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Osmotic stress


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Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

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In the present study and 3050 significantly different expression genes were identified in salinity stress, alkalinity stress and saline-alkaline stress, respect- ively. Numer- ous ion transport enzymes and ion transporters associ- ated with osmoregulation were identified in the three osmotic stress groups. Zhu et al found that the NKA α 1 mRNA expression levels in the gills of tilapia reached peak level at 24 h after transfer from freshwater to seawater [45].

Abiotic stress: A constraint in farming

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Heat stress degrades meiosis, decreased pollen germination and growth of the pollen tube, reduced viability, stigmatic and style anomalies, reduced stigmatic pollen grains, disrupted fertilisation process, endosperm growth barriers and unfertilised embryos.. Osmotic pressure due to salinity increases the osmotic stress in. the plant cells, thereby limiting the plant ability to take water and minerals like K + and Ca 2.

Transcriptome sequencing and whole genome expression profiling of hexaploid sweetpotato under salt stress

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In the current study, the response of leaf tissues was lower dur- ing the first hours of salt stress and there were only four TFs significantly up-regulated with one fold higher than control. This indicate that Xuzi-8 sweet- potato as a tolerant cultivar slightly sensed osmotic stress at the first hour of salt stress.

Impacts of fludioxonil resistance on global gene expression in the necrotrophic fungal plant pathogen Sclerotinia sclerotiorum

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Using this approach combined with RNA sequencing, we aimed to characterise the effects of mutations in osmotic stress response genes on the transcriptional profile of the important agricultural pathogen Sclerotinia sclerotiorum under standard laboratory conditions. Our objective was to understand the impact of disruption of the osmotic stress response on the global transcriptional regulatory network in an important agricultural pathogen..

Genome-wide analysis of MicroRNAmessenger RNA interactome in ex-vivo gill filaments, Anguilla japonica

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MicroRNA- messenger RNA interactome analysis of miR-29b-3p and miR-200b-3p revealed the gene targets are essential for osmotic stress responses.. In response to osmotic stress, a distinct suite of modifications in gill epithelia is activated for functional adaptation, which involves cell prolifera- tion and differentiation, changes in the activities, expres- sions and trafficking of different ion transporters/.

Identification and characterization of HAK/ KUP/KT potassium transporter gene family in barley and their expression under abiotic stress

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Gupta et al. a Expression of HvHAKs in response to salt stress (200 mM NaCl). b Expression of HvHAKs in response to osmotic stress (20% PEG8000). c Expression of HvHAKs in response to potassium deficiency (0.01 mM K. Expression of HvHAKs in different tissues and in response to abiotic stresses. In the current study, 27 HAK genes (HvHAKs) were identified in barley.

Temporal salt stress-induced transcriptome alterations and regulatory mechanisms revealed by PacBio long-reads RNA sequencing in Gossypium hirsutum

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Expression fold change of these DEGs showed both positive and negative responses, highlighting the complex nature of salt stress tolerance mechanisms in cotton.. Conclusion: Collectively, this study provides a good insight into the regulatory mechanism under salt stress in cotton and lays the foundation for further improvement of salt stress tolerance.. Plant salt stress response mechanism is mainly stimulated by osmotic stress and Na + [3].

Genome-wide characterization of the GRF family and their roles in response to salt stress in Gossypium

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For example, the activation of the Arabidopsis stress-responsive gene AtDREB2A, whose expression increases plant tolerance to osmotic stress [23, 24], requires repression of at least one GRF. These resources provide the foundation for identifying the suite of GRF genes in Gossypium and their potential roles as salt stress-related genes. Here, we report our genome-wide analysis of the.

Comprehensive genomic analysis of the DUF4228 gene family in land plants and expression profiling of ATDUF4228 under abiotic stresses

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Under cold and osmotic stress, the expression of the AT1G21010 gene was significantly upregulated in aerial parts (Figs. The ex- pression of the AT1G29195 gene in aerial tissues was upreg- ulated under salt stress and osmotic stress, while its expression was significantly downregulated under cold stress (Figs. These findings indicate that At5G67620 may participate in the movement of the stomata via interacting with AT3G08530..

Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis

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The Dehydration Responsive Element (DRE) cis-acting elements are involved in both osmotic and cold stress induced gene expression. While osmotic stress response involves changes in DREB2 regulon gene expression, DREB1 genes are majorly responsible for mounting the cold stress response in plants [76]. In this study, sig- nificant upregulation of the DREB1 gene cassette prompted us to focus on the DREB regulon genes.

