Tìm thấy 11+ kết quả cho từ khóa "Radical scavenging assay"
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In the DPPH-radical scavenging assay, compound 2 exhibited promising activity (IC µ M), as shown in Figure 3, while other compounds were inactive. Moreover, in the urease inhibition assay, only compound 1 showed moderate activity (43 ± 1% inhibition at 1 mM). Butylated hydroxy anisole (IC 50 = 0.22 mM) and thiourea (IC µM) were used as standard inhibitors in the radical-scavenging assay and the urease inhibition assay, respectively.. Alpha-glucosidase inhibition (IC 50) of com- pound 1.
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Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay. Solvent extraction of antioxidants from steam exploded sugarcane bagasse and enzymatic convertibility of the solid fraction. The antioxidant and free- radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides.
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In the assay, 0.5 g of BCSO and WGO was dissolved in 10 mL of hexane, vortexed for 1 min, and filtered. DPPH radical scavenging assay.
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The acetone, methanol, and water extracts indicated over 80% inhibition in ABTS cation radical scavenging assay at 100 µg/mL (Figure 3). None of the extracts had a cupric reducing effect.. Total phenolic and flavonoid contents of the extracts. of lipid peroxidation of the extracts, α-tocopherol, and BHT by β -carotene bleaching method.. DPPH free radical scavenging activity (inhibition. DPPH free radical scavenging activity of the extracts, α-tocopherol, and BHT.
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DPPH Radical Scavenging Assay. extracts were diluted into tested concentrations (0.02 and 0.1 mg/ml). Free Radical Scavenging Activity. The free radical scavenging activity of ethanolic extracts of investigated plants are shown in Figure 1. Among the extracts, the mixture of ginger and kumquat possessed the highest activity (32% and 85% at the concentration of 0.02 and 0.1 mg/ml, respectively).
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Therefore, we selected 30 by-products casually to investigate their antioxidant activity by using different antioxidant tests, including DPPH free radical scavenging assay, Ferric reducing/antioxidant power (FRAP) assay and determination of total phenolics contents.
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The 2,2 0 -azino-bis (3-ethyl- benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay was done according to the method of Re et al. Aliquot (100 ll) of each sample was mixed with 3 ml of the prepared ABTS working solution and the change in absorbance was observed at 734 nm. The ABTS radical scavenging capacity of the sample was calculated by the following formula:. The amount of TFC in the extracts was measured spectropho- tometrically according to Djeridane et al.
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The in vitro antioxidant activity of ethanolic extracts was assessed with two methods: the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and the ferrous ion chelating power.. The DPPH radical-scavenging capacity was measured by the method reported in Bouaziz et al.
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DPPH• radical scavenging assay. solution was prepared by mixing 7 mM ABTS and 2.45 mM potassium persulfate and left in a dark environment for about 16–20 h at room temperature until the day of the analysis. radical solution was mixed with 50 µL of the sample, vortexed, and incubated at room temperature for 20 min. A 50 μL of the sample was diluted by using 2.5 mL of distilled water. The carrier gas was helium with a flow rate of 1.0 mL/min. Phenolic compounds found in various parts of the A.
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The results of the DPPH radical scavenging assay suggest that compo- nents involving the extracts are capable of scavenging free rad- icals via electron- or hydrogen-donating mechanisms and thus might be able to prevent the initiation of deleterious free rad- ical mediated chain reactions in susceptible matrices.
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Extract and standard exhibited an IC50 value of 38.5 µg/ml and 15.5 µg/ml in Hydrogen peroxide Radical scavenging assay which are represented in the Figure 23.The hydroxyl radical and hydrogen peroxide together with superoxide anion arbitrate mechanism of the inflammation with cyclic nucleotides and eicosanoids as products. standard and graphically represented in Figure 28.The scavenging outcome sup surged with the cumulative concentrations of test compounds. rotundus had been evaluated for antioxidant
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Spearman’s rank correlation (tested on 8 antioxidants) was found ‘strong’ between the developed method and the ABTS (radical scavenging) assay..
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The antioxidant capacity of MeGa was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging assay (Trolox as the reference standard) with slight modifications (Yang et al., 2011). All of the experiments were conducted in triplicate following the protocol previously reported by Abdul Hisam et al. Metabolite profiling of the MeGa. Acute toxicity effect of the MeGa. Subacute toxicity effect of the MeGa.
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Antioxidant activity of P. tectorius extracts was estimated by DPPH radical scavenging assay and expressed as IC 50 . The ethyl acetate . The scavenging capacity of this fraction was around 77.1% at the concentration of 2 mg/ml, nearly equal to the scavenging capacity of ascorbic acid at 0,1 mg/ml. The DPPH scavenging capacity of the ethyl acetate fraction of P.
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DPPH radical scavenging activity. collinsae fractions and ascorbic acid, IC 50 values was calculated.. DPPH radical scavenging activity of S. collinsae root fractions compared with ascorbic acid. Sample IC 50 (μg/mL). n-Hexane fraction 3.258 c. Butanol fraction 4.884 b. Ethyl acetate fraction 0.676 a.
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In this work, we used the DPPH free radical scavenging method to evaluate the antioxidant potential of the synthesized Pc compounds. The descending order of radical scavenging activity of the tested Pc compounds was 6 >. The Pc compounds showed lower DPPH activity in comparison to the known Trolox and BHT standards (Figure 1). The DPPH scavenging activities of metal-free Pc compounds were also measured.
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The free radical scavenging capacities of the compounds were also tested in vitro determining the interaction of the stable free radical 2,2,diphenyl- 1-picrylhydrazyl (DPPH), and compounds 4a and 4b exhibited good antioxidant activities.. Impair- ment of the antioxidants and antioxidant systems could be related to increased oxidative stress.
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The rambutan peel extract exhibited higher antioxidant activity than seed extracts [18], rambutan peel possessed high DPPH free radical scavenging activity (IC 50 of 8.87 μg/ml) [19], high polyphenols content of rambutan peel contributes towards high free radical scavenging activity [20].
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The antioxidant activities of the synthesized samples were also determined by FRAP assay (Figure 3). 33,34 The method is based on measurement of the iron reducing capacities of the compounds. In general, FRAP results were similar to those of DPPH • radical scavenging activities.
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Antioxidant activity assessment was carried out using two methods: scavenging ability of naphthoxazoles and fused heterobenzoxazoles towards both DPPH� and FRAP.. DPPH scavenging ability of the samples was measured according to a recently reported procedure. 33 The results for the free radical scavenging activities of the samples are expressed as IC 50 values (where possible) and inhibition percentages of DPPH radical.