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Handbook of Microbiological Media, Fourth Edition part 20

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Preparation of Medium: Add components, except sodium pyru- vate solution, to distilled/deionized water and bring volume to 900.0mL. 0.01g Ferric ammonium citrate...5.0mg Trace elements solution SL-6 ...3.0mL. 5.0mg Trace elements solution ...3.0mL. Composition per 10.0mL:. 0.25g Preparation of Cysteine Solution: Add L -cysteine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL. 0.25g Preparation of Na 2 S·9H 2...

Handbook of Microbiological Media, Fourth Edition part 21

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Add 1.0g of dried liver concentrate. Distribute into tubes or flasks in 10.0mL volumes. 30.0g Glucose. 0.2g Trace elements ...1.0mL. Preparation of Medium: Add components, except agar, to dis- tilled/deionized water and bring volume to 800.0mL. Add agar to 200.0mL of distilled/deionized water. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 20.0g Yeast extract. 15.0g...

Handbook of Microbiological Media, Fourth Edition part 22

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Composition per 10.0mL:. 0.4g Preparation of L -Cysteine·HCl·H 2 O Solution: Add L - cysteine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL. 0.25g Preparation of Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Add Fe 4 (P 2 O 7 ) 3 ·9H 2 O to distilled/deionized water and bring volume to 10.0mL....

Handbook of Microbiological Media, Fourth Edition part 23

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2.5g Glycerol solution...10.0mL. L- Cysteine·HCl–Na 2 S solution...10.0mL pH 7.4 ± 0.2 at 25°C. Composition per 100.0mL:. 87.0g Preparation of Glycerol Solution: Add glycerol to distilled/de- ionized water and bring volume to 100.0mL. 2.5g Preparation of L -Cysteine·HCl–Na 2 S Solution: Add L - cysteine·HCl to distilled/deionized water and bring volume to 80.0mL.. Bring volume to 100.0mL with distilled/deionized water.....

Handbook of Microbiological Media, Fourth Edition part 24

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15.0g Glucose. 10.0g Proteose peptone No. 10.0g Pancreatic digest of casein. Composition per 170.0mL:. Composition per 10.0mL:. Composition per 100.0mL:. 10.0g KH 2 PO 4. 10.0g Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. 0.2g Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. 15.0g Tryptone. 0.2g Glucose solution...

Handbook of Microbiological Media, Fourth Edition part 25

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10.0g Charcoal, activated. 0.25g Antibiotic inhibitor ...10.0mL. L- Cysteine·HCl·H 2 O solution...10.0mL pH 6.9 ± 0.2 at 25°C. Composition per 10.0mL:. 80,000U Preparation of Antibiotic Inhibitor: Add components to dis- tilled/deionized water and bring volume to 10.0mL. cysteine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except antibiotic inhib- itor and L- cysteine·HCl·H...

Handbook of Microbiological Media, Fourth Edition part 26

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Brain Heart Infusion Agar (BAM M24 Medium 2) Composition per liter:. Brain Heart Infusion Agar 0.7%. 10.0g Agar. Distribute into tubes in 25.0mL volumes. 10.0g Plant infusion. 2.0g Penicillin solution ...2.0mL. Composition per 2.0mL:. 0.1g Preparation of Penicillin Solution: Add penicillin to ethanol and bring volume to 2.0mL. Aseptically add 2.0mL penicillin solution. 2.5g Sheep blood, defibrinated ...50.0mL Chloramphenicol solution...10.0mL....

Handbook of Microbiological Media, Fourth Edition part 27

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Preparation of Selective Supplement Solution: Add compo- nents to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except selective sup- plement solution, to distilled/deionized water and bring volume to 990.0mL. 18.0g CaCl 2 ·2H 2 O. 25.0g Glucose. 20.0g CaCO 3. 20.0g Yeast extract. 10.0g Preparation of Medium: Add components to distilled/deionized wa- ter and bring...

Handbook of Microbiological Media, Fourth Edition part 28

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3.0g Bromcresol Purple solution ...2.0mL. Composition per 10.0mL:. 0.16g Ethanol (95% solution) ...10.0mL Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of ethanol. 30.0g Bromcresol Purple solution ...2.0mL. In a separate flask, add glucose to distilled/deionized water and bring volume to 200.0mL. In a third flask, add agar to distilled/deionized water and bring volume to 800.0mL. 80.0g Bromcresol...

Handbook of Microbiological Media, Fourth Edition part 29

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Composition per 100.0mL:. 0.01g NaOH (0.002% solution) ...100.0mL Preparation of Hemin Solution: Add hemin to 100.0mL of NaOH solution. 2.5g Preparation of L -Cysteine·HCl–Na 2 S Solution: Add L - cysteine·HCl to distilled/deionized water and bring volume to 80.0mL.. Bring volume to 100.0mL with distilled/deionized water.. deionized water and bring volume to 100.0mL. Acetic acid ...36.0mL. DL- α-Methylbutyric acid...2.0mL Isovaleric...

