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Handbook of Microbiological Media, Fourth Edition part 40

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Preparation of Selective Supplement Solution: Add components to distilled/deionized water and bring volume to 20.0mL. Preparation of Medium: Add components, except differential lec- ithin solution and selective supplement solution, to distilled/deionized water and bring volume to 940.0mL. 18.0g Agar. 12.0g LiCl. 10.0g Yeast extract. 10.0g Enzymatic digest of casein. L -α-phosphotidylinositol ...40.0mL Selective supplement solution ...20.0mL. Composition per 20.0mL:. 10.0mg...

Handbook of Microbiological Media, Fourth Edition part 41

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0.5mg Wolfe’s mineral solution ...10.0mL Wolfe’s vitamin solution ...10.0mL NaHCO 3. 0.01g Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to approximately 500.0mL of water and adjust to pH 6.5 with KOH to dissolve the compound. 0.1g Cellosbiose solution...100.0mL Na 2 S·9H 2 O solution...10.0mL Trace element solution SL-10...1.0mL Selenite/tungstate solution ...1.0mL. Composition per 100.0mL:. deionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 42

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1.0mg Fructose solution...50.0mL NaHCO 3 solution ...30.0mL Trace elements solution SL-4 ...10.0mL. 0.2g Trace elements solution SL-6 ...100.0mL Trace Elements Solutions SL-6:. Composition per 50.0mL:. 5.0g Preparation of Fructose Solution : Add fructose to distilled/de- ionized water and bring volume to 50.0mL. Composition per 30.0mL:. 10.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring...

Handbook of Microbiological Media, Fourth Edition part 43

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Preparation of Medium: Add components, except NaHCO 3 solu- tion and rhamnose solution, to distilled/deionized water and bring vol- ume to 960.0mL. Aseptically and anaerobically add 20.0mL of sterile NaHCO 3 solution and 20.0mL of sterile rhamnose solution. 1.0mg Trace elements solution ...10.0mL Vitamin solution...10.0mL. L -Cysteine·HCl·H 2 O solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL. 0.01g Preparation...

Handbook of Microbiological Media, Fourth Edition part 44

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Aseptically add 50.0mL of sterile reductant solution. 10.0g Yeast extract. Clostridium thermolacticum Medium Composition per 1020.0mL:. L -Cysteine·HCl·H 2 O solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL Wolfe’s vitamin solution ...10.0mL Trace elements solution SL-6 ...3.0mL. 10.0mg p-Aminobenzoic acid. Composition per 10.0mL:. 0.3g Preparation of L -Cysteine·HCl·H 2 O Solution: Add L - cysteine·HCl·H 2 O to distilled/deionized...

Handbook of Microbiological Media, Fourth Edition part 45

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Preparation of Oxolinic Acid Solution: Add oxolinic acid to dis- tilled/deionized water and bring volume to 10.0mL. Preparation of Medium: To 930.0mL of sterile, cooled Columbia agar base, add sterile colistin sulfate, sterile oxolinic acid, and sterile, de- fibrinated horse blood. 20.0g K 2 HPO 4. 1.0mg Trimethylamine solution ...10.0mL. Composition per 10.0mL:. 1.0g Preparation of Trimethylamine Solution : Add...

Handbook of Microbiological Media, Fourth Edition part 46

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100.0g. 10.0g. Composition per 890.0mL:. 17.0g Agar. 15.0g NaCl. 2.5g Preparation of Soybean-Casein Digest Agar: Add components to distilled/deionized water and bring volume to 890.0mL. Composition per 100.0mL:. Preparation of Hemoglobin Solution: Add hemoglobin to dis- tilled/deionized water and bring volume to 100.0mL. 15.0g Pancreatic digest of gelatin. 12.0g Brain heart, solids from infusion. 1.0g Congo Red solution ...20.0mL. Composition...

Handbook of Microbiological Media, Fourth Edition part 47

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Bovine serum ...100.0mL Nystatin solution ...1.15mL. L- Cystine (1% solution) ...1.0mL Egg yolk emulsion ...10 eggs. 1.8g K 2 TeO 3 (2% solution) ...75.0mL. L- Cystine (1% solution) ...10.0mL Caution: Potassium tellurite is toxic.. Preparation of Solution A: Add components to distilled/deionized water and bring volume to 985.0mL. Composition per 10.0mL:. 10,000U Preparation of Nystatin Solution: Add nystatin to distilled/de-...

Handbook of Microbiological Media, Fourth Edition part 48

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1.0mg 2,3,5-Triphenyltetrazolium chloride solution ...10.0mL. Composition per 10.0mL:. 0.1g Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu- tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except 2,3,5-triphe- nyltetrazolium chloride solution, to distilled/deionized water and bring volume to 990.0mL. Aseptically add 10.0mL 2,3,5-triphenyltetrazolium chloride solution.. 20.0g K 2 SO 4. 20.0g Agar. 1.0mg Biotin ...1.0μg...

