arg Pro Gly Lys Asp Ser Ile Tyr His Leu Trp Lys Ala Phe Asp gtt gac ccg atg tgt gga ggt gct tgt ggt gag atc aag gtc atg tng 528 Val Asp Pro Met Cys Gly Gly Ala Cys Gly Glu Ile Lys Val Met Xaa tcg cat gga aag aag ctg ctc aac ccg ttg gtt gcc ggg 567 Ser His Gly Lys Lys Leu Leu Asn Pro Leu Val Ala Gly PRT Aspergillus niger VARIANT 176 Xaa = Any Amino Acid 20 Asp Phe Leu Phe Ala Arg Thr Met Ile Gly Val Phe Lys Asn Ile Glu 1 5 10 15 Tyr Met Cys Ser Arg Thr Ser Ser Lys Thr Trp Gly Lys Asp Ala Trp 20 25 30 Lys Lys Ile Val Val Cys Val Ile Ser Asp Gly Arg Ala Lys Ile Asn 35 40 45 Pro Arg Thr Arg Ala Val Leu Ala Gly Leu Gly Cys Tyr Gln Asp Gly 50 55 60 Ile Ala Lys Gln Gln Val Asn Gly Lys Asp Val Thr Ala His Ile Tyr Glu Tyr Thr Thr Gln Val Gly Leu Glu Leu Lys Gly Gly Gln Val Ser 85 90 95 Leu Lys Pro Arg Thr Gly Cys Pro Val Gln Met Ile Phe Cys Leu Lys Glu Lys Asn Gln Lys Lys Ile Asn Ser His Arg Trp Phe Phe Gln Ala Phe Gly Arg Val Leu Asp Pro Asn Ile Cys Val Leu Leu Asp Ala Gly Thr Arg Pro Gly Lys Asp Ser Ile Tyr His Leu Trp Lys Ala Phe Asp Val Asp Pro Met Cys Gly Gly Ala Cys Gly Glu Ile Lys Val Met Xaa Ser His Gly Lys Lys Leu Leu Asn Pro Leu Val Ala Gly DNA Aspergillus niger CDS (1)....
- (567) 21 gag atc ggc ttc act cgg act ctg cat ggc gtg atg cag aat atc acc 48 Glu Ile Gly Phe Thr Arg Thr Leu His Gly Val Met Gln Asn Ile Thr 1 5 10 15 cac ctt tgc tcg cga tcc aag tcc cgt acc tgg ggt aag gat ggt tgg 96 His Leu Cys Ser Arg Ser Lys Ser Arg Thr Trp Gly Lys Asp Gly Trp 20 25 30 aag aag att gtg gtc tgc atc atc gcc gac ggt cgt aag aag gtg cac 144 Lys Lys Ile Val Val Cys Ile Ile Ala Asp Gly Arg Lys Lys Val His 35 40 45 ccg cgg acc ctc aat gct ttg gcg gct ttg ggt gtc tac cag gaa ggt 192 Pro Arg Thr Leu Asn Ala Leu Ala Ala Leu Gly Val Tyr Gln Glu Gly 50 55 60 atc gcc aag aac gtt gtc aac cag aag cag gtc aac gcg cac gtc tac 240 Ile Ala Lys Asn Val Val Asn Gln Lys Gln Val Asn Ala His Val Tyr gaa tac acg acg cag gta tcg ctc gat ccc gac ttg aag ttc aag ggc 288 Glu Tyr Thr Thr Gln Val Ser Leu Asp Pro Asp Leu Lys Phe Lys Gly 85 90 95 gct gag aag ggc atc atg ccg tgc cag gtg ctc ttc tgt ttg aaa gaa 336 Ala Glu Lys Gly Ile Met Pro Cys Gln Val Leu Phe Cys Leu Lys Glu cac aac aag aag aaa ttg aac tca cac cgt tgg ttc ttc aac gct ttt 384 His Asn Lys Lys Lys Leu Asn Ser His Arg Trp Phe Phe Asn Ala Phe ggc cga gca cta cag ccg aac att tgc atc ctg ctc gat gtg ggt acc 432 Gly Arg Ala Leu Gln Pro Asn Ile Cys Ile Leu Leu Asp Val Gly Thr aaa cct gcg cct acc gca ctc tac cac ctc tgg aaa gcg ttc gac cag 480 Lys Pro Ala Pro Thr Ala Leu Tyr His Leu Trp Lys Ala Phe Asp Gln 145 150.
- 155 160 aac tcg aac gtc gcg gga gcg gct ggt gaa atc aag gcg ggc aaa ggc 528 Asn Ser Asn Val Ala Gly Ala Ala Gly Glu Ile Lys Ala Gly Lys Gly aag ggt atg ctc ggt ctt ctg aac cct ctg gtt gcc agt 567 Lys Gly Met Leu Gly Leu Leu Asn Pro Leu Val Ala Ser PRT Aspergillus niger 22 Glu Ile Gly Phe Thr Arg Thr Leu His Gly Val Met Gln Asn Ile Thr 1 5 10 15 His Leu Cys Ser Arg Ser Lys Ser Arg Thr Trp Gly Lys Asp Gly Trp 20 25 30 Lys Lys Ile Val Val Cys Ile Ile Ala Asp Gly Arg Lys Lys Val His 35 40 45 Pro Arg Thr Leu Asn Ala Leu Ala Ala Leu Gly Val Tyr Gln Glu Gly 50 55 60 Ile Ala Lys Asn Val Val Asn Gln Lys Gln Val Asn Ala His Val Tyr Glu Tyr Thr Thr Gln Val Ser Leu Asp Pro Asp Leu Lys Phe Lys Gly 85 90 95 Ala Glu Lys Gly Ile Met Pro Cys Gln Val Leu Phe Cys Leu Lys Glu His Asn Lys Lys Lys Leu Asn Ser His Arg Trp Phe Phe Asn Ala Phe Gly Arg Ala Leu Gln Pro Asn Ile Cys Ile Leu Leu Asp Val Gly Thr Lys Pro Ala Pro Thr Ala Leu Tyr His Leu Trp Lys Ala Phe Asp Gln Asn Ser Asn Val Ala Gly Ala Ala Gly Glu Ile Lys Ala Gly Lys Gly Lys Gly Met Leu Gly Leu Leu Asn Pro Leu Val Ala Ser DNA Aspergillus fumigatus CDS atg ggc act cca agg ccc tat tcg gcg cat tca ccg cag gaa agc cga 48 Met Gly Thr Pro Arg Pro Tyr Ser Ala His Ser Pro Gln Glu Ser Arg 1 5 10 15 agc agc ttc tac tcg cag ccc tcc caa tct cct aca cag cct acc tac 96 Ser Ser Phe Tyr Ser Gln Pro Ser Gln Ser Pro Thr Gln Pro Thr Tyr 20 25 30 ggt cgc gat gac gcg gaa gat caa caa caa tcc ctc ctt cga cgc agt 144 Gly Arg Asp Asp Ala Glu Asp Gln Gln Gln Ser Leu Leu Arg Arg Ser 35 40 45 tta gcc agt ccc aat ggc tgg tca tac gat gat ccc aac gta tct acc 192 Leu Ala Ser Pro Asn Gly Trp Ser Tyr Asp Asp Pro Asn Val Ser Thr 50 55 60 gac tct ctg agg cga tac aca ttg cat gat ccg ggg ata acc gca ttt 240 Asp Ser Leu Arg Arg Tyr Thr Leu His Asp Pro Gly Ile Thr Ala Phe gct ccc ccg tac ccg gag tcc gag gcc gcc gat gta cgc agc gca cgg 288 Ala Pro Pro Tyr Pro Glu Ser Glu Ala Ala Asp Val Arg Ser Ala Arg 85 90 95 atg tcg gga tac agt gga atc gag atg gat gcc tgg caa aga cga caa.
- 336 Met Ser Gly Tyr Ser Gly Ile Glu Met Asp Ala Trp Gln Arg Arg Gln ggg gtg aaa cca agt gcg cta cgg cga tat gga acc agg aag atc aac 384 Gly Val Lys Pro Ser Ala Leu Arg Arg Tyr Gly Thr Arg Lys Ile Asn ctc gtc cag ggt tcg gta ctc agc gtt gac tac ccg gtg ccg agt gcg 432 Leu Val Gln Gly Ser Val Leu Ser Val Asp Tyr Pro Val Pro Ser Ala att cag aac gcg atc cag gcg gag tat cgg gat gcg gag gaa gcg ttt 480 Ile Gln Asn Ala Ile Gln Ala Glu Tyr Arg Asp Ala Glu Glu Ala Phe cat gaa gaa ttc acg cat atg cgg tat act gcc gcc acc tgc gac cca 528 His Glu Glu Phe Thr His Met Arg Tyr Thr Ala Ala Thr Cys Asp Pro gat gaa ttc act ctg cgc aac ggc tac aac cta cgt ccc gct atg tat 576 Asp Glu Phe Thr Leu Arg Asn Gly Tyr Asn Leu Arg Pro Ala Met Tyr aac cgt cat acg gaa cgt ctc atc gcg atc act tat tat aat gag gat 624 Asn Arg His Thr Glu Arg Leu Ile Ala Ile Thr Tyr Tyr Asn Glu Asp aaa gtt ctg acc gcc cga act ctg cat gga gtg atg caa aac gtc cgg 672 Lys Val Leu Thr Ala Arg Thr Leu His Gly Val Met Gln Asn Val Arg gac att gtg aac ctg aag aag tca gaa ttt tgg aac aag gga gga ccg 720 Asp Ile Val Asn Leu Lys Lys Ser Glu Phe Trp Asn Lys Gly Gly Pro gca tgg cag aag att gtg gtg tgt ctc gtg ttt gat gga att gag cct 768 Ala Trp Gln Lys Ile Val Val Cys Leu Val Phe Asp Gly Ile Glu Pro tgc gat aag aat act ctg gat gtc ctc gcc acg ata ggt gtg tat cag 816 Cys Asp Lys Asn Thr Leu Asp Val Leu Ala Thr Ile Gly Val Tyr Gln gat ggg gtg atg aaa aaa gat gtg gac ggg cgc gag aca gtc gca cat 864 Asp Gly Val Met Lys Lys Asp Val Asp Gly Arg Glu Thr Val Ala His att ttc gag tat acg aca caa tta tcc gtc acc ccg aca cag cag ctg 912 Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val Thr Pro Thr Gln Gln Leu gtc aga ccg cag cct aat gat cct agc aat ctc ccc ccg gtc cag atg 960 Val Arg Pro Gln Pro Asn Asp Pro Ser Asn Leu Pro Pro Val Gln Met cta ttc tgc ctc aag cag.
- aag aac agc aag aaa atc aat tcc cac cgg 1008 Leu Phe Cys Leu Lys Gln Lys Asn Ser Lys Lys Ile Asn Ser His Arg tgg ctg ttc aac gcc ttt agt cga atc ctg aat ccg gaa ata tgc atc 1056 Trp Leu Phe Asn Ala Phe Ser Arg Ile Leu Asn Pro Glu Ile Cys Ile ctg ctt gat gct ggc acg aag ccc ggg agc aaa tcc ttg ctt gcc cta 1104 Leu Leu Asp Ala Gly Thr Lys Pro Gly Ser Lys Ser Leu Leu Ala Leu tgg gaa gca ttc tat aat gac aaa aca ctg ggc gga gca tgc ggc gaa 1152 Trp Glu Ala Phe Tyr Asn Asp Lys Thr Leu Gly Gly Ala Cys Gly Glu atc cat gcc atg ctg ggc agg ggg tgg cgc aat gtg ctg aac cct cta 1200 Ile His Ala Met Leu Gly Arg Gly Trp Arg Asn Val Leu Asn Pro Leu gtc gca gcg cag aat ttt gag tac aaa att tcc aat att ctt gat aaa 1248 Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu Asp Lys ccg ctg gaa agc gcc ttt ggc tac gtg agt gtg cta ccg ggt gct ttc 1296 Pro Leu Glu Ser Ala Phe Gly Tyr Val Ser Val Leu Pro Gly Ala Phe tcg gca tat cgc tac cgg gcg atc atg ggc cga ccg ttg gag caa tac 1344 Ser Ala Tyr Arg Tyr Arg Ala Ile Met Gly Arg Pro Leu Glu Gln Tyr ttt cat ggg gat cat act ttg tcc aag cgg ctg gga aag aag ggc att 1392 Phe His Gly Asp His Thr Leu Ser Lys Arg Leu Gly Lys Lys Gly Ile gag ggg atg aat atc ttt aaa aag aac atg ttt ctt gca gag gat cgc 1440 Glu Gly Met Asn Ile Phe Lys Lys Asn Met Phe Leu Ala Glu Asp Arg atc cta tgc ttt gaa ctg gtg gcc aaa gct ggg tat aaa tgg cat ctc 1488 Ile Leu Cys Phe Glu Leu Val Ala Lys Ala Gly Tyr Lys Trp His Leu acg tat gtg aaa gca tcc aag gga gag aca gat gtt ccc gag gcg gcg 1536 Thr Tyr Val Lys Ala Ser Lys Gly Glu Thr Asp Val Pro Glu Ala Ala ccc gaa tac atc agt cag cgg cga cga tgg ctc aat ggc tcc ttt gct 1584 Pro Glu Tyr Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ser Phe Ala gcg agt ttg tat tcc atc atg cac ttc gga cgc atc tat aag agt ggt 1632 Ala Ser Leu Tyr Ser Ile Met His Phe Gly Arg Ile Tyr Lys Ser Gly cat agc ttc gtt cga atg ttc ttc ttg cat att cag atg att tac aac 1680 His Ser Phe Val Arg Met Phe Phe Leu His Ile Gln Met Ile Tyr Asn tgc tgc cag ctc atc atg acc tgg ttc tcg ttg gca tcc tac tgg ctg 1728 Cys Cys Gln Leu Ile Met Thr Trp Phe Ser Leu Ala Ser Tyr Trp Leu acc agc tcg gtc atc atg gac ctc gta ggg acg ccc agc tcg cat aac 1776 Thr Ser Ser Val Ile Met Asp Leu Val Gly Thr Pro Ser Ser His Asn aag tac aag gca tgg cca ttc ggc aac gat gcc tcc ccc att gtc aac 1824 Lys Tyr Lys Ala Trp Pro Phe Gly Asn Asp Ala Ser Pro Ile Val Asn ttt ttt gtc aaa tat ggt tat ctc ttg gtg ctg atg ctc caa ttt gtg 1872 Phe Phe Val Lys Tyr Gly Tyr Leu Leu Val Leu Met Leu Gln Phe Val ctg gct ctg gga aac cgc ccc aaa gcc tac acc atg tcg ttc ctc tgg 1920 Leu Ala Leu Gly Asn Arg Pro Lys Ala Tyr Thr Met Ser Phe Leu Trp ttt tct ctg gtg cag ttc tac gtg ctg atc ctg tcc ttc tac ctg gtc 1968 Phe Ser Leu Val Gln Phe Tyr Val Leu Ile Leu Ser Phe Tyr Leu Val gct aat gcc ttc atg ggt ggc atg atc gac ttt gat ttc gac caa ggc 2016 Ala Asn Ala Phe Met Gly Gly Met Ile Asp Phe Asp Phe Asp Gln Gly gtg ggc aac ttc ctc tcc tcc ttc ttc agc tcg act ggt gga ggg att 2064 Val Gly Asn Phe Leu Ser Ser Phe Phe Ser Ser Thr Gly Gly Gly Ile gtc ctg atc gcc ttg gtg tcc act tac ggc att tac att gtc gcg agc 2112 Val Leu Ile Ala Leu Val Ser Thr Tyr Gly Ile Tyr Ile Val Ala Ser att ctc tac atg gac cct tgg cac atc ctg acc agt tcc tgg gca tac 2160 Ile Leu Tyr Met Asp Pro Trp His Ile Leu Thr Ser Ser Trp Ala Tyr ttc ctg ggc atg acc acg tcg atc aat att ctc atg gtg tat gcg ttc 2208 Phe Leu Gly Met Thr Thr Ser Ile Asn Ile Leu Met Val Tyr Ala Phe tgc aac tgg cac gat gtg tcg tgg ggt acg aag ggg tcc gat aag gcg 2256 Cys Asn Trp His Asp Val Ser Trp Gly Thr Lys Gly Ser Asp Lys Ala gac gct ctg ccc tca gcg cag acg aag aag gcc gac gcg agc aag agc 2304 Asp Ala Leu Pro Ser Ala Gln Thr Lys Lys Ala Asp Ala Ser Lys Ser aac ttc atc gag gag atc gat aaa ccg cag gca gac atc gac agc cag 2352 Asn Phe Ile Glu Glu Ile Asp Lys Pro Gln Ala Asp Ile Asp Ser Gln ttc gag gca acg gtc aaa cgg cgg ctg gcc ccg tat cag gag ccc aaa 2400 Phe Glu Ala Thr Val Lys Arg Arg Leu Ala Pro Tyr Gln Glu Pro Lys gaa gac tcg act atc agc ctg.
