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Handbook of Microbiological Media, Fourth Edition part 100

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10.0g CaCl 2 ·2H 2 O. 9.25mg Metals mL Preparation of Modified Hutner’s Basal Salts: Add nitrilotria- cetic acid to 500.0mL of distilled/deionized water. Composition per 100.0mL:. 0.018g Preparation of Metals “44”: Add components to distilled/deion- ized water and bring volume to 100.0mL. Preparation of Medium: Add components, except modified Hut- ner’s basal salts, to distilled/deionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 101

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Use: For the cultivation of Yersinia enterocolitica from foods.. Composition per liter:. Use: For the cultivation and maintenance of Aquaspirillum magnetot- acticum.. Magnetospirillum Medium (DSMZ Medium 380) Composition per liter:. Use: For the cultivation of Magnetospirillum magnetotacti- cum=Aquaspirillum magnetotacticum, and Magnetospirillum gryph- iswaldense.. Magnetospirillum 2 Medium Composition per liter:. Use: For the cultivation and maintenance of Magnetospirillum gryph- iswaldense.. Use:...

Handbook of Microbiological Media, Fourth Edition part 102

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15.0g MnSO 4 ·H 2 O. 10.0g MnCO 3. 15.0g Peptone. Solution A...100.0mL Solution B ...10.0mL. Composition per 100.0mL:. 33.4g Preparation of Solution A: Add FeSO 4 ·7H 2 O to distilled/deion- ized water and bring volume to 100.0mL. Composition per 10.0mL:. 0.2g Preparation of Solution B: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Preparation of...

Handbook of Microbiological Media, Fourth Edition part 103

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15.0g Pancreatic digest of casein. 0.2g Seawater...700.0mL. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 0.2g Seawater...500.0mL. Preparation of Medium: Add components to seawater and bring volume to 1.0L. Solution A ...980.0mL Solution B ...10.0mL Solution C ...10.0mL. Composition per 980.0mL:. 25.0g. 1.0mg Preparation of Solution A: Add components to distilled/deionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 104

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Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 30.0g Agar. 15.0g Beef extract. 10.0g Peptone. 10.0g. 12.0g Hemoglobin. 10.0g Pancreatic digest of casein. 1.0g Supplement solution ...10.0mL VCAT inhibitor ...10.0mL. Composition per liter:. 100.0g. Composition per 10.0mL:. 0.02g Preparation of VCAT Inhibitor: Add components to distilled/de- ionized water and bring volume to 10.0mL. Preparation of...

Handbook of Microbiological Media, Fourth Edition part 105

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cally add 80.0mL of sterile egg yolk emulsion, 50%. Pour into sterile Petri dishes in 20.0mL volumes.. 2.0g Egg yolk emulsion ...100.0mL MgSO 4 (5% solution) ...0.2mL. Preparation of Medium: Add components, except egg yolk emul- sion, to distilled/deionized water and bring volume to 900.0mL. Aseptically add 100.0mL of sterile egg yolk emulsion. Pour into ster- ile Petri dishes in...

Handbook of Microbiological Media, Fourth Edition part 106

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2.0mg HCl (25% solution)...7.7mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Preparation of Medium: Aseptically and anaerobically combine 1000.0mL solution A, 10.0mL solution B and 10.0mL solution C.. Aseptically and anaerobically add 5.0mL vitamin solution and 1.0mL trace elements solution SL-10. 0.05g Chloroacrylic acid solution...20.0mL Trace elements solution SL-4 ...10.0mL....

Handbook of Microbiological Media, Fourth Edition part 107

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0.1g Methylamine solution ...30.0mL Trace metal solution (Kelly solution T)...10.0mL. Composition per 10.0ml:. 0.5g Preparation of Methylamine Solution: Add components to dis- tilled/deionized water and bring volume to 10.0mL. 0.2g Preparation of Trace Metal Solution: Add EDTA to 400.0mL distilled/deionized water. Adjust pH to 6.0 using 1M NaOH (ap- proximately 24.0mL). Preparation of Medium: Add components, except trace metal so-...

Handbook of Microbiological Media, Fourth Edition part 108

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Composition per 100.0mL:. 439.8mg Preparation of Lilly and Barnett Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Preparation of Oligo Solution: Combine 1.0mL of Lilly and Bar- nett solution and 0.66mL of 1% Hoagland solution.. 10.0g Neopeptone. 10.0g Peptone. 10.0g. 20.0g Glucose. 0.25g Phosphate buffer solution ...50.0mL Phosphate Buffer Solution:. Composition per 1040.0mL:. 20.0g Agar. 15.0g Sucrose....

Handbook of Microbiological Media, Fourth Edition part 109

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Composition per 100.0mL:. 8.0g Preparation of Sodium Carbonate Solution: Add Na 2 CO 3 to distilled/deionized water and bring volume to 100.0mL. Composition per 20.0mL:. cysteine·HCl·H 2 O to 10.0mL of distilled/deionized water. In a separate tube, add Na 2 S·9H 2 O to 10.0mL of distilled/de- ionized water. 0.01g Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL...

