- circular RNAs in the brain of nurse and forager honeybees (Apis mellifera). - Results: We identified numerous circular RNAs in the brain of A. - In line with previous findings of circular RNAs in Drosophila, circAmrsmep2 accumulates with increasing age of the insect. - Full list of author information is available at the end of the article. - 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0. - The 5’-end of a donor exon is joined to a 3’-end of an acceptor exon of the same molecule [30]. - An excerpt of the most prominent entities is shown in Table 1.. - A complete listing of the results can be found in Additional file 3.. - The respective chromosome is indicated in the Chr. - The full list can be found in the Additional file 3. - 2 Location of circRNAs in the original transcripts. - Most of the circRNAs in A. - However, we did not find as many BSJ including exons of the 3’-UTR as reported there. - 3 Exon position of circRNAs in the host gene. - Exons of the parent transcripts were numbered. - The number of the first an the last exon included in a circRNA is represented in the left (5’-end) and right (3’-end) panel, respectively. - The most rele- vant regions for circularization are probably the end of the 5’ flanking and the start of the 3’ flanking intron, see Fig. - b BLAST bitscores of the best reverse complementary match of the shorter circRNA flanking intron to the other (yellow) compared to control intron pairs (gray). - Figure 5d shows that the increase in folding-potential (represented by lower MFE scores) is linked to GC- content of the respective introns. - With about 10.4 target sites per 1000 nt circRNAs have a 1.7× increase in conserved, putative miRNA target sites compared to the median of the linear splice product control. - course of the study: circAmrad and circAmrsmep2, respectively. - The expression levels in the SCC experiment (Fig. - 0.001, Students t-test) than in the control. - The number of biological replicates is indicated in the bars. - Either the task of the bee is influencing circAmrad expression or vice versa. - melanogaster, back- splicing is correlated with the length of the 5’ and 3’ flanking introns [35]. - Efficacy of the RNase R treatment was verified in a control experiment shown in Additional file 6. - The number of exons spanned by the transcript was noted for the 5’ and 3’ end of the BSJ. - Flanking introns were determined by including the sequence outside of the BSJ until the next exon in the same transcript.. - Results of the circularity validation through PCR. - Caste and ecology in the social insects. - https://doi.org/10.1007/. - https://doi.org/10.1098/rspb.2000.1345.. - Assessment of pollen stores by foragers in colonies of the honey bee, Apis mellifera l. - https://doi.org/10.1007/s . - https://doi.org/10.1126/science.246.4926.. - The Wisdom of the Hive. - https://doi.org/10.1002/cne.23500.. - https://doi.org/10.. - Responses to sugar and sugar receptor gene expression in different social roles of the honeybee (Apis mellifera). - https://doi.org/10.1016/j.jinsphys.2017.. - https://doi.org a0.. - Experience-expectant plasticity in the mushroom bodies of the honeybee. - https://doi.org/10.1101/lm.5.1.115.. - Development and experience lead to increased volume of subcompartments of the honeybee mushroom body. - https://doi.org/10.1016/. - https://doi.org/10.1002/dneu.22195.. - Experience- and age-related outgrowth of intrinsic neurons in the mushroom bodies of the adult worker honeybee. - https://doi.org . - https://doi.org/10.1002/dneu.22290.. - https://doi.org/10.1186/gb- 2002-3-2-research0007.. - https://doi.org/10.1016/j.jinsphys . - https://doi.org/10.1002/dneu.20929.. - https://doi.org/10.1073/pnas . - Quantitative neuropeptidome analysis reveals neuropeptides are correlated with social behavior regulation of the honeybee workers. - https://doi.org/10.1021/acs.jproteome.. - https://doi.org/. - https://doi.. - org/10.1038/s . - org/10.1021/pr500843j.. - https://doi.org/10.1111/j x.. - https://doi.org/10.1186/gb-2007-8-6-r97.. - https://doi.org/10.1038/nature11928.. - https://doi.org/10.1038/nbt.2890.. - https://doi.org/10.1371/journal.pgen.1003777.. - org/10.1016/j.molcel . - https://doi.org/10.1038/nature11993.. - https://doi.org/10.1016/j.ibmb . - https://doi.org/10.1016/j.celrep . - https://doi.org/10.1101/gad . - https://doi.org/10.1093/nar/gkl151.. - doi.org/10.1074/jbc.M606744200.. - https://doi.org/10.1371/journal.pcbi.1005420.. - https://doi.org/10.1002/. - Single-copy nuclear genes resolve the phylogeny of the holometabolous insects. - doi.org . - https://doi.org/10.1186/s z.. - https://doi.org/10.1242/jeb.053421.. - https://doi.org/10.1101/. - https://doi.org/10.1074/jbc.M511100200.. - https://doi.org/10.1371/. - https://doi.org/10.1038/nn.3218.. - https://doi.org/10.1038/nature10442.. - https://doi.org/10.1016/j.molcel.. - https://doi.org/10.1016/j.bbamem . - doi.org/10.1016/S . - Proceedings of the National Academy of Sciences of the United States of America. - doi.org/10.1073/pnas . - https://doi.org/10.1126/science.1111331.. - https://doi.org/10.2108/zs160043.. - https://doi.org/10.1242/jeb.124859.. - https://doi.org/10.1186/gb-2014-15-2-r34.. - doi.org/10.1093/bioinformatics/btp324.. - https://doi.org/10.1093/bib/bbx014.. - https://doi.org/10.1093/nar/gkw1119.. - https://doi.org/10.1093/bioinformatics/btr167.. - https://doi.org/10.1038/nmeth.1923.. - https://doi.org/10.1093/nar/gkt1181.. - https://doi.org/10.1016/j.cell . - org/10.1093/nar/gkv1208.
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