- gat gat tcc tac cgc aac ttt cgg acc 2448 Glu Asp Ser Thr Ile Ser Leu Asp Asp Ser Tyr Arg Asn Phe Arg Thr agc ctg gtg ttg ctc tgg atc ttg agt aat ttg ctg gtc tct ctg ctc 2496 Ser Leu Val Leu Leu Trp Ile Leu Ser Asn Leu Leu Val Ser Leu Leu att acg agc gac ggc atc agg aag atg tgt ctg acg aac aca tcc aca 2544 Ile Thr Ser Asp Gly Ile Arg Lys Met Cys Leu Thr Asn Thr Ser Thr acg cgg acg cag tat tat ttc caa gtg att ttg tgg gcg aca gct gga 2592 Thr Arg Thr Gln Tyr Tyr Phe Gln Val Ile Leu Trp Ala Thr Ala Gly ctg tct atc ttc cga ttc att ggg tcg atc tac ttc ctg ggc aag tcg 2640 Leu Ser Ile Phe Arg Phe Ile Gly Ser Ile Tyr Phe Leu Gly Lys Ser gga atc tta tgt tgc gta acg cgt cga tga 2670 Gly Ile Leu Cys Cys Val Thr Arg Arg PRT Aspergillus fumigatus 24 Met Gly Thr Pro Arg Pro Tyr Ser Ala His Ser Pro Gln Glu Ser Arg 1 5 10 15 Ser Ser Phe Tyr Ser Gln Pro Ser Gln Ser Pro Thr Gln Pro Thr Tyr 20 25 30 Gly Arg Asp Asp Ala Glu Asp Gln Gln Gln Ser Leu Leu Arg Arg Ser 35 40 45 Leu Ala Ser Pro Asn Gly Trp Ser Tyr Asp Asp Pro Asn Val Ser Thr 50 55 60 Asp Ser Leu Arg Arg Tyr Thr Leu His Asp Pro Gly Ile Thr Ala Phe Ala Pro Pro Tyr Pro Glu Ser Glu Ala Ala Asp Val Arg Ser Ala Arg 85 90 95 Met Ser Gly Tyr Ser Gly Ile Glu Met Asp Ala Trp Gln Arg Arg Gln Gly Val Lys Pro Ser Ala Leu Arg Arg Tyr Gly Thr Arg Lys Ile Asn Leu Val Gln Gly Ser Val Leu Ser Val Asp Tyr Pro Val Pro Ser Ala Ile Gln Asn Ala Ile Gln Ala Glu Tyr Arg Asp Ala Glu Glu Ala Phe His Glu Glu Phe Thr His Met Arg Tyr Thr Ala Ala Thr Cys Asp Pro Asp Glu Phe Thr Leu Arg Asn Gly Tyr Asn Leu Arg Pro Ala Met Tyr Asn Arg His Thr Glu Arg Leu Ile Ala Ile Thr Tyr Tyr Asn Glu Asp Lys Val Leu Thr Ala Arg Thr Leu His Gly Val Met Gln Asn Val Arg Asp Ile Val Asn Leu Lys Lys Ser Glu Phe Trp Asn Lys Gly Gly Pro Ala Trp Gln Lys Ile Val Val Cys Leu Val Phe Asp Gly Ile Glu Pro Cys Asp Lys Asn Thr Leu Asp Val Leu Ala Thr Ile Gly Val Tyr Gln Asp Gly Val Met Lys Lys Asp Val Asp Gly Arg Glu Thr Val Ala His Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val Thr Pro Thr Gln Gln Leu Val Arg Pro Gln Pro Asn Asp Pro Ser Asn Leu Pro Pro Val Gln Met Leu Phe Cys Leu Lys Gln Lys Asn Ser Lys Lys Ile Asn Ser His Arg Trp Leu Phe Asn Ala Phe Ser Arg Ile Leu Asn Pro Glu Ile Cys Ile Leu Leu Asp Ala Gly Thr Lys Pro Gly Ser Lys Ser Leu Leu Ala Leu Trp Glu Ala Phe Tyr Asn Asp Lys Thr Leu Gly Gly Ala Cys Gly Glu Ile His Ala Met Leu Gly Arg Gly Trp Arg Asn Val Leu Asn Pro Leu Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu Asp Lys Pro Leu Glu Ser Ala Phe Gly Tyr Val Ser Val Leu Pro Gly Ala Phe Ser Ala Tyr Arg Tyr Arg Ala Ile Met Gly Arg Pro Leu Glu Gln Tyr Phe His Gly Asp His Thr Leu Ser Lys Arg Leu Gly Lys Lys Gly Ile Glu Gly Met Asn Ile Phe Lys Lys Asn Met Phe Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Leu Val Ala Lys Ala Gly Tyr Lys Trp His Leu Thr Tyr Val Lys Ala Ser Lys Gly Glu Thr Asp Val Pro Glu Ala Ala Pro Glu Tyr Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ser Phe Ala Ala Ser Leu Tyr Ser Ile Met His Phe Gly Arg Ile Tyr Lys Ser Gly His Ser Phe Val Arg Met Phe Phe Leu His Ile Gln Met Ile Tyr Asn Cys Cys Gln Leu Ile Met Thr Trp Phe Ser Leu Ala Ser Tyr Trp Leu Thr Ser Ser Val Ile Met Asp Leu Val Gly Thr Pro Ser Ser His Asn Lys Tyr Lys Ala Trp Pro Phe Gly Asn Asp Ala Ser Pro Ile Val Asn Phe Phe Val Lys Tyr Gly Tyr Leu Leu Val Leu Met Leu Gln Phe Val Leu Ala Leu Gly Asn Arg Pro Lys Ala Tyr Thr Met Ser Phe Leu Trp Phe Ser Leu Val Gln Phe Tyr Val Leu Ile Leu Ser Phe Tyr Leu Val Ala Asn Ala Phe Met Gly Gly Met Ile Asp Phe Asp Phe Asp Gln Gly Val Gly Asn Phe Leu Ser Ser Phe Phe Ser Ser Thr Gly Gly Gly Ile Val Leu Ile Ala Leu Val Ser Thr Tyr Gly Ile Tyr Ile Val Ala Ser Ile Leu Tyr Met Asp Pro Trp His Ile Leu Thr.
- Ser Ser Trp Ala Tyr Phe Leu Gly Met Thr Thr Ser Ile Asn Ile Leu Met Val Tyr Ala Phe Cys Asn Trp His Asp Val Ser Trp Gly Thr Lys Gly Ser Asp Lys Ala Asp Ala Leu Pro Ser Ala Gln Thr Lys Lys Ala Asp Ala Ser Lys Ser Asn Phe Ile Glu Glu Ile Asp Lys Pro Gln Ala Asp Ile Asp Ser Gln Phe Glu Ala Thr Val Lys Arg Arg Leu Ala Pro Tyr Gln Glu Pro Lys Glu Asp Ser Thr Ile Ser Leu Asp Asp Ser Tyr Arg Asn Phe Arg Thr Ser Leu Val Leu Leu Trp Ile Leu Ser Asn Leu Leu Val Ser Leu Leu Ile Thr Ser Asp Gly Ile Arg Lys Met Cys Leu Thr Asn Thr Ser Thr Thr Arg Thr Gln Tyr Tyr Phe Gln Val Ile Leu Trp Ala Thr Ala Gly Leu Ser Ile Phe Arg Phe Ile Gly Ser Ile Tyr Phe Leu Gly Lys Ser Gly Ile Leu Cys Cys Val Thr Arg Arg DNA Aspergillus fumigatus CDS atg att gtc ctc ttc acg tta ctg agg tgg gct cca ata tcc cca gtc 48 Met Ile Val Leu Phe Thr Leu Leu Arg Trp Ala Pro Ile Ser Pro Val 1 5 10 15 ttc tct atg agg acc atg cat gct aat ttg gca cac agg gga att ttc 96 Phe Ser Met Arg Thr Met His Ala Asn Leu Ala His Arg Gly Ile Phe 20 25 30 ttg cct gtc atg atc gtg acc ctc ccg ctc ccg gtt cac ttg aga cgg 144 Leu Pro Val Met Ile Val Thr Leu Pro Leu Pro Val His Leu Arg Arg 35 40 45 cga ttc ccg gct cag atg gta ctt atg ctt caa tgg ttc gcg ttc ggg 192 Arg Phe Pro Ala Gln Met Val Leu Met Leu Gln Trp Phe Ala Phe Gly 50 55 60 atg ttt tcc gtg ctg ctt ata atc cct tgg ctt ttg tgc gtc tac aga 240 Met Phe Ser Val Leu Leu Ile Ile Pro Trp Leu Leu Cys Val Tyr Arg ctg gtg aca cat tca ccg ggc aga acc aag cgt atc aag caa gtt ttg 288 Leu Val Thr His Ser Pro Gly Arg Thr Lys Arg Ile Lys Gln Val Leu 85 90 95 gat gac cga acc gct ccc aaa aca gtt gtt gtt atg cca gtc tat aag 336 Asp Asp Arg Thr Ala Pro Lys Thr Val Val Val Met Pro Val Tyr Lys gaa gcc ccg gaa aca cta ata agg gca atc gat tct gtc gtt gac tgt 384 Glu Ala Pro Glu Thr Leu Ile Arg Ala Ile Asp Ser Val Val Asp Cys gat tat cca gcc aac tgt atc cat gtg ttc ctc tct tac gat ggc tgc 432 Asp Tyr Pro Ala Asn Cys Ile His Val Phe Leu Ser Tyr Asp Gly Cys ctc att gac gaa tcc tat ctt cgg ctg att gaa cac ctt gga att ccg 480 Leu Ile Asp Glu Ser Tyr Leu Arg Leu Ile Glu His Leu Gly Ile Pro att acg ctg gag agc tat cca cag agc ata gac gtg acg tac aaa gac 528 Ile Thr Leu Glu Ser Tyr Pro Gln Ser Ile Asp Val Thr Tyr Lys Asp gcc aga att acg gtc tct cgt ttc aaa cat gga ggg aaa cga cat tgc 576 Ala Arg Ile Thr Val Ser Arg Phe Lys His Gly Gly Lys Arg His Cys cag aag caa acg ttc aga ctg att gac atg gta tat gcg gat tac ctg 624 Gln Lys Gln Thr Phe Arg Leu Ile Asp Met Val Tyr Ala Asp Tyr Leu gag cgc cac gac aac ctt ttc gtg tta ttc att gac tcc gac tgc atc 672 Glu Arg His Asp Asn Leu Phe Val Leu Phe Ile Asp Ser Asp Cys Ile ctt gac cgt gta tgt ctg caa aac ttc atg tac gat atg gag ttg aag 720 Leu Asp Arg Val Cys Leu Gln Asn Phe Met Tyr Asp Met Glu Leu Lys cca ggg agc aaa cac gac atg ttg gca atg acg ggg gtc att acg tcg 768 Pro Gly Ser Lys His Asp Met Leu Ala Met Thr Gly Val Ile Thr Ser act acg gac cga ggc tcg ctc ctc aca ctt ctg cag gac atg gag tat 816 Thr Thr Asp Arg Gly Ser Leu Leu Thr Leu Leu Gln Asp Met Glu Tyr gtc cat ggg caa ctg ttc gag cgc tct gtg gaa tct agc tgc ggc gct 864 Val His Gly Gln Leu Phe Glu Arg Ser Val Glu Ser Ser Cys Gly Ala gtg act tgc ctc ccc ggg gct ctg acg atg ctc cgg ttc tct gcg ttt 912 Val Thr Cys Leu Pro Gly Ala Leu Thr Met Leu Arg Phe Ser Ala Phe cgt aaa atg gcc aag tac tac ttc gcg gac aaa gcc gag caa tgc gag 960 Arg Lys Met Ala Lys Tyr Tyr Phe Ala Asp Lys Ala Glu Gln Cys Glu gac ttt ttt gac tat ggc aag tgt cat ctt gga gaa gat cgc tgg ctc 1008 Asp Phe Phe Asp Tyr Gly Lys Cys His Leu Gly Glu Asp Arg Trp Leu acg cac ctc ttc atg gta ggc gct cgg aaa cgt tat caa atc cag atg 1056 Thr His Leu Phe Met Val Gly Ala Arg Lys Arg Tyr Gln Ile Gln Met tgc gca ggc gcc ttt tgt aag acc gag gca gtg cag aca ttc agc agt 1104 Cys Ala Gly Ala Phe Cys Lys Thr Glu Ala Val Gln Thr Phe Ser Ser ctt tta aag cag cgt cgg cgc tgg ttt ctg ggt ttc ata acc aac.
- gaa 1152 Leu Leu Lys Gln Arg Arg Arg Trp Phe Leu Gly Phe Ile Thr Asn Glu gtg tgt atg ctg act gat gtg cgg ctt tgg aag cgc tac ccc ttg ctc 1200 Val Cys Met Leu Thr Asp Val Arg Leu Trp Lys Arg Tyr Pro Leu Leu tgc ctg gtc cgt ttt atg cag aac acg atc cga aca act gca ttg ctg 1248 Cys Leu Val Arg Phe Met Gln Asn Thr Ile Arg Thr Thr Ala Leu Leu ttt ttt atc atc gcg ctg tca ctt ata aca acc tcg agc agc atc aat 1296 Phe Phe Ile Ile Ala Leu Ser Leu Ile Thr Thr Ser Ser Ser Ile Asn gac ctg ccc gtg ggt ttt att gcc ata tcg ctg gga ctc aac tac gtc 1344 Asp Leu Pro Val Gly Phe Ile Ala Ile Ser Leu Gly Leu Asn Tyr Val ctc atg ttc tac ttg ggt gcg aag ctc aag cgc tac aaa gct tgg ctt 1392 Leu Met Phe Tyr Leu Gly Ala Lys Leu Lys Arg Tyr Lys Ala Trp Leu ttt ccg ctg atg ttt atc ctg aat ccc ttc ttc aac tgg ctg tat atg 1440 Phe Pro Leu Met Phe Ile Leu Asn Pro Phe Phe Asn Trp Leu Tyr Met gtg tat gga atc ctg act gcg ggc cag cgt aca tgg gga gga ccg aga 1488 Val Tyr Gly Ile Leu Thr Ala Gly Gln Arg Thr Trp Gly Gly Pro Arg gcc gat gcc gcc acc gcc gat gag cac act tcg ccc gag gaa gct gtt 1536 Ala Asp Ala Ala Thr Ala Asp Glu His Thr Ser Pro Glu Glu Ala Val gaa ctg gct aag gct caa ggc gat gag ctc aat gtc gat ctg act act 1584 Glu Leu Ala Lys Ala Gln Gly Asp Glu Leu Asn Val Asp Leu Thr Thr ttc cgt tcc aga ggg gat gag aag agc gtt cca atc cat ccc tcg gag 1632 Phe Arg Ser Arg Gly Asp Glu Lys Ser Val Pro Ile His Pro Ser Glu aag atc gat ggg cgc ttc tct gca cca gag ctc cca gac ggt tat gac 1680 Lys Ile Asp Gly Arg Phe Ser Ala Pro Glu Leu Pro Asp Gly Tyr Asp tcg aac ttg aac gac tcc aac gca gcc ctt acc gag ctg atg acg cct 1728 Ser Asn Leu Asn Asp Ser Asn Ala Ala Leu Thr Glu Leu Met Thr Pro ctt ccg agt gtg cct cgg ata ggt atc cat aca tac ccg tcc tcc gat 1776 Leu Pro Ser Val Pro Arg Ile Gly Ile His Thr Tyr Pro Ser Ser Asp tcg atc cta acc tcg gac tcg ctg agc tcg atc cac ctt ccc ctt aag 1824 Ser Ile Leu Thr Ser Asp Ser Leu Ser Ser Ile His Leu Pro Leu Lys gtt gaa gag ctg act ggt gat aat gac aat atg aag ccc tat ccc gat 1872 Val Glu Glu Leu Thr Gly Asp Asn Asp Asn Met Lys Pro Tyr Pro Asp cgg caa cca agg gac acg tcg agt ttg cac cag atg cag agg act tgt 1920 Arg Gln Pro Arg Asp Thr Ser Ser Leu His Gln Met Gln Arg Thr Cys tct aac gga atc gtg gcc agt gat tca tgc tct tca cag gac gat gct 1968 Ser Asn Gly Ile Val Ala Ser Asp Ser Cys Ser Ser Gln Asp Asp Ala tcg gag atg gta aac aag cct gag ata ctg tca cca tca gct cat ata 2016 Ser Glu Met Val Asn Lys Pro Glu Ile Leu Ser Pro Ser Ala His Ile ctg ccg cat cca tca caa gcc acg gag tcg tca tcc ggg gag gat ata 2064 Leu Pro His Pro Ser Gln Ala Thr Glu Ser Ser Ser Gly Glu Asp Ile tac cca ctt cat ttg cca tcg cca cac caa cac gag gca cat ttt gct 2112 Tyr Pro Leu His Leu Pro Ser Pro His Gln His Glu Ala His Phe Ala cct ctc aat gct tca acc cga ggt tca atg gaa gga aac acc cca gag 2160 Pro Leu Asn Ala Ser Thr Arg Gly Ser Met Glu Gly Asn Thr Pro Glu gta cag cga cca cgc cgt aag ctt cca ggg att cca cgg cct atc aga 2208 Val Gln Arg Pro Arg Arg Lys Leu Pro Gly Ile Pro Arg Pro Ile Arg gcc cag aaa gat cct gaa agt atg gtc tag 2238 Ala Gln Lys Asp Pro Glu Ser Met Val PRT Aspergillus fumigatus 26 Met Ile Val Leu Phe Thr Leu Leu Arg Trp Ala Pro Ile Ser Pro Val 1 5 10 15 Phe Ser Met Arg Thr Met His Ala Asn Leu Ala His Arg Gly Ile Phe 20 25 30 Leu Pro Val Met Ile Val Thr Leu Pro Leu Pro Val His Leu Arg Arg 35 40 45 Arg Phe Pro Ala Gln Met Val Leu Met Leu Gln Trp Phe Ala Phe Gly 50 55 60 Met Phe Ser Val Leu Leu Ile Ile Pro Trp Leu Leu Cys Val Tyr Arg Leu Val Thr His Ser Pro Gly Arg Thr Lys Arg Ile Lys Gln Val Leu 85 90 95 Asp Asp Arg Thr Ala Pro Lys Thr Val Val Val Met Pro Val Tyr Lys Glu Ala Pro Glu Thr Leu Ile Arg Ala Ile Asp Ser Val Val Asp Cys Asp Tyr Pro Ala Asn Cys Ile His Val Phe Leu Ser Tyr Asp Gly Cys Leu Ile Asp Glu Ser Tyr Leu Arg Leu Ile Glu His Leu Gly Ile Pro Ile Thr Leu Glu Ser Tyr Pro Gln Ser Ile Asp Val Thr Tyr Lys Asp Ala Arg Ile Thr Val Ser Arg Phe Lys His Gly Gly Lys Arg His Cys Gln Lys Gln.
