« Home « Kết quả tìm kiếm

Fluorescence in situ hybridization

Tìm thấy 18+ kết quả cho từ khóa "Fluorescence in situ hybridization"

Chromosome map of the Siamese cobra: Did partial synteny of sex chromosomes in the amniote represent “a hypothetical ancestral super-sex chromosome” or random distribution

tailieu.vn

Application of fluorescence in situ hybridization in genome analysis of the mouse. Karyotypic analysis and FISH mapping of microsatellite motifs reveal highly differentiated XX/XY sex chromosomes in the pink-tailed worm-lizard (Aprasia parapulchella, Pygopodidae, Squamata). Differentiation of sex chromosomes and karyotype characterisation in the dragonsnake Xenodermus javanicus (Squamata: Xenodermatidae).

Chapter 063. Chromosome Disorders (Part 10)

tailieu.vn

Jiang F, Katz RL: Use of interphase fluorescence in situ hybridization as a powerful diagnostic tool in cytology. Lee C et al: Multicolor fluorescence in situ hybridization in clinical cytogenetic diagnostics. Menten B et al: Emerging patterns of cryptic chromosomal imbalance in patients with idiopathic mental retardation and multiple congenital anomalies: A new series of 140 patients and review of published reports. J Med Genet PMID: 16490798].

TRONG CHĂN NUÔI L N TÓM L C: m i ngày). FluorescenceIn Situ Hybridization và Polymerase Chain Reaction khu ch đ i

www.academia.edu

Trong sinh học phân tử, tiến bộ mới chẳng hạn như ứng dụng rộng rãi huỳnh quang In Situ Hybridization (FISH) và kỹ thuật Polymerase Chain Reaction (PCR), đã tạo cơ hội chưa từng có để đạt được hiểu biết về các cấu trúc cộng đồng vi khuẩn ở các giai đoạn khác nhau của điều trị.Song song với việc phân tích vi sinh vật truyền thống, những kỹ thuật này cung cấp triển vọng đầy hứa hẹn để cải thiện quá trình thiết kế và kiểm soát hoạt động.

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alphamethyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

tailieu.vn

Co-localization of zbtb40 and cyp17a1 in ovary and testis Given the similar expression patterns of zbtb40 and cyp17a1 during spermatogenesis, the cellular co-localization of two genes in the gonad was examined in ovary and testis by fluorescence in situ hybridization (FISH, Fig. In ovary, cyp17a1 signals were clearly observed in the cytoplasm of oocytes where no zbtb40 signal was found (Fig.

Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

tailieu.vn

Therefore, generating chromosome-specific molecular markers is critical for detecting and tracing alien chromosomes in wheat – tetraploid Th. Tetraploid Th. elongatum is an important genetic re- source for improving wheat, and some wheat – tetraploid Th. [18] produced a fluorescence in situ hybridization (FISH) karyotype of the E-genome chro- mosomes of tetraploid Th. elongatum based on various repetitive sequence probes, which may enhance the util- ity of tetraploid Th.

Chapter 080. Cancer Cell Biology and Angiogenesis (Part 6)

tailieu.vn

Frequently, patients with mutated alleles also had evidence of EGFR gene amplification by fluorescence in situ hybridization (FISH). In fact, mutated K-Ras, which occurs to the exclusion of EGFR mutation, appears to define a subset of patients with low likelihood of response to EGFR inhibitors. The wild-type EGFR is expressed by many other human cancers, and in colon cancer and head and neck cancers, targeting of the EGFR with a.

Chapter 079. Cancer Genetics (Part 7)

tailieu.vn

This increase in DNA copy number may cause cytologically recognizable chromosome alterations referred to as homogeneous staining regions (HSRs), if integrated within chromosomes, or double minutes (dmins), if extrachromosomal in nature. The recognition of DNA amplification is accomplished through various cytogenetic techniques such as comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH), which allow the visualization of chromosomal.

Hepatocellular Carcinoma: Targeted Therapy and Multidisciplinary P7

tailieu.vn

Wilkens L, Bredt M, Flemming P, Mengel M, Becker T, Klempnauer J, Kreipe H (2002) Comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) in the diagnosis of hepatocellular carcinoma. Chen ZM, Crone KG, Watson MA, Pfeifer JD, Wang HL (2005) Identification of a unique gene expression signature that differentiates hepatocellular adenoma from well-differentiated hepatocellular carcinoma. A marker of hepatocellular differentiation.

Các phương pháp lập bản đồ di truyền người

tailieu.vn

(fluorescence in situ hybridization) sử dụng mẫu dò huỳnh quang thay cho mẫu dò. phóng xạ là nhanh hơn và cho phép phân tích vùng của mẫu dò tốt hơn, thường là ở trong khoảng 1 Mb đối với nhiễm sắc thể kỳ giữa. Bằng cách sử dụng nhiễm sắc thể kỳ trung gian, các nhiễm sắc thể ở kỳ này kết xoắn ít hơn các nhiễm sắc thể kỳ giữa, phân tích FISH có thể được tăng lên từ 25-250 kb.. Lai tế bào soma.

