Transcriptional profiling reveals changes in gene regulation and signaling transduction pathways during temperature stress in wucai (Brassica campestris L.)
- Conversely, high-temperature stress inhibited the expression of pivotal genes and weakened the self-regulating ability of the plants.. - Full list of author information is available at the end of the article Yuan et al. - https://doi.org/10.1186/s . - Elucidating the regulatory pattern of the re- sponse of wucai to LT is thus of great interest. - After 3d of LT treatment, there was no obvious change in the appearance of the seedlings. - The physiological parameters of the wucai seedlings treated under different temperatures showed different trends. - formation rate of the experimental materials under LT were significantly lower than under HT (Fig. - 1 The effects of different temperature stresses on the plant height (a), single plant weight (b), and relative water content (RWC, c) of the leaves of wucai. - Screening was conducted based on the expression level of the DEGs in the different groups. - GO and KEGG analysis of the DEGs of LT and HT. - In the molecular. - KEGG pathway enrichment analyses of the DEGs in the LT-vs-Cont (a). - KEGG pathway enrichment analyses of the DEGs in the HT-vs-Cont (b). - The enriched KEGG pathways of the DEGs differed between the different temperature treatments. - A – C Quantification of the Chl a content (a), Chl b content (b), total Chl content (c). - The effect of different temperature stress treatments on the Chl content of the wucai seedlings. - The F v / F m , F v /F o , and PI abs of the wucai seedlings showed a downward trend during the 3 d of low temperature and high temperature treatments and were reduced on the third day of treatment. - Effects of different temperature stresses on the gas exchange parameters of the wucai seedlings. - On the con- trary, under temperature stress, the C i value of the wucai seedlings increased significantly, the effect of HT was higher that the one of LT (Fig. - The physicochemical properties of the members of the BrLhc superfamily of wucai are shown in the table (Additional file: Table S6). - 10 Cis-acting elements of the BrLhc superfamily. - MEGA7.0 software to conduct a phylogenetic analysis of the BrLhc protein. - The results showed that the expression of the randomly selected differential genes was consistent with the transcrip- tome results (Fig. - Compared with the seedlings treated with high temperature, the seedlings treated with low temperature had stronger water reten- tion ability of the leaves. - Evaluation of the degree of oxidative damage under temperature stress and the mechanism of improved antioxidant capacity. - Temperature stress is one of the primary abiotic stresses experienced by plants during growth [30]. - and MDA following temperature stress, and the MSI decreased significantly, this is similar to the results of the Chakraborty’s study [35]. - Under temperature stress, the P N of the LT seedlings was higher than that of the HT seedlings, which indicated that the seedlings were more sensitive and susceptible to damage under high-temperature stress than under low-temperature stress. - PSII is the most sensitive component of the photo- system. - PsbQ can compensate for the damaged PsbP and cause a series of conformational changes in the Mn clusters of the water oxidation machinery [49]. - Our research showed that compared with the high temperature treat- ment, the F v /F o of the seedlings decreased more slowly in the low temperature environment. - After absorbing light energy, one part of the light energy is transferred along the electron transport chain, and the other part is dissipated in the form of thermal energy. - This is consist- ent with the results of the ROS-related indicators in this study.. - Analysis of the influence of different temperature stresses on the photosynthetic antenna protein pathway. - The regula- tion of the transcriptome confirms the unique protective mechanism of wucai under LT stress. - Bioinformatics analysis of the light-harvesting complex (LHC) family. - org/10.1186/s . - Line ‘ WS-1 ’ was screened by the corresponding author and all seed materials were kept in the Vegetable Breeding Laboratory of the College of. - 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