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Microplate reader

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A simple automated microplate method for determining reducing sugars in food extracts and synthetic serum using cupric-neocuproine as reductant

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Proposed microplate Cu(II)-Nc method. The alkaline Cu(II)-Nc spectrophotometric method to assess the total reducing sugar content of food extracts and synthetic serum was adapted with some modifications to the 96-well microplate format. The microplate system contained two dispensers. The experimental set-up of absorbance measurement in the microplate reader system is shown in Figure 4.. Schematic view of the instrumentation used for absorbance measurements in the microplate reader system..

Pyridine substituted BODIPYs: synthesis, characterization and cholinesterease, α-glucosidase inhibitory, DNA hydrolytic cleavage effects

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The inhibitory properties of the enzymes were carried out using Thermo Scientific Multiskan TM Go Microplate Spectrophotometer using a 96-well microplate reader. AChE and BuChE inhibition of the compounds were measured according to Ingkaninan’s method with some modifications using a 96-well microplate reader [1].. Tris-HCl buffer pH 8 (50 µL, 50 mM), DTNB (125 µL, 3 mM), AChE/BuChE (25 µL, 0.2 U/mL) and the compounds were added in the microplate and incubated for 15 min at.

Cobalt chloride alters mitochondrial function of in vitro cultured cardiomyocytes in a dose-dependent manner

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The absorbance value indicating cell viability was measured at 450 nm using a microplate reader. The number of alive cells in each well was expressed as a value relative to the normal control.. H9C2 cells were seeded at a density of 10 4 cells/well in 96-well black, glass bottom plates (CAT. After treatment, the cells were stained with either 0.1 µM NAO (ex/em: 495/519 nm, Invitrogen, USA) or 1 µM TMRE (ex/em: 535/570 nm) for 30 min at room temperature.

Protein Methods (Revised)

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Reader SpectraMax L Microplate ReaderELISAs and immunoassays Aequorin assaysNucleic acid (DNA) quantitation ATP detectionProtein quantitation Bioluminescence resonance energy transfer (BRET)Reporter gene assays Cell viability, proliferation, and cytotoxicityCell Viability, proliferation, and cytotoxicity ELISAs and immunoassaysEnzyme assays Enzyme assaysTransporter assays Mycoplasma detectionPhosphotases/kinases Reporter gene assaysMicrobial growthELISA (4)Multiwash+ AquaMaxMáy Multiwash+ rửa đĩa

Knockdown of circSMAD2 inhibits the tumorigenesis of gallbladder cancer through binding with eIF4A3

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Finally, the absorbance of GBC cells was measured at 450 nm using a microplate reader (Thermo Fisher Scientific).. Lastly, the enrich- ment of circSMAD2 or SMAD2 was tested using RT- qPCR. Then, GBC cells (1 × 10 6 cells/ml) were seeded into the upper chambers (the medium did not contain FBS). GBC cells or tissues were isolated from cells by RIPA buffer and quantified by BCA kit (Beyotime). Characteristics Cases Low expression of circSMAD2.

Isolation, screening, and partial characterization of oxalate oxidase enzyme from pseudomonas strain under induced oxalate stress condition

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The reading was noted on SYNERGY H1 microplate reader Biotek ® at 600 nm (Kanauchi et al., 2009 . Hence 3% concentration of PO is selected in growth medium or is found to be more suitable for bulk production of oxalate enzyme.. 10 0.125. Characterization of oxalate oxidase enzyme Ammonium sulphate fractionation and dialysis:. Neut et al., 2005)..

Investigation of cytotoxic activity of cyanobacterial strains isolated from water and soil samples collected in Hanoi

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After 48 hours, cells were dyed with MTT, and the optical density was measured using a Microplate reader SpectraMax Plus384 (GMI, USA) at two wavelengths of 570 nm and 690 nm [15]. The isolation process based on the different morphology features of cyanobacterial colonies led to the establishment of six monoculture strains.

Silk fiber-based composite nanofiber tissue scaffold produced with electrospinning method

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Measurements were taken using a microplate reader and cytotoxic analysis was performed by comparing with the values of the control group.. According to the data shown in Table 3, it was observed that the viscosity of the solution was provided by SF and PCL polymers in the combination of SF/PCL/PEO (S1), and the conductivity of the solution increased with the SF polymer..

Assessment of fermented broken rice extracts for their potential as functional ingredients in cosmeceutical products

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A microplate reader (VersaMax) was used to determine the quantity of dopachrome produced in the reaction mix- ture. The mixture was then in- cubated in the dark at room temperature. mL DQ™ elastin working solution was added into the mixture and al- lowed to react for 30 min in the dark at room temperature. Total phenolic and total flavonoid content of fermented BR extracts The total phenolic content of fermented broken rice extracts is shown in Fig.

Simple and convenient preparation of novel 6,8-disubstituted quinoline derivatives and their promising anticancer activities

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The amount of cell proliferation was assessed by determining the A450 nm of the culture media after the addition of the substrate solution using a microplate reader (Ryto, China). The data are presented as the means ± SEM of the 6 measurements for each cell type. In The Chemistry of Heterocyclic Compounds, Weissberger, A.