- Thr Phe Arg Leu Ile Asp Met Val Tyr Ala Asp Tyr Leu Glu Arg His Asp Asn Leu Phe Val Leu Phe Ile Asp Ser Asp Cys Ile Leu Asp Arg Val Cys Leu Gln Asn Phe Met Tyr Asp Met Glu Leu Lys Pro Gly Ser Lys His Asp Met Leu Ala Met Thr Gly Val Ile Thr Ser Thr Thr Asp Arg Gly Ser Leu Leu Thr Leu Leu Gln Asp Met Glu Tyr Val His Gly Gln Leu Phe Glu Arg Ser Val Glu Ser Ser Cys Gly Ala Val Thr Cys Leu Pro Gly Ala Leu Thr Met Leu Arg Phe Ser Ala Phe Arg Lys Met Ala Lys Tyr Tyr Phe Ala Asp Lys Ala Glu Gln Cys Glu Asp Phe Phe Asp Tyr Gly Lys Cys His Leu Gly Glu Asp Arg Trp Leu Thr His Leu Phe Met Val Gly Ala Arg Lys Arg Tyr Gln Ile Gln Met Cys Ala Gly Ala Phe Cys Lys Thr Glu Ala Val Gln Thr Phe Ser Ser Leu Leu Lys Gln Arg Arg Arg Trp Phe Leu Gly Phe Ile Thr Asn Glu Val Cys Met Leu Thr Asp Val Arg Leu Trp Lys Arg Tyr Pro Leu Leu Cys Leu Val Arg Phe Met Gln Asn Thr Ile Arg Thr Thr Ala Leu Leu Phe Phe Ile Ile Ala Leu Ser Leu Ile Thr Thr Ser Ser Ser Ile Asn Asp Leu Pro Val Gly Phe Ile Ala Ile Ser Leu Gly Leu Asn Tyr Val Leu Met Phe Tyr Leu Gly Ala Lys Leu Lys Arg Tyr Lys Ala Trp Leu Phe Pro Leu Met Phe Ile Leu Asn Pro Phe Phe Asn Trp Leu Tyr Met Val Tyr Gly Ile Leu Thr Ala Gly Gln Arg Thr Trp Gly Gly Pro Arg Ala Asp Ala Ala Thr Ala Asp Glu His Thr Ser Pro Glu Glu Ala Val Glu Leu Ala Lys Ala Gln Gly Asp Glu Leu Asn Val Asp Leu Thr Thr Phe Arg Ser Arg Gly Asp Glu Lys Ser Val Pro Ile His Pro Ser Glu Lys Ile Asp Gly Arg Phe Ser Ala Pro Glu Leu Pro Asp Gly Tyr Asp Ser Asn Leu Asn Asp Ser Asn Ala Ala Leu Thr Glu Leu Met Thr Pro Leu Pro Ser Val Pro Arg Ile Gly Ile His Thr Tyr Pro Ser Ser Asp Ser Ile Leu Thr Ser Asp Ser Leu Ser Ser Ile His Leu Pro Leu Lys Val Glu Glu Leu Thr Gly Asp Asn Asp Asn Met Lys Pro Tyr Pro Asp Arg Gln Pro Arg Asp Thr Ser Ser Leu His Gln Met Gln Arg Thr Cys Ser Asn Gly Ile Val Ala Ser Asp Ser Cys Ser Ser Gln Asp Asp Ala Ser Glu Met Val Asn Lys Pro Glu Ile Leu Ser Pro Ser Ala His Ile Leu Pro His Pro Ser Gln Ala Thr Glu Ser Ser Ser Gly Glu Asp Ile Tyr Pro Leu His Leu Pro Ser Pro His Gln His Glu Ala His Phe Ala Pro Leu Asn Ala Ser Thr Arg Gly Ser Met Glu Gly Asn Thr Pro Glu Val Gln Arg Pro Arg Arg Lys Leu Pro Gly Ile Pro Arg Pro Ile Arg Ala Gln Lys Asp Pro Glu Ser Met Val DNA Aspergillus fumigatus CDS atg gcc tac caa ggc tct ggt tct cat tcg ccg cct cac tac gac gat 48 Met Ala Tyr Gln Gly Ser Gly Ser His Ser Pro Pro His Tyr Asp Asp 1 5 10 15 aac ggt cac cga ctt cag gat ctg cct cat ggt tcg tac gaa gaa gag 96 Asn Gly His Arg Leu Gln Asp Leu Pro His Gly Ser Tyr Glu Glu Glu 20 25 30 gcg tcg aga gga ttg cta tcc cac cag cag ggt ccc ttc aca ggg ccg 144 Ala Ser Arg Gly Leu Leu Ser His Gln Gln Gly Pro Phe Thr Gly Pro 35 40 45 ttt gat gac cct cag cag cat ggt tca tct act acc aga ccc gtt tct 192 Phe Asp Asp Pro Gln Gln His Gly Ser Ser Thr Thr Arg Pro Val Ser 50 55 60 gga tac agt ttg agc gag acc tat gcc ccc gaa gcc gca tat cat gat 240 Gly Tyr Ser Leu Ser Glu Thr Tyr Ala Pro Glu Ala Ala Tyr His Asp ccc tat act caa ccg agc cct ggc tcg gtc tac tcg gct caa tct gcg 288 Pro Tyr Thr Gln Pro Ser Pro Gly Ser Val Tyr Ser Ala Gln Ser Ala 85 90 95 gag aat ccg gcg gcg gct ttt ggt gtc cct gga cgt gtc gcg tcc ccc 336 Glu Asn Pro Ala Ala Ala Phe Gly Val Pro Gly Arg Val Ala Ser Pro tat gct cga agt gac act tca tcc aca gag gca tgg cgc cag aga caa 384 Tyr Ala Arg Ser Asp Thr Ser Ser Thr Glu Ala Trp Arg Gln Arg Gln gct cct gga ggc ggc ccc ggt ggg ttg cgg cgt tat gct aca aga aag 432 Ala Pro Gly Gly Gly Pro Gly Gly Leu Arg Arg Tyr Ala Thr Arg Lys gtc aag ttg gtg cag ggt tcc gtc ctg agt gtc gat tat cct gtt ccg 480 Val Lys Leu Val Gln Gly Ser Val Leu Ser Val Asp Tyr Pro Val Pro agc gcg atc cag aat gct att cag gcc aag tac aga aat gat ctc gag 528 Ser Ala Ile Gln Asn Ala Ile Gln Ala.
- Lys Tyr Arg Asn Asp Leu Glu ggt gga agt gag gaa ttt acg cac atg cga tac acc gcg gcc acc tgt 576 Gly Gly Ser Glu Glu Phe Thr His Met Arg Tyr Thr Ala Ala Thr Cys gat ccc aat gaa ttt aca ctg cac aat ggt tac aat ctt cga cct gcg 624 Asp Pro Asn Glu Phe Thr Leu His Asn Gly Tyr Asn Leu Arg Pro Ala atg tac aac aga cat acc gaa ctt ctc att gcg atc acc tat tat aac 672 Met Tyr Asn Arg His Thr Glu Leu Leu Ile Ala Ile Thr Tyr Tyr Asn gaa gac aag aca ctc act tca cgt aca ctg cac ggt gtc atg cag aac 720 Glu Asp Lys Thr Leu Thr Ser Arg Thr Leu His Gly Val Met Gln Asn att cgc gac att gtg aat ctg aaa aag tcc gag ttc tgg aac aag ggt 768 Ile Arg Asp Ile Val Asn Leu Lys Lys Ser Glu Phe Trp Asn Lys Gly ggt cct gcc tgg cag aag att gtg gtc tgc ctg gtt ttt gat ggt atc 816 Gly Pro Ala Trp Gln Lys Ile Val Val Cys Leu Val Phe Asp Gly Ile gac cct tgc gac aag gac act ttg gat gtt ctt gcg aca atc ggt gtc 864 Asp Pro Cys Asp Lys Asp Thr Leu Asp Val Leu Ala Thr Ile Gly Val tac caa gac ggc gtc atg aag cgt gac gtc gat gga aaa gag act gtg 912 Tyr Gln Asp Gly Val Met Lys Arg Asp Val Asp Gly Lys Glu Thr Val gct cac att ttt gaa tat acg act caa ctg tct gtg act ccg aat cag 960 Ala His Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val Thr Pro Asn Gln cag ctg atc cgc ccc acc gac gac ggt ccc agt aca ctt ctt ccg tcc 1008 Gln Leu Ile Arg Pro Thr Asp Asp Gly Pro Ser Thr Leu Leu Pro Ser aag atg atg ttc tgt ttg aag cag aag aac agc aag aag atc aac tcc 1056 Lys Met Met Phe Cys Leu Lys Gln Lys Asn Ser Lys Lys Ile Asn Ser cac aga tgg ctc ttc aat gca ttt ggt cgc att ctc aac ccc gag gtc 1104 His Arg Trp Leu Phe Asn Ala Phe Gly Arg Ile Leu Asn Pro Glu Val tgt att ctt ctt gac gcc ggt aca aag cct ggc ccg aag tca ctt ctg 1152 Cys Ile Leu Leu Asp Ala Gly Thr Lys Pro Gly Pro Lys Ser Leu Leu tcg ctt tgg gaa gcc ttc tat aac gat aaa gat ttg ggt ggt gct tgc 1200 Ser Leu Trp Glu Ala Phe Tyr Asn Asp Lys Asp Leu Gly Gly Ala Cys ggt gag att cac gcc atg ttg ggt aag ggt tgg aag aac ctg atc aac 1248 Gly Glu Ile His Ala Met Leu Gly Lys Gly Trp Lys Asn Leu Ile Asn ccg ctc gtt gcc gct cag aac ttc gaa tat aag att agt aac atc ctg 1296 Pro Leu Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu gac aag ccc ttg gag agt tca ttt ggt tat gtc agc gtc ttg cct ggt 1344 Asp Lys Pro Leu Glu Ser Ser Phe Gly Tyr Val Ser Val Leu Pro Gly gcc ttc tca gct tac cga ttc cgc gca att atg ggc cgc cct ctc gag 1392 Ala Phe Ser Ala Tyr Arg Phe Arg Ala Ile Met Gly Arg Pro Leu Glu cag tat ttc cat ggt gac cac act ctt tca aag caa ctg ggc aag aag 1440 Gln Tyr Phe His Gly Asp His Thr Leu Ser Lys Gln Leu Gly Lys Lys.
- ggt atc gag gga atg aac att ttc aag aag aac atg ttc ttg gcc gaa 1488 Gly Ile Glu Gly Met Asn Ile Phe Lys Lys Asn Met Phe Leu Ala Glu gat cgt att ctt tgt ttt gag ttg gtt gct aaa gct ggt tca aaa tgg 1536 Asp Arg Ile Leu Cys Phe Glu Leu Val Ala Lys Ala Gly Ser Lys Trp cac ctg acc tat gtc aag gcc tcc aag gct gaa acc gac gtg cca gaa 1584 His Leu Thr Tyr Val Lys Ala Ser Lys Ala Glu Thr Asp Val Pro Glu ggc gct ccc gaa ttc att tcg cag cgt cgt cgt tgg ctg aac ggt tcg 1632 Gly Ala Pro Glu Phe Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ser ttt gct gcc ggt att tat tcg ctc atg cac ttt ggt cgg atg tac aag 1680 Phe Ala Ala Gly Ile Tyr Ser Leu Met His Phe Gly Arg Met Tyr Lys agt gga cac aac att gtc cgc atg ttt ttt ctg cac atc caa atg ttg 1728 Ser Gly His Asn Ile Val Arg Met Phe Phe Leu His Ile Gln Met Leu tac aat atc ttc tct acg gtt ctg aca tgg ttt tcc ttg gct tct tac 1776 Tyr Asn Ile Phe Ser Thr Val Leu Thr Trp Phe Ser Leu Ala Ser Tyr tgg ctt act acc acc gtc atc atg gac ttg gtc ggc acg ccg agc gac 1824 Trp Leu Thr Thr Thr Val Ile Met Asp Leu Val Gly Thr Pro Ser Asp aat aac ggt aac aag gct ttc ccg ttc ggc aag act gca act ccc att 1872 Asn Asn Gly Asn Lys Ala Phe Pro Phe Gly Lys Thr Ala Thr Pro Ile atc aat acc ata gtg aaa tat gtc tac ttg gga ttc cta cta ttg caa 1920 Ile Asn Thr Ile Val Lys Tyr Val Tyr Leu Gly Phe.
- Leu Leu Leu Gln ttc atc ctc gcg ctg ggt aac cgt cca aaa gga tcg aag ttc tca tac 1968 Phe Ile Leu Ala Leu Gly Asn Arg Pro Lys Gly Ser Lys Phe Ser Tyr ctc gcg tct ttc gtg gtt ttc ggc atc att cag gtc tac gtg gtt att 2016 Leu Ala Ser Phe Val Val Phe Gly Ile Ile Gln Val Tyr Val Val Ile gat gca ctg tac ctg gtg gtg cgt gcc ttc agt gga agt gct cct atg 2064 Asp Ala Leu Tyr Leu Val Val Arg Ala Phe Ser Gly Ser Ala Pro Met gat ttc act acg gac caa ggc gtt ggc gag ttt ctg aaa tcg ttc ttt 2112 Asp Phe Thr Thr Asp Gln Gly Val Gly Glu Phe Leu Lys Ser Phe Phe tca tcc agc ggc gcc ggt atc att atc att gct ctc gct gct aca ttc 2160 Ser Ser Ser Gly Ala Gly Ile Ile Ile Ile Ala Leu Ala Ala Thr Phe ggt ctc tac ttt gtg gca tct ttt atg tat ctt gac ccc tgg cac atg 2208 Gly Leu Tyr Phe Val Ala Ser Phe Met Tyr Leu Asp Pro Trp His Met ttc acg tcc ttc cct gcc tac atg tgt gtc caa tcg tca tac atc aat 2256 Phe Thr Ser Phe Pro Ala Tyr Met Cys Val Gln Ser Ser Tyr Ile Asn att ctg aac gtc tac gct ttc agc aat tgg cac gac gtc tcg tgg ggt 2304 Ile Leu Asn Val Tyr Ala Phe Ser Asn Trp His Asp Val Ser Trp Gly acc aag ggt tca gac aag gca gac gct cta cct tcc gcc aag acc acc 2352 Thr Lys Gly Ser Asp Lys Ala Asp Ala Leu Pro Ser Ala Lys Thr Thr aag gat gag ggc aaa gag gtt gtc atc gag gaa atc gac aag cct cag 2400 Lys Asp Glu Gly Lys Glu Val Val Ile Glu Glu Ile Asp Lys Pro Gln gct gat atc gac agt cag ttc gag gca acg gtc aag cgt gcc cta aca 2448 Ala Asp Ile Asp Ser Gln Phe Glu Ala Thr Val Lys Arg Ala Leu Thr ccc tat gtg cca cca gtc gag aag gag gaa aag act ctg gaa gac tcg 2496 Pro Tyr Val Pro Pro Val Glu Lys Glu Glu Lys Thr Leu Glu Asp Ser tac aaa agc ttc cga acg aga ctg gtc acg ttt tgg atc ttt agc aac 2544 Tyr Lys Ser Phe Arg Thr Arg Leu Val Thr Phe Trp Ile Phe Ser Asn gcc ttc ttg gcc gtt tgc atc acc agt gac ggt gtg gat aaa ttc ggc 2592 Ala Phe Leu Ala Val Cys Ile Thr Ser Asp Gly Val Asp Lys Phe Gly ttc acg aat tct gct acc gac cgg acg cag cgt ttc ttc cag gct ttg 2640 Phe Thr Asn Ser Ala Thr Asp Arg Thr Gln Arg Phe Phe Gln Ala Leu ctg tgg tcc aac gct gtc gtt gcc ctg ttc cgt ttc atc gga gcc tgc 2688 Leu Trp Ser Asn Ala Val Val Ala Leu Phe Arg Phe Ile Gly Ala Cys tgg ttc ctg ggc aag aca ggt ttg atg tgc tgc ttt gcc cgg cgt tag 2736 Trp Phe Leu Gly Lys Thr Gly Leu Met Cys Cys Phe Ala Arg Arg.
- PRT Aspergillus fumigatus 28 Met Ala Tyr Gln Gly Ser Gly Ser His Ser Pro Pro His Tyr Asp Asp 1 5 10 15 Asn Gly His Arg Leu Gln Asp Leu Pro His Gly Ser Tyr Glu Glu Glu 20 25 30 Ala Ser Arg Gly Leu Leu Ser His Gln Gln Gly Pro Phe Thr Gly Pro 35 40 45 Phe Asp Asp Pro Gln Gln His Gly Ser Ser Thr Thr Arg Pro Val Ser 50 55 60 Gly Tyr Ser Leu Ser Glu Thr Tyr Ala Pro Glu Ala Ala Tyr His Asp Pro Tyr Thr Gln Pro Ser Pro Gly Ser Val Tyr Ser Ala Gln Ser Ala 85 90 95 Glu Asn Pro Ala Ala Ala Phe Gly Val Pro Gly Arg Val Ala Ser Pro Tyr Ala Arg Ser Asp Thr Ser Ser Thr Glu Ala Trp Arg Gln Arg Gln Ala Pro Gly Gly Gly Pro Gly Gly Leu Arg Arg Tyr Ala Thr Arg Lys Val Lys Leu Val Gln Gly Ser Val Leu Ser Val Asp Tyr Pro Val Pro Ser Ala Ile Gln Asn Ala Ile Gln Ala Lys Tyr Arg Asn Asp Leu Glu Gly Gly Ser Glu Glu Phe Thr His Met Arg Tyr Thr Ala Ala Thr Cys Asp Pro Asn Glu Phe Thr Leu His Asn Gly Tyr Asn Leu Arg Pro Ala Met Tyr Asn Arg His Thr Glu Leu Leu Ile Ala Ile Thr Tyr Tyr Asn Glu Asp Lys Thr Leu Thr Ser Arg Thr Leu His Gly Val Met Gln Asn Ile Arg Asp Ile Val Asn Leu Lys Lys Ser Glu Phe Trp Asn Lys Gly Gly Pro Ala Trp Gln Lys Ile Val Val Cys Leu Val Phe Asp Gly Ile Asp Pro Cys Asp Lys Asp Thr Leu Asp Val Leu Ala Thr Ile Gly Val Tyr Gln Asp Gly Val Met Lys Arg Asp Val Asp Gly Lys Glu Thr Val Ala His Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val Thr Pro Asn Gln Gln Leu Ile Arg Pro Thr Asp Asp Gly Pro Ser Thr Leu Leu Pro Ser Lys Met Met Phe Cys Leu Lys Gln Lys Asn Ser Lys Lys Ile Asn Ser His Arg Trp Leu Phe Asn Ala Phe Gly Arg Ile Leu Asn Pro Glu Val Cys Ile Leu Leu.