Handbook of Microbiological Media, Fourth Edition part 110

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use inject, for each 5.0mL medium, 0.2mL sterile NaHCO 3 solution, 0.05mL sterile Na 2 S·9H 2 O solution, and 0.05mL sterile methanol so- lution. Methanobacterium II Medium (DSMZ Medium 825) Composition per 1065.0mL:. 0.5mg NaHCO 3 solution ...40.0mL Na-formate solution ...15.0mL Trace elements solution ...10.0mL Vitamin solution...10.0mL Na 2 S·9H 2 O solution ...10.0mL. Composition per 10.0mL:. 5.0g Preparation...

Handbook of Microbiological Media, Fourth Edition part 111

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Composition per 100.0mL:. 13.6g Preparation of Sodium Acetate Solution: Add 13.6g of sodium acetate·3H 2 O to distilled/deionized water and bring volume to 100.0mL. 0.013g Preparation of Pantoyllactone Solution: Add pantoyllactone to distilled/deionized water and bring volume to 100.0mL. 0.2g Preparation of Iron Stock Solution: Add Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O to 5.0mL of...

Handbook of Microbiological Media, Fourth Edition part 112

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Vitamin solution...10.0mL. L -Cysteine-HCl·H 2 O solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL. 0.3mg Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. 10.0mg Thiamine-HCl·2H 2 O. Composition per 10.0mL:. 0.3g Preparation of L -Cysteine-HCl·H 2 O Solution: Add L -cyste- ine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL.. Na 2...

Handbook of Microbiological Media, Fourth Edition part 113

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0.3mg Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Na 2 S·9H 2 O Solution:. Composition per 10.0mL:. Na 2 S·9H 2 O. 0.3g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. 2.0g Preparation of Trimethylamine·HCl Solution: Add trimethyl- amine·HCl...

Handbook of Microbiological Media, Fourth Edition part 114

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1.0mg Trace elements solution ...10.0mL Vitamin solution...10.0mL. L -Cysteine·HCl·H 2 O solution ...10.0mL Na 2 S·9H 2 O solution ...10.0mL Methanol ...5.0mL. 0.01g Preparation of Trace Elements Solution: Add nitrilotriacetic acid to approximately 500.0mL of distilled/deionized water. 10.0mg Calcium DL -pantothenate. Composition per 10.0mL:. 0.25g Preparation of L -Cysteine·HCl·H 2 O Solution: Add L -cyste- ine·HCl·H 2 O to distilled/deionized...

Handbook of Microbiological Media, Fourth Edition part 115

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0.01g Preparation of Trace Minerals Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. 10.0mg p-Aminobenzoic acid. deionized water and bring volume to 1.0L. 1.25g Preparation of L -Cysteine/Na 2 S Solution: Gently heat and bring 75.0mL of distilled/deionized water to boiling. Adjust pH to 10.0 with 3N NaOH. Bring volume to 100.0mL with distilled/. deionized water. Composition per 100.0mL:....

Handbook of Microbiological Media, Fourth Edition part 116

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Preparation of Carbonate Solution: Add Na 2 CO 3 to distilled/de- ionized water and bring volume to 10.0mL. Composition per 10.0mL:. 18.0g Preparation of Thiosulfate Solution: Add NaS 2 O 3 ·5H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except acetate, vitamin, carbonate, thiosulfate,...

Handbook of Microbiological Media, Fourth Edition part 117

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0.026g NaHCO 3 solution ...10.0mL. Composition per 10.0mL:. 0.06g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except NaHCO 3 solu- tion, to distilled/deionized water and bring volume to 990.0mL. Aseptical- ly add 10.0mL of sterile NaHCO 3 solution. (LMG Medium 258) Composition per liter:. 10.0g...

Handbook of Microbiological Media, Fourth Edition part 118

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1.0mg Biotin ...0.5mg Middlebrook ADC enrichment ...100.0mL Glycerol ...5.0mL. Composition per 100.0mL:. 0.003g Preparation of Middlebrook ADC Enrichment: Add compo- nents to distilled/deionized water and bring volume to 100.0mL. Preparation of Medium: Add glycerol to 900.0mL of distilled/de- ionized water and add remaining components, except Middlebrook ADC enrichment. Aseptically add 100.0mL of sterile Middlebrook ADC enrichment.. Middlebrook OADC enrichment ...100.0mL...

Handbook of Microbiological Media, Fourth Edition part 119

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10.0mg Preparation of Vitamin Solution: Add components to distilled/. Composition per 10.0mL:. 645.0mg Preparation of Cyanuric Acid Solution: Add cyanuric acid to 10.0mL distilled/deionized water. Aseptically add 10.0mL cyanuric acid solution, 10.0mL glyc- erol solution, and 10.0mL vitamin solution. 1.0g Hutner’s mineral base ...20.0mL 2,4-Dichlorobenzoate solution...10.0mL. 9.25mg Metals mL Preparation of Hutner’s Mineral Base: Add nitrilotriacetic acid to 500.0mL of...