Mesenchymal stromal cells attenuate sevoflurane-induced apoptosis in human neuroglioma H4 cells

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In addition, the contents of fluorescent signals in the H4 cells were de- termined using a fluorometric microplate reader (Syn- ergy H1, BioTek, USA) at 488 nm.. The different groups of H4 cells were lyzed in cell lysis buffer. The relative levels of target protein to β-actin were determined by densitometric analysis using the Image J software..

Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata)

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After the sample turned yellow, the absorbance of A400nm was measured by a microplate reader, and the relative caspase 3 activity was calculated according to the ratio of the absorbance of the apoptosis- induced cells to the absorbance of the blank control cells (Caspase 3 Activity Assay Kit, G015–1-3, Nanjing Jian- cheng Bioengineering Institute, Nanjing, China). 2 Oceanic Consultation Center, Ministry of Natural Resources of the People ’ s Republic of China, Beijing 100071, China.

Effects of B2O3 (boron trioxide) on colon cancer cells: our first-step experience and in vitro results

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After finishing the experiment, the plates were instantly scanned in an ELISA Microplate Reader (BioTek, USA) at 440-nm wavelength and the percentage of cell viability for each group was assessed with the definition of the control cell viability as 100%.. Double staining (DS) of Hoechst dye with propidium iodide (PI) is one of the methods that provide the.

Design, synthesis, and antitumor evaluation of novel methylene moiety-tethered tetrahydroquinoline derivatives

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The optical density of the dissolved material was measured at 570 nm (reference wavelength: 750 nm) using an automated microplate reader (Bio-Rad iMark). Sytox AADvanced Dead Cell Stain Solution (1 µL) was added to each sample 5 min before the end of the incubation period. Measurements were taken with FL1 and FL3 lines of the flow cytometer (Accuri BD C6). Pseudomonas aeruginosa strain PAO1 was used for the evaluation of the swarming motility assay.

3-Hydroxy-7,8,9,10-tetrahydro-6H-benzo[c]chromen-6-one and 3-hydroxy-6H-benzo[c] chromen-6-one act as on-off selective fluorescent sensors for Iron (III) under in vitro and ex vivo conditions

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The plate was further incubated for 4 h in the incubator, and the resulting absorbance at 450 nm was measured by using a tunable microplate reader (VERSA max, Molecular Devices LLC, Sunnyvale, CA, USA).

Stimulation of wound healing process through ROS/RNS signals indirectly generated by N2/Ar micro-plasma - in vitro and in vivo studies

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The absorbance at 492 nm for the solution in 96-well plates as measured with a standard microplate reader (Multiskan EX Labsystems, Finland). The quantity of formazan product in association with the intensity of absorbance was directly proportional to the number of cultured living cells. The migration ability of the plasma- exposed cells was assessed. The test cells were first exposed to N 2 /Ar micro-plasma for 5, 10, or 15 sec.

Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

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After the water evaporated, isopropanol was added to extract Oil Red O for 10 min, and then, the absorbance of the solution was measured at 510 nm with a Multimode Microplate Reader (Thermo Fisher Scientific Inc., Suzhou, China)..

PHÁT HIỆN QUYẾT ĐỊNH KHÁNG NGUYÊN TRONG PHÂN TỬ PROTEIN AS16 CỦA ASCARIS SUUM, ỨNG DỤNG TRONG SẢN XUẤT VẮC-XIN TÁI TỔ HỢP PHÒNG BỆNH GIUN ĐŨA Ở HEO

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Phản ứng kết hợp giữa kháng nguyên và kháng thể trong ELISA được xác định bởi giá trị độ mật quang ở bước sóng 450 nm bằng máy đọc VERSA max microplate reader (Molecular devices, California, USA)..

Sinh Lý Bệnh Học ĐH Tân Tạo Khoa Y || Trường ĐH Y Khoa Vinh VMU

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Doc KQ bang may (ELISA microplate reader).Hinh anh minh hga nguyén ly ky thuat ELISAs 3. Ky thuat kép dé phat hign KN. K¥ thu@t ELISA canh tranh: Ky thuat canh tranh KN (gan men) dé phat hién KN. KY THUAT KHUECH TAN MIEN DICH (Ouchterlony double immunodiffusion) 1.Muc tiéu. Thue hanh ky thuat khuéch tan mién dich. Néu vét tia mo nhat, long KN+ KT khéng cao hode ky thuat chua phu hop.

Đa Dạng Chức Năng Của Quần Xã Vi Khuẩn Trên San Hô Ven Đảo Cát Bà và Long Châu, Góp Phần Thích Ứng Với Sự Thay Đổi Của Môi Trường

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Sau mỗi 24 giờ nuôi, bản thí nghiệm Biolog-Ecoplate được đo mật độ quang tại bước sóng 590 nm (đỉnh của tetrazodium) bằng máy Microplate Reader – BIO RAD Model 680 (Insam và Goberna, 2004)..