Chapter 104. Acute and Chronic Myeloid Leukemia (Part 6)

tailieu.vn

For patients in morphologic CR, reverse transcriptase polymerase chain reaction (RT-PCR) to detect AML-associated molecular abnormalities and either metaphase cytogenetics or interphase cytogenetics by fluorescence in situ hybridization (FISH) to detect AML-. associated cytogenetic aberrations are currently used to detect residual disease..

Pseudocohnilembus persalinus genome database - the first genome database of facultative scuticociliatosis pathogens

tailieu.vn

Genome of the facultative scuticociliatosis pathogen Pseudocohnilembus persalinus provides insight into its virulence through horizontal gene transfer. accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Stomatogenesis and morphological redescription of Pseudocohnilembus persalinus (Ciliophora:. Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization.

Chapter 108. Hematopoietic Cell Transplantation (Part 3)

tailieu.vn

Following allogeneic transplantation, engraftment can be documented using fluorescence in situ hybridization of sex chromosomes if donor and recipient are sex-mismatched, HLA-typing if HLA-mismatched, or restriction fragment length polymorphism analysis if sex- and HLA-matched.

Chapter 103. Polycythemia Vera and Other Myeloproliferative Diseases (Part 9)

tailieu.vn

Because the bcr-abl translocation can be. present in the absence of the Ph chromosome, and because bcr-abl RT-PCR is associated with false-positive results, fluorescence in situ hybridization (FISH) analysis for bcr-abl is the preferred assay in patients with thrombocytosis in whom a cytogenetic study for the Ph chromosome is negative.

Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.)

tailieu.vn

Among the 404 rye chromosome arm-specific markers . 1 Genomic in situ hybridization (GISH) and non-denaturing fluorescence in situ hybridization (ND-FISH) analyses of 3RS ditelosomic addition line of ‘ Holdfast-KingII ’ and 1R disomic addition line of ‘ Chinese Spring-Imperial. b ND-FISH analysis of the same metaphase cell after GISH analysis (a) with Oligo-pSc119.2 – 1 (green) and Oligo-pTa535 – 2 (red). c GISH analysis of 1R disomic addition line of ‘ Chinese Spring-Imperial.

Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

tailieu.vn

The use of double fluorescence in situ hybridization to physically map the positions of 5S rDNA genes in relation to the chromosomal location of 18S-5.8S-26S rDNA and a C genome specific DNA sequence in the genus Avena. Genetic regulation of diploid-like chromosome pairing in the hexaploid species, Festuca arundinacea Schreb. Genome balance in six successive generations of the allotetraploid Festuca pratensis × Lolium perenne. Evolution of the U-genome cluster.

Demographic, tumour, and treatment characteristics of female patients with breast cancer in Sri Lanka; results from a hospital-based cancer registry

tailieu.vn

Patterns of cancer care in Sri Lanka: Assessing care provision and unmet needs through an electronic database. Pathological prognostic factors in breast cancer. The value of histological grade in breast cancer: experience from a large study with long-term follow-up. Detection and quantitation of HER-2/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization. https://doi.org .

Identification of high-copy number long terminal repeat retrotransposons and their expansion in Phalaenopsis orchids

tailieu.vn

This suggests that some predicted gene do harbor the Orchid-rt1 region.. 3 Fluorescence in situ hybridization (FISH) mapped the LTR sequence of Orchid-rt1 on chromosomes of P. a, c, e and g The distribution of Orchid-rt1 (red) on chromosomes (blue). qRT-PCR was applied to validate the expres- sion level of the predicted Orchid-rt1 inserted genes and its paralogous genes (Fig.

Enhanced sugar accumulation and regulated plant hormone signalling genes contribute to cold tolerance in hypoploid Saccharum spontaneum

tailieu.vn

Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods. The importance of the wild cane Saccharum spontaneum for bioenergy genetic breeding. A BAC library of the SP80 – 3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome.. Overexpression of the transcription factor AP37 in Rice improves grain yield under drought Conditions 1[W.

Satellite DNA in Paphiopedilum subgenus Parvisepalum as revealed by highthroughput sequencing and fluorescent in situ hybridization

tailieu.vn

Details of the origin of plant materials used for Illumina sequencing, fluorescent in situ hybridization (FISH), and genome size estimations and the location of the voucher specimens are given in Additional file 7: Table S3. All other nrITS sequences used in the phylogenetic analysis were downloaded from GenBank.. 1 cm 2 of leaf material of the Paphiopedilum sample was co-chopped with the ref- erence standard Vicia faba ‘Inovec’ (2C = 26.9 pg, [59]).

XỬ LÝ NƯỚC THẢI CÓ NỒNG ĐỘ CHẤT HỮU CƠ CAO TRONG ĐIỀU KIỆN HIẾU KHÍ ƯA NHIỆT

tailieu.vn

Sự thay đổi và mối tương quan của các nhóm vi sinh vật trong thiết bị phản ứng được làm sáng tỏ nhờ áp dụng các kỹ thuật cao như PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis) và FISH (Fluorescence In-Situ Hybridization), từ đó tìm ra mối tương quan giữa các thông số hóa lý với sự thay đổi trong cấu trúc của cộng đồng vi sinh vật..