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Handbook of Microbiological Media, Fourth Edition part 120

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Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 5.0mg Methanol ...5.0mL Trace minerals solution...1.0mL. 25.0mg Preparation of Trace Minerals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Preparation of Medium: Add components, except methanol, to distilled/deionized water and bring volume to 1.0L. 40.0mg CaCl 2. 15.0mg FeSO 4. 1.0mg Trace minerals solution...10.0mL...

Handbook of Microbiological Media, Fourth Edition part 121

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deionized water and bring volume to 10.0mL. Composition per 10.0mL:. 1.5g Preparation of Thiosulfate Solution: Add NaS 2 O 3 ·5H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. 0.3mg Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Preparation of Medium: Add components, except thiosulfate, bi- carbonate, and vitamin solutions, to distilled/deionized...

Handbook of Microbiological Media, Fourth Edition part 122

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Solution G ...10.0mL Solution B ...1.0mL Solution C ...1.0mL Solution D ...1.0mL. Composition per 940.0mL:. 0.5mg Preparation of Solution A: Prepare under 80% N 2 + 20% CO 2 gas atmosphere. Add components to distilled/deionized water and bring vol- ume to 940.0mL. 190.0mg MnCl 2 ·4H 2 O. 100.0mg ZnCl 2. 70.0mg Na 2 MoO 4 ·2H 2 O. 36.0mg...

Handbook of Microbiological Media, Fourth Edition part 123

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Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. 20.0g Plant peptone. 20.0g Plant extract. 15.0g Glycine anhydride. 10.0g. 0.5g Sheep blood, defibrinated ...50.0mL Selective supplement solution ...10.0mL. Composition per 10.0mL:. 0.2g Preparation of Selective Supplement Solution: Add cyclohex- imide to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except...

Handbook of Microbiological Media, Fourth Edition part 124

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1.0g Glycerin ...5.0mL. Distribute into tubes in 11.0mL volumes. 10.0g NaCl. Distribute into tubes in 4–5.0mL volumes.. 10.0g Agar. Distribute into tubes in 4.0mL volumes. 3.0g Triphenyltetrazolium chloride solution ...5.0mL. Composition per 5.0mL:. 0.1g Preparation of Triphenyltetrazolium Choride Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 5.0mL. Aseptically add 5.0mL triphenyltetrazolium chloride so- lution. Distribute into screw-...

Handbook of Microbiological Media, Fourth Edition part 125

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20.0g Peptone. 10.0g Plant extract. 20.0g Plant peptone no. 10.0g Sodium acetate. 20.0g Casein peptone, tryptic digest. 10.0g Meat extract. 10.0g Yeast extract. 0.05g Tween™ 80...1.0mL. 15.0g Pancreatic digest of casein. 10.0g Beef extract. 50.0mg Carbohydrate solution...100.0mL. Composition per 100.0mL:. 2.0g Preparation of Carbohydrate Solution: Add components to dis- tilled/deionized water and bring volume to 100.0mL. Preparation of Medium: Add...

Handbook of Microbiological Media, Fourth Edition part 126

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Composition per 100.0mL:. 0.5mg Preparation of Vitamin Mix: Add components to distilled/deion- ized water and bring volume to 100.0mL. 12.0g (NH 4 ) 2 SO 4. 2.0mg Vitamin mix ...1.0mL Preparation of MSV Agar: Add components to distilled/deionized water and bring volume to 1.0L. MSVP Agar Composition per 984.0mL:. 15.0g HEPES (N-[2-hydroxyethyl]piperazine-. 0.02g FeSO 4 (10mM solution) ...1.0mL Sodium pyruvate...

Handbook of Microbiological Media, Fourth Edition part 127

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2.0mg HCl (25% solution)...10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. deionized water and bring volume to 950.0mL. Asep- tically and anaerobically add 10.0mL of sterile Wolfe’s vitamin solu- tion, 10.0mL of sterile sodium malate solution, 10.0mL of sterile sodium pyruvate solution, 10.0mL of sterile NaHCO 3 solution, 10.0mL of...

Handbook of Microbiological Media, Fourth Edition part 128

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Composition per 700.0mL:. 1.0g Preparation of Basal Medium: Add components to distilled/de- ionized water and bring volume to 700.0mL. Composition per 100.0mL:. 25.0g Preparation of Fresh Yeast Extract Solution : Add the live Bak- er’s yeast to 100.0mL of distilled/deionized water. Mycoplasma Broth Composition per 950.0mL:. Composition per 680.0mL:. Preparation of PPLO Broth without Crystal Violet: Add components to distilled/deionized...

Handbook of Microbiological Media, Fourth Edition part 129

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Preparation of Medium: Add components, except solution A, so- lution B, and trace elements solution SL-4, to distilled/deionized water and bring volume to 900.0mL. 0.1g Sucrose solution...10.0mL Vitamin solution...10.0mL Cysteine solution...10.0mL Bicarbonate solution ...10.0mL Carbonate solution ...10.0mL Trace elements solution SL-10 ...1.0mL. 0.3mg Preparation of Trace Elements Solution SL-10: Add nitrilotri- acetic acid to 500.0mL of distilled/deionized water. Sucrose Solution...

