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Homologous recombination

Tìm thấy 15+ kết quả cho từ khóa "Homologous recombination"

Impact of homologous recombination on core genome phylogenies

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Impact of homologous recombination on core genome phylogenies. Background: Core genome phylogenies are widely used to build the evolutionary history of individual prokaryote species. Few attempts have been made to evaluate the robustness of core genome phylogenies to recombination, but some analyses suggest that reconstructed trees are not always accurate.. Results: In this study, we tested the robustness of core genome phylogenies to various levels of recombination rates.

Comparative genomics reveals broad genetic diversity, extensive recombination and nascent ecological adaptation in Micrococcus luteus

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Recombination, especially homologous recombination, is one of the main forces shaping bacterial evolution [37, 38]. Here, a series of analyses were used to evaluate the extent of homologous recombination in the core genome of M. the recombin- ation coverage (c), which indicates the proportion of sites in the genome whose diversity has come from re- combination events since its last common ancestor, was 0.46. A summary of the parame- ters of M.

Sự chuyển vị Sự chuyển vị (Transposition

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Tái tổ hợp (Recombination) trong chuyển vị (transposition) là sự kết nối (junction) giữa các đầu mút của phần tử chuyển vị với các đầu mút bị cắt hở của ADN mục tiêu (target site). Khác với tái tổ hợp tương đồng (homologous recombination), transposition không đòi hỏi sự tương đồng (homologous sequence) giữa các phần tử di động và ADN mục tiêu, và nó độc lập với chức năng được mã hóa bởi recA, vì nó sử dụng enzym đặc hiệu là Transposaz.

Structural variation of the malariaassociated human glycophorin A-B-E region

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These losses and gains are consistent with an origin by non-allelic homologous recombination (NAHR) between glyco- phorin repeats, with particular involvement of the PRDM9 C allele, which is at appreciable frequencies in African populations and directs high recombination rates at its cognate recombination hotspots.

Genetic and metabolic signatures of Salmonella enterica subsp. enterica associated with animal sources at the pangenomic scale

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In comparison to human GWAS, the confounding factors of the microbial GWAS include genome selection, homologous recombination events, population structure, as well as genome wide sig- nificance [43]. genome selection, homologous recombination events, population structure and genome wide significance), the present study proposes to decipher Salmonella adaptation to animal sources (i.e. Distributions of serovars from potential mono-and multi- animal sources. 1) had single animal sources (p <.

Impacts of local population history and ecology on the evolution of a globally dispersed pathogen

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Previous studies have suggested that homologous recombination leads to homogenization of the core genome in bacteria [71]. The direction of donor/recipient strains within Costa Rica changed with evolutionary time. The low genetic di- versity of the subsp. At a whole genome level, the nucleo- tide diversity in Costa Rica was the highest of all evalu- ated populations (Costa Rica, 14,573 SNPs and π = 3.18x10e − 03 .

Circular DNA intermediates in the generation of large human segmental duplications

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(ii) how could the circular interme- diates integrate in the genome in absence of homologous recombination?. This model postulates that an unex- cised interstrand crosslink could lead to breakage of the sister chromatid, with circularization of a retained frag- ment and integration of the fragment into the genome [23].

Replication origin location might contribute to genetic variability in Trypanosoma cruzi

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Nonethe- less, in the last years, evidence has accumulated suggest- ing that gene arrays are sites that favor homologous recombination (HR) [36–38] as the driver of genetic variability among these families [38].

CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish

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TALEN-mediated precise genome modification by homologous recombination in zebrafish. Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.

Intra-specific comparison of mitochondrial genomes reveals host gene fragment exchange via intron mobility in Tremella fuciformis

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Strand invasion structures in the inverted repeat of Candida albicans mitochondrial DNA reveal a role for homologous recombination in replication. The Agrocybe aegerita mitochondrial genome contains two inverted repeats of the nad4 gene arisen by duplication on both sides of a linear plasmid integration site. Complete mitochondrial genome of the medicinal fungus Ophiocordyceps sinensis.

