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Transcriptome sequencing


Tìm thấy 20+ kết quả cho từ khóa "Transcriptome sequencing"

Biclustering of transcriptome sequencing data reveals human tissue-specific circular RNAs

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Results: In this study, circRNA back-splicing junctions were identified from 465 publicly available transcriptome sequencing samples. The result provides thousands of newly identified human tissue-specific circRNAs.. Conclusions: This result suggests that expression of circRNAs is not prompted by random splicing error but serving molecular functional roles.

Comparative transcriptome analyses of genes involved in sulforaphane metabolism at different treatment in Chinese kale using full-length transcriptome sequencing

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genes involved in sulforaphane metabolism at different treatment in Chinese kale using full-length transcriptome sequencing. Here, we investigated the molecular mechanism related to glucosinolates biosynthesis in Chinese kale using combined NGS and SMRT sequencing..

Dynamic transcriptome sequencing and analysis during early development in the bighead carp (Hypophthalmichthys nobilis)

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Dynamic transcriptome sequencing and analysis during early development in the bighead carp ( Hypophthalmichthys nobilis. Background: Early development is a key process of the life history of fish. However, the relationship between the transcriptome and the dynamic regulation of early development is still uncharacterized in the bighead carp (Hypophthalmichthys nobilis). In the present study, we performed transcriptome analysis of six development stages in H.

Microsatellite development from genome skimming and transcriptome sequencing: Comparison of strategies and lessons from frog species

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Table 2 Assembly and microsatellite loci detection statistics for transcriptome sequencing using Trinity. Amolops mantzorum Quasipaa boulengeri Total number of contigs . Total number of SSRs 958 1554. The total number of identified microsatellite loci was reduced by the decline in the quantity of data. As expected, the number of microsatellite loci was associ- ated with the size of dataset.

Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

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Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under. annotations, the annotation of the ER2566 strain was incomplete, with missing gene names and miscellaneous RNAs, as well as uncorrected annotations of some pseudogenes. Here, we performed a systematic reannotation of the ER2566 genome by combining multiple annotation tools with manual revision to provide a comprehensive understanding of the E.

Ouroboros resembling competitive endogenous loop (ORCEL) in circular RNAs revealed through transcriptome sequencing dataset analysis

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CircNet: a database of circular RNAs derived from transcriptome sequencing data. Identification and characterization of circular RNAs as a new class of putative biomarkers in human blood. Specific identification and quantification of circular RNAs from sequencing data. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs. Tracing the expression of circular RNAs in human pre-implantation embryos..

Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing

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Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera. Cultured pearl surface quality profiling by the Shell matrix protein gene expression in the Biomineralised pearl sac tissue of Pinctada margaritifera. Transcriptome analysis of the painted lady butterfly, Vanessa cardui during wing color pattern development.

Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L

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Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L. Background: Optimum flowering time is a key agronomic trait in Brassica napus .

Enhancement of de novo sequencing, assembly and annotation of the Mongolian gerbil genome with transcriptome sequencing and assembly from several different tissues

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Transcriptome sequencing from the 27 tissues generated 131,845 sequences with a total length of bp. The RNA-seq assembly resulted in 19,737 protein-coding genes with a total length of 29.4 Mbp, which is available in the China Na- tional GeneBank CNSA repository, Accession ID:. The transcriptome data was also used to support the annotation and gene predictions as out- lined below in the methods section (Tables 5 and 6).. Genome annotation.

Transcriptome sequencing and metabolome analysis of food habits domestication from live prey fish to artificial diets in mandarin fish (Siniperca chuatsi)

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To un- cover the molecular mechanism why mandarin fish re- fuses artificial diets, we conducted the transcriptome sequencing and metabolome analysis. The metabolome results showed that the pathways with different metabolites were mostly enriched in the meta- bolic pathways, which were consistent with transcrip- tome sequencing results.

