Tìm thấy 18+ kết quả cho từ khóa "Confocal microscopy"
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Laser confocal microscopy revealed that amyloid-beta was internalized by the cells (Figure 2).. At the end of the incubation, cells were lysed as was mentioned above and Sirt1, total, and phosphorylated forms of GSK-3α/β and Akt were visualized and quantified..
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D Confocal microscopy images of cell internalization testing of Exosome-DSPE-PEG-AbCD34 at ×20 magnification. 3 Synthesis of Exosome-DSPE-PEG-AbCD34-Smad7 and its effect on SMAD7 expression in MAEC cells. A DNA gel electrophoresis was used to detect the loading of pcDNA3.1-Smad7 in the exosome, Exosome-DSPE-PEG and Exosome-DSPE-PEG-AbCD34.
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The cells were supplemented with only P in the growth medium for 30 min at 37 ◦ C, which led to no fluorescence as determined by laser scanning confocal microscopy (ex = 405 nm) (Figure 6a). that probe P can penetrate the cell membrane and might be used for detecting Al 3+ in living cells.. 2-Hydroxy-1-naphthaldehyde (1.0 mmol) and benzoichydrazide (1.0 mmol) were mixed and stirred in ethanol (30 mL) at 80 ◦ C for 4 h and then cooled to room temperature.
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Detection of the expression of YFP in milk from SNU-SB-1 by confocal microscopy. 5 ′ junction sequence analysis of all integration sites in SNU-F1 – 1. Sequences showing the genome-to- transposon junctions in the genome of SNU-F1 – 1 and the integration of transgenes at TTAA sites. All transgene integration sites in SNU-F1 – 2..
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Membrane expression of the protein was examined using confocal microscopy (Fig. 2C and the gerbil prestin on the right were quite similar despite their differences in the amino acid sequences. Using the first derivative of the Boltzmann function, four parameters (Q max , C lin , V 1/2 , and z) from nonlinear curve-fitting of the NLC were calculated.. Gaps in the aligned sequences were indicated by the dashed line.
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Multi-photon absorption pro- cess has been studying on the fields of laser scan confocal microscopy [14], optical power limiting [15], multi-photon microscopy [16, 17], micro-fabrication [18], upcon- version lasing [19], photodynamic therapy [20], and multi-photon luminescence [21].. In an our previous paper [22], we suggested a method for obtaining the explicit ex- pression of absorption power in the presence of magnetic field with multi-photon ab- sorption process.
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Three-Dimensional Confocal Microscopy of the Living Human Cornea Barry R. Volume II of the Handbook of Optics is a continuation of Volume I. There are now two different uses of the word emissivity. and a subscript v indicates a quantity normalized to the response of the eye. k B Boltzmann constant JK –1 ] m e mass of the electron kg]. Bessel function of the first kind. n real part of the relative refractive index. The size of the image can be determined by trac- ing a second, “principal (chief.
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Three-Dimensional Confocal Microscopy of the Living Human Cornea Barry R. There are now two different uses of the word emissivity. and a subscript v indicates a quantity normalized to the response of the eye. k B Boltzmann constant JK –1 ] m e mass of the electron kg]. Bessel function of the first kind. n real part of the relative refractive index. Then an operational definition of the spectral radiance is. θ φ and spectral (l) structure of the light field.
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It took 5 to 12 weeks for the RLD (reduced number of long days) ecotype to regenerate in 1998 (Sikdar et al., 1998). gfp, encoded in the second ORF, is expressed only in chloroplasts, allowing confocal microscopy to rapidly classify transplastomic clones in a limited number of heteroplasmic cells (Yu et al., 2017). Besides, the recovery of homoplasmic plant lines is another big obstacle for this technology (Ahmad et al., 2016).
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To further demonstrate the practical applicability of the probe P, confocal microscopy experiments were further carried out, and the fluorescence images of HepG2 cells were recorded before and after the addition of Cu 2+. The bright field images of Figure 10a and Figure 10b were shown as Figure 10c and Figure 10d, and the shapes of cells indicated that P has low toxicity. Cu 2+ could induce spirolactam ring opening of the rhodamine unit and achieved an “off–on”.