Comparative analysis of drought-responsive and -adaptive genes in Chinese wingnut (Pterocarya stenoptera C. DC)

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The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress – and osmotic stress – responsive gene expression. Identification and expression analysis of the stress resistance gene JrGSTU23 from Juglans regia

Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response in Cleistogenes songorica, a xerophyte perennial desert plant

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Furthermore, 12 conserved DEGs were involved in ‘heat acclimation’, and another 12 were involved in the ‘response to osmotic stress’ (Fig. for example, 16 and 13 con- served DEGs were enriched in the ‘chloroplast envelope’. The KEGG pathway analysis revealed that some con- served DEGs were involved in the biosynthesis of differ- ent metabolites, including lipids and sugars (Fig.

Coding and long non-coding RNAs provide evidence of distinct transcriptional reprogramming for two ecotypes of the extremophile plant Eutrema salsugineum undergoing water deficit stress

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Despite this unusually high tolerance to osmotic stress, MacLeod et al. [17] reported that the Yukon and Shandong Eutrema salsugineum accessions respond differently to a drought treatment that includes two periods of water deficit sepa- rated by a brief recovery period. Plants of the Yukon acces- sion accumulate solutes in response to an initial water deficit and during a second drought treatment the plants retain water content longer and maintain leaf expansion..

Genome-wide survey of sucrose nonfermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress

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Pivotal role of the AREB/ABF- SnRK2 pathway in ABRE-mediated transcription in response to osmotic stress in plants. Cloning and characterization of the SnRK2 gene family from Zea mays. Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of SnRK2.8 in the drought-stress response. Genome-wide identification and characterization of the GmSnRK2 family in soybean.

Identification, characterization and functional analysis of grape (Vitis vinifera L.) mitochondrial transcription termination factor (mTERF) genes in responding to biotic stress and exogenous phytohormone

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Multiple Arabidopsis mTERF genes functions have been tested, for instance, Atm- TERF5, 9, 10 and 11 have functions on the resistance to salt and osmotic stress, and AtmTERF5, 9 and 10 also play roles in responding to ABA regulation [40]. Al- though the Arabidopsis mTERF genes can provide pre- dicted characterization for VvmTERF genes, the functional analysis of VvmTERF genes homologs still need more detailed experimental demonstration..

Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis

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The results showed that MtPHD6 transgene or drought stress modulated expres- sion of many AP2/EREBP (AP2, RAV and CBF/DREB2/. ERF), WRKY, and ZINC FINGER transcription factors (Fig. In this study, both drought stress and MtPHD6 transgene modulated ex- pression of several ZINC FINGER transcription factors (STZ, SZF1, CZF1, etc) (Fig. 2 Expression changes of MtPHD6 under drought stress and osmotic stress tolerance of MtPHD6 transgenic Arabidopsis.

Whole-genome characterization of Rosa chinensis AP2/ERF transcription factors and analysis of negative regulator RcDREB2B in Arabidopsis

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Analysis of the osmotic stress-responsive genes and ABA-related genes in VC and RcDREB2B transgenic lines was performed using RT-qPCR. Fifteen cis- acting regulatory elements were found in RcAP2/ERF roses, including ABRE, LTR, MYB, MYC, and DRE, and were designated to 1.5 kb upstream of the RcAP2/ERF ini- tiation codon. When the cis-regulatory elements were ex- amined they showed, that the promoter sequences of the RcAP2/ERF genes were not uniformly distributed.

New insights into the evolution and functional divergence of the CIPK gene family in Saccharum

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Under salt stress, CIPK21 participates in the regulation of response to osmotic stress in A. the evolution and function of the CIPK gene family, we here analyzed comparative genomics analysis with an emphasis on the functional divergence of the CIPK gene family in Saccharum. In this study, sequence and evolu- tion analysis of the SsCIPK genes were conducted using the available sugarcane genome data [42].

Genomics-assisted prediction of salt and alkali tolerances and functional marker development in apple rootstocks

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Osmotic stress may play an important role in salt, alkali and salt-alkali stress. Osmotic stress is one of the major factor participating in salt and alkali induced stress pathways [58, 59]. In this study, the osmolality of solutions for alkali, salt and salt-alkali treatments increased was significantly higher than the control, osmotic stress may be in- volved in all of the stressful treatments.

De novo transcriptome in roots of switchgrass (Panicum virgatum L.) reveals gene expression dynamic and act network under alkaline salt stress

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Due to the combined effects of osmotic, ionic and sec- ondary stresses, plant growth is severely affected by alka- line salt stress [7].