Transcriptome sequencing analysis of maize embryonic callus during early redifferentiation

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(Additional file 2: Figure S2), indicating good repeatabil- ity of the sequencing data.. Reliability validation of DEG expression via qRT-PCR Ten DEGs involved in different biological processes (photosynthesis, plant hormone signal transduction, and protein phosphorylation) were randomly selected for qRT-PCR to validate the reliability of the transcriptome sequencing data.

RNA-Seq analysis of soft rush (Juncus effusus): Transcriptome sequencing, de novo assembly, annotation, and polymorphism identification

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Transcriptome de novo assembly from next- generation sequencing and comparative analyses in the hexaploid salt marsh species Spartina maritima and Spartina alterniflora (Poaceae).. Fox SE, Preece J, Kimbrel JA, Marchini GL, Sage A, Youens-Clark K, Cruzan MB, et al. Sequencing and de novo transcriptome assembly of Brachypodium sylvaticum (Poaceae). Fox SE, Geniza M, Hanumappa M, Naithani S, Sullivan C, Preece J, Tiwari VK, et al.

De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism

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The transcriptome sequencing and analysis provides a strong platform of genomic sequences, to serve biosynthetic pathway and metabolic engineering programs of basic as well as applied research on Aloe vera and catalyze path of its change of use from extracts to molecules.. The figure summarizes the steps undertaken and tools used during Aloe vera transcriptome sequencing.

De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development

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Endo-dormancy-imposed growth arrest is one of the key characteristics preventing tree peony from flowering well. used a subtractive cDNA library and transcriptome sequencing, respectively, to identify key genes associ- ated with the release of dormant buds in tree peony;. PsFT, PsVIN3, PsCO, and PsGA20ox were identified to play import- ant roles in the regulation of re-blooming in tree peony by transcriptome sequencing [16].

Didelphis albiventris: An overview of unprecedented transcriptome sequencing of the white-eared opossum

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Genome sequencing and analysis of the Tasmanian devil and its transmissible cancer. The identification of immune genes in the milk transcriptome of the Tasmanian devil (Sarcophilus harrisii). https://doi.org/10.1038/s . Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach. Draft De novo Transcriptome of the rat kangaroo Potorous tridactylus as a tool for cell biology.

PacBio single molecule long-read sequencing provides insight into the complexity and diversity of the Pinctada fucata martensii transcriptome

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sequencing provides insight into the complexity and diversity of the Pinctada fucata martensii transcriptome. To date, the molecular mechanisms of the growth of this species remain poorly understood. The transcriptome sequencing has been considered to understanding of the complexity of mechanisms of the growth of P.

De novo transcriptome sequencing and analysis of salt-, alkali-, and droughtresponsive genes in Sophora alopecuroides

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Mutation landscape and intra-tumor heterogeneity of two MANECs of the esophagus revealed by multi-region sequencing.

The aquatic animals’ transcriptome resource for comparative functional analysis

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Comparative analysis of the Transcriptome in tissues secreting purple and white nacre in the pearl mussel Hyriopsis cumingii. Development of microsatellite markers for the Korean mussel, Mytilus Coruscus (Mytilidae) using next-generation sequencing.. Elucidation of the molecular envenomation strategy of the cone snail Conus geographus through transcriptome sequencing of its venom duct.

Temporal salt stress-induced transcriptome alterations and regulatory mechanisms revealed by PacBio long-reads RNA sequencing in Gossypium hirsutum

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Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L. De novo transcriptome sequencing and comparative analysis of differentially expressed genes in Gossypium aridum under salt stress. Comparative transcriptome analyses of barley and rice under salt stress. Gene expression profiles during the initial phase of salt stress in rice. Unraveling salt stress signaling in plants.

High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm

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Table 2 Overview of transcriptome and small RNA sequencing in fresh and capacitated boar sperm. Q30, the percentage of the base that the quality value of clean data is greater than or equal to 30.Mapped reads (single-end), the number of bases for compared to the reference genome’ clean reads and the percentage in clean reads..