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Handbook of Microbiological Media, Fourth Edition part 204

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Tartoff-Hobbs IliVeg Broth with Glycerol, 1683. TEP Uric Acid Medium, 1691 Tepidanaerobacter Medium., 1691 Tepidanaerobacter synfroplncus* 1692 Tepidibacter Medium with Peptone. Terrific Broth with lOOmicrogram/ml Ampi- cillin. Terrific Broth with 50 nig/ml Ampicillin.. Thermus l-jihanced Agar with 1% Sodium Chloride, 1745. Trichomonas HiVeg Agar Base with Scrum.. I ryptic Soy Agar with 0.6% Yeast lixtract, 1821. Tryplic Soy Agar with...

Handbook of Microbiological Media, Fourth Edition part 1

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MICROBIOLOGICAL MEDIA Fourth Edition. CRC Press is an imprint of the. Printed in the United States of America on acid-free paper . Handbook of microbiological media / Ronald M. Names of Media ...1. Composition of Media...1. Sources of Media...9. The fourth edition of the Handbook of Microbiological Media includes the formulations and descriptions of 7,080 media used for cultivating microorganisms—more...

Handbook of Microbiological Media, Fourth Edition part 2

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For the enrichment of Legionella species.. For the selection and nutrient supplementation of Staphy- lococcus aureus.. In many media for the study of microorganisms in nature, compounds are included in the media as sole sources of carbon or nitrogen so that only a few types of microorganisms can grow. Selective toxic com- pounds are also frequently used to select for...

Handbook of Microbiological Media, Fourth Edition part 3

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Composition per 10.0mL:. cysteine·HCl·H 2 O solution to distilled/deionized water and bring vol- ume to 10.0mL. 1.0g Preparation of Urea Solution: Add urea to distilled/deionized wa- ter and bring volume to 10.0mL. Preparation of Medium: Aseptically combine 160.0mL of cooled, sterile agar base and 45.9mL of sterile supplement solution. Agar base ...80.0mL Supplement solution ...4.6mL. Composition per 165.0mL:. .34.0mg Preparation...

Handbook of Microbiological Media, Fourth Edition part 4

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ionized water and bring volume to 970.0mL. Acetivibrio cellulolyticus Medium Composition per 1170.0mL:. 0.001g Mineral solution 1...75.0mL Mineral solution 2...75.0mL Cellobiose solution ...50.0mL Trace elements solution ...10.0mL Vitamin solution...10.0mL Reducing agent solution...10.0mL. Composition per 50.0mL:. deionized water and bring volume to 50.0mL. 0.3mg Preparation of Trace Elements Solution : Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Composition per 110.0mL:....

Handbook of Microbiological Media, Fourth Edition part 5

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0.3mg Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Composition per 10.0mL:. 1.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 10.0mL. 0.7g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. Add...

Handbook of Microbiological Media, Fourth Edition part 6

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PPLO broth without Crystal Violet...900.0mL Fresh yeast extract solution...100.0mL. Composition per 900.0mL:. Preparation of PPLO Broth without Crystal Violet: Add components to distilled/deionized water and bring volume to 900.0mL.. Composition per 100.0mL:. 25.0g Preparation of Fresh Yeast Extract Solution : Add the live Bak- er’s yeast to 100.0mL of distilled/deionized water. Acholeplasma Medium (ATCC Medium 1215) Composition per 1020.0mL:. PPLO...

Handbook of Microbiological Media, Fourth Edition part 7

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50.0g Agar. 15.0g Plant hydrolysate. 2.5mg Cycloheximide solution ...10.0mL. Composition per 10.0mL:. 0.025g Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except cycloheximide solution, to distilled/deionized water and bring volume to 990.0mL.. Aseptically add 10.0mL cycloheximide solution. 2.5mg Antibiotic solution ...10.0mL. deionized water and bring volume to 10.0mL. Preparation...

Handbook of Microbiological Media, Fourth Edition part 8

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Distribute 100.0mL into flasks. 0.16g Cycloheximide solution ...10.0mL Selenite solution...10.0mL Crystal Violet (0.1% solution) ...2.0mL Selenite Solution:. Composition per 10.0mL:. 0.02g Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. 0.16g Cycloheximide solution ...10.0mL. Selenite solution...10.0mL Malachite Green (0.1% solution)...5.0mL Yeast extract (1% solution)...1.0mL Selenite Solution:. Agar base ...160.0mL Supplement solution ...45.9mL. Composition per 165.0mL:....

Handbook of Microbiological Media, Fourth Edition part 9

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Solution A ...900.0mL Solution B ...100.0mL Solution A:. Composition per 900.0mL:. 0.4g Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Composition per 100.0mL:. 0.5g Preparation of Solution B: Add 0.5g of Na 2 CO 3 to distilled/deion- ized water and bring volume to 100.0mL. Preparation of Medium: Aseptically combine 900.0mL of solu- tion A...

