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Chủ đề : Genotyping-by-sequencing


Có 15+ tài liệu thuộc chủ đề "Genotyping-by-sequencing"

Genome wide association mapping for heat tolerance in sub-tropical maize

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Results: An association mapping panel, consisting of 662 doubled haploid (DH) lines, was evaluated for yield contributing traits under normal and natural heat stress conditions. Genome wide association studies (GWAS) carried out using 187,000 SNPs and 130 SNPs significantly associated for grain yield (GY), days to 50% anthesis (AD), days to 50% silking (SD), anthesis-silking interval (ASI), plant height (PH),...

Characterization of a haplotype-reference panel for genotyping by low-pass sequencing in Swiss Large White pigs

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Using this approach, we selected 70 key ancestors from two lines of the Swiss Large White breed that have been selected divergently for fertility and fattening traits and sequenced their genomes with short paired-end reads.. Results: Using pedigree records, we estimated the effective population size of the dam and sire line to 72 and 44, respectively. The boars explained 87.95...

Genome-wide association analysis reveals genetic variations and candidate genes associated with salt tolerance related traits in Gossypium hirsutum

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Genome-wide association analysis reveals genetic variations and candidate genes associated with salt tolerance related traits in Gossypium hirsutum. It is therefore important to breed salt tolerance varieties which can help accelerate the improvement of cotton production. The development of molecular markers linked to causal genes has provided an effective and efficient approach for improving salt tolerance.. Results: In this study,...

Evaluation of the efficiency of genomic versus pedigree predictions for growth and wood quality traits in Scots pine

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Evaluation of the efficiency of genomic. Full list of author information is available at the end of the article. [16], and recently, the first draft of the Pinus radiata D. On the other hand, EBV can be estimated by replacing the NRM in the BLUP analysis with a genomic realized relationship matrix (GRM or RRM), generated with the kinship coefficients...

Genetic mapping for agronomic traits in a MAGIC population of common bean (Phaseolus vulgaris L.) under drought conditions

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Background: Common bean is an important staple crop in the tropics of Africa, Asia and the Americas. Founder haplotypes in the population were identified using Genotyping by Sequencing (GBS). No major population structure was observed in the population. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in...

Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants

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Background: The effective use of mutant populations for reverse genetic screens relies on the population-wide characterization of the induced mutations. Genome- and population-wide characterization of the mutations found in fast neutron populations has been hindered, however, by the wide range of mutations generated and the lack of affordable technologies to detect DNA sequence changes. Our GBS pipeline detected 55 of...

Pea genomic selection for Italian environments

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Background: A thorough verification of the ability of genomic selection (GS) to predict estimated breeding values for pea (Pisum sativum L.) grain yield is pending. Results: Some 306 genotypes belonging to three connected RIL populations derived from paired crosses between elite cultivars were genotyped through genotyping-by-sequencing and phenotyped for grain yield, onset of flowering, lodging susceptibility, seed weight and winter...

Translocation of a parthenogenesis gene candidate to an alternate carrier chromosome in apomictic Brachiaria humidicola

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In this study, we used a mapping population of the distantly related commercial species B. High resolution molecular karyotyping indicated that the six homologous chromosomes of the sexual female parent paired at random, whereas preferential pairing of subgenomes was observed in the apomictic male parent. The ASGR-BBML specific PCR product cosegregated with the ASGR in the F 1 mapping population,...

Facilitating population genomics of nonmodel organisms through optimized experimental design for reduced representation sequencing

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To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.. Here, we follow the reasoning of Campbell et al. Ostracoda Macrocyprididae Macroscapha opaca-tensa species complex Brandão et al . Macrocyprididae: Jeffery et al ....

A high-density genetic map of extra-long staple cotton (Gossypium barbadense) constructed using genotyping-bysequencing based single nucleotide polymorphic markers and identification of fiber traits-related QTL in a recombinant inbred line population

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A high-density genetic map of extra-long staple cotton ( Gossypium barbadense). A high-density genetic map with 26 linkage groups (LGs) was constructed using 3557 GBS SNPs spanning a total genetic distance of 3076.23 cM at an average density of 1.09 cM between adjacent markers. A total of 42 QTLs were identified, including 24 QTLs on 12 LGs for fiber quality...

Genomic preselection with genotyping-bysequencing increases performance of commercial oil palm hybrid crosses

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Genotyping-by-sequencing (GBS) yielded +5000 single nucleotide polymorphisms (SNPs) on the parents of the crosses. The genomic best linear unbiased prediction method gave genomic predictions using the SNPs of the training and validation sets and the phenotypes of the training crosses. The practical impact was illustrated by quantifying the additional bunch production of the crosses selected in the validation experiment if...