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DNA synthesis

Tìm thấy 12+ kết quả cho từ khóa "DNA synthesis"

DNA evidence is a frozen moment in time

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The PCR Process - Mechanism of DNA Synthesis. DNA polymerase extends the primer by sequentially adding a single dNTP (dATP, dGTP, dCTP or dTTP) that is complementary to the existing DNA strand. The sequence of the newly synthesized strand is complementary to that of the template strand.. The dNTP is added to the 3´ end of the growing DNA strand, so DNA synthesis occurs in the 5´ to 3´ direction..

PCR

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The PCR Process - Mechanism of DNA Synthesis. DNA polymerase extends the primer by sequentially adding a single dNTP (dATP, dGTP, dCTP or dTTP) that is complementary to the existing DNA strand. The sequence of the newly synthesized strand is complementary to that of the template strand.. The dNTP is added to the 3´ end of the growing DNA strand, so DNA synthesis occurs in the 5´ to 3´ direction..

Chapter 062. Principles of Human Genetics (Part 4)

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After traversing a critical checkpoint in G 1 , cells undergo DNA synthesis (S phase), during which the DNA in each chromosome is replicated, yielding two pairs of sister chromatids (2n →4n). The process of DNA synthesis requires stringent fidelity in order to avoid transmitting errors to subsequent generations of cells.

NanoMod: A computational tool to detect DNA modifications using Nanopore longread sequencing data

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Similarly, incorporation of modified DNA bases during DNA synthesis can be used to track patterns of DNA replication [25, 26], so accurate detection of de novo DNA modifications on newly synthesized DNA strands enables genome-wide studies on DNA replication timing and patterns.. We have developed a new computational tool, NanoMod, for the detection of DNA modifications using Nanopore long-read sequencing data.

Chapter 081. Principles of Cancer Treatment (Part 15)

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Asparaginase is a bacterial enzyme that causes breakdown of extracellular asparagine required for protein synthesis in certain leukemic cells. This effectively stops tumor cell DNA synthesis, as DNA synthesis requires concurrent protein synthesis. The outcome of asparaginase action is therefore very similar to the result of the small-molecule antimetabolites.

Chapter 104. Acute and Chronic Myeloid Leukemia (Part 7)

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When given in high doses, more cytarabine may enter the cells, saturate the cytarabine-inactivating enzymes, and increase the intracellular levels of 1-β-D-arabinofuranylcytosine-triphosphate, the active metabolite incorporated into DNA. Thus, higher doses of cytarabine may increase the inhibition of DNA synthesis and thereby overcome resistance to standard-dose cytarabine.

Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan–carbon nanotube composite modified electrodes

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of DNA synthesis, there have been some studies on the electrochemical monitoring of MC–DNA interaction.

The whole transcriptome effects of the PPARα agonist fenofibrate on livers of hepatocyte humanized mice

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According to gene set enrichment analysis, fenofibrate upregulated interferon/cytokine signaling-related pathways in hepatocyte humanized liver, but downregulated these pathways in normal mouse liver. downregulated pathways related to DNA synthesis in hepatocyte humanized liver but not in normal mouse liver.. Keywords: Hepatocyte humanized mice, Fenofibrate, PPAR α , Transcriptomics, Lipid metabolism, DNA synthesis. to replacement of the host hepatocytes at a repopulation rate exceeding .

Chapter 100. Megaloblastic Anemias (Part 3)

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The role of folates in DNA synthesis and in formation on S- adenosylmethionine (SAM), which is involved in numerous methylation reactions.. During thymidylate synthesis, 5,10-methylene-THF is oxidized to DHF (dihydrofolate). The enzyme DHF reductase converts this to THF.

The effect of some factors on stimulating the growth and production of active substances in Zingiber officinale callus cultures

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The mevalonate effect for this phase of DNA synthesis suggests that mevalonate, or a derivative, may be required for the activation of DNA polymerase, in all the like- lihood of a-DNA polymerase, which functions specifically dur- ing the S phase of the cell cycle to effect the replication of the DNA chain (Craig et al., 1975.. Keir et al., 1977).

Biochemistry, 4th Edition P88

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The ratio- nale for these varying affinities is as follows (Figure 26.23): High [ATP] is consis- tent with cell growth and division and, consequently, the need for DNA synthesis.. Thus, ATP binds in the overall activity site of ribonucleotide reductase, turning it on and promoting production of dNTPs for DNA synthesis. Upon dGTP association with the substrate specificity site, ADP is the favored substrate, leading to ADP reduction and the eventual accumulation of dATP.

