Có 30+ tài liệu thuộc chủ đề "High-throughput sequencing"
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CircMarker: a fast and accurate algorithm for circular RNA detection. Non-canonical splicing joins 3’ and 5’ and forms the so-called circular RNA.. It is now believed that circular RNA plays important biological roles such as affecting susceptibility of some diseases.. During the past several years, multiple experimental methods have been developed to enrich circular RNA while degrade linear RNA. Although...
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Of the 12 randomly selected circRNAs with different expression levels, 10 showed consistent expression patterns based on high-throughput sequencing and quantitative real-time PCR analyses. Full list of author information is available at the end of the article. 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0. Utilization of heterosis has...
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improvement of high-temperature. Background: High-temperature stress inhibited the growth of cucumber seedlings. 2 ’ grown at high-temperature (42 °C/32 ° C) in an artificial climate box improved the high-temperature tolerance. Although there have been many reports on the response of microRNAs (miRNAs) to high-temperature stress, the mechanism by which exogenous Spd may mitigate the damage of high-temperature stress through miRNA-mediated...
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Watermelon (Citrullus lanatus L.) is one of the important agricultural crops worldwide. Results: Here we carried out computational and experimental analysis of miRNAs and phased small interfering RNAs (phasiRNAs) in watermelon by analyzing 14 small RNA profiles from roots, leaves, androecium, petals, and fruits, and one published small RNA profile of mixed tissues. One hundred and one 21 nucleotide (nt)...
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GRIM-Filter: Fast seed location filtering in DNA read mapping using. Conclusion: GRIM-Filter exploits 3D-stacked memory, which enables the efficient use of processing-in-memory, to overcome the memory bandwidth bottleneck in seed location filtering. We show that GRIM-Filter significantly improves the performance of a state-of-the-art read mapper. GRIM-Filter is a universal seed location filter that can be applied to any read mapper....
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For filtering, a classifier was trained and tested on each of the following: sequences of taxa in the database, sequences of taxa related to those in the database, and random sequences. Full list of author information is available at the end of the article. as “the neglected world of eukaryotes in metage- nomics” [3]. For example, in the NCBI database...
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Sugarcane (Saccharum spp.) originates from the tropics but is cultivated primarily in the sub-tropics where it frequently encounters cold stress. Nicotiana benthamiana plants transiently overexpressing miR156 exhibited better growth status, lower ROS levels, higher anthocyanin contents as well as the induction of some cold-responsive genes, suggesting its positive role in the plant cold stress response.. Conclusions: This study provides a...
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Identification and expression profiling of microRNAs involved in the stigma exsertion under high-temperature stress in tomato. Under heat stress conditions, 34 known and 35 novel miRNAs were differentially expressed in stamens, and 20 known and 10 novel miRNAs were differentially expressed in pistils. GO and KEGG pathway analysis showed that the predicted target genes of differentially expressed miRNAs were significantly...
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Methods: Here we performed a genome-wide discovery of phasiRNAs and its host PHAS loci in P. We also used RLM 5’-RACE to validate some of the identified phasiRNA targets.. notoginseng, 204 and 90 PHAS loci that encoded 21 and 24 nucleotide (nt) phasiRNAs, respectively, were identified. We also found that miR171 with 21 nt triggers the generations of 21 nt...
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DOI: https://doi.org http://www.vjs.ac.vn/index.php/jmst. Metagenomics analysis of marine eukaryotic community in water and sediments at Lang Co - Da Nang sea by throughput 18S rRNA gene sequencing. The present study applied metagenomics to characterize the diversity and relative occurrence of eukaryotic organisms in the sea water (LC05.W and LCDN.W) and sediment (LC05.S and LCDN.S) samples collected at the Lang Co -...