- Asp Ala Gly Thr Lys Pro Gly Pro Lys Ser Leu Leu Ser Leu Trp Glu Ala Phe Tyr Asn Asp Lys Asp Leu Gly Gly Ala Cys Gly Glu Ile His Ala Met Leu Gly Lys Gly Trp Lys Asn Leu Ile Asn Pro Leu Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu Asp Lys Pro Leu Glu Ser Ser Phe Gly Tyr Val Ser Val Leu Pro Gly Ala Phe Ser Ala Tyr Arg Phe Arg Ala Ile Met Gly Arg Pro Leu Glu Gln Tyr Phe His Gly Asp His Thr Leu Ser Lys Gln Leu Gly Lys Lys Gly Ile Glu Gly Met Asn Ile Phe Lys Lys Asn Met Phe Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Leu Val Ala Lys Ala Gly Ser Lys Trp His Leu Thr Tyr Val Lys Ala Ser Lys Ala Glu Thr Asp Val Pro Glu Gly Ala Pro Glu Phe Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ser Phe Ala Ala Gly Ile Tyr Ser Leu Met His Phe Gly Arg Met Tyr Lys Ser Gly His Asn Ile Val Arg Met Phe Phe Leu His Ile Gln Met Leu Tyr Asn Ile Phe Ser Thr Val Leu Thr Trp Phe Ser Leu Ala Ser Tyr Trp Leu Thr Thr Thr Val Ile Met Asp Leu Val Gly Thr Pro Ser Asp Asn Asn Gly Asn Lys Ala Phe Pro Phe Gly Lys Thr Ala Thr Pro Ile Ile Asn Thr Ile Val Lys Tyr Val Tyr Leu Gly Phe Leu Leu Leu Gln Phe Ile Leu Ala Leu Gly Asn Arg Pro Lys Gly Ser Lys Phe Ser Tyr Leu Ala Ser Phe Val Val Phe Gly Ile Ile Gln Val Tyr Val Val Ile Asp Ala Leu Tyr Leu Val Val Arg Ala Phe Ser Gly Ser Ala Pro Met Asp Phe Thr Thr Asp Gln Gly Val Gly Glu Phe Leu Lys Ser Phe Phe Ser Ser Ser Gly Ala Gly Ile Ile Ile Ile Ala Leu Ala Ala Thr Phe Gly Leu Tyr Phe Val Ala Ser Phe Met Tyr Leu Asp Pro Trp His Met Phe Thr Ser Phe Pro Ala Tyr Met Cys Val Gln Ser Ser Tyr Ile Asn Ile Leu Asn Val Tyr Ala Phe Ser Asn Trp His Asp Val Ser Trp Gly Thr Lys Gly Ser Asp Lys Ala Asp Ala Leu Pro Ser Ala Lys Thr Thr Lys Asp Glu Gly Lys Glu Val Val Ile Glu Glu Ile Asp Lys Pro Gln Ala Asp Ile Asp Ser Gln Phe Glu Ala Thr Val Lys Arg Ala Leu Thr Pro Tyr Val Pro Pro Val Glu Lys Glu Glu Lys Thr Leu Glu Asp Ser Tyr Lys Ser Phe Arg Thr Arg Leu Val Thr Phe Trp Ile Phe Ser Asn Ala Phe Leu Ala Val Cys Ile Thr Ser Asp Gly Val Asp Lys Phe Gly Phe Thr Asn Ser Ala Thr Asp Arg Thr Gln Arg Phe Phe Gln Ala Leu Leu Trp Ser Asn Ala Val Val Ala Leu Phe Arg Phe Ile Gly Ala Cys Trp Phe Leu Gly Lys Thr Gly Leu Met Cys Cys Phe Ala Arg Arg DNA Aspergillus orzae CDS (1)....
- (2751) 29 atg gcc tac caa ccg cct ggt aaa gat aat ggt gct cag tca cca aac 48 Met Ala Tyr Gln Pro Pro Gly Lys Asp Asn Gly Ala Gln Ser Pro Asn 1 5 10 15 tac aac gat agc ggt cat cga ctg gaa gac ctg ccc cat ggc gcc act 96 Tyr Asn Asp Ser Gly His Arg Leu Glu Asp Leu Pro His Gly Ala Thr 20 25 30 tat gaa gaa gaa gct tca aca gga ctg ctt tcc cac caa cag ggc ggt 144 Tyr Glu Glu Glu Ala Ser Thr Gly Leu Leu Ser His Gln Gln Gly Gly 35 40 45 cct ttc ggt ggt cct ttc gac gac cct cat cag cgt ggc acc tcg cct 192 Pro Phe Gly Gly Pro Phe Asp Asp Pro His Gln Arg Gly Thr Ser Pro 50 55 60 gtt cga cct acg tcg gga tac agc ttg act gaa aca tac gct ccg gac 240 Val Arg Pro Thr Ser Gly Tyr Ser Leu Thr Glu Thr Tyr Ala Pro Asp gcg ggt ttt cat gac cct tac agc acg acg ggc tcg gtt tac tcc ggc 288 Ala Gly Phe His Asp Pro Tyr Ser Thr Thr Gly Ser Val Tyr Ser Gly 85 90 95 aac tcg gca gaa aac ccc gcg gct gcc ttt ggc gtc ccg ggt cgt gta 336 Asn Ser Ala Glu Asn Pro Ala Ala Ala Phe Gly Val Pro Gly Arg Val gct tct ccc tac gct cgc agt gaa aca tcc tcc aca gaa gca tgg cgc 384 Ala Ser Pro Tyr Ala Arg Ser Glu Thr Ser Ser Thr Glu Ala Trp Arg cag cgc cag gct cca gga ggt ggt ggc ggt ggt ggc ctc cgt cgt tac 432 Gln Arg Gln Ala Pro Gly Gly Gly Gly Gly Gly Gly Leu Arg Arg Tyr gct acc aga aag gtc aag cta gtt cag ggt tcc gtc ctc agt gtc gat 480 Ala Thr Arg Lys Val Lys Leu Val Gln Gly Ser Val Leu Ser Val Asp tac ccc gtc ccc agt gct atc cag aat gcc atc caa gcc aag tac cgt 528 Tyr Pro Val Pro Ser Ala Ile Gln Asn Ala Ile Gln Ala Lys Tyr Arg aat gac ctg gag ggt ggc agc gag gag ttt acc.
- cac atg cga tac acc 576 Asn Asp Leu Glu Gly Gly Ser Glu Glu Phe Thr His Met Arg Tyr Thr gct gcg aca tgt gat ccc aat gac ttc acc ctg cac aat ggt tac aat 624 Ala Ala Thr Cys Asp Pro Asn Asp Phe Thr Leu His Asn Gly Tyr Asn ctg cgt ccc gcc atg tat aac aga cac acc gag ttg ttg att gcg att 672 Leu Arg Pro Ala Met Tyr Asn Arg His Thr Glu Leu Leu Ile Ala Ile acc tat tat aac gaa gat aag acc ctt acc gct cgt acc ttg cac ggt 720 Thr Tyr Tyr Asn Glu Asp Lys Thr Leu Thr Ala Arg Thr Leu His Gly gtg atg cag aac att cgc gac att gtg aac ctc aag aag tcc gag ttc 768 Val Met Gln Asn Ile Arg Asp Ile Val Asn Leu Lys Lys Ser Glu Phe tgg aac aaa ggt gga ccc gcc tgg cag aaa att gtt gtc gct ctg gtc 816 Trp Asn Lys Gly Gly Pro Ala Trp Gln Lys Ile Val Val Ala Leu Val ttt gac ggt atc gat cct tgt gat aag gac act ttg gat gtc ctc gcc 864 Phe Asp Gly Ile Asp Pro Cys Asp Lys Asp Thr Leu Asp Val Leu Ala acc atc ggt atc tat cag gac ggt gtc atg aag cgt gac gtc gac ggc 912 Thr Ile Gly Ile Tyr Gln Asp Gly Val Met Lys Arg Asp Val Asp Gly aag gag acc gtg gct cat att ttc gaa tac acg acc caa ttg tcc gtg 960 Lys Glu Thr Val Ala His Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val aca ccg aac cag caa ctc atc cgg ccc act gat gat ggc ccg acc acc 1008 Thr Pro Asn Gln Gln Leu Ile Arg Pro Thr Asp Asp Gly Pro Thr Thr ttg ccc ccg gtg cag atg atg ttc tgc tta aag cag aag aac agc aag 1056 Leu Pro Pro Val Gln Met Met Phe Cys Leu Lys Gln Lys Asn Ser Lys aag atc aac tca cac aga tgg ctg ttc aat gcc ttc ggt cgt att ttg 1104 Lys Ile Asn Ser His Arg Trp Leu Phe Asn Ala Phe Gly Arg Ile Leu aat cca gag gtt tgc atc ctt ctg gat gcc ggt act aag cct ggc cag 1152 Asn Pro Glu Val Cys Ile Leu Leu Asp Ala Gly Thr Lys Pro Gly Gln aag tcc ctc ctg gcg ttg tgg gag ggc ttc tat aac gac aag gat ctg 1200 Lys Ser Leu Leu Ala Leu Trp Glu Gly Phe Tyr Asn Asp Lys Asp Leu gga ggt gct tgt ggt gaa att cac gca atg ttg ggt aaa ggc tgg aag 1248 Gly Gly Ala Cys Gly Glu Ile His Ala Met Leu Gly Lys Gly Trp Lys aat ctg atc aac ccc ctc gtc gcg gcc cag aac ttc gag tac aag atc 1296 Asn Leu Ile Asn Pro Leu Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile agt aat atc ctc gat aaa ccc ttg gag agt tct ttc ggt tat gtc agt 1344 Ser Asn Ile Leu Asp Lys Pro Leu Glu Ser Ser Phe Gly Tyr Val Ser gtg ttg cct ggt gct ttc tcc gcc tat cgt ttc cgt gcc atc atg ggt 1392 Val Leu Pro Gly Ala Phe Ser Ala Tyr Arg Phe Arg Ala Ile Met Gly cga ccc ctc gaa caa tat ttc cat ggt gat cac act ctc tca aaa cag 1440 Arg Pro Leu Glu Gln Tyr Phe His Gly Asp His Thr Leu Ser Lys Gln ctg ggt aag aag ggt att gag ggc atg aac atc ttc aag aag aac atg 1488 Leu Gly Lys Lys Gly Ile Glu Gly Met Asn Ile Phe Lys Lys Asn Met ttc ttg gcc gaa gat cgt atc ctt tgt ttc gaa ctg gtc gcc aag gct 1536 Phe Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Leu Val Ala Lys Ala 500.
- 505 510 ggc tcc aaa tgg cac ttg tcc tac atc aaa gcc tcg aag ggt gaa act 1584 Gly Ser Lys Trp His Leu Ser Tyr Ile Lys Ala Ser Lys Gly Glu Thr gac gtg ccg gaa ggt gtt gct gaa ttc att tcc cag cgt cgt cgt tgg 1632 Asp Val Pro Glu Gly Val Ala Glu Phe Ile Ser Gln Arg Arg Arg Trp ttg aac ggt tct ttt gcg gcc ggt ctc tat tcg ctc atg cat ttc ggt 1680 Leu Asn Gly Ser Phe Ala Ala Gly Leu Tyr Ser Leu Met His Phe Gly cgg atg tac aag agt gga cat aac atc atc cgt atg ttc ttc ttg cac 1728 Arg Met Tyr Lys Ser Gly His Asn Ile Ile Arg Met Phe Phe Leu His att cag atg ttg tac aac gtt ttc aac act atc ctt aca tgg ttc tcc 1776 Ile Gln Met Leu Tyr Asn Val Phe Asn Thr Ile Leu Thr Trp Phe Ser ctg gca tct tac tgg ttg acc acc acc gtc atc atg gac ttg gtc gga 1824 Leu Ala Ser Tyr Trp Leu Thr Thr Thr Val Ile Met Asp Leu Val Gly acg ccc agt gag agc aac ggt aac aaa gga ttc ccc ttc ggt aaa tcg 1872 Thr Pro Ser Glu Ser Asn Gly Asn Lys Gly Phe Pro Phe Gly Lys Ser gcg acc cct att atc aac aca att gtg aag tat gtc tac ctc gga ttg 1920 Ala Thr Pro Ile Ile Asn Thr Ile Val Lys Tyr Val Tyr Leu Gly Leu ttg ctc ttg cag ttc att ctc gct ctc ggt aac cgc ccc aag gga tcc 1968 Leu Leu.
- Leu Gln Phe Ile Leu Ala Leu Gly Asn Arg Pro Lys Gly Ser cgc ttc tcg tac ctg aca tct ttc gtc gta ttc ggt atc att caa atc 2016 Arg Phe Ser Tyr Leu Thr Ser Phe Val Val Phe Gly Ile Ile Gln Ile tac gtt gtc gtc gac gct ctg tac ttg gtg gtt cgt gca ttc aca aac 2064 Tyr Val Val Val Asp Ala Leu Tyr Leu Val Val Arg Ala Phe Thr Asn agt gat gcg ata gat ttc gtc acc gat caa ggt gtt ggc gag ttc ctc 2112 Ser Asp Ala Ile Asp Phe Val Thr Asp Gln Gly Val Gly Glu Phe Leu aag tcg ttc ttc tcg tct tcc ggc gcc ggt atc att atc atc gcc ctg 2160 Lys Ser Phe Phe Ser Ser Ser Gly Ala Gly Ile Ile Ile Ile Ala Leu gct gct act ttc ggt ctc tac ttc gtc gct tcg ttc atg tac ctg gac 2208 Ala Ala Thr Phe Gly Leu Tyr Phe Val Ala Ser Phe Met Tyr Leu Asp cct tgg cat atg ttc acc tcg ttc ccc gcc tac atg ttc gtt cag tca 2256 Pro Trp His Met Phe Thr Ser Phe Pro Ala Tyr Met Phe Val Gln Ser tct tac atc aac gtt ctc aac gtg tac gcg ttc agc aac tgg cac gat 2304 Ser Tyr Ile Asn Val Leu Asn Val Tyr Ala Phe Ser Asn Trp His Asp gtc tcg tgg ggt acc aag ggt tct gat aag gcc gat gcg ctc cct tct 2352 Val Ser Trp Gly Thr Lys Gly Ser Asp Lys Ala Asp Ala Leu Pro Ser gca acg act acg aag gag gat ggc ggc aag gaa gct gtc att gag gaa 2400 Ala Thr Thr Thr Lys Glu Asp Gly Gly Lys Glu Ala Val Ile Glu Glu atc gac aag ccc cag gct gat att gac agc caa ttt gaa gcc act gtc 2448 Ile Asp Lys Pro Gln Ala Asp Ile Asp Ser Gln Phe Glu Ala Thr Val aag cgc gct ctc acc ccc tac gtc ccc cct gtg gag aag gat gag aag 2496 Lys Arg Ala Leu Thr Pro Tyr Val Pro Pro Val Glu Lys Asp Glu Lys tcc ttg gat gat tcc tac aag agt ttc cgt acc cgt ctt gtg acg ttg 2544 Ser Leu Asp Asp Ser Tyr Lys Ser Phe Arg Thr Arg Leu Val Thr Leu tgg atc ttc agt aat gcc ttc ttg gct gta tgc att acc agt gac ggt 2592 Trp Ile Phe Ser Asn Ala Phe Leu Ala Val Cys Ile Thr Ser Asp Gly atg gac aag ttt gga ttc acg aac acc gct acc gac cgt acg tcg cgt 2640 Met Asp Lys Phe Gly Phe Thr Asn Thr Ala Thr Asp Arg Thr Ser Arg ttc ttc cag gct ctc ctg tgg tcc aac gct gct gtc gca ctt gtc cgt 2688 Phe Phe Gln Ala Leu Leu Trp Ser Asn Ala Ala Val Ala Leu Val Arg ttc att ggt gcc tgt tgg ttc ctg ggt aag acg ggt ctc atg tgc tgc 2736 Phe Ile Gly Ala Cys Trp Phe Leu Gly Lys Thr Gly Leu Met Cys Cys ttc gct cgt cgg tag 2751 Phe Ala Arg Arg PRT Aspergillus orzae 30 Met Ala Tyr Gln Pro Pro Gly Lys Asp Asn Gly Ala Gln Ser Pro Asn 1 5 10 15 Tyr Asn Asp Ser Gly His Arg Leu Glu Asp Leu Pro His Gly Ala Thr 20 25 30 Tyr Glu Glu Glu Ala Ser Thr Gly Leu Leu Ser His Gln Gln Gly Gly 35 40 45 Pro Phe Gly Gly Pro Phe Asp Asp Pro His Gln Arg Gly Thr Ser Pro 50 55 60 Val Arg Pro Thr Ser Gly Tyr Ser Leu Thr Glu Thr Tyr Ala Pro Asp Ala Gly Phe His Asp Pro Tyr Ser Thr Thr Gly Ser Val Tyr Ser Gly 85 90 95 Asn Ser Ala Glu Asn Pro Ala Ala Ala Phe Gly Val Pro Gly Arg Val Ala Ser Pro Tyr Ala Arg Ser Glu Thr Ser Ser Thr Glu Ala Trp Arg Gln Arg Gln Ala Pro Gly Gly Gly Gly Gly Gly Gly Leu Arg Arg Tyr Ala Thr Arg Lys Val Lys Leu Val Gln Gly Ser Val Leu Ser Val Asp Tyr Pro Val Pro Ser Ala Ile Gln Asn Ala Ile Gln Ala Lys Tyr Arg Asn Asp Leu Glu Gly Gly Ser Glu Glu Phe Thr His Met Arg Tyr Thr Ala Ala Thr Cys Asp Pro Asn Asp Phe Thr Leu His Asn Gly Tyr Asn Leu Arg Pro Ala Met Tyr Asn Arg His Thr Glu Leu Leu Ile Ala Ile Thr Tyr Tyr Asn Glu Asp Lys Thr Leu Thr Ala Arg Thr Leu His Gly Val Met Gln Asn Ile Arg Asp Ile Val Asn Leu Lys Lys Ser Glu Phe Trp Asn Lys Gly Gly Pro Ala Trp Gln Lys Ile Val Val Ala Leu Val Phe Asp Gly Ile Asp Pro Cys Asp Lys Asp Thr Leu Asp Val Leu Ala Thr Ile Gly Ile Tyr Gln Asp Gly Val Met Lys Arg Asp Val Asp Gly Lys Glu Thr Val Ala His Ile Phe Glu Tyr Thr Thr Gln Leu Ser Val Thr Pro Asn Gln Gln Leu Ile Arg Pro Thr Asp Asp Gly Pro Thr Thr Leu Pro Pro Val Gln Met Met Phe Cys Leu Lys Gln Lys Asn Ser Lys Lys Ile Asn Ser His Arg Trp Leu Phe Asn Ala Phe Gly Arg Ile Leu Asn Pro Glu Val Cys Ile Leu Leu Asp Ala Gly Thr Lys Pro Gly Gln Lys Ser.
