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Radical scavenging activity


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Magnetite nanoparticles−based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe

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A novel easy and low-cost DMPD-based ROS scavenging activity method was developed with the aid of Fe 3 O 4 NPs having peroxidase-like activity. MNPs catalyzed the degradation of H 2 O 2 to ROS (mainly hydroxyl radicals) which in turn converted DMPD to a colored radical cation, and antioxidants (when present) quenched • OH, thereby causing a decrement in the DMPD.

Variation of nutrients and antioxidant activity in seed and exocarp layer of some Persian pistachio genotypes

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The highest DPPH radical scavenging activity extracted from exocarp layer was observed in G3 (90.55. The obtained data refer to DPPH radical scavenging activity between some genotypes in exocarp layer and seed of pis- tachio genotypes. The chemical nature and phenolic content presented in exocarp layer and seed extracts could be attributed to antioxidant activity.. Lopes et al.

Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells

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The radical scavenging activity of the nanoemulsions was carried out using the colorimetric DPPH assay, which is based on reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH . The percentage radical scavenging activities of the nanoemulsion samples were calculated by comparing nanoformulations.. SDS is one of the most used solvents for formazan crystals, others being DMSO and 2-propanol. In the second phase of the study, cell based hepatoprotective activity was performed.

Antioxidant, anticholinesterase, and antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm.

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DPPH free radical scavenging activity (inhibition. DPPH free radical scavenging activity of the extracts, α-tocopherol, and BHT. ABTS cation radical scavenging activity (inhibition. ABTS cation radical scavenging activity of the extracts, α-tocopherol, and BHT. 16 In the present work, all of the extracts were inactive against acetylcholinesterase. 20–12 mm) at 30 mg/mL concentration against E. Anticholinesterase activity of the extracts and galanthamine at 200 µ g/mL.

Initial evaluation of antioxidant and antibacterial activities of several medicinal plant extracts collected in Vietnam

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DPPH Radical Scavenging Assay. extracts were diluted into tested concentrations (0.02 and 0.1 mg/ml). Free Radical Scavenging Activity. The free radical scavenging activity of ethanolic extracts of investigated plants are shown in Figure 1. Among the extracts, the mixture of ginger and kumquat possessed the highest activity (32% and 85% at the concentration of 0.02 and 0.1 mg/ml, respectively).

A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

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The observed radical scavenging activity and total phenolic contents for WGO and BCSO are in good alignment. 29 WGO and BCSO displayed lower radical scavenging activity than did BHA and BHT, the most commonly used synthetic antioxidants, as positive controls. DPPH radical scavenging activities of wheat germ oil (WGO) and black cumin seed oil (BCSO).

Total flavonoid content and antioxidant activity of ethanol and ethyl acetate extracts from accessions of Amomum compactum fruits

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Two in-vitro assays were performed used to investigate the antioxi- dant activity in ethanol and ethyl acetate extracts of sample accessions.. The CUPRAC method was tested according to Apak et al. The assay of DPPH radical scavenging activity was performed ac- cording to (Brand-Williams et al., 1995) with modi fi cation. ExpDes package in R was used to determine ANOVA and followed by a Scott-Knott test (Ferreira et al., 2014). Total fl avonoid content.

Antioxidant and hypoglycemic activities of extract and fractions of Rambutan seeds

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The rambutan peel extract exhibited higher antioxidant activity than seed extracts [18], rambutan peel possessed high DPPH free radical scavenging activity (IC 50 of 8.87 μg/ml) [19], high polyphenols content of rambutan peel contributes towards high free radical scavenging activity [20].

Secondary metabolites and in vitro biological activities of fractions from stemona collinsae craib root extract

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Higher phenolic content in the ethyl acetate fraction might be expected to exhibit various bioactivities such as antioxidant activity.. Antioxidant activity. DPPH radical scavenging activity. collinsae fractions and ascorbic acid, IC 50 values was calculated.. DPPH radical scavenging activity of S. collinsae root fractions compared with ascorbic acid. Sample IC 50 (μg/mL). n-Hexane fraction 3.258 c. Butanol fraction 4.884 b. Ethyl acetate fraction 0.676 a.

Chemical composition and biological activities of essential oils of two new chemotypes of Glebionis Cass

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There are studies on DPPH radical scavenging activity of essential oil of Chrysanthemum coronarium (Glebionis coronaria) in the literature. coronaria) essential oil did not have significant DPPH radical scavenging activity [9]. found that hydro-distilled essential oil obtained from the C. However, there is no study on the DPPH radical scavenging activity of the essential oil of Glebionis segetum.

