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Handbook of Microbiological Media, Fourth Edition part 165

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Composition per 100.0mL:. 3.0g Preparation of Calcium Chloride Solution: Add CaCl 2 ·2H 2 O to distilled/deionized water and bring volume to 100.0mL. 15.0g Cornstarch. 10.0g Meat peptone. 10.0g Bromcresol Purple solution ...1.2mL. Composition per 10.0mL:. Solution 1...200.0mL Solution 2...20.0mL. Composition per 200.0mL:. Heart infusion agar ...5.0mL Bromcresol Purple solution ...0.2mL Preparation of Solution 1: Add components to distilled/deionized water...

Handbook of Microbiological Media, Fourth Edition part 166

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Composition per 100.0mL:. 0.1g Preparation of Trace Salts Solution: Add components to dis- tilled/deionized water and bring volume to 100.0mL. 20.0g Glucose. 10.0g Beef extract. 10.0g Glucose. 25.0g Glucose. 10.0g. 1.0g Liver extract...100.0mL. 15.0g. Solution B ...500.0mL Solution A ...400.0mL Solution C ...100.0mL Solution A:. Composition per 400.0mL:. 20.0g Agar. 0.012g Preparation of Solution A: Add components to distilled/deionized water...

Handbook of Microbiological Media, Fourth Edition part 168

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Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Add components, except NaHCO 3 solution, Na 2 S·9H 2 O solution, cysteine solution, NaNO 3 solution, Na-lactate solution, and vitamin solution, to distilled/deionized water and bring volume to 1.0mL. Dispense either 10.0mL aliquots into 15mL Hungate tubes or 50.0mL aliquots into 100mL...

Handbook of Microbiological Media, Fourth Edition part 169

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Solution C ...10.0mL Solution D ...10.0mL Sodium laurate solution ...10.0mL CaCl 2 ·2H 2 O solution...10.0mL. Composition per 916.0mL:. 1.0mg Mineral solution...50.0mL Rumen fluid, clarified ...50.0mL Vitamin solution...5.0mL Trace elements solution SL-10 ...1.0mL Mineral Solution:. 0.01g Preparation of Mineral Solution : Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. deionized water and bring volume to 1.0L. 2.0mg HCl (25% solution)...10.0mL...

Handbook of Microbiological Media, Fourth Edition part 170

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Preparation of Bovine Albumin Solution: Add bovine albumin to distilled/deionized water and bring volume to 100.0mL. Add 50.0mL sterile glucose solution and 50.0mL bovine albumin solution. 0.5g Glucose solution ...50.0mL Bovine serum ...50.0mL. Composition per 100.0mL:. 10.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Add 50.0mL sterile glu- cose solution and 50.0mL...

Handbook of Microbiological Media, Fourth Edition part 171

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10.0g Yeast extract. 25.0mg TTC solution ...3.0mL. Composition per 100.0mL:. 1.0g Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 100.0mL. Preparation of Medium: Add components to distilled/deionized water and bring volume to 997.0mL. Aseptically add 3.0mL of sterile TTC solu- tion. 0.025g TTC solution ...5.0mL. 0.05g Preparation of TTC Solution: Add triphenyltetrazolium chloride to...

Handbook of Microbiological Media, Fourth Edition part 172

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Thauera aromatica AR-1 Medium (DSMZ Medium 855) Composition per 992.0mL:. Solution A ...870.0mL Solution C ...100.0mL Solution D ...10.0mL Solution E (Vitamin solution) ...10.0mL Solution B (Trace elements solution SL-10) ...1.0mL Selenite-tungstate solution...1.0mL. Composition per 870.0mL:. 1.0mg Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL. 2.0mg HCl (25% solution)...10.0mL Preparation of Solution B (Trace...

Handbook of Microbiological Media, Fourth Edition part 173

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0.5mg Glucose solution ...25.0mL Na 2 S·9H 2 O solution ...15.0mL Vitamin solution...10.0mL Yeast extract solution ...5.0mL Trace elements solution SL-10 ...1.0mL. Glucose Solution : Composition per 50.0mL:. 10.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 50.0mL. Yeast Extract Solution : Composition per 10.0mL:. 1.0g Preparation of Yeast Extract Solution: Add yeast extract...

Handbook of Microbiological Media, Fourth Edition part 174

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Na 2 S·9H 2 O solution ...10.0mL Na 2 S 2 O 4 solution...10.0mL Trace elements solution SL-10 ...1.0mL NaHCO 3 solution. Composition per 20.0mL:. 1.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 20.0mL. Composition per 10.0mL:. 0.15g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2...

Handbook of Microbiological Media, Fourth Edition part 175

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Composition per 10.0mL:. 5.0g Preparation of Glucose Solution : Add glucose to distilled/deion- ized water and bring volume to 10.0mL. 1.0g Preparation of Yeast Extract Solution : Add yeast extract to dis- tilled/deionized water and bring volume to 10.0mL. Preparation of Medium: Add components, except glucose solu- tion and yeast extract solution, to distilled/deionized water and bring volume to 980.0mL....