- Leu Leu Ala Leu Trp Glu Gly Phe Tyr Asn Asp Lys Asp Leu Gly Gly Ala Cys Gly Glu Ile His Ala Met Leu Gly Lys Gly Trp Lys Asn Leu Ile Asn Pro Leu Val Ala Ala Gln Asn Phe Glu Tyr Lys Ile Ser Asn Ile Leu Asp Lys Pro Leu Glu Ser Ser Phe Gly Tyr Val Ser Val Leu Pro Gly Ala Phe Ser Ala Tyr Arg Phe Arg Ala Ile Met Gly Arg Pro Leu Glu Gln Tyr Phe His Gly Asp His Thr Leu Ser Lys Gln Leu Gly Lys Lys Gly Ile Glu Gly Met Asn Ile Phe Lys Lys Asn Met Phe Leu Ala Glu Asp Arg Ile Leu Cys Phe Glu Leu Val Ala Lys Ala Gly Ser Lys Trp His Leu Ser Tyr Ile Lys Ala Ser Lys Gly Glu Thr Asp Val Pro Glu Gly Val Ala Glu Phe Ile Ser Gln Arg Arg Arg Trp Leu Asn Gly Ser Phe Ala Ala Gly Leu Tyr Ser Leu Met His Phe Gly Arg Met Tyr Lys Ser Gly His Asn Ile Ile Arg Met Phe Phe Leu His Ile Gln Met Leu Tyr Asn Val Phe Asn Thr Ile Leu Thr Trp Phe Ser Leu Ala Ser Tyr Trp Leu Thr Thr Thr Val Ile Met Asp Leu Val Gly Thr Pro Ser Glu Ser Asn Gly Asn Lys Gly Phe Pro Phe Gly Lys Ser Ala Thr Pro Ile Ile Asn Thr Ile Val Lys Tyr Val Tyr Leu Gly Leu Leu Leu Leu Gln Phe Ile Leu Ala Leu Gly Asn Arg Pro Lys Gly Ser Arg Phe Ser Tyr Leu Thr Ser Phe Val Val Phe Gly Ile Ile Gln Ile Tyr Val Val Val Asp Ala Leu Tyr Leu Val Val Arg Ala Phe Thr Asn Ser Asp Ala Ile Asp Phe Val Thr Asp Gln Gly Val Gly Glu Phe Leu Lys Ser Phe Phe Ser Ser Ser Gly Ala Gly Ile Ile Ile Ile Ala Leu Ala Ala Thr Phe Gly Leu Tyr Phe Val Ala Ser Phe Met Tyr Leu Asp Pro Trp His Met Phe Thr Ser Phe Pro Ala Tyr Met Phe Val Gln Ser Ser Tyr Ile Asn Val Leu Asn Val Tyr Ala Phe Ser Asn Trp His Asp Val Ser Trp Gly Thr Lys Gly Ser Asp Lys Ala Asp Ala Leu Pro Ser Ala Thr Thr Thr Lys Glu Asp Gly Gly Lys Glu Ala Val Ile Glu Glu Ile Asp Lys Pro Gln Ala Asp Ile Asp Ser Gln Phe Glu Ala Thr Val Lys Arg Ala Leu Thr Pro Tyr Val Pro Pro Val Glu Lys Asp Glu Lys Ser Leu Asp Asp Ser Tyr Lys Ser Phe Arg Thr Arg Leu Val Thr Leu Trp Ile Phe Ser Asn Ala Phe Leu Ala Val Cys Ile Thr Ser Asp Gly Met Asp Lys Phe Gly Phe Thr Asn Thr Ala Thr Asp Arg Thr Ser Arg Phe Phe Gln Ala Leu Leu Trp Ser Asn Ala Ala Val Ala Leu Val Arg Phe Ile Gly Ala Cys Trp Phe Leu Gly Lys Thr Gly Leu Met Cys Cys Phe Ala Arg Arg DNA Aspergillus orzae 31 ttc cta ttc tat ccg cct tcc aat tgt ccg cct ctg ttg ttg ttg ttg 48 ttt gga tct ctc ttg ctt gct ttt tcc ttc gtt acg gga gta gtg ctt 96 tcg agt gtg ttt gat ctt tct atc tta ttt cat ttc ttt ttc ttt tga 144 taa ccg aat cgt tta gcc ctt cgc ttt cgc ctc atc ggt tgt aat tat 192 aat ctc tgg gct tta caa acg tcg ctt tgc cca aca ctc gct gct cat 240 aca cat tcc cgt cac tcg ctt cat aac ctg aag aat act acg aca tcg 288 ttt cta acg atc aca gct tct gaa ttt act att aat cac ttc ccc gct 336 cag att gta ttc gca tat cct tca ttc ggc tca cga cca aag tga cgc 384 tag gac cgt gtg cgc ata cct gga tcc ttc acc tag ggg tga agt cag 432 aac tga aaa cag gaa ggc tgt agt ttc atg ctc tga acg tat aag att 480 cgg ttc gtc gtt cgt tcc atc gct tgg ttg acg ttc att acg aga gca 528 cat cgg tga tag acc gcc gat taa cct cgg ggt tac aca acc atg tca 576 aat cgt tat tca gtt tac tcc agc cat tcg gct gct ttc tcc acc ggt 624 ggc cga gcg ccc cag gca ggt ggg cag gtg tca acg acg acg ttg ctg 672 aat gcg ttg cat gcg cac tat acg acg gga caa ccc tac caa ctc gac 720 gcg gga acc agt cta gtc gtt aat aca ttg ctt act gca aca caa tcg 768 tcc cca gaa ggc cat acc ggg cct aca ata gat cac gaa cta gca gtc 816 cga gcc tgg gaa cat gca agg aga aga gca gag gac ggt tgt att gtt 864 cta tgg tta gcg ttc atc ccg aga tcc agc agt atc gga aga tca act 912 aac att caa tca taa cca gtt ctg ccc acc act cag cac cat cca ttt 960 tag agc cat tct tag cag cac tac ctt tgt cca ctc cca gta tcg cct 1008 tca cag cgt tgg ccg ctc tcc gtc cct ttt tga cgg ctg taa cca cct 1056 tca acc ctt cat act cgc tct att ccg cgc ttt cag cat gct ata act 1104 tga ccc tca agg gcg aca tca ccg ggc tat ctc tag cac ttt cga cat 1152 cag gga tca atg tcc gca aag gat tcc tcg aca ttc ctt ctg agc cgg 1200 ggt atc gtg cct tcg atg tgt tct att atc tct taa cgt ctg cat cga 1248 cac cag ctg agc ggg agt ttt tgg acc tga gag acg cct ctt cct acg.
- acg ctc gta ttg ccg gta tct gtg gtg 4416 aga caa gtt tgc aaa atg agg aac gaa gct ggt gga cca tga tcc agg 4464 ttt atg agt act aca ttt ctc atc atc tct caa aag cat tcg aat ctc 4512 tct ttg gca gtg taa ctt gtc ttc ctg gat ggt aag tac aac aca aca 4560 ttg caa tat aca tca cgc aga tgc tga ctc gta cag ttt ctg tat gta 4608 tcg ctt gag gac ggc gga caa agg ccg gcc ctt gat cat atc aga caa 4656 ggt tat caa aga ata tgc aga caa cga cgt gga cac gct gca caa gaa 4704 aaa tct gct ttc ttt ggg tga gga tcg tta ctt gac tac att gat gac 4752 gaa gca ctt ccc tac cat gtc cta caa att cat ccc gga tgc cta cgc 4800 tag cac cgc cgc ccc cga gac gtt ctc cgt cct gct gtc tca gcg acg 4848 tcg ctg gat caa ctc cac tgt cca taa ctt ggt gga act cgc tgc tct 4896 gaa aga cct ctg cgg ctt ctg ctg ctt cag tat gcg ctt tgt cgt att 4944 ggt cga tct tct tgg aac tat cat cct ccc agc tac ctg cgt cta ctt 4992 ggg tta cct aat cta cag tgt tgc cag tgg tgg gcc aat tcc aat cat 5040 atc tat cgc cat ctt ggc tgg tgt gta cgg cct cca ggc gat tat ctt 5088 tat tgt gaa gcg gca gtg gca gca tat tgg ttg gat gat cat tta tat 5136 ctg tgc cta tcc gat cta tag ttt cgt tct gcc gat gta ttc ctt ctg 5184 gaa aca gga cga ctt cag ctg ggg taa cac tcg tgt tgt tct tgg aga 5232 gaa ggg aaa taa gcg agt tgt tgc agt aga aga tga acc att cga ccc 5280 tcg cag tat tcc tct cca gcg ctg gga cga tta cgc tct tgc caa taa 5328 tct gcc tgg ccg ccg tgg aga tta taa cat gag cca gga gaa att cta 5376 cgg agg tca ata tgg aga tat ggg cat gga gat gga tga tat gca ttc 5424 cca gta ttc ctc ggt caa gcc tgc atc cac aat ctt aac cgg att tcc 5472 agg agc agg ccg gaa tgg tag tcc tta cat gcc gcc gca gtc gcc cgc 5520 ccc gtt cgg tgg aaa tac ccc agg caa cag gca ttc gca cct gtc cag 5568 ctt tag tcg gta tac cga tat gcc gct tca gcc agg gca cca gtc tcg 5616 aaa cct atc ggt ggg aaa tct cag cca att cca gga tcc gtc gaa ccg 5664 gca tag cgt ggg act cat gca gag cac tga caa tct cct ggg ggt tcc 5712 ccg gcc aaa ctc tcg gag ccc tgt agg cgg tta tac ctc ccg gcc tca 5760 aag tgc gtt tga ctt ccg cgg aag tgg tgg gcc tga tga aat ggc cat 5808 cac gga tgc tat tcg tag ctg cct ggc cga agt gga ctt gga cac tgt 5856 aac gaa gaa gca agg tga gca aat gaa tac agc cgt gtg atg aga tac 5904 atg cta act aaa cta acc aca gtt cgc gca ttg gtc gag cag cgg ctt 5952 caa gcg aca ttg acc gga gac aag cga gca ttc ctc gat cgc cag att 6000 gac cag gaa ttg gca aat atg taa att tgt aga gtg ttt tct ctt cct 6048 tgt ttg ttc ttg cac ccg gta cct taa tac ata tat tcg tgc ggg aaa 6096 atg ttg ata ttt cgg ctt ggc atg cat ggc gtt tgg gat gat ctg aag 6144 cat tca gcg aat ttg aac gag tgt aat agt tgt tta tga gag ttg gaa 6192 tgt act ctg ggt gtt cac ctg tac atg gag cat act aat gtt gtc gga 6240 cag cat cta atg atg aat ttg caa cat gca tgt gtt ata cat ggt aga 6288 tta tag aga tct gga ata ttc aac gcc cat tca PRT Aspergillus orzae 32 Met Ser Asn Arg Tyr Ser Val Tyr Ser Ser His Ser Ala Ala Phe Ser 1 5 10 15 Thr Gly Gly Arg Ala Pro Gln Ala Gly Gly Gln Val Ser Thr Thr Thr 20 25 30 Leu Leu Asn Ala Leu His Ala His Tyr Thr Thr Gly Gln Pro Tyr Gln 35 40 45 Leu Asp Ala Gly Thr Ser Leu Val Val Asn Thr Leu Leu Thr Ala Thr 50 55 60 Gln Ser Ser Pro Glu Gly His Thr Gly Pro Thr Ile Asp His Glu Leu Ala Val Arg Ala Trp Glu His Ala Arg Arg Arg Ala Glu Asp Gly Cys 85 90 95 Ile Val Leu Cys Ser Ala His His Ser Ala Pro Ser Ile Leu Glu Pro Phe Leu Ala Ala Leu Pro Leu Ser Thr Pro Ser Ile Ala Phe Thr Ala Leu Ala Ala Leu Arg Pro Phe Leu Thr Ala Val Thr Thr Phe Asn Pro Ser Tyr Ser Leu Tyr Ser Ala Leu Ser Ala Cys Tyr Asn Leu Thr Leu Lys Gly Asp Ile Thr Gly Leu Ser Leu Ala Leu Ser Thr Ser Gly Ile Asn Val Arg Lys Gly Phe Leu Asp Ile Pro Ser Glu Pro Gly Tyr Arg Ala Phe Asp Val Phe Tyr Tyr Leu Leu Thr Ser Ala Ser Thr Pro Ala Glu Arg Glu Phe Leu Asp Leu Arg Asp Ala Ser Ser Tyr Ala Leu Leu Arg Lys Ser Gly Thr Tyr Thr Pro Pro Ser Tyr Leu Pro Thr Ala Asp Asp Ala Ala Ala Ala Glu Asp Phe Arg Ser Ala Leu Lys Ala Ile Gly Ile Lys Gly Ala Ser Gln Arg Gly Leu Leu Ser Val Leu Ala Gly Leu Leu Lys Leu Gly Asn Ala Ala Gly Phe Leu Val Asp Gln Glu Asp Leu Glu Glu Ala Cys Glu Asp Val Gly Gly Leu Leu Gly Ile Asp Pro Glu Val Leu Leu His Lys Cys Ser Thr Asp Asp Arg Glu Val Leu Ile Ser Gly Ile Tyr Glu Ala Leu Val Asp Trp Val Ile Gly Lys Ala Asn Glu Ala Ile Ala Ser Gln Leu Gln Ala Ser Leu Asp Asp Ser Ser Arg Gly 340 345.
- 350 Ser Gly Gln Ala Ala Gln Trp Thr Asp Asp Asp Thr Val Ser Ile Thr Val Val Asp Leu Pro Arg Pro Ala Leu Gly Lys A.
- a Val Ala Met Arg Gly Ile Phe Asp Asp Thr Leu Gly Ile Asn Ala Glu Met Lys Asp Asp Gly Val Val Val Pro Pro Ala Gly Pro Ala Val Leu Asn Asp Met Thr Ala Ala Ile Ala Gln Val Glu Val Asp Leu Gly Ile Thr Thr Gly Pro Thr Trp Arg Glu Arg Glu Tyr Glu Leu Asp Lys Lys His Glu Val Leu Glu Lys Val Gly Leu Glu Val Glu Met Asp Ser Phe Leu Arg Gln Ile Leu Phe Thr Ala Glu Ser Glu Gly Ile Thr Leu Gly Lys Lys Gly Arg Phe Asp Leu Ala Thr Thr Leu Gly Ser Ser Arg Val Trp His His Ile Ser Ile His Pro Thr Asp Asp Leu Pro Glu Asn Leu Ser Pro Gly Val Pro Thr Ala Ala Trp Ser Ala Gly Ala Val Ser Arg Gln Leu Arg Glu Trp Arg Leu Ala Glu Trp Ala Asn Arg Arg Leu Lys Gln Ile Asp Phe Thr Ala Asp Phe Asp Ile Glu Glu Phe Ile Gly Arg Tyr Phe Arg Leu Gly Cys Gly Glu Gly Lys Asp Gly Val Glu Asn Trp Leu Val Glu Arg Gly Trp Ile Asn Gly Asp Ala Val Val Gly His Gln Arg Ile Trp Val Arg Glu Asn Ala Trp Trp Glu Ala Glu Thr Met Leu Asp Leu Lys Pro Glu Glu Pro Pro Ala Ala Ser Pro Phe Met Tyr Gly Gly Gly Met Leu Asp Pro Gly Val Pro His Tyr Ala Val Pro Pro Ile Ala Glu Ser Thr Ser Leu Leu Gly Ser Arg Asp Asn Leu Leu Asn Arg Gln Ser Thr Leu Val Pro Ser Val Ala Gly Gly Ala Lys Ser Ile Ala Pro Ser Ala Pro His Thr Leu His Thr Gly Gly Asp Tyr Gly Leu Gly Thr Lys Gly Asp Asp Lys Lys Tyr Asp Ser His Pro Tyr Tyr Asp Asp Glu Gly Arg Tyr Leu Gly Glu Leu Asp Pro Glu Tyr Ala Asp Pro Lys His Ile Glu Lys Lys Glu Ile Thr Leu Gly Arg Arg Ile Trp Thr Gly Phe Val Trp Ala Leu Thr Phe Trp Ile Pro Ser Phe Val Leu Arg Phe Val Gly Arg Met Lys Arg Pro Asp Val Arg Met Ala Trp Arg Glu Lys Leu Val Leu Val Phe Leu Ile Leu Leu Phe Asn Ala Ile Val Cys Phe Tyr Ile Ile Ala Phe Gly Asn Leu Leu Cys Pro Asn Lys Asp Lys Val Trp Asn Glu Lys Glu Val Ser Tyr His Gln Gly Asn Asn Asp Phe Tyr Val Ser Ile His Gly Lys Val Tyr Asp Ile Ser Lys Phe Trp Lys Ile Gln His Ser Asp Thr Ser Ile Glu Thr Thr Thr Ser Asn Met Glu Pro Phe Met Gly Glu Asn Leu Asp Ala Tyr Phe Pro Pro Pro Leu Thr Arg Leu Cys Gly Asp Phe Val Thr Asp Glu Ser Ile Thr Leu Arg Asn Asn Asp Thr Asn Ala Val Leu Tyr Ser Asn Ala Lys His Ser Cys Gly Pro Leu Gln Gln Thr Asp Pro Asn Thr Ala Leu His Lys Ile Thr Trp Tyr Glu Asp Val Phe Leu Pro Lys Ile Asp Glu Tyr Tyr Lys Gly Ser Leu Val Trp Lys Arg Ser Glu Val Ser Lys Gln Ala Asp Ser Ser Ser Arg Tyr Trp Val Ile Lys Asp Glu Ser Ile Tyr Asp Leu Thr Asp Tyr Phe Tyr Thr Leu Lys Gln Met Asn Asn Ile Asp Ser Tyr Asn Phe Leu Pro Ser Ser Ile Thr Glu Leu Phe Lys Asn Tyr Pro Gly Thr Asp Val Thr Asp Lys Trp Pro Asn Ser Glu Asn Ala Thr Lys Ala Gln Thr Cys Leu Asp Tyr Val Phe Tyr Lys Gly Lys Val Asp Phe Arg Asp Ser Ala Arg Cys Gln Val Asn Asn Tyr Ile Leu Leu Ala Phe Thr Cys Leu Ile Cys Ala Val Ile Leu Val Lys Phe Leu Ala Ala Leu Gln Leu Gly Ser Lys Arg Arg Pro Ala Pro Gln Asp Lys Phe Val Ile Cys Leu Val Pro Ala Tyr Thr Glu Gly Glu Asp Ser Leu Arg Lys Gly Leu Asp Ser Leu Thr Ala Leu Gln Tyr Asp Asn Lys Arg Lys Leu Ile Tyr Val Ile Cys Asp Gly Met Ile Val Gly Gly Gly Asn Asp Arg Pro Thr Pro Lys Ile Val Leu Asp Ile Leu Gly Val Asp Pro Lys Ile Asp Pro Pro Ala Leu Pro Phe Lys Ser Ile Gly Gln Gly Ser Asp Gln Leu Asn Tyr Gly Lys Val Tyr Ser Gly Leu Tyr Glu Tyr Glu Gly Asn Val Val Pro Tyr Ile Val Val Val Lys Val Gly Lys Glu Ser Glu Gln Ser Lys Ser Lys Pro Gly Asn Arg Gly Lys Arg Asp Ser Gln Ile Gln Ile Met Asn Phe Leu Asn Arg Val His His 1185.
