Tìm thấy 11+ kết quả cho từ khóa "RNA interference"
tailieu.vn Xem trực tuyến Tải xuống
Thus, RNA interference followed by transcriptome-wide gene expression analysis by RNA-seq is a popular technique to study the impact of any particular planarian gene on regeneration. Typically, the removal of ribosomal RNA (rRNA) is the first step of all RNA-seq library preparation protocols. Results: In this study, we describe a workflow for the efficient depletion of rRNA in the planarian model species S.
tailieu.vn Xem trực tuyến Tải xuống
Overexpression and RNA interference of GmSPX- RING1 indicated that GmSPX-RING1 negatively regulated P concentration in transgenic hairy roots.
tailieu.vn Xem trực tuyến Tải xuống
A useful feature of C.elegans is that the function of specific genes can be disrupted by RNA interference (RNAi). The nematode can either be soaked in or injected with a solution of double stranded RNA, the sequence of which is complementary to the sequence of the gene that the researcher wishes to disable. RNA interference (RNAi) in C.elegans can also be done by simply feeding the nematode.
ctujsvn.ctu.edu.vn Xem trực tuyến Tải xuống
RNA can thiệp (RNA interference-RNAi) hiện đang được sử dụng rất phổ biến trong nghiên cứu chức năng gen và ức chế sự sao chép của vi-rút ở người, động vật và thực vật (Haasnoot et al., 2003. RNAi là quá trình sinh học xảy ra trong tế bào được kích hoạt bởi các RNA xoắn kép (double- stranded RNA-dsRNA). Các dòng tế bào côn trùng được sử dụng rất phổ biến cho các nghiên cứu về chức năng của gen (Cherbas et al., 1994, Schneider &.
tailieu.vn Xem trực tuyến Tải xuống
To enable this research many state-of-the-art tools have been established, such as an annotated genome and transcrip- tome [11, 12], efficient transgenesis [12], in situ hybrid- isation (ISH) [7, 13], and gene knock-down through RNA interference (RNAi) [3, 14]. The sperm bristles could thus anchor the sperm in the epithelium of the antrum during this post-copulatory suck behaviour [17].
tailieu.vn Xem trực tuyến Tải xuống
Arthropod genomics research in the United States Department of Agriculture-Agricultural Research Service: applications of RNA interference and CRISPR gene editing technologies in pest control.. Validation of RNA interference in western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults. RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera. ABC transporters - from microorganisms to man.
tailieu.vn Xem trực tuyến Tải xuống
Ý tưởng tạo ra con cái giả bằng cách làm bất hoạt RNA thông tin mã hóa gene tuyến đực insuline–like của tôm càng xanh (Macrobrachium rosenbergii insulin–like androgeneic gland, Mr–IAG) bằng RNA mạch đôi (dsRNA) đã được thực hiện thành công tại quy mô phòng thí nghiệm (Ventura và ctv, 2009).
tailieu.vn Xem trực tuyến Tải xuống
Background: RNA interference (RNAi) regulates gene expression in most multicellular organisms through binding of small RNA effectors to target transcripts. Conclusions: Using a strategy that delineates small RNA processing, we found many transcripts have the potential to enter spider mite RNAi pathways, however, trans-acting RNAs appear very unstable and rare. In animals, small RNAs are separated into three major classes.
tailieu.vn Xem trực tuyến Tải xuống
A key gene of the RNA interference pathway in the black tiger shrimp, Penaeus monodon: identification and functional characterisation of Dicer-1. Gene silencing in the spider mite Tetranychus urticae:. dsRNA and siRNA parental silencing of the distal-less gene. Transovarial silencing of the subolesin gene in three-host ixodid tick species after injection of replete females with subolesin dsRNA.
tailieu.vn Xem trực tuyến Tải xuống
To link bio- informatic measurements of gene expression with func- tional outcomes, we interrogated the function of RDGN genes using parental RNA interference (RNAi) in the spider P. tepidariorum, these genes are known to be expressed in all eye types, in the median eyes only, and in the lateral eyes, respect- ively (Fig.
tailieu.vn Xem trực tuyến Tải xuống
Results: RNA interference-mediated knockdown of the HDAC3 gene affected development resulting in abnormally folded wings in pupae and adults. JH analog, hydroprene, suppressed the expression of HDAC3 in T. The knockdown of HDAC3 during the final instar larval stage resulted in an increase in the expression of genes coding for proteins involved in JH action.
tailieu.vn Xem trực tuyến Tải xuống
Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design. Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach. A robust toolkit for functional profiling of the yeast genome. Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile
tailieu.vn Xem trực tuyến Tải xuống
What value of corresponds to the first zero of the 4-slit interference pattern?. What value of corresponds to the first zero. of the 3-slit interference pattern?. of the 4-slit interference pattern?. General properties of N-Slit Interference. The positions of the principal maxima of the intensity patterns always occur at. is the phase between adjacent slits]
tailieu.vn Xem trực tuyến Tải xuống
Thin-Film Interference. Optical Path Length (OPL) between 2 points A and B. n: refractive index of the medium. Intensity is propotional to the squared amplitude of the light wave.. Wave front is the locus of all adjacent points at which the phase of the wave is the same. A ray is an imaginary line along the direction of travel of the wave.. Optical path length of light rays between two wave fronts is equal.. Relation between Phase difference and Optical Path Difference (OPD).
tailieu.vn Xem trực tuyến Tải xuống
Interference of Light. Review: Lights of Different Colors. Interference is a phenomenon in which two coherent light waves superpose to form a resultant wave of greater or lower amplitude (a) Constructive interference: If a crest of one wave meets a crest of another wave, the resultant intensity increases.. (a) Destructive interference: If a crest of one wave meets a trough of another wave, the resultant intensity decreases.. the phase difference between them must remain constant with time..
tailieu.vn Xem trực tuyến Tải xuống
Another method for RNA-seq of FFPE samples is cDNA hybrid capture using a whole exome DNA probe to hybridize to the total RNA library. For RNA-seq of FFPE samples, rRNA depletion from total RNA is the optimal solution. rRNA depletion protocols could keep as much information as possible from the total RNA. Several studies have shown that FFPE RNA-seq data produced high concordance with RNA-seq results from matched frozen fresh samples [11, 12].
tailieu.vn Xem trực tuyến Tải xuống
Comparison of RNA isolation methods on RNA-Seq: implications for differential. In this study, we examined the effects of RNA isolation method as a possible source of batch effects in RNA- seq design..
tailieu.vn Xem trực tuyến Tải xuống
In our previous work, we carried out transcriptome analysis in tall fescue and small RNA analysis and identified. Both RNA-Seq and small RNA analyses provided rich information for the understanding of the molecular mechanism of tiller- ing development or heat stress response in tall fescue.. Here, we identified 18 miRNAs involved in tall fescue tiller development.
tailieu.vn Xem trực tuyến Tải xuống
RNA-seq: a revolutionary tool for transcriptomics. Design and validation issues in rna-seq experiments. Comprehensive evaluation of differential gene expression analysis methods for RNA-seq data
tailieu.vn Xem trực tuyến Tải xuống
Because of cell free saliva, traces of ribosomal RNA and large fragmental RNA were not included in the RNA sample. The treatment of 1/10 th volume 8M lithium chloride to the aqueous phase may also be responsible for enrichment of small RNA in the form of pellet and separation of larger fragmental RNA collected into the supernatant (Prasanta et al.