Handbook of Microbiological Media, Fourth Edition part 176

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40.0mg Micronutrient solution ...1.0mL Fe-citrate solution ...0.5mL. Preparation of Medium: Add nitrilotriacetic acid to 100.0mL of distilled/deionized water. 40.0mg Phosphate buffer solution ...100.0mL Ferric citrate solution...0.5mL Trace elements solution ...0.5mL Phosphate Buffer Solution:. Composition per 10.0mL:. 24.5mg Preparation of Ferric Citrate Solution: Add ferric citrate to dis- tilled/deionized water and bring volume to 10.0mL. 20.0mg Preparation of Trace Elements Solution:...

Handbook of Microbiological Media, Fourth Edition part 177

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Solution A ...960.0mL Solution D ...20.0mL Solution B ...10.0mL Solution C ...10.0mL. Composition per 960.0mL:. 50.0mg Preparation of Solution A: Add components to distilled/deionized water and bring volume to 960.0mL. Composition per 10.0mL:. 0.5mg Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. 0.45g Preparation of Solution C: Add glucose to distilled/deionized wa- ter and...

Handbook of Microbiological Media, Fourth Edition part 178

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Distribute into flasks or bottles in 100.0mL volumes. 10.0g KH 2 PO 4. 10.0g K 2 HPO 4. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Thiobacillus thioparus II Medium (DSMZ Medium 486) Composition per 1003.0mL:. Solution A...900.0mL Solution B ...100.0mL Vitamin solution...3.0mL. Composition per 900.0mL:. 0.2g Bromcresol Purple (saturated solution) ...2.0mL Trace metals solution...

Handbook of Microbiological Media, Fourth Edition part 179

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17.0g Glucose. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. 17.0g CaCO 3 , chips or powder. 10.0g Glucose. 5.0mg Na 2 CO 3 solution...10.0mL Vitamin K 1 solution ...10.0mL. Composition per 10.0mL:. 1.0g Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3 to distilled/de- ionized water and bring volume to 10.0mL....

Handbook of Microbiological Media, Fourth Edition part 180

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Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Thiosphaera pantotropha Medium Composition per 1001.0mL:. 0.1g Yeast extract solution ...10.0mL Trace elements solution SL-10 ...1.0mL. Composition per 10.0mL:. 1.0g Preparation of Yeast Extract Solution: Add yeast extract to dis- tilled/deionized water and bring volume to 10.0mL. 190.0mg MnCl 2 ·4H 2 O. 100.0mg ZnCl 2. 70.0mg...

Handbook of Microbiological Media, Fourth Edition part 181

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Glutamine solution...6.0mL CaCl 2 ·2H 2 O solution (optional)...2.0mL. Composition per 100.0mL:. 100.0mL of sterile serum. (TC Minimal Medium Eagle Spinner Modified MEM-S) Composition per 1056.0mL:. 50.0mL–100.0mL Glutamine solution...6.0mL. Tissue Culture Tyrode Solution (TC Tyrode Solution) Composition per 1002.0mL:. 0.05g Phenol Red (1% solution)...2.0mL. Add 2.0mL of Phenol Red solution.. 20.0g KH 2 PO 4. Composition per 20.0mL:. 1.0g Preparation...

Handbook of Microbiological Media, Fourth Edition part 182

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0.15g FeCl 3 ...1.0μg Tween™ 80 ...1.0mL. 5.0g Glucose solution ...50.0mL Glucose Solution:. Composition per 100.0mL:. 20.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Preparation of Medium: Add components, except glucose solu- tion, to distilled/deionized water and bring volume to 950.0mL. Aseptical- ly add 50.0mL of sterile glucose solution. Composition per 730.0mL:....

Handbook of Microbiological Media, Fourth Edition part 183

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15.0g Plant peptone. 3.0g Tributyrin (glyceryl tributyrate)...10.0mL. Preparation of Medium: Add components to distilled/deionized water and bring volume to 990.0mL. Add 10.0mL of tributyrin. 10.0g Na 2 SO 4. 0.7g Glucose solution ...30.0mL. Composition per 30.0mL:. 3.0g Preparation of Glucose Solution : Add glucose to distilled/deion- ized water and bring volume to 30.0mL. Preparation of Medium: Add components, except glucose...

Handbook of Microbiological Media, Fourth Edition part 184

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Preparation of Medium: Add components, except horse serum, to 990.0mL seawater. Aseptically add 10.0mL of sterile horse serum. Trypticase™ Soy Agar (ATCC Medium 18) Composition per liter:. Trypticase™ Soy Agar (Tryptic Soy Agar) (Soybean Casein Digest Agar). Trypticase™ Soy Agar with Cefazidime (ATCC Medium 1997). 5.0g Ceftazidime solution...10.0mL. Composition per 10.0mL:. 4.0mg Preparation of Ceftazidime Solution: Add ceftazidime to dis-...

Handbook of Microbiological Media, Fourth Edition part 185

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(TSA Blood Agar) (BAM M159) Composition per 1050.0mL:. 15.0g Agar. 15.0g Papaic digest of soybean meal. Trypticase™ Soy Tryptose Broth Composition per liter:. Trypticase™ Soy Yeast Extract Agar Composition per liter:. 28.0g NaCl. 10.0g Pancreatic digest of casein. Composition per liter:. Composition per 100.0mL:. deionized water and bring volume to 100.0mL. Composition per 5.0mL:. 43.0g KH 2 PO 4. 17.0g...