Có 20+ tài liệu thuộc chủ đề "thí nghiệm thực nghiệm"
tailieu.vn Xem trực tuyến Tải xuống
Reference electrodes with pH meters, 77–78 in pH meters, 80 Refillable electrodes. Regulating eukaryotic expression, 509–510. in hybridization in Western blotting Reproducibility. of DNA purification methods, 169 in electrophoresis, 341–343 improving in electrophoresis, 353 with polymerase chain reactions, 298 of storage phosphor images, 443 Reproduction, of research results, 8 Research. Resins, in RNA purification, 210 Resolution. of labels, 405–406 of...
tailieu.vn Xem trực tuyến Tải xuống
MOLECULAR BIOLOGY PROBLEM SOLVER. Molecular Biology Problem Solver: A Laboratory Guide. Gerstein Copyright © 2001 by Wiley-Liss, Inc.. MOLECULAR BIOLOGY. PROBLEM SOLVER. A LABORATORY GUIDE. A JOHN WILEY &. SONS, INC., PUBLICATION. In all instances where John Wiley &. Sons, Inc., is aware of a claim, the product names appear in initial capital or all capital letters. Readers, however, should...
tailieu.vn Xem trực tuyến Tải xuống
Aoyagi, Kazuko, Millennium Pharmaceuticals, Inc., Cambridge, MA Bell, Peter A., Orchid BioSciences, Inc., Princeton,. NJ, [email protected]. Bonventre, Joseph A., New England Biolabs, Inc., Beverly, MA, [email protected]. Booz, Martha L., Bio-Rad Laboratories, Hercules, CA, [email protected]. Bruner, Brian, Ambion, Inc., Austin, TX. Dharmaraj, Subramanian, Ambion, Inc., Austin, TX Englert, David F., Packard Bioscience, Meriden, CT, [email protected]. Franciskovich, Phillip P., Motorola Life Sciences,...
tailieu.vn Xem trực tuyến Tải xuống
As discussed earlier, few research projects proceed exactly as planned, and these changes might require a change in the nature and scope of your reporting.. Ideally there will be only one tenable interpretation of the data, but this is a rare outcome. Providing a fair treatment of the various interpretations in the report should lead to a dis- passionate discussion...
tailieu.vn Xem trực tuyến Tải xuống
If the description isn’t complete, detailed, and accurate, make the company do it again. If some- thing goes wrong, it will be your only proof of what the supplier promised to do for you.. There is nothing inherently negative in the word “problem.” Its origin lay in phrases meaning “anything thrown forward” or “to lay before.” A problem provides opportunities...
tailieu.vn Xem trực tuyến Tải xuống
*Editor’s note: Yelling rings most effectively in the ears of upper management, not low-level personnel.. How Does a Buffer Control the pH of a Solution. What Is the Storage Lifetime of a Buffer. Editor’s note: Many, perhaps most, molecular biology procedures don’t require perfection in the handling of reagents and solution preparation. What Grades of Water Are Commonly Available in...
tailieu.vn Xem trực tuyến Tải xuống
It’s a lot like packing your own parachute—it’s your responsibility to prepare your important solutions. It is a good practice to use secondary spill containers (e.g., polypropylene or Teflon TM trays) in the flammables cabinet if they are not already built into the design.. How tempting to prepare a fresh solution in a volumetric flask and store it in the...
tailieu.vn Xem trực tuyến Tải xuống
Can You Perform Measurements in the. 108 What Is the Extinction Coefficient of an. 108 What Are the Strengths and Limitations of the Various. How Can the Characteristics of a Sample and the. Similarly moisture is generated if the sample is not allowed to reach the temperature of the weighing instrument. You the researcher are also a source of moisture...
tailieu.vn Xem trực tuyến Tải xuống
A broken or leaking sample container can seriously damage a centrifuge by knocking the rotor out of balance or exposing mechanical and electrical components to harsh chemicals.. If unsure, contact the tube manufacturer to assess compatability.. ask the rotor manufacturer about the availabil- ity of specialized equipment.. When in doubt about g force limitations, contact the tube’s manufac- turer. Confirm...