Handbook of Microbiological Media, Fourth Edition part 130

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Preparation of Medium: Add components, except vitamin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Aseptically add 10.0mL of sterile vitamin solution. 20.0g Agar. 0.1g Sucrose-agar solution...200.0mL Biotin solution ...1.0mL Trace elements solution ...0.1mL. Composition per 200.0mL:. 20.0g Preparation of Sucrose-Agar Solution: Add components to dis- tilled/deionized water and bring volume to 200.0mL. Composition per 10.0mL:. 50.0μg Preparation...

Handbook of Microbiological Media, Fourth Edition part 131

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Composition per 100.0mL:. 2.0g Preparation of Solution A: Add CaCl 2 to distilled/deionized water and bring volume to 100.0mL. 20.0g Preparation of Solution B: Add MgSO 4 ·7H 2 O to distilled/deion- ized water and bring volume to 100.0mL. 0.1g Preparation of Solution C: Add chelated iron to distilled/deion- ized water and bring volume to 100.0mL. Composition per liter:. 41.4g...

Handbook of Microbiological Media, Fourth Edition part 132

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Nutrient Agar No. 15.0g NaCl. Nutrient Agar with Maltose Composition per liter:. 15.0g Maltose. Use: For the cultivation and maintenance of Nocardia corynebacteroi- des.. 2 Composition per liter:. 10.0g Peptone. 10.0g NaCl. Use: For the cultivation and maintenance of a wide variety of bacteria.. 20.0g Peptone. Use: For the cultivation and maintenance of Agromonas oligotrophica.. 2 Diluted 1/100 (DSMZ Medium...

Handbook of Microbiological Media, Fourth Edition part 133

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3.0g Streptomycin solution ...10.0mL. Composition per 10.0mL:. Preparation of Streptomycin Solution: Add streptomycin to dis- tilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL.. 2 Composition per liter:. 10.0g Peptone. 10.0g NaCl. 120.0g Pancreatic digest of gelatin. 500.0g Gelatin. 120.0g Tryptose. 120.0g Pancreatic digest of casein....

Handbook of Microbiological Media, Fourth Edition part 134

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20.0g Vitamin-free casamino acids. 10.0g Diammonium hydrogen citrate. 20.0μg Tween™ 80...2.0mL. 13.0g Malt extract. 0.5g Vitamin solution...10.0mL Vitamin Solution:. Composition per 10.0mL:. deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except vitamin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Aseptically add 10.0mL of sterile vitamin so- lution. 0.08g Carbohydrate solution...100.0mL. Composition per 100.0mL:....

Handbook of Microbiological Media, Fourth Edition part 135

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Aseptically add 10.0mL of sterile antibiotic inhibitor. 23.0g LiCl. 15.0g Agar. 10.0g NaCl. 0.5g Antibiotic inhibitor ...10.0mL. Composition per 10.0mL:. 2.0mg Ethanol (50% solution) ...10.0mL Preparation of Supplement Mix: Add components to 10.0mL of ethanol. Preparation of Medium: Add components, except supplement mix, to distilled/deionized water and bring volume to 990.0mL. Aseptically add 10.0mL of ster- ile supplement mix. 0.03g...

Handbook of Microbiological Media, Fourth Edition part 136

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Distribute while stir- ring into screw-capped tubes in 10.0mL volumes. Composition per 100.0mL:. 0.5g Ethanol, absolute...100.0mL Preparation of Solution B: Add TEM-4T and stigmasterol to 100.0mL of hot ethanol. Composition per 500.0mL:. 0.5g Preparation of Solution C: Add components to distilled/deionized water and bring volume to 500.0mL. Preparation of Medium: Combine 500.0mL of solution C, 10.0mL of solution A, and...

Handbook of Microbiological Media, Fourth Edition part 137

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Composition per 10.0mL:. 0.68g Preparation of 2,3-Butanediol Solution: Add 2,3-butanediol to distilled/deionized water and bring volume to 10.0mL. 2.0mg HCl (25% solution)...10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Add distilled/deionized water and bring volume to 1.0L. Add components, except NaHCO 3 solution, Na 2 S·9H 2 O solution, 2,3-butanediol...

Handbook of Microbiological Media, Fourth Edition part 138

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10.0g Peptone. 10.0g NaCl. 10.0g Beef extract. 10.0g Na 2 HPO 4. Distribute into bottles in 100.0mL volumes. 15.0g Soluble starch. 0.2g Na 2 CO 3 solution...100.0mL Na 2 CO 3 Solution:. Composition per 100.0mL:. 10.0g Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3 to distilled/de- ionized water and bring volume to 100.0mL. Preparation of Medium:...

Handbook of Microbiological Media, Fourth Edition part 139

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Distilled water...860.0mL Preparation of Solution B: Autoclave distilled water for 15 min at 15 psi pressure–121°C in a cotton-stoppered Erlenmeyer flask. Composition per 100.0mL:. 2.0mg Preparation of Solution C: Add vitamin B 12 to distilled/deionized water and bring volume to 100.0mL. 7.5g Preparation of Solution E : Add NaHCO 3 to distilled/deionized water and bring volume to 100.0mL. Filter sterilize...