Handbook of Microbiological Media, Fourth Edition part 10

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Composition per 1090.0mL:. A FeEDTA solution ...160.0mL Preparation of Microelements Stock Solution: Add compo- nents to distilled/deionized water and bring volume to 1090.0mL. Composition per 550.0mL:. A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ized water. Composition per 500.0mL:. 28.0g Preparation of Solution B: Add K 2 HPO...

Handbook of Microbiological Media, Fourth Edition part 11

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5.0g Ampicillin solution ...10.0mL. Composition per 10.0mL:. deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except ampicillin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Aseptically add 10.0mL of sterile ampicillin solution. 3.0g Amygdalin solution ...200.0mL Bromthymol Blue (0.05% solution) ...5.0mL. Composition per 200.0mL:. 10.0g. deionized water and bring volume to 200.0mL. Preparation of...

Handbook of Microbiological Media, Fourth Edition part 12

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Preparation of Medium: Add components, except egg yolk emul- sion, to distilled/deionized water and bring volume to 920.0mL. 1.0mg NaHCO 3 solution ...10.0mL. Composition per 100.0mL:. 10.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 100.0mL. Preparation of Medium: Add components, except NaHCO 3 solu- tion, to distilled/deionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 13

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Anaerovibrio burkinabensis Medium Composition per 1011.0mL:. Solution A ...870.0mL Solution C ...100.0mL Solution D ...10.0mL Solution E (Vitamin solution) ...10.0mL Solution F...10.0mL Solution G ...10.0mL Solution B (Trace elements solution SL-10) ...1.0mL. Composition per 870.0mL:. 1.0mg Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL. Composition per liter:. 2.0mg HCl (25% solution)...10.0mL Preparation of Solution...

Handbook of Microbiological Media, Fourth Edition part 14

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39 Composition per liter:. 40 Composition per liter:. 20.0g Agar. 10.0g Glucose. 10.0g Casein enzymatic hydrolysate. 41 Composition per liter:. 20.0g Sodium citrate. Antibiotic Assay Medium B Composition per liter:. 17.0g Agar. 15.0g Glucose. Antibiotic Assay Medium C Composition per liter:. Antibiotic Assay Medium D Composition per liter:. Antibiotic Assay Medium E Composition per liter:. 10.0g Peptone. Antibiotic Assay Medium...

Handbook of Microbiological Media, Fourth Edition part 15

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10.0g Na 2 HPO 4. 11.0g Sodium acetate. 10.0g Polysorbate 80. Distribute into tubes in 10.0mL volumes. Aolpha Medium Composition per 1041.0mL:. 100.0g Agar. 15.0g MgSO 4 ·7H 2 O. 0.1g Metals solution...20.0mL Phosphate solution ...20.0mL Vitamin solution...1.0mL. 10.0g CaCl 2 ·2H 2 O. Composition per 100.0mL:. 0.018g Preparation of Metals “44”: Add components to distilled/deion- ized water and bring...

Handbook of Microbiological Media, Fourth Edition part 16

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1.8mg Biotin ...1.0mg Calcium pantothenate. 20.0g Preparation of Agarose Solution: Add agarose to distilled/deion- ized water and bring volume to 1.0L. 10.0g NaCl. 20.0g Agar. 10.0g. 150.0g NH 4 Cl. 190.0mg MnCl 2 ·4H 2 O. 100.0mg ZnCl 2. 36.0mg NiCl 2 ·6H 2 O. 24.0mg H 3 BO 3. 62.0mg CuCl 2 ·2H 2 O. Composition per 100.0mL:. 10.0mg...

Handbook of Microbiological Media, Fourth Edition part 17

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Composition per 500.0mL:. 1.52g Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Composition per 250.0mL:. 0.52g FeSO 4 ·7H 2 O...1.0μg ZnSO 4 ·7H 2 O ...1.0μg Preparation of Solution B: Add components to distilled/deionized water and bring volume to 250.0mL. Composition per 200.0mL:. 10.0g Preparation of Solution C: Add glucose to distilled/deionized wa-...

Handbook of Microbiological Media, Fourth Edition part 18

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Composition per 100.0mL:. 0.5g Preparation of Bromthymol Blue Solution: Add Bromthymol Blue to 100.0mL of 0.2N KOH. 20.0g Agar. 15.0g K 2 HPO 4. 10.0g K 2 HPO 4. 2.0mg Bromthymol Blue solution ...5.0mL. Composition per 10.0mL:. 0.5g Ethanol...10.0mL Preparation of Bromthymol Blue Solution: Add Bromthymol Blue to 10.0mL of ethanol. Azospirillum Medium Composition per 950.0mL:. 0.1mg Glucose solution ...25.0mL...

Handbook of Microbiological Media, Fourth Edition part 19

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Composition per 400.0mL:. 1.0g Preparation of Component III: Add components to distilled/de- ionized water and bring volume to 400.0mL. Composition per 20.0mL:. 1.0g Preparation of Ferrous Ammonium Sulfate Solution: Add Fe(NH 4 ) 2 (SO 4 ) 2 to distilled/deionized water and bring volume to 20.0mL. Distribute 5.0mL volumes into tubes under 97% N 2 + 3% H 2 ....