Handbook of Microbiological Media, Fourth Edition part 49

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17.0mg Carbohydrate solution...50.0mL. Composition per 100.0mL:. 10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL. Aseptically add 50.0mL of sterile carbohydrate solution. 15.0g KH 2 PO 4. 50.0g ZnSO 4 ·7H 2 O. 22.0g CaCl 2. Cytophaga agarovorans Agar (LMG Medium 99) Composition per 1001.0mL:. 30.0g Agar. 1.25mg Glucose solution ...10.0mL NaHCO 3 solution...

Handbook of Microbiological Media, Fourth Edition part 50

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Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Preparation of Medium: Add components, except glucose solu- tion, to distilled/deionized water and bring volume to 980.0mL. Aseptically add 20.0mL of sterile glucose solution. Pour into sterile Petri dishes in 20.0mL volumes.. 10.0g Proteose peptone. 10.0g Lactose. 10.0g Plant peptone No. 20.0g Cornmeal. 15.0g Supplement...

Handbook of Microbiological Media, Fourth Edition part 51

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10.0g Proteose peptone No. 10.0g Sodium deoxycholate. 10.0g Heart infusion solids. 10.0g Lactose. 10.0g Synthetic detergent No. 10.0g Sodium citrate. 10.0g NaCl. 15.0g. 10.0g Sucrose. 10.0g Glucose. 10.0g Antibiotic inhibitor ...10.0mL. Composition per 10.0mL:. 0.05g Acetone ...10.0mL Preparation of Antibiotic Inhibitor: Add cycloheximide and chloramphenicol to 10.0mL of acetone. Preparation of Medium: Add components to distilled/deionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 52

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Composition per 10.0mL:. 2.0g Preparation of Yeast Extract Solution: Add yeast extract to dis- tilled/deionized water and bring volume to 10.0mL. NaHCO 3 Solution : Composition per 10.0mL:. 3.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 10.0mL. deionized water and bring volume to 1.0L. 0.3mg Preparation of Trace Elements Solution: Add...

Handbook of Microbiological Media, Fourth Edition part 53

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Composition per 10.0mL:. 0.4g Preparation of Solution F: Add Na 2 S·9H 2 O to distilled/deionized water and bring volume to 10.0mL. 0.5g Preparation of Solution G: Add Na 2 S 2 O 4 to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: To 900.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order:...

Handbook of Microbiological Media, Fourth Edition part 54

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0.5mg Wolfe’s vitamin solution ...10.0mL NaHCO 3 solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL. L -Cysteine·HCl solution...10.0mL Wolfe's Mineral Elixir...1.0mL n-Decane ...1.0mL. Composition per 10.0mL:. 0.1g Preparation of L -Cysteine Solution : Add L -cysteine·HCl to dis- tilled/deionized water and bring volume to 10.0mL. Na 2 S·9H 2 O Solution : Composition per 10.0mL:. 0.1g Preparation of Na...

Handbook of Microbiological Media, Fourth Edition part 55

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AlCl 3 ·6H 2 O, 4.9% (w/v) ...5.0mL Na 2 CO w/v) ...1.6mL Preparation of Solution G (Artificial Sediment): Combine components. Composition per 20.0mL:. Rumen fluid, clarified ...20.0mL Preparation of Solution H: Sparge with 100% N 2 . Composition per 10.0mL:. 0.4g Preparation of Solution I: Add Na 2 S·9H 2 O to distilled/deionized water and bring volume to 10.0mL....

Handbook of Microbiological Media, Fourth Edition part 56

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0.1g Vitamin solution...10.0mL Trace elements solution SL-10 ...1.0mL. 10.0mg Thiamine-HCl·2H 2 O. 100.0mg ZnCl 2. 2.0mg HCl (25% solution)...10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Desulfotomaculum geothermicum Medium Composition per 1001.0mL:. Solution A...870.0mL Solution C ...100.0mL Solution D...10.0mL Solution E (Vitamin solution)...10.0mL Solution F...10.0mL Solution B (Trace elements solution...

Handbook of Microbiological Media, Fourth Edition part 57

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Desulfovibrio gabonensis Medium Composition per 1002.0mL:. 0.5mg Sodium lactate solution...10.0mL NaHCO 3 solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL Trace elements solution SL-10 with EDTA ...1.0mL Seven vitamin solution...1.0mL. Composition per 10.0mL:. 2.5g Preparation of Sodium Lactate Solution: Add sodium lactate to distilled/deionized water and bring volume to 10.0mL. 2.5g Preparation of NaHCO 3 Solution: Add NaHCO 3...

Handbook of Microbiological Media, Fourth Edition part 58

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Composition per 100.0mL:. 5.0g Preparation of Solution C : Add NaHCO 3 to distilled/deionized water and bring volume to 100.0mL. Filter sterilize under 100% CO 2 into a sterile, gas-tight 100.0mL screw-capped bottle.. Composition per 10.0mL:. 0.5g Preparation of Solution D: Add Na-benzoate to distilled/deionized water and bring volume to 10.0mL. 10.0mg Thiamine-HCl·2H 2 O. 0.4g Preparation of Solution G:...

Handbook of Microbiological Media, Fourth Edition part 59

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Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 100.0mL. Composition per 10.0mL:. 0.6g Preparation of Na-pyruvate Solution: Add Na-pyruvate to dis- tilled/deionized water and bring volume to 10.0mL. 2.0mg HCl (25% solution)...10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Add components, except...