Whole-genome sequencing of Aspergillus tubingensis G131 and overview of its secondary metabolism potential

- Whole-genome sequencing of Aspergillus tubingensis G131 and overview of its.
- tubingensis G131.
- tubingensis G131..
- tubingensis, which is part of the A..
- A complete review of the putative SM gene biosynthetic clusters is also reported..
- tubingensis G131 produces asperazine and NGPs under laboratory condi- tions.
- tubingensis G131 genome is robust.
- tubingensis G131 (35,18 Mb) is equivalent to the genome size of A.
- Table 1 Genome characteristics and predicted features of the assembled A.
- Length of the largest scaffolds 2,380,764 bp 4,803,603 bp.
- also part of the A.
- tubingensis strain is, most of the time, misidentified as A.
- Samson et al.
- tubingensis G131 on CYA plate after 7-day-culture at 28 °C.
- tubingensis G131 conidia and spores after 7-day-culture at 28 °C (Hitachi TM-3000, Bench SEM).
- tubingensis G131 could be linked to the prediction methodology.
- tubingensis G131) for proteins prediction in A..
- Venn diagram of the OrthoMCL results (Fig.
- 3b) shows a strong homology between the four strains of the A.
- tubingensis G131 were not sorted in orthologous clusters and were specific of the studied strain.
- Focus on Aspergillus tubingensis G131 secondary metabolism General observation on secondary metabolites biosynthesis Regarding dot-plot analysis of A.
- tubingensis G131 against A.
- tubingensis G131 was done using two available software packages: AntiSMASH and SMURF [38, 39].
- Inglis et al.
- tubingensis G131 putative SM clusters and their genomic coordinates are available in Additional file 1: Table S4..
- tubingensis G131 are defined according to their “backbone enzymes” that gen- erate the carbon skeleton of the putative SM.
- tubingensis G131, most of the “ backbone enzymes ” are polyketide synthase (PKS) or non-ribosomal peptides synthase (NRPS).
- tubingensis G131 possesses a greater number of PKS / NRPS hybrid SM clusters (13) than the other strains (Table 2)..
- tubingensis G131 SM clusters were individually blasted against A.
- tubingensis CBS 134.48 (de Vries et al., 2017).
- (Andersen et al., 2011).
- (Yamada et al., 2016).
- tubingensis G131 with genome assembly of A.
- tubingensis G131 (7 SM clusters)..
- tubingensis G131 could produce TAN-1612, a neuropeptide Y antagonist, already shown to be produced by A.
- tubingensis G131 could produce anthra- cenone / naphthacenedione compounds with potential immunosuppressive properties..
- tubingensis G131 Through biochemical analyses, Bouras et al.
- tubingensis G131 does not produce OTA under certain conditions.
- Cabañes et al.
- In addition, a 21-kb region of the A.
- tubingensis G131 genome (NCBI ac- cession number and blast results are available in Add- itional file 1: Table S5).
- tubingensis G131 does not contain an orthologue of An15g07920..
- tubingensis G131 could not produce OTA.
- In addition, one of the predicted proteins of A.
- tubingensis G131 In contrast with OTA produc- tion, both A.
- Susca et al.
- tubingensis G131 (Additional file 1: Table S5)..
- tubingensis G131 could not produce fumonisins..
- tubingensis G131 grown on CYA plate at 28 °C (Table 4).
- Putative SM cluster for asperazine biosynthesis Asperazine, a diketopiperazine of the ditryptophenaline family (Fig.
- According to Nielsen et al.
- tubingensis G131 on CYA plate at 28 °C.
- niger clade (Nielsen et al.
- However, none of the two cytochromes P450 present in the SM cluster 46 shows homology with DptC.
- Kishimoto et al.
- tubingensis G131 – a.
- Schematic representation of the putative asperazine SM cluster (cluster 46).
- tubingensis G131 - a.
- Schematic representation of the putative NGP SM Cluster (Cluster 16).
- Schematic representation of the putative NGP SM cluster (cluster 16).
- tubingensis G131 and highlight its secondary metabolism potential.
- tubingensis G131 is not able to produce these two types of mycotoxins.
- tubingensis G131 was isolated from a French Mediter- ranean vineyard, classified into Aspergillus section Nigri (morphological characterization) and described as non- ochratoxigenic (biochemical characterization) [25].
- tubingensis G131 and A..
- tubingensis G131 scaffolds assembly.
- tubingensis G131 and A.
- SM Clusters coordinates of the scaffolds assembly.
- tubingensis G131 and others known fungal biosynthetic cluster.
- AntiSMASH schematic representation of the conserved domains in SM clusters unique in A..
- tubingensis G131 and their similarities with other SM clusters identified in various fungi (AntiSMASH results).
- tubingensis G131 putatively involved in NGPs synthesis.
- tubingensis G131 with other known fungal genomes assembly..
- JR and FM made a critical revision of the manuscript.
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