Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria
- Whole genome sequence based capsular typing and antimicrobial resistance. - prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria. - Background: Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. - Whole genome sequencing has, in recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. - This study characterized vaginal and rectal isolates of Group B Streptococcus obtained from pregnant women in Port Harcourt, Nigeria using a whole-genome sequence-based approach.. - Of the alpha-like proteins (alp) identified, Alp1 was the most prevalent in 11 (33.3. - ermB was detected in 1 (3. - Conclusions: The data available from the whole genome sequencing of these isolates offers a small but insightful description of common serotypes and resistance features within colonizing GBS in Nigeria.. - Keywords: Whole genome sequencing, Group B Streptococcus, Nigeria, capsular typing, Antimicrobial resistance. - To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.. - The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.. - Full list of author information is available at the end of the article. - Streptococcus agalactiae, also known as Group B Streptococcus (GBS) is increasingly being recognized in Africa as one of the major pathogens responsible for neonatal sepsis [1–3]. - Whole genome sequencing (WGS) has emerged as a reliable and cost-effective method for capsular typing and for accurate predictions of minimum inhibitory con- centrations for antimicrobials in GBS [13–15].. - This study analysed whole genome sequences of col- onizing Group B Streptococcus isolates from vaginal and rectal swabs obtained from a previously described study in pregnant women in Port Harcourt, Nigeria [16]. - Details of the study design and sample collec- tion are described previously by Bob-Manuel et al. - The isolates were shipped on Columbia agar with chocolated horse blood (Oxoid, UK) to the Streptococcus Laboratory, Centers for Disease Control and Prevention (CDC), for whole genome sequencing.. - Whole genome sequencing and strain feature prediction At CDC, GBS isolates were cultured on Trypticase soy agar supplemented with 5 % sheep blood prior to DNA extrac- tion from single colonies as previously described [13].. - Whole genome sequencing revealed 3 of the isolates to be non-GBS. - of the ST19 sequence strains were serotype V isolates while serotype III accounted for 1 (11.1. - Two isolates were negative for any of the alpha- protein-like family target predictions.. - of the isolates, while 3 (9.1. - of the iso- lates with resistance rates to erythromycin and clindamy- cin of 30.3 and 24.2. - Table 2 Whole Genome Sequencing predicted antibiotic susceptibility for 33 non-duplicate GBS isolates (See Supplemental Table 1 for MIC prediction values). - Also, all of the isolates with fluoroquinolone resistance deter- minants were serotype V (81.1. - This study reports the molecular characterization of col- onizing strains of Group B Streptococcus in the vagina and rectum of pregnant women. - Serotype V was the most frequent serotype in this population representing 33.3 % of the population.. - Sequence types ST and 2 accounted for 69.7 % of the total isolates. - While ST182 was reported as a frequent clonal type by Medugu et al. - The pubMLST [21] (http://pubmlst.org accessed June 10, 2021) and BacWGSTdb 2.0 [22] (bacdbcn/. - In this study however, clindamycin and erythro- mycin resistance were relatively high at 24.2 and 30.3. - The only tetracycline gene present in this population was tetM. - The presence of the double gyrA + parC mutants conferring full fluoroquinolone-resistance was. - Occurrence of either of these clonal complexes is likely a reflection of the circulating clones in those geo- graphical areas in which they are found.. - This study has demonstrated some salient characteris- tics of the prevalent colonizing strains of GBS in Nigeria, thus, contributing to the body of knowledge concerning GBS, as well as providing AMR data if IAP is considered in the future.. - The online version contains supplementary material available at https://doi.. - org/10.1186/s z.. - The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.. - LM coordinated the whole genome sequencing and analyses. - MB wrote most of the manuscript with major review and editing by LM. - Funding for whole genome sequencing was provided by CDC through Advanced Microbial Detection Project FY21-AMD-161.. - Vertical transmission of group B streptococcus and associated factors among pregnant women: A cross-sectional study, Eastern Ethiopia. - https://doi.org/10.2147/IDR.S150029.. - Medugu N, Iregbu KC, Parker RE, Plemmons J, Singh P, Audu LI, et al. - https://doi.org/1 0.1016/j.cmi . - Sinha A, Russell LB, Tomczyk S, Verani JR, Schrag SJ, Berkley JA, et al. - Disease burden of Group B streptococcus among infants in Sub-Saharan Africa.. - https://doi.org/10.1097/INF.. - Dangor Z, Lala SG, Cutland CL, Koen A, Jose L, Nakwa F, et al. - 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