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Chủ đề : Differentially expressed genes


Có 40+ tài liệu thuộc chủ đề "Differentially expressed genes"

BALLI: Bartlett-adjusted likelihood-based linear model approach for identifying differentially expressed genes with RNA-seq data

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differentially expressed genes with RNA-seq data. Background: Transcriptomic profiles can improve our understanding of the phenotypic molecular basis of biological research, and many statistical methods have been proposed to identify differentially expressed genes (DEGs) under two or more conditions with RNA-seq data. However, statistical analyses with RNA-seq data are often limited by small sample sizes, and global variance estimates of...

Prediction of hub genes associated with intramuscular fat content in Nelore cattle

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Background: The aim of this study was to use transcriptome RNA-Seq data from longissimus thoracis muscle of uncastrated Nelore males to identify hub genes based on co-expression network obtained from differentially expressed genes (DEGs) associated with intramuscular fat content.. From the co-expression network, four modules were identified. The topological properties of the network were analyzed. those genes engaging in the...

Identification and differential expression analysis of anthocyanin biosynthetic genes in leaf color variants of ornamental kale

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These findings enhance the understanding of the genetic mechanisms and regulatory network of anthocyanin accumulation in B. China, Beijing 100097, China Full list of author information is available at the end of the article. In the model plant Arabidopsis thaliana , the biosynthesis, regulation, and transport of anthocyanins, specifically the majority of the structural and regulatory genes in- volved in...

What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization

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Initial development of the embryo relies on maternally- inherited molecules, such as transcripts, deposited in the egg during its formation. We aimed at drawing a molecular portrait of the egg in order to characterize the relation between egg transcriptome and developmental success and to subsequently identify new candidate genes involved in fertility.. The identity and function of differentially expressed genes...

Transcriptome analysis of virulencedifferentiated Fusarium oxysporum f. sp. cucumerinum isolates during cucumber colonisation reveals pathogenicity profiles

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cucumerinum (Foc), is one of the most notorious diseases in cucumber production. Conclusions: By a comparative transcriptome analysis of the mildly and highly virulent strains of Foc during infection of cucumber, a series of DEGs were identified that may be associated with virulence. Monoculture of resistant germplasm may promote in- creased selection for virulence and the directed evolu- tion process...

Differential expression profiling of ΔlitR and ΔrpoQ mutants reveals insight into QS regulation of motility, adhesion and biofilm formation in Aliivibrio salmonicida

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However, little is known about either genes or detailed mechanisms involved in the regulation of these phenotypes.. Thirty-six downregulated genes were gene clusters encoding biosynthesis of the flagellar and chemotaxis genes. Furthermore, the comparison of the transcriptome profiles of Δ litR and Δ rpoQ mutants, exposed numerous overlapping DEGs that were essential for motility, exopolysaccharide production via syp operon and...

Transcriptome profiling of anthocyanin biosynthesis in the peel of ‘Granny Smith’ apples (Malus domestica) after bag removal

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Transcriptome profiling of anthocyanin biosynthesis in the peel of ‘ Granny Smith ’ apples ( Malus domestica ) after bag removal. Results: The anthocyanin content in apple peel increased considerably after bag removal, while only trace amounts of anthocyanins were present in the peel of unbagged and bagged fruits. The − 2026 to − 1870 bp and − 1062 to...

Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum

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In the early stages of this disease, stems show little spots and a loss of green color. However, a lack of transcriptomic and genomic information hinders our understanding of the molecular mechanisms underlying the pitaya defense response.. Conclusions: This study provides a basis for further in-depth research on the protein function of the annotated unigene assembly with cDNA sequences.. 1...

Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.)

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which is one of the major vegetables in China. Cytoplasmic male sterility (CMS) has been used for Wucai breeding in recent years. In this study, the phenotypic and cytological features of Wucai CMS were observed by anatomical analysis, and a comparative transcriptome analysis was carried out to identify genes related to male sterility using Illumina RNA sequencing technology (RNA-Seq).. Results:...

Transcriptional profiling reveals changes in gene regulation and signaling transduction pathways during temperature stress in wucai (Brassica campestris L.)

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Conversely, high-temperature stress inhibited the expression of pivotal genes and weakened the self-regulating ability of the plants.. Full list of author information is available at the end of the article Yuan et al. https://doi.org/10.1186/s . Elucidating the regulatory pattern of the re- sponse of wucai to LT is thus of great interest. After 3d of LT treatment, there was no...

Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7–2 in Wucai (Brassica campestris L.)

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The content of related sugars in the flower buds of MS7 – 2 was significantly lower than that of MF7 – 2, and a large amount of reactive oxygen species (ROS) was accumulated. As an important autumn and winter vegetable, wucai is widely planted in the Yangtze-Huai River Basin.. Stamen development plays an important role in the sexual reproduction of...

Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress

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provides insight into gene expression dynamics during continuous salt stress. However, understanding of the salt-adaptation mechanism of this species is limited because of genomic sequences scarcity. Most DEGs were activated within 6 h of salt stress and their expression stabilized after 48 h. the number of DEGs was greatest within 12 h of salt stress. Gene annotation and functional analyses...

Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

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Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs. Results: To unveil conserved Cd-responsive genes in cereal plants, the obtained 5166 maize DEGs were compared with 2567 Cd-regulated orthologs in rice roots, and this comparison generated 880 universal Cd-responsive orthologs groups composed of 1074 maize DEGs and 981 rice counterparts. More importantly, most of the orthologous DEGs...

Study on differentially expressed genes related to defoliation traits in two alfalfa varieties based on RNA-Seq

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Study on differentially expressed genes related to defoliation traits in two alfalfa varieties based on RNA-Seq. Thus far, the understanding of the molecular mechanism of alfalfa defoliation traits remains unclear. The transcriptome database created by RNA-Seq is used to identify critical genes related to defoliation traits.. Results: In this study, we sequenced the transcriptomes of the Zhungeer variety (with easy...

Corn dried distillers grains with solubles (cDDGS) in the diet of pigs change the expression of adipose genes that are potential therapeutic targets in metabolic and cardiovascular diseases

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Corn dried distillers grains with solubles (cDDGS) in the diet of pigs change the expression of adipose genes that are. However, feed mixtures too high in cDDGS result in the worsening of backfat quality. The aim of the present experiment was to evaluate changes in the backfat transcriptome of pigs fed isoenergetic diets that differed in cDDGS presence.. 0.00189), fatty...

Transcriptome dynamics associated with resistance and susceptibility against fusarium head blight in four wheat genotypes

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In the three resistant genotypes, 220 DEGs were associated with resistance.. In the pathogen, most genes showed increased expression between 2 and 4 dpi in all genotypes, with stronger levels in the susceptible host. A minor QTL associated with type II resistance was also identified by the same authors in the Chinese genotype Wuhan 1 on chromosome 2DL.. They also...

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

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Background: The Mediterranean diet is considered one of the healthier food habits and olive oil is one of its key components. A morphological analysis of the jejunum revealed that OMWW reduced crypt depth, whereas no significant modifications were observed for villus height and the villus height/crypt depth ratio. An enrichment analysis revealed that the majority of up regulated genes in...

Transcriptomic analysis of genes in soybean in response to Peronospora manshurica infection

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Transcriptomic analysis of genes in soybean in response to Peronospora manshurica infection. To date, little is known regarding the defense mechanism at molecular level and how soybean plants response to Pm infection. The yield loss could be as high as 6–15% on average in the years when it was epidemic [1–4]. Full list of author information is available at the...

Genome-wide expression profiling of leaves and roots of watermelon in response to low nitrogen

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Genome-wide expression profiling of leaves and roots of watermelon in response to. The N concentration in the leaf, stem, and root of watermelon under low N conditions was reduced by 68, 104, and 108%, respectively compared with 9 mM N treatment after 14 days of N treatment. In the leaf tissues of watermelon grown under low N conditions, 9598 genes...

Transcriptome analysis of chrysanthemum (Dendranthema grandiflorum) in response to low temperature stress

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LTPs c98772.graph_c0 2.06 up 2.26 up 0.38 normal Non-specific lipid transfer protein. c90293.graph_c0 4.24 up 4.30 up 1.47 normal Non-specific lipid-transfer. c86130.graph_c0 4.39 up 4.66 up 1.53 normal Lipid transfer-like protein. c96305.graph_c0 1.50 normal 2.41 up 1.28 normal Non-specific lipid-transfer. c45235.graph_c0 3.55 up 2.77 normal 3.83 up Putative lipid-transfer protein. c92863.graph_c0 2.98 normal 3.81 up. c98666.graph_c0 3.35 up 2.63 up...