- Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis. - We established cell suspension culture on Panax vietnamensis and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. - Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. - The biomass production and ginsenoside yield were obtained 9.8 g/L DW and 6.81 mg/g DW, respectively. - The effect of initial sucrose concentrations were also investigated in suspension cultures of P. - vietnamensis for biomass and production of ginseng saponin (secondary metabolite). - The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose concentration from 20 to 50 g/L, but an even higher sucrose concentration of 60 g/L seemed to repress the cell growth, further increase of sucrose concentration upto 70 g/L led to a decrease in ginsenoside accumulation and biomass production. - The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM.. - The annual turnover of ginseng in the United States was $98 million with a growth rate of 26% [1]. - explored as potentially more efficient alternatives for the mass production of ginseng and its active components. - on production of ginseng saponin and polysaccharides by suspension cultures of Panax ginseng were observed by [4]. - The results indicated that the specific production (content) of ginseng polysaccharide was not significantly affected by alteration of the N source and the saponin production was relatively higher within the initial N concentration of 5 mM with nitrate alone or a (NO 3. - In this paper, we established cell suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture.. - Extraction and determination of ginsenoside production were determined as reported previously [5,6].. - Effects different strength of MS medium on biomass and ginsenoside production. - Table 1 shows the effects of different strength of MS medium on biomass and ginsenoside production. - The highest biomass production and ginsenoside yield were obtained 9.8 g/L DW and 6.81 mg/g DW, respectively. - High salt strength (2.0) inhibited a cell growth and ginsenoside production accumulation. - Such a phenomenon was also described in provious cultures of Panax ginseng adventitious roots [7].. - Effect of different strength of MS medium on biomass and ginsenoside production MS medium. - Effect of different sucrose concentrations on cell growth and ginsenoside production. - The effect of initial sucrose concentration and 70 g/L) was also investigated in suspension cultures of P.. - The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose concentration from 20 to 50 g/L, but an even higher sucrose concentration of 60 g/L seemed to repress the cell growth. - Further increase of sucrose concentration upto 70 g/L led to a decrease in ginsenoside accumulation and biomass production (Table 2). - [10, 11] found that the optimal concentration of sucrose for cell growth was between 30 and 50 g/L and upto 70 g/L sucrose inhibited cell growth, while the ginsenoside content showed a steady increase with sucrose concentration of upto 60 g/L. - Effect of different sucrose concentrations on cell growth and ginsenoside production Sucrose. - Effect of different nitrogen concentration. - on cell growth and ginsenoside production The effect of the initial nitrogen concentration in the medium for cell growth and metabolite production was studied in P.. - The highest dry weight reached 10.2 g/L at an initial nitrogen concentration of 30 mM. - The highest ginsenoside production was (7.35 mg/g DW) at initial medium nitrogen concentration of 30 mM after 25 days of culture.. - Effect of different nitrogen concentration on cell growth and ginsenoside production Nitrogen. - z Mean separation by DuncanỖs multiple range test at p ≤ 0.05 In cell cultures of P. - reported that the final dry cell weight was relatively low with the low nitrogen concentration. - Maximum cell dry weight obtained (15 g/L) at a total initial nitrogen concentration of 40 mM and the cell growth was inhibited at a high initial nitrogen concentration of 80 mM. - Similarly, the accumulation of total saponin and polysaccharide were also influenced by initial nitrogen concentration in the medium. - maximum production of ginseng saponin and polysaccharide obtained (1.5 g/L and 2.19 g/L) at the initial nitrogen concentration of 40 mM [12]. - In the simultaneous production of ginseng saponin and polysaccharide by suspension cultures of P. - ginseng, [4] reported that production of ginseng saponin was related with the total nitrogen concentration. - The result suggested that a low nitrogen concentration was beneficial for the stimulation of total saponin production.. - Ernst, The efficacy of ginseng: a systematic review of randomised clinical trials, European J. - Zhong, Production of ginseng and its bioactive components in plant cell culture:. - Zhong, Simultaneous production of ginseng saponin and polysaccharide by suspension cultures of Panax ginseng: Nitrogen effects, J. - Paek, The adventitious root cultures of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv), Proceeding of National Conference on Life Sciences, Vietnam, 2007, pp 828-831.. - Yu, Production of the useful metabolites through bioreactor culture of Korean ginseng (P.. - Zhong, Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng production by suspension cultures of P. - Furukawa-Stoffer, Triacylglycerol biosynthesis and gene expression in microspore derived cell suspension cultures of oilseed rape, J. - Park, Characteristics of the growth and ginsenosides in the suspension culture cells of Korean ginseng (P. - Park, Production of ginseng saponin in tissue culture of ginseng (P.. - Wang, Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of P.