Biochemistry, 4th Edition P87

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Pathways presented thus far in this chapter account for the synthesis of the four principal ribonucleotides: ATP, GTP, UTP, and CTP. Roughly 90% of the total nucleic acid in cells is RNA, with the remainder being deoxyribonucleic acid (DNA). 26.7 How Do Cells Form the Deoxyribonucleotides That Are Necessary for DNA Synthesis?. Reduction at the 2-position of the ri- bose ring in NDPs produces 2-deoxy forms of these nucleotides (Figure 26.19)..

Synthesis of quaternized zinc(II) and cobalt(II) phthalocyanines bearing pyridine-2-yl-ethynyl groups and their DNA binding properties

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The general route for the synthesis of quaternized metallophthalocyanines (5, 6). Determination of binding of Q-ZnPc and Q-CoPc to DNA using UV-Vis titrations. All titrations of Pcs with CT-DNA were performed at room temperature in the buffer solution.

Pyridine substituted BODIPYs: synthesis, characterization and cholinesterease, α-glucosidase inhibitory, DNA hydrolytic cleavage effects

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DNA hydrolytic cleavage properties of the compounds. The DNA hydrolytic cleavage actions of the compounds on supercoiled pBR322 plasmid DNA were determined according to our previously reported methods, and the intensity bands were observed under UV illuminator [32]. To investigate the ability of the compounds to damage the phosphodiester bonds of supercoiled plasmid DNA, we designed hydrolytic. Dixon plot of BDPY-4 on AChE.. The inhibitory type and K i value of the BDPY-4 on AChE..

Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and γ-rays in Arabidopsis: Implication of the presence of a translesion synthesis mechanism in plants

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Burgers, O.M.J., Eukaryotic DNA polymerases in DNA replication and DNA repair (1998) Chromosoma, 107, pp. 218-227 DiGiuseppe, J.A., Dresler, S.L., Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: Mediation of long- patch and short-patch repair by distinct DNA polymerases (1989) Biochemistry, 28, pp.

Electrochemical synthesis of polypyrrole nanowires and application of biosensor

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Potentiostat curve of the synthesis of PPy on Nikel electrode and ITO electrode. Figure 1.10 The schematic of a biosensor. Figure 1.11. General DNA sensor design based on CPs. Figure 1.12. The principle of DNA sensor. Figure 1.13. Figure 1.14. Four base types of DNA. Figure 1.15. Hydrogen bonds between the A-T and G-C bases of the two trands of DNA Figure 2.1. Schematic of electrochemical synthesis system of polypyrrole Figure 2.2. The wave form of the Lock-in Amplifier SR830 Figure 2.5.

Lecture Principles of Biology - Chapter 16: DNA – The genetic material

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DNA replication begins at specific sites. there is usually only one origin of replication in the circular bacterial DNA. both strands are replicated at the same time on both sides of the replication bubble, producing Y-shaped replication forks that move as synthesis proceeds. the DNA molecule). synthesis proceeds by adding nucleotides onto the 3’ end of a strand. thus, synthesis can only proceed in the 5. phosphates are released in the process – energy to drive the reaction comes from these.

Synthesis and biological evaluation of quinoline-triazole and quinolone-triazole conjugates

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In summary, we have described the design and synthesis of novel hybrid compounds between a heteroaryl ring (quinoline and quinolone) and a triazole scaffold. Propar- gyl skeletons are reacted with aromatic and benzylic halides and sodium azide via a CuI/L-proline-catalyzed one-pot synthesis method, and novel quinoline-triazoles 1a–1c and 2a–2c and quinolone-triazoles 3a–3c are. Lane C, control, CT-DNA. Lane 1, CT-DNA + 500 µ g/mL of 1a. Lane 2, CT-DNA + 500 µg/mL of 1b.

Novel “turn off-on” sensors for detection of DNA-acrylamide interaction using ZnS quantum dots as a phosphorescent probe

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The obtained results from the interaction mechanism of ACR and DNA would help to understand the binding of other potential cancer molecules to DNA.. Synthesis of the Mn-doped ZnS QDs. Using 1 M NaOH, the pH of the solution was adjusted to 11.0. In order to prepare the stock ACR solution, 5 mg of ACR was weighed accurately and dissolved in 10 mL of deionized water. Working solutions of ACR were prepared by dilution of the appropriate quantity in buffer solution..

Targeting human telomeric DNA with azacyanines

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Benzimidazole is considered an important scaffold in the design and synthesis of pharmacophores and thus is commonly exploited [20–23]. Several studies investigating the effect of the benzimidazole scaffold on telomeric DNA and oncogene promoters such as KRAS and VEGFR that are known to fold into quadruplex structures were recently reported [24–30].