Genome-wide identification, characterization, and expression analysis of tea plant autophagy-related genes (CsARGs) demonstrates that they play diverse roles during development and under abiotic stress
- In addition, the expression levels of 10 CsARGs were higher in the cold-resistant cultivar ‘ Longjing43 ’ than in the cold-susceptible cultivar. - To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.. - The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.. - Identification of CsARGs in tea plants. - Bioinformatic ana- lysis results showed that as a type of biological macro- molecule, the CsARG ORF lengths varied from 285 to 7410 bp, the corresponding numbers of deduced amino acids ranged from 94 to 2469 aa, and the molecular weights ranged from 10.51 to 276.87 kD. - These results indicated that these CsARGs play im- portant roles in the response of tea plants to cold. - In the present study, a total of 35 CsARGs were identified in the tea plant gen- ome. - specific subunit in the phosphatidylinositol 3-kinase (PI3-kinase) complex. - Under various abiotic stress conditions, most CsARGs were induced at different treatment time points, which indicated that CsARGs play central roles in the response to abiotic stress in tea plants. - In addition, 10 CsARGs were more highly expressed in the cold-resistant cultivar than in the cold-susceptible cultivar during CA periods;. - Three-year-old clonal potted seedlings of the ‘LongJ- ing43’ cultivar, which were planted in the greenhouse of the Tea Research Institute of the Chinese Academy of Agricultural Sciences (TRI, CAAS, N30°10′, E120°5. - Both tea cultivars were 18 years old and cultivated at the Tea Research Institute of the Chinese Academy of Agricultural Sciences (TRI, CAAS, N30°10′, E120°5. - First, ‘autophagy’ or ‘ATG’ as a keyword was searched in the Tea Plant Information Archive database (TPIA, http://tpia.teaplant.org/index.html) [88]. - Briefly, the ATG protein sequences of Arabidopsis were regarded as references to query the matched HMM from the Pfam database (http://pfam.xfam.org. - Bioinformatics analysis of CsARGs in tea plant. - expasy.org/protparam. - wolfpsort.org. - To obtain the promoter sequences, the genome sequences of each CsARG gene were down- loaded from the two published tea plant genomes (‘Shu- ChaZao’ and ‘YunKang10’) in the TPIA database. - The conserved domains of CsATG8s were identified using the MEME website (http://memesuite.org/) with optimum motifs. - The online version contains supplementary material available at https://doi.. - org/10.1186/s . - https://doi.org/10.4161/auto.2092.. - doi.org/10.4161/auto.7.7.14733.. - https://doi.org . - https://doi.org/10.1016/j.semcdb . - https://doi.org/1 0.1007/s . - https://doi.org/10.1 016/j.tplants . - https://doi.org/10.1016/s . - https://doi.org/10.1093/jxb/erx482.. - https://doi.org/10.1111/j.1365-313X.2005.. - https://doi.org/10.1074/jbc.M204630200.. - https://doi.org/10.1104/pp.011024.. - https://doi.org/10.1038/nrm2708.. - https://doi.org/10.1016/j.devcel . - https://doi.org/10.1038/ncb2788.. - 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