tailieu.vn Xem trực tuyến Tải xuống
All of the internal components of the pipette must be tested to determine that they are fully functional. The first thing that should be checked is the free movement of the piston. The piston should move up and down very smoothly. Next, verify that the internal parts are working properly by performing a leak test. Although this test does not...
tailieu.vn Xem trực tuyến Tải xuống
In order for the meter and electrode system to determine the pH of a sample, it must compare the sample to known values or standards. These buffers are used to generate a calibration curve plotting the known pH value versus the measured millivolts to determine the pH of a sample. The millivolt measurement of the sample is plotted on the...
tailieu.vn Xem trực tuyến Tải xuống
If the manufacturer’s instructions are followed and product ratings adhered to, a quality meter should last many years. Pro- tection of the meter from liquids, wiping up spills and respectful use gives long life. If the meter is to be used in harsh environments, use a meter rated rated for such work. Precipitates at the Electrode Junction. The precipitate that...
tailieu.vn Xem trực tuyến Tải xuống
If it is comprised of a solid block of glass or quartz and you see no seams within the cuvette, you can soak it in con- centrated nitric or sulfochromic acids (but not HF) for limited periods of time. The interior sample chambers of seamed cuvettes can be treated with acid but not the seams. What are the possible contaminants?...
tailieu.vn Xem trực tuyến Tải xuống
Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. 118 Are You Aware of the Potential Hazards during the. Setup, Execution, and Cleanup of the Planned. 122 What Are the Potential Sources of Contamination. 124 How Should You Maintain Microbial Strains in the. What Precautions Should Be Taken with Experimental Animals. What Precautions Should Be...
tailieu.vn Xem trực tuyến Tải xuống
Rough surfaces or bubbles in the agar can lead to exces- sive vibration of the loop. Use enough disinfectant to mediate the decontamination of the supernatant, and a container large enough to avoid overflow. Rinse the funnel with more disinfectant to handle any residual supernatant clinging to the funnel wall.. Even for solutions deemed to be safe, it is good...
tailieu.vn Xem trực tuyến Tải xuống
The answer to both of the above questions is a resounding yes!. Leave your lab wear, including lab coats, in the lab. The more familiar you become with the operational guidelines and functional capabilities of the facility, the more likely you will get the materials you need when you need them.. do the work will benefit you in the long...
tailieu.vn Xem trực tuyến Tải xuống
Your Count-Rate Meter Detects Radiation on the Outside of a Box Containing 1 mCi of a 32 P Labeled. Isn’t This a Dangerous. 153 How Do You Quantitate the Amount of Radioactivity. 155 Storing Radioactive Materials. 156 What Is the Stability of a Radiolabeled Protein or. 159 Monitoring Technology: What’s the Difference. The information within this chapter is designed as...
tailieu.vn Xem trực tuyến Tải xuống
It is always prudent to wear some thin plastic gloves when dealing with radioactive materials, espe- cially when they first arrive and you have no indication on whether or not they are contaminated.. The amount of contamina- tion considered significant will differ, depending on whether the activity is on the primary container, secondary container, or the outer package. Your Count-Rate...
tailieu.vn Xem trực tuyến Tải xuống
If you find yourself becoming stressed while handling radioac- tivity, or if that “incessant clicking sound” of the count-rate meter is causing a heightened sense of alarm, you can always step away from the bench to put things into perspective. How Can You Organize Your Work Area to Minimize Your Exposure to Radioactivity?. If feasible, select bench space at the...
tailieu.vn Xem trực tuyến Tải xuống
Too much sample can cause an increase in the viscosity of the DNA preparation and lead to shearing of genomic DNA. Free-radical oxidation seems to be a key player in breakdown and ethanol is the best means to control this process (Evans et al., 2000).. Storage at 4°C is only recommended for short periods (days) (Krajden et al., 1999). RNA...