Evaluation of the antioxidant activity and dosage of polyphenols in aqueous, hydroethanolic and hexane extracts of the bark of Spathodea campanulata P. Beauv. (Bignoniaceae)

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In Vitro evaluation of the antioxidant activity of aqueous, hydroethanolic and hexane extracts of Spathodea campanulata Free Radical Scavenging Activity. Hydrogen atom or electron donating abilities of the compounds were measured from the bleaching of the purple-colored methanol solution of 2,2-diphenyl-1-picryl hydrazyl (DPPH). This spectrophotometric assay uses the stable free radical, DPPH as a reagent (Parejo et al., 2000)..

Antioxidant activity and hepatoprotective effect of pomegranate peel and whey powders in rats

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From the data presented in Table 2, it could be noticed that the pomegranate peel powder (PPP) is a good source of total phen- olics and had a great free radical scavenging activity. While, whey powder (WP) recorded the least scavenging activity com-. These results are in agreement with those of Ibrahium (2010) and El-Kady et al. From the same table, it could be noticed that the relative weight of liver was signif- icantly higher in the IC group than in the other groups (Table 3).

Synthesis of E-stilbene azomethines as potent antimicrobial and antioxidant agents

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Antioxidant activity via inhibition of linoleic acid oxidation. Antioxidants are also effective in scavenging ROS related to the lipid peroxides providing protective health benefits.. The results of the inhibitory effects of the synthesized compounds (5a–5h) on linoleic acid peroxidase are summarized in Figure 8. Compounds 5a–5h exhibited linoleic acid peroxidase inhibition in the range of 46. The DPPH free radical scavenging activity of compounds 5a–5h..

Terpenoids, essential oil composition, fatty acid profile, and biological activities of Anatolian Salvia fruticosa Mill.

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DPPH free radical scavenging activity. The free radical scavenging activity of the samples was determined by the DPPH assay. Determination of the antioxidant activity with the β -carotene bleaching method. Measurement of superoxide anion radical scavenging activity of the samples was based on the standard method with slight modification. The ferric reducing power of the samples was determined according to the iron(III) reductive assay.

Synthesis and antioxidant activities of new nickel(II) complexes derived from 4-benzyloxysalicylidene-S-methyl/propyl thiosemicarbazones

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ABTS radical scavenging activities of thiosemicarbazones were significantly higher compared to nickel(II) complexes.. Since ABTS radical scavenging activity also uses for total antioxidant capacity determination in the literature, it confirms the parallelism between CUPRAC results and ABTS radical scavenging activities obtained in the study. The compounds were investigated for the effect of alkyl chain and the position of the methoxy group of the second aldehyde on antioxidant activity.

Study on color and antioxidant properties of rambutan seed fat as cocoa butter alternative

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The radical scavenging activity of the fat samples was evaluated according to the method of [26]. The analysis was performed based on the activity of the 2, 2-diphenyl-1- picrylhydrazyl (DPPH). The analysis was implemented based on inhibition of the DPPH radical and the absorbance was read at 517nm using the UV-160A spectrophotometer (Shimadzu Corp., Nagakyo-ku, Kyoto, Japan). The scavenging effect was conducted based on the percentage of DPPH radical scavenging activity.

Antioxidant properties of water-soluble phthalocyanines containing quinoline 5-sulfonic acid groups

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In this work, we used the DPPH free radical scavenging method to evaluate the antioxidant potential of the synthesized Pc compounds. The descending order of radical scavenging activity of the tested Pc compounds was 6 >. The Pc compounds showed lower DPPH activity in comparison to the known Trolox and BHT standards (Figure 1). The DPPH scavenging activities of metal-free Pc compounds were also measured.

Synthesis and biological evaluation of a new series of 4-alkoxy-2-arylquinoline derivatives as potential antituberculosis agents

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DPPH • radical scavenging activity was evaluated by using ascorbic acid as antioxidant standard, and the values are expressed as SC 50 (mg/mL), the concentration of the sample resulting in 50% scavenging of the radicals. Low SC 50 values represent high antioxidant activity..

Formation and characterization of mechanochemically generated free lignin radicals from olive seeds

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It is worth to note that even the noncryomilled olive seed lignin has some radical scavenging activity from the beginning (Figure 10b zero min), which confirms the antioxidant nature of the lignin (in good agreement with literature [18. The scavenging activity of the noncryomilled sample has enhanced over waiting time in DPPH solution, due to the migration of the present radicals from the bulk to the surface.