- Arg Ala Pro Met Ser Pro Leu Glu Leu Glu Ile Phe His Gln Ile Asn Asn Val Ile Gly Val Asp Pro Glu Leu Tyr Glu Tyr Cys Leu Met Val Asp Ala Asp Thr Ser Val Arg Glu Asp Ser Leu Asn Arg Leu Val Ala Ala Cys Ala Asn Asp Ala Arg Ile Ala Gly Ile Cys Gly Glu Thr Ser Leu Gln Asn Glu Glu Arg Ser Trp Trp Thr Met Ile Gln Val Tyr Glu Tyr Tyr Ile Ser His His Leu Ser Lys Ala Phe Glu Ser Leu Phe Gly Ser Val Thr Cys Leu Pro Gly Cys Phe Cys Met Tyr Arg Leu Arg Thr Ala Asp Lys Gly Arg Pro Leu Ile Ile Ser Asp Lys Val Ile Lys Glu Tyr Ala Asp Asn Asp Val Asp Thr Leu His Lys Lys Asn Leu Leu Ser Leu Gly Glu Asp Arg Tyr Leu Thr Thr Leu Met Thr Lys His Phe Pro Thr Met Ser Tyr Lys Phe Ile Pro Asp Ala Tyr Ala Ser Thr Ala Ala Pro Glu Thr Phe Ser Val Leu Leu Ser Gln Arg Arg Arg Trp Ile Asn Ser Thr Val His Asn Leu Val Glu Leu Ala Ala Leu Lys Asp Leu Cys Gly Phe Cys Cys Phe Ser Met Arg Phe Val Val Leu Val Asp Leu Leu Gly Thr Ile Ile Leu Pro Ala Thr Cys Val Tyr Leu Gly Tyr Leu Ile Tyr Ser Val Ala Ser Gly Gly Pro Ile Pro Ile Ile Ser Ile Ala Ile Leu Ala Gly Val Tyr Gly Leu Gln Ala Ile Ile Phe Ile Val Lys Arg Gln Trp Gln His Ile Gly Trp Met Ile Ile Tyr Ile Cys Ala Tyr Pro Ile Tyr Ser Phe Val Leu Pro Met Tyr Ser Phe Trp Lys Gln Asp Asp Phe Ser Trp Gly Asn Thr Arg Val Val Leu Gly Glu Lys Gly Asn Lys Arg Val Val Ala Val Glu Asp Glu Pro Phe Asp Pro Arg Ser Ile Pro Leu Gln Arg Trp Asp Asp Tyr Ala Leu Ala Asn Asn Leu Pro Gly Arg Arg Gly Asp Tyr Asn Met Ser Gln Glu Lys Phe Tyr Gly Gly Gln Tyr Gly Asp Met Gly Met Glu Met Asp Asp Met His Ser Gln Tyr Ser Ser Val Lys Pro Ala Ser Thr Ile Leu Thr Gly Phe Pro Gly Ala Gly Arg Asn Gly Ser Pro Tyr Met Pro Pro Gln Ser Pro Ala Pro Phe Gly Gly Asn Thr Pro Gly Asn Arg His Ser His Leu Ser Ser Phe Ser Arg Tyr Thr Asp Met Pro Leu Gln Pro Gly His Gln Ser Arg Asn Leu Ser Val Gly Asn Leu Ser Gln Phe Gln Asp Pro Ser Asn Arg His Ser Val Gly Leu Met Gln Ser Thr Asp Asn Leu Leu Gly Val Pro Arg Pro Asn Ser Arg Ser Pro Val Gly Gly Tyr Thr Ser Arg Pro Gln Ser Ala Phe Asp Phe Arg Gly Ser Gly Gly Pro Asp Glu Met Ala Ile Thr Asp Ala Ile Arg Ser Cys Leu Ala Glu Val Asp Leu Asp Thr Val Thr Lys Lys Gln Val Arg Ala Leu Val Glu Gln Arg Leu Gln Ala Thr Leu Thr Gly Asp Lys Arg Ala Phe Leu Asp Arg Gln Ile Asp Gln Glu Leu Ala Asn Met DNA Aspergillus orzae 33 cct gac tgg att cga ata tcc tcc cag agt aat aat cat aat cac cgc 48 ggt ttc aac cat cgt tcc atc cat ctg caa gtc att ctc cag ata cga 96 tcg cta caa ttc tct tcc acg cca ctc tcg ctt tga aat tcg cca gct 144 gtt cat ctc gca ttc act gca cca aat ttc cgc aga aaa tca acg agc 192 cgc gtg ctg ttt gag gac ccg ttt gcc gtc taa gca atg tgc cta tct 240 ttt ctc tat tga taa gac ata ttc tcc att cca ggg cgt tcc cac att 288 cca agc cat cca gca cct cat cta aat cag cat aac tga acg gcc tta 336 tcc agt tcg aga aca cgc cag gca aga tcg ctt tct ggg ctt ttt ccc 384 tcc gta tat cct tca ccg gca tcc tta ccg atc ttc cgt aag acg ccc 432 ata ccg atc gta act att gtg acc ggt gct taa gaa acc cca gcg tcg 480 agg ttt cga gcc att gat aat atg gtc ggg cct tcg cca gct ggg aca 528 gtt ccg tcc cat gcg cag tcg tcg cta ccc tcg tta cca gcc cat ctg 576 caa tca gac acg cat ttg act gcc cat tta gcc agc cgg tat gat tct 624 gcg atc tac tgt atc tcc att tat aac tga cat cgt ggg tgt tct tct 672 agg ttt cat gtc ggc tta cca aca gct cgc ctg tct tct cag gct ctg 720 atc agt ctc aac aca tac act tca tct tca aag ggc cct gat ggc ggc 768 aag gag ggc agt gct atg ggc gag gcg gaa gac ctc gcc cgt cgt gcc 816 ttc act cgc cta ggt gcc cgt gga gag aat cag gct att gtc ttt ctg 864 taa gcg caa ttt gga cat ata cac acc tac atg ctc tag att cta att 912 gac gat ttg cag cgg gga gag cgg ggc tgg caa aac tac cct tcg agc 960 gca tgt gtt gtc gtc ctt tct ctc gtt ctc gtc gac gcc att gtc atc 1008 caa att gtc gta cgc ctc ctt tat ttt tga cac att gac.
- tga tcg gat gca gat gtg 4176 tct gga taa cct ttt ctt cgt cgg caa ggt tga tac gcg taa ttc agc 4224 tca atg tca att cgc gcg tta ctt cat cct tgc aat ctc aat ctt gat 4272 ttg tgc tgt tgt tat ctt caa att tgc tgc cgc ttt aca att tgg caa 4320 gaa aaa tgt ccc gga gaa tct tga taa att cat cat ttg tca ggt gcc 4368 ggc ata cac tga aga tga gga gtc cct tcg tcg tgc aat gga ttc tat 4416 ggc ccg cat gca gta tga cga caa gcg caa act cct tgt tgt cat ctg 4464 tga tgg tat gat tat tgg aca agg aaa cga tcg acc tac tcc acg gat 4512 tgt att aga tat cct ggg cgt ccc aga atc agt gga tcc cga gcc cct 4560 cag ctt tga gag ttt ggg cga agg cat gaa gca gca caa cat ggg taa 4608 aat tta ttc tgg tct cta tga ggt aca ggg aca tat cgt acc ttt cct 4656 tgt tgt cgt taa agt cgg aaa gcc ttc tga agt ctc ccg acc cgg taa 4704 ccg tgg aaa acg tga ctc cca gat ggt act cat gcg ctt ttt gaa ccg 4752 cgt gca tta caa cct tcc cat gag tcc cat gga act gga aat gta tca 4800 cca aat tcg taa cat cat tgg cgt caa tcc cac gtt cta cga gtt cat 4848 tct gca agt cga cgc tga tac ggt ggt tgc acc cga ttc cgg aac tcg 4896 att cgt cgc ttc ttg cct tgc cga cac acg cat tat cgg tat ctg tgg 4944 cga aac agg ctt gac aaa tgc taa gca ttc agc ggt gac cat gat cca 4992 agt tta cga gta ctt cat ctc cca caa tct gat caa ggc ttt cga aag 5040 tct ttt cgg gtc agt cac atg ttt gcc ggg ctg ctt cac cat gta ccg 5088 tat tcg ttc tgc aga aac cgc aaa acc gct ttt cgt tag caa gga ggt 5136 tgt gga agc cta ctc gga aat tcg cgt cga cac act cca tat gaa gaa 5184 cct gct aca tct ggg tga gga tcg gta ctt aac aac cct tct ctt gaa 5232 gca tca tcc tag ttt caa gac gaa gtt cct att tgc agc taa ggc ctg 5280 gac gat cgc acc tga aag ctt ctc agt ctt ctt atc gca acg tcg tag 5328 atg gat caa ctc aac tgt aca caa tct gat tga act gat ccc tct tca 5376 gca act ttg tgg ttt ctg ttg ctt tag tat gag att cat cgt ctt cgt 5424 tga cct tct cag tac ctg tat cca acc tgt ttc gct cgc cta tat cat 5472 cta ctt gat tgt ctg gct tgc cag aga ttc gtc cac cat tcc atg gac 5520 gtc ttt tgt tct cat cgc agc gat cta tgg tct tca ggc cct cat ctt 5568 cat ctt ccg ccg caa gtg gga aat gat tgg ctg gat gat tgt gta tct 5616 cct ggc cat gcc tat att ctc cgt ggc ctt gcc ctt cta ctc gtt ctg 5664 gca cat gga tga ctt ctc ttg ggg aaa cac ccg cgt cat cac tgg aga 5712 aaa ggg ccg caa agt cgt cat ttc aga tga agg aaa gtt tga ccc cgc 5760 ctc tat ccc gaa aaa gag atg gga gga gta tca agc gga gct ctg gga 5808 ggc cca gac gtc gcg aga cga ccg ctc aga agt ttc agg ctt ctc ata 5856 tgg tac caa gtc gta tca ccc tgc gca gtc cga ata cgg gtt ccc tgg 5904 agc tag acc aat gtc gca gtt cga tct tcc tcg cta tgg gtc cag gat 5952 gtc tct agc tcc ttc cga gat gat gag ccg tca tat gga cat gga aat 6000 gga gga tct ctc aca tct gcc tag cga cga tgc cat tct cgc gga aat 6048 ccg tga aat cct gcg gac agc cga cct gat gac cgt aac gaa gaa gag 6096 tat caa aca aga gct aga gag gcg ttt tgg tgt gaa tct cga tgc caa 6144 gcg tcc ata tat caa ctc agg taa gag atg ttc ccc gtt aag ata tta 6192 cca cag agt ctt act aac tgt ttg tga ccc tta tag cca ccg aag ctg 6240 ttc tat cgg gcg cgc tct aat ctg agg gtc aag ctc gat gtg tat tgc 6288 atg gaa gac cgt tac ctt ttc cac ggt gta taa aaa ttt ctt atc tga 6336 ttt aat att cgc ata tcc att gtc gca act tat aaa ctc gca gct gtg 6384 att gtt tgt PRT Aspergillus orzae 34 Met Val Gly Pro Ser Pro Ala Gly Thr Val Pro Ser His Ala Gln Ser 1 5 10 15 Ser Leu Pro Ser Leu Pro Ala His Leu Gln Ser Asp Thr His Leu Thr 20 25 30 Ala His Leu Ala Ser Arg Phe His Val Gly Leu Pro Thr Ala Arg Leu 35 40 45 Ser Ser Gln Ala Leu Ile Ser Leu Asn Thr Tyr Thr Ser Ser Ser Lys 50 55 60 Gly Pro Asp Gly Gly Lys Glu Gly Ser Ala Met Gly Glu Ala Glu Asp Leu Ala Arg Arg Ala Phe Thr Arg Leu Gly Ala Arg Gly Glu Asn Gln 85 90 95 Ala Ile Val Phe Leu Gly Glu Ser Gly Ala Gly Lys Thr Thr Leu Arg Ala His Val Leu Ser Ser Phe Leu Ser Phe Ser Ser Thr Pro Leu Ser Ser Lys Leu Ser Tyr Ala Ser Phe Ile Phe Asp Thr Leu Thr Thr Thr Lys Ser Leu Thr Thr Leu Thr Ala Ser Lys Ala Gly Leu Phe Leu Glu Leu Gln Tyr Asp Gly Ser Ser Ser Val Asn Pro Thr Leu Ile Gly Gly Lys Ile Ile Asp His Arg Leu Glu Arg Ser Arg Ile Ala Ser Val Pro Thr Gly Glu Arg Ser Phe His Val Leu Tyr Tyr Leu Leu Ala Gly Thr Ser Ala Ala Glu Lys Ser His Leu Gly Phe Asp Asn Ser Ile His Val Ser Thr Asn Ala Gly Lys Leu Ser Ser Ala Ser Ile Gly His Lys Arg Trp Arg Tyr Leu Gly His Pro Thr Gln Leu Lys Val Gly Ile Asn Asp Ala Glu Gly Phe Gln His Phe Lys Thr Ala Leu Arg Lys Leu Glu Phe Pro.
- Arg Ser Glu Ile Ala Glu Ile Cys Gln Ile Leu Ala Val Ile Leu His Ile Gly Gln Leu Asp Phe Ala Ser Gly Gln Ala Thr Leu Thr Ser Ala Glu Glu Ser Gly Gly Tyr Ser His Glu Gly Gly Glu Thr Val Thr Val Val Lys Asn Lys Asp Val Leu Ser Ser Val Ala Ala Phe Leu Gly Leu Gly Val Asp Glu Leu Glu Asn Ser Phe Ser Tyr Arg Thr Lys Thr Ile His Arg Glu Arg Val Thr Val Met Leu Asp Pro Lys Gly Ala Arg Gln Asn Ala Asp Glu Leu Ala Arg Thr Ile Tyr Ser Leu Leu Val Ala Tyr Ile Leu Glu Asn Val Asn Gln Arg Ile Cys Ala Ala Glu Asp Ser Val Ala Asn Thr Val Ser Ile Val Asp Phe Pro Gly Phe Ser Gln Ala Cys Ser Thr Gly Ser Thr Leu Asp Gln Leu Leu Ser Asn Ala Ala Thr Glu Ser Leu Tyr Asn Phe Cys Leu Gln Ser Phe Phe Asp Arg Lys Ala Asp Met Leu Glu Arg Glu Glu Val Val Val Pro Ala Thr Ser Tyr Phe Asp Asn Thr Asp Ala Val Arg Gly Leu Leu Lys His Gly Asn Gly Leu Leu Ser Ile Leu Asp Asp Gln Thr Arg Arg Gly Arg Thr Glu Ala Gln Phe Ala Glu Ser Leu Lys Lys Arg Phe Glu Asn Lys Asn Pro Ala Ile Val Val Gly Ser Ser Gly Ser Thr His Gly Thr Gly Tyr Val Ser Gln Gln Ala Arg Ser Ala Phe Thr Val Lys His Phe Ala Gly Glu Val Asp Tyr Ser Ile Ser Gly Leu Leu Glu Glu Asn Gly Glu Val Ile Ser Gly Asp Leu Met Asn Leu Met Lys Ser Thr Arg Ser Asp Phe Val Arg Glu Leu Phe Gly Gln Glu Ala Leu Gln Thr Val Thr His Pro Lys Glu Lys Thr Ala Ile Met Gln Ala Gln Val Ser Ser Lys Pro Leu Arg Met Pro Ser Met Ala Arg Arg Lys Ala Ser Pro Ala Ser Arg Leu Thr Phe Asp Ala Pro Thr Ala Glu Glu Pro Glu Asp Asn Glu Ser Tyr Gly Gly Ser Thr Ala Lys Ser Ser Gly Arg Arg Lys Ser Ala Met Ser Met Thr Gly Met Gln Gly Ala Ala Gly Gln Phe Leu Ser Ser Leu Glu Ile Val Asn Lys Cys Leu Ser Ser Pro Ser Leu Asn Pro Tyr Phe Ile Phe Cys Leu Lys Pro Asn Asp Arg Arg Ile Ala Asn Gln Phe Asp Ser Lys Cys Val Arg Ala Gln Val Gln Thr Phe Gly Ile Ala Glu Ile Ser Gln Arg Leu Arg Asn Ala Asp Phe Ser Val Phe Leu Pro Phe Glu Glu Phe Leu Gly Leu Ala Glu Val Gly Asn Val Val Val Gly Ser Asp Lys Glu Lys Ser Glu Val Val Leu Asp Glu Lys Arg Trp Pro Gly Asn Glu Ala Arg Val Gly Ser Thr Gly Val Phe Leu Ser Glu Arg Cys Trp Ala Asp Leu Ala Lys Val Gly Glu Arg Val Val Pro Val Tyr His Ala Asp Gly Ser Asp Glu Gly Gly Asp Gly Leu Leu His Pro Arg Thr Ala Gly Tyr Gly Asp Ser Lys Val Arg Leu Leu Asn Pro Ala Asp Gln Ser Leu Gly Asn Phe Ile Tyr Gly Asp Glu Ser Lys Gln Gly Tyr Phe Gly Ser Arg Asp Ile Asp Gly Arg Ser Asp Thr Gly Gly Ser Gly Leu Asn Ser Gly Asp Met Phe His Asn Leu Glu Thr Arg Glu Gln Met Leu Glu Lys Gly Asn Glu Lys Lys Met Glu Glu Val Asp Glu Val Pro Val Ser Gly Ser Arg Lys Arg Trp Met Ala Ile Val Trp Leu Leu Thr Phe Tyr Ile Pro Asp Phe Ala Ile Arg Leu Phe Gly Arg Met Lys Arg Lys Asp Val Arg Thr Ala Trp Arg Glu Lys Phe Ala Ile Asn Leu Ile Ile Trp Phe Ser Cys Ala Val Ala Ile Phe Phe Ile Val Ala Phe Pro Gly Leu Val Cys Pro Thr Gln His Val Tyr Ser Ala Ala Glu Leu Glu Ser His Asn Gly Lys Asn Gly His Asp Ser Tyr Ile Ala Ile Arg Gly Val Val Phe Asp Leu Asp Lys Phe Met Pro Arg His Tyr Pro Asp Ile Val Pro Gln Ser Ser Leu Lys Lys Tyr Ala Gly Met Asp Ala Thr Gly Leu Phe Pro Val Gln Val Ser Ala Leu Cys Gln Gly Lys Asp Gly Ser Ile Asp Pro Thr Val Leu Leu Asp Tyr Thr Pro Thr Asn Ile Ser Gly Ser Ala Thr Thr Ile Ser Thr Gly Asp Leu Asn Ala Lys Tyr His Asp Phe Arg Tyr Tyr Thr Asn Asp Ser Arg Pro Asp Trp Phe Ala Glu Gln Met Lys Glu Leu Arg Ala Thr Tyr Leu Lys Gly Tyr Ile Gly Tyr Thr Pro Gln Tyr Ile Ser Thr Leu Ala Lys Lys Ser Gln Asn Ile Gly Ser Ile Asp Gly Lys Val Tyr Asp Leu Thr Thr Tyr Ile Ser Gly Gly Arg Arg Val Ala Ala Pro Thr 1125.