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When the cord blood T cells were exposed to 100 μg/. 1 Characterization of cord blood naive T cells. b Transmission electron microscopy (TEM) of K562-derived exosomes, c Western blot analysis of the purified exosomes obtained using. d Size analysis of K562- derived exosomes using dynamic light scattering (DLS). 3 Confocal microscopy confirmed uptake and internalization of K562-derived exosomes (labeled with the PKH26 lipophilic dye) by recipient cells with nucleus staining by DAPI. a Uptake of K562
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The transformed protoplasts were incubated in the dark for 14 h and then visualized using confocal microscopy. b Immunogold labeling of VvSNAT2 using ultrathin sections of grapevine leaves.. The K m value of VvSNAT2 was similar to that of OsSNAT2 (372 μM) but different from that of OsSNAT1 (270 μM) [8, 9].. However, the V max value of VvSNAT2 was much lower than those of OsSNAT1 and OsSNAT2 (3.3 and 4.7 nmol/min/mg protein, respectively) [8, 9].
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As 5Me- dC deamination results in thymidine, which is proc- essed by mismatch repair mechanisms far less efficient than one involving uracil removal by UNG, 5Me-dC de- amination by mouse A1 could contribute to the numer- ous 5MeCpG deamination hotspots evidenced in many genes associated with cancer [39, 71]. a Western blot analysis of V5-tagged mouse APOBEC cytidine deaminases in quail QT6 cells. b Confocal microscopy analysis of V5-tagged mouse APOBEC cytidine deaminases in QT6 cells, 24 h post
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To test our prediction, we examined the germlines of N2 and AB1 adults in the different growth conditions via confocal microscopy (Fig. a N2 and AB1 germlines exhibit normal germ cell morphologies and numbers in OP50 NGM (aberrant germlines were observed in 21 and 12.5% of the worms, respectively). However, embryos accumulate in 36% of the S-Medium grown adults. More pro- nounced cuticular structures were detected in the earlier larval stages (L3) with SEM.
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KÍNH HIỂN VI QUANG HỌC (Light microscopy) Components and light path of a bright-field microscope Junqueira’s basic histology (text & atlas), 13th edition, Anthony L. Appearance of cells with fluorescent microscopy - N: cell nuclei - R: RNA-rich cytoplasm Junqueira’s basic histology (text & atlas), 13th edition, Anthony L. Principle of confocal microscopy Junqueira’s basic histology (text & atlas), 13th edition, Anthony L.
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The cell viability of SGC-7901/5-FU cells after treated with 5-FU (a) or CDDP (b) with or without the exosomes isolated from SGC-7901 or MGC-803 cells for 48 or 24 h, respectively. 4 Exosomes derived from sensitive GC cells were taken up by SGC-7901/5-FU cells. a Confocal microscopy showed exosome internalization by SGC-7901/5-FU recipient cells after co-incubation with PKH26-labeled SGC-7901 exosomes. b The MGC-803-pLVX stable cells were placed in the upper chamber and coincubated with SGC-7901/
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Meanwhile, confocal microscopy observation showed that the intensity of green fluorescence signal Table 3 Absolute quantitative analysis and their expression of the BBTV genomic components of B2 and B4 samples. There- fore, BBTV individual genomic gene transcription was regulated by the promoter sequence in the intergenic re- gion of each component, and a cis-acting element from DNA-N component had a high transcriptional activity for gene transcription..
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The results were normalized to the expression level of the constitutively expressed actin gene [57]. tumefaciens C58 were allowed to grow at 23 °C in the dark for 24 h, followed by another 24 h under a 16 h-light: 8 h-dark photoperiod before the leaves were detached for obser- vation of the GFP signal using confocal microscopy.. tumefaciens suspension removed with sterile filter paper and inoculated into MS media for cocultivation in the dark for 48 h.
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Confocal microscopy revealed fluorescence from the SbER2 – 1-eGFP fusion protein on the cell mem- brane and chloroplasts of Arabidopsis protoplasts, while eGFP (expressed from the control construct 35S::eGFP) was dispersed throughout the whole cell (Fig. These observa- tions indicated that the SbER2 – 1-eGFP fusion protein was localized on the cell membrane and chloroplast, which was inconsistent with the location in the plasma membrane pre- dicted using CELLO v2.5 software.
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Confocal microscopy demonstrated that EPA reduced red JC-1 fluorescence and increased green JC-1 fluorescence, leading to a reduction in the red/green fluorescence ratio. The fluorescence intensity gradually decreased with the progressive increase of EPA concentration and mitochondrial membrane po- tential in MM.1R cells drop to the maximum extent in the combined treatment group of EPA + dexa- methasone(P <.