Handbook of Microbiological Media, Fourth Edition part 30

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Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 12.0g Sodium pyruvate. 0.001g Selective supplement solution ...10.0mL. Composition per 10.0mL:. 5.0mg Preparation of Selective Supplement Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except selective sup- plement solution, to distilled/deionized water and bring volume to 990.0mL. Aseptially add...

Handbook of Microbiological Media, Fourth Edition part 31

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Composition per 10.0mL:. 1.0g Preparation of Yeast Extract Solution: Add yeast extract to dis- tilled/deionized water and bring volume to 10.0mL. Distribute into anaerobe tubes or bottles containing sulfur, 50.0mg sulfur per 5.0mL medium. Before use in- ject for every 10.0mL medium, 0.05mL sterile vitamin solution, 0.05mL sterile yeast extract, and 0.075mL sterile Na 2 S·9H 2 O solution.. 5.0g...

Handbook of Microbiological Media, Fourth Edition part 32

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2.5g Horse blood, lysed ...50.0mL Selectrive supplement solution ...10.0mL. Composition per 10.0mL:. 2500 U Preparation of Selective Supplement Solution: Add compo- nents to distilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except horse blood and selective supplement, to distilled/deionized water and bring volume to 940.0mL. Aseptically add 50.0mL of lysed horse blood and 10.0mL of sterile...

Handbook of Microbiological Media, Fourth Edition part 33

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20.0mg Ferric ammonium citrate...1.2mg TS2 trace elements solution...1.0mL TS2 Trace Elements Solution:. 20.0g MgSO 4 ·7H 2 O. 0.5mg Vitamin solution...10.0mL NaHCO 3 solution ...10.0mL Na 2 S·9H 2 O solution...10.0mL. L -Cysteine solution ...10.0mL Pyruvate solution ...10.0mL Trace elements solution SL-10 ...1.0mL. Composition per 10.0mL:. 2.5g Preparation of Pyruvate Solution: Add pyruvate to distilled/de- ionized water and bring volume...

Handbook of Microbiological Media, Fourth Edition part 34

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Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). 10.0g NaCl. 0.05g Blood...50.0mL Water-lysed blood solution ...1.5mL. Composition per 8.0mL:. Blood...2.0mL Preparation of Water-Lysed Blood Solution: Add blood to dis- tilled/deionized water and bring volume to 8.0mL. 25.0g NaNO 3. Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of...

Handbook of Microbiological Media, Fourth Edition part 35

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Preparation of Crystal Violet Solution: Add Crystal Violet to 10.0mL of distilled/deionized water. Composition per 10.0mL. 15.0mg Preparation of Cefsulodin Solution: Add cefsulodin to 10.0mL of distilled/deionized water. 2.5mg Preparation of Novobiocin Solution: Add novobiocin to 10.0mL of distilled/deionized water. 1.0g Preparation of Strontium Chloride: Add strontium chloride to 10.0mL of distilled/deionized water. Preparation of Medium: Add 1.0mL irgasan solution...

Handbook of Microbiological Media, Fourth Edition part 36

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0.01g PPLO broth without Crystal Violet...90.0mL Swine serum, inactivated ...10.0mL Phenol Red (1% solution) ...0.25mL. Composition per 500.0mL:. Preparation of PPLO Broth without Crystal Violet: Add components to distilled/deionized water and bring volume to 500.0mL.. 15.0g. 10.0g K 2 HPO 4. Add 25.0mL of medium per Petri dish.. 10.0g Soluble starch. 10.0g NaCl. 10.0g Peptone. 10.0g Starch. 3.5g Sheep blood,...

Handbook of Microbiological Media, Fourth Edition part 37

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Composition per 100.0mL:. 5.0g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 100.0mL. 5.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 100.0mL. 2.0mg Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis- tilled/deionized water...

Handbook of Microbiological Media, Fourth Edition part 38

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Chopped Meat Broth 365. Distribute supernatant into 125.0mL flasks in 10.0mL volumes. Adjust the volume of each flask to 20.0mL with distilled/deionized water. 10.0g K 2 HPO 4. Add 10.0mL of broth to each tube.. Chopped Meat Agar Composition per liter:. 1.0mg NaOH (1N solution)...25.0mL. Add ground meat and 25.0mL of NaOH solution to distilled/deionized water and bring volume to...

Handbook of Microbiological Media, Fourth Edition part 39

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15.0g NaCl. 14.0g NaCl. 0.012g Urea solution...100.0mL. Composition per 100.0mL. 20.0g Preparation of Urea: Add urea to 100.0mL of distilled/deionized wa- ter. Preparation of Medium: Add components, except urea solution, to distilled/deionized water and bring volume to 900.0mL. Aseptically add 100.0mL of sterile urea solution. 20.0g Agar. 15.0g KH 2 PO 4. 20.0g Preparation of Urea: Add urea to 100.0mL...