- 1130 1135 Gly Lys Glu Val Pro Ala Asn Val Asp Arg Glu Phe Met Asp Pro Leu Val Val Ser Leu Phe Gln Asp Leu Pro Gly Gln Asp Leu Ser Lys His Trp Glu Gln Leu Gln Ile Asp Ala Gly Met Arg Asp Arg Met Gln Met Cys Leu Asp Asn Leu Phe Phe Val Gly Lys Val Asp Thr Arg Asn Ser Ala Gln Cys Gln Phe Ala Arg Tyr Phe Ile Leu Ala Ile Ser Ile Leu Ile Cys Ala Val Val Ile Phe Lys Phe Ala Ala Ala Leu Gln Phe Gly Lys Lys Asn Val Pro Glu Asn Leu Asp Lys Phe Ile Ile Cys Gln Val Pro Ala Tyr Thr Glu Asp Glu Glu Ser Leu Arg Arg Ala Met Asp Ser Met Ala Arg Met Gln Tyr Asp Asp Lys Arg Lys Leu Leu Val Val Ile Cys Asp Gly Met Ile Ile Gly Gln Gly Asn Asp Arg Pro Thr Pro Arg Ile Val Leu Asp Ile Leu Gly Val Pro Glu Ser Val Asp Pro Glu Pro Leu Ser Phe Glu Ser Leu Gly Glu Gly Met Lys Gln His Asn Met Gly Lys Ile Tyr Ser Gly Leu Tyr Glu Val Gln Gly His Ile Val Pro Phe Leu Val Val Val Lys Val Gly Lys Pro Ser Glu Val Ser Arg Pro Gly Asn Arg Gly Lys Arg Asp Ser Gln Met Val Leu Met Arg Phe Leu Asn Arg Val His.
- Tyr Asn Leu Pro Met Ser Pro Met Glu Leu Glu Met Tyr His Gln Ile Arg Asn Ile Ile Gly Val Asn Pro Thr Phe Tyr Glu Phe Ile Leu Gln Val Asp Ala Asp Thr Val Val Ala Pro Asp Ser Gly Thr Arg Phe Val Ala Ser Cys Leu Ala Asp Thr Arg Ile Ile Gly Ile Cys Gly Glu Thr Gly Leu Thr Asn Ala Lys His Ser Ala Val Thr Met Ile Gln Val Tyr Glu Tyr Phe Ile Ser His Asn Leu Ile Lys Ala Phe Glu Ser Leu Phe Gly Ser Val Thr Cys Leu Pro Gly Cys Phe Thr Met Tyr Arg Ile Arg Ser Ala Glu Thr Ala Lys Pro Leu Phe Val Ser Lys Glu Val Val Glu Ala Tyr Ser Glu Ile Arg Val Asp Thr Leu His Met Lys Asn Leu Leu His Leu Gly Glu Asp Arg Tyr Leu Thr Thr Leu Leu Leu Lys His His Pro Ser Phe Lys Thr Lys Phe Leu Phe Ala Ala Lys Ala Trp Thr Ile Ala Pro Glu Ser Phe Ser Val Phe Leu Ser Gln Arg Arg Arg Trp Ile Asn Ser Thr Val His Asn Leu Ile Glu Leu Ile Pro Leu Gln Gln Leu Cys Gly Phe Cys Cys Phe Ser Met Arg Phe Ile Val Phe Val Asp Leu Leu Ser Thr Cys Ile Gln Pro Val Ser Leu Ala Tyr Ile Ile Tyr Leu Ile Val Trp Leu Ala Arg Asp Ser Ser Thr Ile Pro Trp Thr Ser Phe Val Leu Ile Ala Ala Ile Tyr Gly Leu Gln Ala Leu Ile Phe Ile Phe Arg Arg Lys Trp Glu Met Ile Gly Trp Met Ile Val Tyr Leu Leu Ala Met Pro Ile Phe Ser Val Ala Leu Pro Phe Tyr Ser Phe Trp His Met Asp Asp Phe Ser Trp Gly Asn Thr Arg Val Ile Thr Gly Glu Lys Gly Arg Lys Val Val Ile Ser Asp Glu Gly Lys Phe Asp Pro Ala Ser Ile Pro Lys Lys Arg Trp Glu Glu Tyr Gln Ala Glu Leu Trp Glu Ala Gln Thr Ser Arg Asp Asp Arg Ser Glu Val Ser Gly Phe Ser Tyr Gly Thr Lys Ser Tyr His Pro Ala Gln Ser Glu Tyr Gly Phe Pro Gly Ala Arg Pro Met Ser Gln Phe Asp Leu Pro Arg Tyr Gly Ser Arg Met Ser Leu Ala Pro Ser Glu Met Met Ser Arg His Met Asp Met Glu Met Glu Asp Leu Ser His Leu Pro Ser Asp Asp Ala Ile Leu Ala Glu Ile Arg Glu Ile Leu Arg Thr Ala Asp Leu Met Thr Val Thr Lys Lys Ser Ile Lys Gln Glu Leu Glu Arg Arg Phe Gly Val Asn Leu Asp Ala Lys Arg Pro Tyr Ile Asn Ser Ala Thr Glu Ala Val Leu Ser Gly Ala Leu 35 906 DNA Saccharomyces cerevisiae CDS atg aaa att ttc aat aca ata caa tct gtg ctg ttc gca gca ttt ttt 48 Met Lys Ile Phe Asn Thr Ile Gln Ser Val Leu Phe Ala Ala Phe Phe 1 5 10 15 cta aaa cag gga aat tgc ctt gcg tca aat ggg agt acc gca ttg atg 96 Leu Lys Gln Gly Asn Cys Leu Ala Ser Asn Gly Ser Thr Ala Leu Met 20 25 30 ggg gaa gta gat atg caa acg ccc ttt cca gag tgg tta aca gaa ttt 144 Gly Glu Val Asp Met Gln Thr.
- Pro Phe Pro Glu Trp Leu Thr Glu Phe 35 40 45 act aat ctt aca caa tgg cct gga att gac cca cct tat att ccg cta 192 Thr Asn Leu Thr Gln Trp Pro Gly Ile Asp Pro Pro Tyr Ile Pro Leu 50 55 60 gat tac ata aat ctt act gaa gtg cca gaa tta gat agg tac tat cct 240 Asp Tyr Ile Asn Leu Thr Glu Val Pro Glu Leu Asp Arg Tyr Tyr Pro ggc cag tgt ccc aaa att tct aga gag cag tgc tca ttt gac tgc tat 288 Gly Gln Cys Pro Lys Ile Ser Arg Glu Gln Cys Ser Phe Asp Cys Tyr 85 90 95 aac tgc atc gat gtt gat gat gta act tcg tgt ttc aaa ctt tcc caa 336 Asn Cys Ile Asp Val Asp Asp Val Thr Ser Cys Phe Lys Leu Ser Gln aca ttt gac gac ggt ccg gcc ccg gcg aca gag gca ttg ctc aag aaa 384 Thr Phe Asp Asp Gly Pro Ala Pro Ala Thr Glu Ala Leu Leu Lys Lys ttg aga caa aga acc act ttt ttt gtt ctg ggg ata aac act gtt aat 432 Leu Arg Gln Arg Thr Thr Phe Phe Val Leu Gly Ile Asn Thr Val Asn tat cct gat ata tat gag cat att tta gag agg ggt cat ttg att ggt 480 Tyr Pro Asp Ile Tyr Glu His Ile Leu Glu Arg Gly His Leu Ile Gly aca cac acg tgg tca cat gaa ttc ttg cca agt tta tca aac gaa gaa 528 Thr His Thr Trp Ser His Glu Phe Leu Pro Ser Leu Ser Asn Glu Glu att gta gcc caa att gaa tgg tca att tgg gct atg aat gcc aca ggc 576 Ile Val Ala Gln Ile Glu Trp Ser Ile Trp Ala Met Asn Ala Thr Gly aaa cat ttc ccc aag tat ttt agg cct cca tac ggt gca att gat aat 624 Lys His Phe Pro Lys Tyr Phe Arg Pro Pro Tyr Gly Ala Ile Asp Asn agg gtt aga gct ata gta aaa cag ttt ggc cta acg gtt gtc ttg tgg 672 Arg Val Arg Ala Ile Val Lys Gln Phe Gly Leu Thr Val Val Leu Trp gat ctc gat act ttt gat tgg aaa tta atc act aat gat gat ttc aga 720 Asp Leu Asp Thr Phe Asp Trp Lys Leu Ile Thr Asn Asp Asp Phe Arg aca gag gaa gaa ata ctt atg gac ata aat act tgg aag gga aaa cgg 768 Thr Glu Glu Glu Ile Leu Met Asp Ile Asn Thr Trp Lys Gly Lys Arg aaa ggt ttg atc tta gag cac gat ggt gca cga aga aca gtt gag gtt 816 Lys Gly Leu Ile Leu Glu His Asp Gly Ala Arg Arg Thr Val Glu Val gct att aaa atc aac gaa ctt att ggt agt gac caa ttg aca att gca 864 Ala Ile Lys Ile Asn Glu Leu Ile Gly Ser Asp Gln Leu Thr Ile Ala gaa tgt att ggt gat aca gac tac atc gaa cgc tac gac tag 906 Glu Cys Ile Gly Asp Thr Asp Tyr Ile Glu Arg Tyr Asp.
- PRT Saccharomyces cerevisiae 36 Met Lys Ile Phe Asn Thr Ile Gln Ser Val Leu Phe Ala Ala Phe Phe 1 5 10 15 Leu Lys Gln Gly Asn Cys Leu Ala Ser Asn Gly Ser Thr Ala Leu Met 20 25 30 Gly Glu Val Asp Met Gln Thr Pro Phe Pro Glu Trp Leu Thr Glu Phe 35 40 45 Thr Asn Leu Thr Gln Trp Pro Gly Ile Asp Pro Pro Tyr Ile Pro Leu 50 55 60 Asp Tyr Ile Asn Leu Thr Glu Val Pro Glu Leu Asp Arg Tyr Tyr Pro Gly Gln Cys Pro Lys Ile Ser Arg Glu Gln Cys Ser Phe Asp Cys Tyr 85 90 95 Asn Cys Ile Asp Val Asp Asp Val Thr Ser Cys Phe Lys Leu Ser Gln Thr Phe Asp Asp Gly Pro Ala Pro Ala Thr Glu Ala Leu Leu Lys Lys Leu Arg Gln Arg Thr Thr Phe Phe Val Leu Gly Ile Asn Thr Val Asn Tyr Pro Asp Ile Tyr Glu His Ile Leu Glu Arg Gly His Leu Ile Gly Thr His Thr Trp Ser His Glu Phe Leu Pro Ser Leu Ser Asn Glu Glu Ile Val Ala Gln Ile Glu Trp Ser Ile Trp Ala Met Asn Ala Thr Gly Lys His Phe Pro Lys Tyr Phe Arg Pro Pro Tyr Gly Ala Ile Asp Asn Arg Val Arg Ala Ile Val Lys Gln Phe Gly Leu Thr Val Val Leu Trp Asp Leu Asp Thr Phe Asp Trp Lys Leu Ile Thr Asn Asp Asp Phe Arg Thr Glu Glu Glu Ile Leu Met Asp Ile Asn Thr Trp Lys Gly Lys Arg Lys Gly Leu Ile Leu Glu His Asp Gly Ala Arg Arg Thr Val Glu Val Ala Ile Lys Ile Asn Glu Leu Ile Gly Ser Asp Gln Leu Thr Ile Ala Glu Cys Ile Gly Asp Thr Asp Tyr Ile Glu Arg Tyr Asp DNA Saccharomyces cerevisiae CDS atg aga ata caa cta aat aca att gat ttg caa tgt att att gca ctt 48 Met Arg Ile Gln Leu Asn Thr Ile Asp Leu Gln Cys Ile Ile Ala Leu 1 5 10 15 tcc tgt ctg ggg caa ttt gtt cac gcg gaa gct aat agg gaa gat tta 96 Ser Cys Leu Gly Gln Phe Val His Ala Glu Ala Asn Arg Glu Asp Leu 20 25 30 aag cag ata gac ttt caa ttt cct gta ttg gaa agg gca gct aca aaa 144 Lys Gln Ile Asp Phe Gln Phe Pro Val Leu Glu Arg Ala Ala Thr Lys 35 40 45 acg cct ttt ccg gat tgg ctt agt gca ttt acc ggg tta aaa gaa tgg 192 Thr Pro.
- Phe Pro Asp Trp Leu Ser Ala Phe Thr Gly Leu Lys Glu Trp 50 55 60 cct ggg tta gat cca cct tat ata cct tta gat ttc att gat ttc agt 240 Pro Gly Leu Asp Pro Pro Tyr Ile Pro Leu Asp Phe Ile Asp Phe Ser caa att cca gat tat aag gaa tat gat caa aac cat tgc gac agt gtt 288 Gln Ile Pro Asp Tyr Lys Glu Tyr Asp Gln Asn His Cys Asp Ser Val 85 90 95 cca agg gac tcg tgc tct ttc gat tgc cat cac tgc acc gaa cac gat 336 Pro Arg Asp Ser Cys Ser Phe Asp Cys His His Cys Thr Glu His Asp gat gtg tac aca tgt tcc aaa ctt tcc cag aca ttt gac gat ggt cct 384 Asp Val Tyr Thr Cys Ser Lys Leu Ser Gln Thr Phe Asp Asp Gly Pro tct gct tcc act act aaa tta ttg gac cgg ttg aag cat aat tcc acc 432 Ser Ala Ser Thr Thr Lys Leu Leu Asp Arg Leu Lys His Asn Ser Thr ttc ttc aat tta ggt gtc aat ata gtt caa cat cca gat atc tat caa 480 Phe Phe Asn Leu Gly Val Asn Ile Val Gln His Pro Asp Ile Tyr Gln aga atg caa aag gag gga cac tta atc ggc tca cat acc tgg tct cac 528 Arg Met Gln Lys Glu Gly His Leu Ile Gly Ser His Thr Trp Ser His gta tat ttg cca aat gta tcg aat gaa aaa att ata gct caa att gaa 576 Val Tyr Leu Pro Asn Val Ser Asn Glu Lys Ile Ile Ala Gln Ile Glu tgg tcc atc tgg gcg atg aat gct act ggc aac cat acc ccc aaa tgg 624 Trp Ser Ile Trp Ala Met Asn Ala Thr Gly Asn His Thr Pro Lys Trp ttc aga cct cca tat ggc gga ata gat aat aga gta aga gca ata aca 672 Phe Arg Pro Pro Tyr Gly Gly Ile Asp Asn Arg Val Arg Ala Ile Thr agg caa ttt ggc tta caa gcc gtc tta tgg gat cac gat act ttt gat 720 Arg Gln Phe Gly Leu Gln Ala Val Leu Trp Asp His Asp Thr Phe Asp tgg agc ctc ctt ctc aat gat tct gtc ata act gaa caa gaa att ctt 768 Trp Ser Leu Leu Leu Asn Asp Ser Val Ile Thr Glu Gln Glu Ile Leu caa aat gta ata aac tgg aac aag tca gga acc gga tta ata tta gaa 816 Gln Asn Val Ile Asn Trp Asn Lys Ser Gly Thr Gly Leu Ile Leu Glu cac gat tca acg gaa aaa act gtc gat ctt gcc att aaa ata aat aag 864 His Asp Ser Thr Glu Lys Thr Val Asp Leu Ala Ile Lys Ile Asn Lys ttg ata ggt gat gat caa tca aca gtt tct cat tgt gtc ggc gga att 912 Leu Ile Gly Asp Asp Gln Ser Thr Val Ser His Cys Val Gly Gly Ile gat tac ata aaa gaa ttc ttg tcc taa 939 Asp Tyr Ile Lys Glu Phe Leu Ser PRT Saccharomyces cerevisiae 38 Met Arg Ile Gln Leu Asn Thr Ile Asp Leu Gln Cys Ile Ile Ala Leu 1 5 10 15 Ser Cys Leu Gly Gln Phe Val His Ala Glu Ala Asn Arg Glu Asp Leu 20 25 30 Lys Gln Ile Asp Phe Gln Phe Pro Val Leu Glu Arg Ala Ala Thr Lys 35 40 45 Thr Pro Phe Pro Asp Trp Leu Ser Ala Phe Thr Gly Leu Lys Glu Trp 50 55 60 Pro Gly Leu Asp Pro Pro Tyr Ile Pro Leu Asp Phe Ile Asp Phe Ser Gln Ile Pro Asp Tyr Lys Glu Tyr Asp Gln Asn His Cys Asp Ser Val 85 90 95 Pro Arg Asp Ser Cys Ser Phe Asp Cys His His Cys Thr Glu His Asp Asp Val Tyr Thr Cys Ser Lys Leu Ser Gln Thr Phe Asp Asp Gly Pro Ser Ala Ser Thr Thr Lys Leu Leu Asp Arg Leu Lys His Asn Ser Thr Phe Phe Asn Leu Gly Val Asn Ile Val Gln His Pro Asp Ile Tyr Gln Arg Met Gln Lys Glu Gly His Leu Ile Gly Ser His Thr Trp Ser His Val Tyr Leu Pro Asn Val Ser Asn Glu Lys Ile Ile Ala Gln Ile Glu Trp Ser Ile Trp Ala Met Asn Ala Thr Gly Asn His Thr Pro Lys Trp Phe Arg Pro Pro Tyr Gly Gly Ile Asp Asn Arg Val Arg Ala Ile Thr Arg Gln Phe Gly Leu Gln Ala Val Leu Trp Asp His Asp Thr Phe Asp Trp Ser Leu Leu Leu Asn Asp Ser Val Ile Thr Glu Gln Glu Ile Leu Gln Asn Val Ile Asn Trp Asn Lys Ser Gly Thr Gly Leu Ile Leu Glu His Asp Ser Thr Glu Lys Thr Val Asp Leu Ala Ile Lys Ile Asn Lys Leu Ile Gly Asp Asp Gln Ser Thr Val Ser His Cys Val Gly Gly Ile Asp Tyr Ile Lys Glu Phe Leu Ser DNA Mucor rouxii 39 tct aca tcc aaa aag aat tga aac gat gca aat caa gac att cgc cct 48 ttc agc tgc aat tgc aca agt tgc tac tct tgc ttt agc cga cac ctc 96 cgc aaa tta ctg gca atc att tac ttc tca aat taa tcc caa gaa cat 144 ctc cat tcc ctc tat tga gca aac ttc atc cat tga ccc cac tca aga 192 atg tgc tta cta cac tcc tga tgc ttc att gtt cac att caa cgc ttc 240 cga atg gcc ctc tat ctg gga agt cgc tac tac caa tgg tat gaa tga 288 gtc tgc cga gtt cct cag tgt cta caa ttc tat tga ctg gac caa ggc 336 acc caa tat ttc tgt gcg tac cct tga cgc taa cgg caa ctt gga tac 384.