Impact of PNPase on the transcriptome of Rhodobacter sphaeroides and its cooperation with RNase III and RNase E

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The deletion of the pnp C-terminal KH and S1 domains in Rhodobacter sphaeroides 2.4.1 was carried out by homologous recombination. reverse primer of the up fragment (see underlined bases in primer pnpFragArev). 1 Generation of RNA 3 ′ ends in bacteria and action of PNPase. a Schematic overview of the pnp operon. In the pnp mutant, the KH-S1 domains were deleted and substituted with a gentamicin resistance gene. A stop codon was inserted at the end of the remaining pnp coding region.

Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots

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In addition, the DNA polymerase delta small subunit and replication protein 32 kDa sub- unit showed downregulated in the DNA replication, mis- match repair, and homologous recombination pathway (Table 2).. Cd content distribution and chemical form distribution Most of the heavy metal absorption takes place through root cells, which distribute toxic ions to specific organs, tissues, or organelles.

Differential expression of microRNAs in tomato leaves treated with different light qualities

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KEGG enrichment analysis of the target genes revealed that the zeatin biosynthesis (ko00908), homologous recombination (ko03440), and plant hormone signal transduction (ko04075) pathways were obviously enriched. Zeatin biosynthesis and plant hormone signal transduction both are related to plant hormones, which indicates plant hormones play a very important role in the light response.

Whole-genome resequencing using nextgeneration and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741

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The two T-DNA sequences are exactly the same, in- dicating that the API gene was not present in the expression vector in Agrobacterium before transformation.. indicating that the loss of API gene is not caused by homologous recombination, so it is spec- ulated that the API gene may be lost during the trans- formation of the recombinant plasmid into Agrobacterium. Analysis of the expression levels of genes near the T-DNA insertion sites.

One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

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Background: The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins.

CNCC: An analysis tool to determine genome-wide DNA break end structure at single-nucleotide resolution

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Homologous recombination (HR) is dependent on the template provided by homologous sequences to repair DSBs, and requires extensive resection of the ends on both sides of the DSB, resulting in extensive single stranded 3′ overhangs. 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0.

Mitochondrial genomes of the early land plant lineage liverworts (Marchantiophyta): Conserved genome structure, and ongoing low frequency recombination

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The spacer region of liverwort mt genomes is composed of ORFs, pseudogene fragments, nuclear homologous se- quences, dispersed repeated sequences, SSRs, and other non-coding sequences of unknown origin. Plastid-derived or horizontally transferred DNA sequences are seemingly lacking in liverwort mt genomes (Table 1), as they were in those of mosses [5]. Gener- ally, the total size of the nuclear homologous sequence content in liverworts (average.

Genetic differentiation and intrinsic genomic features explain variation in recombination hotspots among cocoa tree populations

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Example of the window layout for a 10,750 SNP chromosome.. r: Recombination rate. The evolutionary advantage of recombination. https://doi.org/. The fine- scale structure of recombination rate variation in the human genome.. Fine-scale population recombination rates, hotspots, and correlates of recombination in the Medicago truncatula genome. https://doi.org/10.. Fine-scale maps of recombination rates and hotspots in the mouse genome.

Comparative transcriptomic analysis of thermally stressed Arabidopsis thaliana meiotic recombination mutants

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Plasticity of meiotic recombination rates in response to temperature in Arabidopsis . Meiotic recombination gets stressed out:. Pathways to meiotic recombination in Arabidopsis thaliana . A homologue of the yeast HOP1 gene is inactivated in the Arabidopsis meiotic mutant asy1 . Araport11: a complete reannotation of the Arabidopsis thaliana reference genome

Cooperative Recombination of a Quantized High-Density Electron-Hole Plasma

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In addition, changes in the linewidth, carrier density, and magnetic field scaling of the PL spectral features correlate precisely with the onset of random directionality, indicative of cooperative recombination from a high density population of free carriers in a semiconductor environment..