- 45 Ser Ser Ile Asp Pro Thr Gln Glu Cys Ala Tyr Tyr Thr Pro Asp Ala 50 55 60 Ser Leu Phe Thr Phe Asn Ala Ser Glu Trp Pro Ser Ile Trp Glu Val Ala Thr Thr Asn Gly Met Asn Glu Ser Ala Glu Phe Leu Ser Val Tyr 85 90 95 Asn Ser Ile Asp Trp Thr Lys Ala Pro Asn Ile Ser Val Arg Thr Leu Asp Ala Asn Gly Asn Leu Asp Thr Thr Gly Tyr Asn Thr Ala Thr Asp Pro Asp Cys Trp Trp Thr Ala Thr Thr Cys Thr Ser Pro Lys Ile Ser Asp Ile Asn Asp Asp Ile Ser Lys Cys Pro Glu Pro Glu Thr Trp Gly Leu Thr Tyr Asp Asp Gly Pro Asn Cys Ser His Asn Ala Phe Tyr Asp Tyr Leu Gln Glu Gln Lys Leu Lys Ala Ser Met Phe Tyr Ile Gly Ser Asn Val Val Asp Trp Pro Tyr Gly Ala Met Arg Gly Val Val Asp Gly His His Ile Ala Ser His Thr Trp Ser His Pro Gln Met Thr Thr Lys Thr Asn Gln Glu Val Leu Ala Glu Phe Tyr Tyr Thr Gln Lys Ala Ile Lys Leu Ala Thr Gly Leu Thr Pro Arg Tyr Trp Arg Pro Pro Tyr Gly Asp Ile Asp Asp Arg Val Arg Trp Ile Ala Ser Gln Leu Gly Leu Thr Ala Val Ile Trp Asn Leu Asp Thr Asp Asp Trp Ser Ala Gly Val Thr Thr Thr Val Glu Ala Val Glu Gln Ser Tyr Ser Asp Tyr Ile Ala Met Gly Thr Asn Gly Thr Phe Ala Asn Ser Gly Asn Ile Val Leu Thr His Glu Ile Asn Thr Thr Met Ser Leu Ala Val Glu Asn Leu Pro Lys Ile Ile Ser Ala Tyr Lys Gln Val Ile Asp Val Ala Thr Cys Tyr Asn Ile Ser His Pro Tyr Phe Glu Asp Tyr Glu Trp Thr Asn Val Leu Asn Gly Thr Lys Ser Ser Ala Thr Ala Ser Gly Ser Ala Thr Ser Ala Ser Ala Ser Gly Gly Ala Thr Thr Ala Ala Ala His Ile Gln Ala Ser Thr Ser Gly Ala Met Ser Val Leu Pro Asn Leu Ala Leu Ile Ser Ala Phe Ile Ala Thr Leu Leu Phe DNA Gongronella butleri CDS atg cgc cgc ctt gga cag ggc atc caa ttg gtg tcc gct gac tat tgg 48 Met Arg Arg Leu Gly Gln Gly Ile Gln Leu Val Ser Ala Asp Tyr Trp 1 5 10 15 tcc aac ttc aat tcg agc gtg aac cct ctc aac gtg aag gtg ccc caa 96 Ser Asn Phe Asn Ser Ser Val Asn Pro Leu Asn Val Lys Val Pro Gln 20 25 30 atc aca cag cca cgt cga ttg atc acg tca cgg agt gca cgg tac tac 144 Ile Thr Gln Pro Arg Arg Leu Ile Thr Ser Arg Ser Ala Arg Tyr Tyr 35 40 45 acg cct gat cca ctc gtt gat cac cat cac gcc cac cgg ctg ggg ccc 192 Thr Pro Asp Pro Leu Val Asp His His His Ala His Arg Leu Gly Pro 50 55 60 aat tgg ctc acg gcc acc acc aat ggc atg aac acc agt gct gaa ttc 240 Asn Trp Leu Thr Ala Thr Thr Asn Gly Met Asn Thr Ser Ala Glu Phe acc gcc ctt tac aac tcg atc aat tgg gac aac ggc ggc cca aac.
- atc 288 Thr Ala Leu Tyr Asn Ser Ile Asn Trp Asp Asn Gly Gly Pro Asn Ile 85 90 95 tcg gtg cgc acc ttc aac acc gat ggc tcc atg aac act aat ggc tac 336 Ser Val Arg Thr Phe Asn Thr Asp Gly Ser Met Asn Thr Asn Gly Tyr gac gtg gcc aat gat ccc gat tgt tgg tgg act gtc tct ggc tgc acg 384 Asp Val Ala Asn Asp Pro Asp Cys Trp Trp Thr Val Ser Gly Cys Thr gtg ccc aag ctc cag gat gtc aat gct gac att tac aag tgt cct gag 432 Val Pro Lys Leu Gln Asp Val Asn Ala Asp Ile Tyr Lys Cys Pro Glu ccc gat acg tgg ggc ttg ttc tat gac gac ggc ccc aat tgc tcg cac 480 Pro Asp Thr Trp Gly Leu Phe Tyr Asp Asp Gly Pro Asn Cys Ser His aat gcc ttt tac aac ttt ttg cag gag caa aat ctg cgc gct tcc atg 528 Asn Ala Phe Tyr Asn Phe Leu Gln Glu Gln Asn Leu Arg Ala Ser Met ttt tac att ggt tcc aac gtc atg aac tgg ccc tat ggt gcc atg cgt 576 Phe Tyr Ile Gly Ser Asn Val Met Asn Trp Pro Tyr Gly Ala Met Arg ggt gtc caa gat ggc cat cac att gct ttc cac acc tgg tcc cat cag 624 Gly Val Gln Asp Gly His His Ile Ala Phe His Thr Trp Ser His Gln tca ttg acg acc ctg acg aac caa gaa ggg ctc gcc gag ttc tac tac 672 Ser Leu Thr Thr Leu Thr Asn Gln Glu Gly Leu Ala Glu Phe Tyr Tyr acg caa aag atg att cac ttg gcc act ggt gtg aca cct cgc tac tgg 720 Thr Gln Lys Met Ile His Leu Ala Thr Gly Val Thr Pro Arg Tyr Trp cgc gct ccc tat ggt gat gtc gat gat cgt gtg cgc tgg att gcc acg 768 Arg Ala Pro Tyr Gly Asp Val Asp Asp Arg Val Arg Trp Ile Ala Thr caa ttg aac ctg acg acg atc ctc tgg gac tat gat acc aac gac tgg 816 Gln Leu Asn Leu Thr Thr Ile Leu Trp Asp Tyr Asp Thr Asn Asp Trp cag gca ggc gac ggt gtg ccc gag tcc acg gtg caa aac acg tac aat 864 Gln Ala Gly Asp Gly Val Pro Glu Ser Thr Val Gln Asn Thr Tyr Asn gaa ttc atc cag atg ggc aac aat ggt tcg atg gcc agc ggt ggc aac 912 Glu Phe Ile Gln Met Gly Asn Asn Gly Ser Met Ala Ser Gly Gly Asn att gta ctg acg cac gag atc aac aac acg acg atg caa ttg gcc gtc 960 Ile Val Leu Thr His Glu Ile Asn Asn Thr Thr Met Gln Leu Ala Val gag aac atc ccc aac atg ctc aag tct tac aag cac gtc gtc aac gtt 1008 Glu Asn Ile Pro Asn Met Leu Lys Ser Tyr Lys His Val Val Asn Val gcc acc tgc atg aac atc acg ttc ccg aca tgg agc aga ccg gtg cct 1056 Ala Thr Cys Met Asn Ile Thr Phe Pro Thr Trp Ser Arg Pro Val Pro ttc cct tct tta gcc aat tta ttg cgc aaa aca gct tgg gtg cgg gcg 1104 Phe Pro Ser Leu Ala Asn Leu Leu Arg Lys Thr Ala Trp Val Arg Ala gtg ccg cct tca aca tta cca cgg gcg ccg ggg ctc aga gct caa gct 1152 Val Pro Pro Ser Thr Leu Pro Arg Ala Pro Gly Leu Arg Ala Gln Ala cgt ttt ctc gtt ggg cgc cat gtc ttt gcc caa gcc aat gcc ggt gtc 1200 Arg Phe Leu Val Gly Arg His Val Phe Ala Gln Ala Asn Ala Gly Val gcc gtt ttg atg aca gtc cgt ggg ccg ttg cgc ttt gca ttt aaa tct 1248 Ala Val Leu Met Thr Val Arg Gly Pro Leu Arg Phe Ala Phe Lys Ser ttt cca atg gga ccc cct ttt ctc cct tcc tta tat gtt ttt tga 1293 Phe Pro Met Gly Pro Pro Phe Leu Pro Ser Leu Tyr Val Phe PRT Gongronella butleri 42 Met Arg Arg Leu Gly Gln Gly Ile Gln Leu Val Ser Ala Asp Tyr Trp 1 5 10 15 Ser Asn Phe Asn Ser Ser Val Asn Pro Leu Asn Val Lys Val Pro Gln 20 25 30 Ile Thr Gln Pro Arg Arg Leu Ile Thr Ser Arg Ser Ala Arg Tyr Tyr 35 40 45 Thr Pro Asp Pro Leu Val Asp His His His Ala His Arg Leu Gly Pro 50 55 60 Asn Trp Leu Thr Ala Thr Thr Asn Gly Met Asn Thr Ser Ala Glu Phe Thr Ala Leu Tyr Asn Ser Ile Asn Trp Asp Asn Gly Gly Pro Asn Ile 85 90 95 Ser Val Arg Thr Phe Asn Thr Asp Gly Ser Met Asn Thr Asn Gly Tyr Asp Val Ala Asn Asp Pro Asp Cys Trp Trp Thr Val Ser Gly Cys Thr Val Pro Lys Leu Gln Asp Val Asn Ala Asp Ile Tyr Lys Cys Pro Glu Pro Asp Thr Trp Gly Leu Phe Tyr Asp Asp Gly Pro Asn Cys Ser His Asn Ala Phe Tyr Asn Phe Leu Gln Glu Gln Asn Leu Arg Ala Ser Met Phe Tyr Ile Gly Ser Asn Val Met Asn Trp Pro Tyr Gly Ala Met Arg Gly Val Gln Asp Gly His His Ile Ala Phe His Thr Trp Ser His Gln Ser Leu Thr Thr Leu Thr Asn Gln Glu Gly Leu Ala Glu Phe Tyr Tyr Thr Gln Lys Met Ile His Leu Ala Thr Gly Val Thr Pro Arg Tyr Trp Arg Ala Pro Tyr Gly Asp.
- Val Asp Asp Arg Val Arg Trp Ile Ala Thr Gln Leu Asn Leu Thr Thr Ile Leu Trp Asp Tyr Asp Thr Asn Asp Trp Gln Ala Gly Asp Gly Val Pro Glu Ser Thr Val Gln Asn Thr Tyr Asn Glu Phe Ile Gln Met Gly Asn Asn Gly Ser Met Ala Ser Gly Gly Asn Ile Val Leu Thr His Glu Ile Asn Asn Thr Thr Met Gln Leu Ala Val Glu Asn Ile Pro Asn Met Leu Lys Ser Tyr Lys His Val Val Asn Val Ala Thr Cys Met Asn Ile Thr Phe Pro Thr Trp Ser Arg Pro Val Pro Phe Pro Ser Leu Ala Asn Leu Leu Arg Lys Thr Ala Trp Val Arg Ala Val Pro Pro Ser Thr Leu Pro Arg Ala Pro Gly Leu Arg Ala Gln Ala Arg Phe Leu Val Gly Arg His Val Phe Ala Gln Ala Asn Ala Gly Val Ala Val Leu Met Thr Val Arg Gly Pro Leu Arg Phe Ala Phe Lys Ser Phe Pro Met Gly Pro Pro Phe Leu Pro Ser Leu Tyr Val Phe DNA.
- Paramecium bursaria Chlorella virus CVK2 CDS atg ctg cct ctc aaa atc aag agt agc ata ctg tat gcg gtt atc ttg 48 Met Leu Pro Leu Lys Ile Lys Ser Ser Ile Leu Tyr Ala Val Ile Leu 1 5 10 15 gcc att aac ttt gga ttg ctt tcg ctc gtc att ata ttt cat gag tat 96 Ala Ile Asn Phe Gly Leu Leu Ser Leu Val Ile Ile Phe His Glu Tyr 20 25 30 tgg tat gca ttt gcg cct att ctt gta ctc ggc gct gcg tct tct ctg 144 Trp Tyr Ala Phe Ala Pro Ile Leu Val Leu Gly Ala Ala Ser Ser Leu 35 40 45 tgg tat att gcg tgg gtg ctt atg cat cgt gta tac tta ggt ttc aaa 192 Trp Tyr Ile Ala Trp Val Leu Met His Arg Val Tyr Leu Gly Phe Lys 50 55 60 gga aaa ccc gtg ctg acc gcc ccc aaa gaa cct atg atg ttc ctc gtc 240 Gly Lys Pro Val Leu Thr Ala Pro Lys Glu Pro Met Met Phe Leu Val aca gcg tat cgc gag acg aag gaa gaa ctt gat aga acc gtg gag tcc 288 Thr Ala Tyr Arg Glu Thr Lys Glu Glu Leu Asp Arg Thr Val Glu Ser 85 90 95 gtt acg atg caa aaa ata gac ccc gag gtt agc aag act gtt gtt gtt 336 Val Thr Met Gln Lys Ile Asp Pro Glu Val Ser Lys Thr Val Val Val att gtt gat ggt gag aag gaa act gca cac gaa cta cga aag tat aac 384 Ile Val Asp Gly Glu Lys Glu Thr Ala His Glu Leu Arg Lys Tyr Asn cag tat gat gaa act ttc gtc atc aaa gat gca tat gag gat tgg cat 432 Gln Tyr Asp Glu Thr Phe Val Ile Lys Asp Ala Tyr Glu Asp Trp His aat aag cca aag gat gtt aca att ttc aag aaa ata cat aat ggt att 480 Asn Lys Pro Lys Asp Val Thr Ile Phe Lys Lys Ile His Asn Gly Ile gac gtc gta tat ctc ata aaa agt gag aac gcg gga aaa cgt gat agc 528 Asp Val Val Tyr Leu Ile Lys Ser Glu Asn Ala Gly Lys Arg Asp Ser gtt gtg ctt gca cga act ctt gca tac gga aat ctg ttc gaa cat agt 576 Val Val Leu Ala Arg Thr Leu Ala Tyr Gly Asn Leu Phe Glu His Ser gaa aac aga cat gct atg aaa att tca ggc gaa tta gac ctc ata tgg 624 Glu Asn Arg His Ala Met Lys Ile Ser Gly Glu Leu Asp Leu Ile Trp tct cgt t 631 Ser Arg PRT Paramecium bursaria Chlorella virus CVK2 44 Met Leu Pro Leu Lys Ile Lys Ser Ser Ile Leu Tyr Ala Val Ile Leu 1 5 10 15 Ala Ile Asn Phe Gly Leu Leu Ser Leu Val Ile Ile Phe His Glu Tyr 20 25 30 Trp Tyr Ala Phe Ala Pro Ile Leu Val Leu Gly Ala Ala Ser Ser Leu 35 40 45 Trp Tyr Ile Ala Trp Val Leu Met His Arg Val Tyr Leu Gly Phe Lys 50 55 60 Gly Lys Pro Val Leu Thr Ala Pro Lys Glu Pro Met Met Phe Leu Val Thr Ala Tyr Arg Glu Thr Lys Glu Glu Leu Asp Arg Thr Val Glu Ser 85 90 95 Val Thr Met Gln Lys Ile Asp Pro Glu Val Ser Lys Thr Val Val Val Ile Val Asp Gly Glu Lys Glu Thr Ala His Glu Leu Arg Lys Tyr Asn Gln Tyr Asp Glu Thr Phe Val Ile Lys Asp Ala Tyr Glu Asp Trp His Asn Lys Pro Lys Asp Val Thr Ile Phe Lys Lys Ile His Asn Gly Ile Asp Val Val Tyr Leu Ile Lys Ser Glu Asn Ala Gly Lys Arg Asp Ser Val Val Leu Ala Arg Thr Leu Ala Tyr Gly Asn Leu Phe Glu His Ser Glu Asn Arg His Ala

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