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Chủ đề : Transcriptome analysis


Có 20+ tài liệu thuộc chủ đề "Transcriptome analysis"

Transcriptome analysis of ovary tissues from low- and high-yielding Changshun green-shell laying hens

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Transcriptome analysis of ovary tissues from low- and high-yielding Changshun green-shell laying hens. Improving egg production performance has become an important breeding task, and in recent years, the development of high-throughput sequencing technology provides a fast and exact method for genetic selection.. Therefore, we aimed to use this technology to analyze the differences between the ovarian mRNA transcriptome of low...

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

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alteration of the structures and gene expression profiles of the midgut. frugiperda larvae.. Histological and cytological changes were examined in the midgut of larvae fed on an artificial diet supplemented with 1.0 and 5.0 µg/g CPT. A total of 915 and 3560 differentially expressed genes (DEGs) were identified from samples treated with 1.0 and 5.0 µg/g CPT, respectively.. The images...

Transcriptome analysis of embryonic muscle development in Chengkou Mountain Chicken

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Transcriptome analysis of embryonic muscle development in Chengkou Mountain Chicken. The embryonic period is highly critical for muscle development, the number, shape and structure of muscle fibers are determined at the embryonic stage. Herein, we performed transcriptome analysis to reveal the law of muscle development in the embryonic stage of Chengkou Mountain Chicken at embryonic days (E . Results: Diameter...

Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker

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Transcriptomic analysis of s-methoprene resistance in the lesser grain borer,. The number of produced progeny decreased in presence of the inhibitor, especially in the resistant strain. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Full list of author information is...

GCSscore: An R package for differential gene expression analysis in Affymetrix/ Thermo-Fisher whole transcriptome microarrays

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However, the prior version of the Sscore algorithm and a R-based implementation software, sscore , do not function with the latest generations of Affymetrix/Fisher microarrays due to changes in microarray design that eliminated probes previously used for estimation of non-specific binding.. Results: Here we describe the GCSscore algorithm, which utilizes the GC-content of a given oligonucleotide probe to estimate non-specific...

Simultaneous changes in anthocyanin, chlorophyll, and carotenoid contents produce green variegation in pink–leaved ornamental kale

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produce green variegation in pink – leaved ornamental kale. Ornamental kale (Brassica oleracea var. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted...

Comprehensive transcriptomic analysis provides new insights into the mechanism of ray floret morphogenesis in chrysanthemum

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provides new insights into the mechanism of ray floret morphogenesis in. Background: The ray floret shapes referred to as petal types on the chrysanthemum (Chrysanthemum × morifolium Ramat.) capitulum is extremely abundant, which is one of the most important ornamental traits of chrysanthemum.. However, the regulatory mechanisms of different ray floret shapes are still unknown. Therefore, it is an ideal...

Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis): Performance of different sexes, candidate genes and networks

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Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis):. Several genes and gene families have been involved in high temperature responses, such as heat shock protein (hsp) family, Jumonji family and genes related to cortisol synthesis. conducted on females, pseudomales and males exposed to high (28 °C) and normal (22 °C) temperatures. Principal component analysis (PCA) and differentially...

Transcriptome analysis of genes and pathways associated with metabolism in Scylla paramamosain under different light intensities during indoor overwintering

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Background: Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan. Light intensity is one of the ecological factors that affect S. The findings of the study add to the knowledge of regulatory mechanisms related to S. paramamosain metabolism under different light intensities.....

Transcriptome analysis provides insights into the non-methylated lignin synthesis in Paphiopedilum armeniacum seed

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Backgrounds: Paphiopedilum is an important genus of the orchid family Orchidaceae and has high horticultural value. Several key genes in the lignin biosynthetic pathway displayed different expression patterns during the lignification process. Conclusions: This work demonstrated the plasticity of natural lignin polymer assembly in seed and provided a better understanding of the molecular mechanism of seed-specific lignification process.. As a...

Investigation into the underlying regulatory mechanisms shaping inflorescence architecture in Chenopodium quinoa

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Unveiling of the regulatory mechanisms underlying inflorescence architecture is pivotal for improving crop yield potential. However, dissection of the regulatory networks underlying quinoa inflorescence patterning is lacking.. The data obtained from this study will enhance our understanding of the gene network regulating quinoa inflorescence architecture, as well will supply with valuable genetic resources for high-yield elite breeding in the future.....

RNA-Seq based transcriptome analysis during bovine viral diarrhoea virus (BVDV) infection

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Background: Bovine viral diarrhoea virus (BVDV) is the member of the genus Pestivirus within the Flaviviridae family and responsible for severe economic losses in the cattle industry. To further understand the mechanisms by which BVDV overcomes the host cell innate immune response and provide more clues for further understanding the BVDV-host interaction, in this descriptive study, we conducted a investigation...

A cmap-enabled gene expression signature-matching approach identifies small-molecule inducers of accelerated cell senescence

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A cmap-enabled gene expression. Results: We examined senescence induced by ionizing irradiation or ectopic overexpression of the stoichiometric cyclin-dependent kinase (CDK) inhibitor p21 CIP/WAF1/SDI1. While radiation produces a strong DNA damage response, ectopic expression of p21 arrests cell cycle progression independently of DNA damage. Systems analysis of the shared differentially expressed genes (DEGs) revealed strong signals for cell cycle control...

Genetic mechanism underlying sexual plasticity and its association with colour patterning in zebrafish (Danio rerio)

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Contrary to expectations, we found highly differential expression of colour pattern (CP) genes in the gonads, suggesting the ‘ neofunctionalisation ’ of those genes in the zebrafish reproduction system. However, in the caudal fin, no differential expression of CP genes was identified, suggesting the observed differences in colouration between males and females in adult fish may be due to post-transcriptional...

Transcriptome analysis of bolting in A. tequilana reveals roles for florigen, MADS, fructans and gibberellins

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Results: Consistent morphological features could not be determined for the vegetative to reproductive transition in A.. tequilana plants from different developmental stages identified OASES as the most effective assembly program and revealed evidence for incomplete transcript processing in the highly redundant assembly obtained. Gene ontology analysis uncovered enrichment for terms associated with carbohydrate and hormone metabolism and detailed analysis of...

Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822

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nicotine degradation in Aspergillus oryzae 112822. In our previous work, we isolated an efficient nicotine-degrading fungus Aspergillus oryzae 112822 and first proposed a novel demethylation pathway of nicotine degradation in fungi. However, the underlying mechanisms of the demethylation pathway remain unresolved. In the present study, we performed a comparative transcriptome analysis to elucidate the molecular mechanisms of nicotine tolerance and...

Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.)

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which is one of the major vegetables in China. Cytoplasmic male sterility (CMS) has been used for Wucai breeding in recent years. In this study, the phenotypic and cytological features of Wucai CMS were observed by anatomical analysis, and a comparative transcriptome analysis was carried out to identify genes related to male sterility using Illumina RNA sequencing technology (RNA-Seq).. Results:...

Screening of Streptococcus Suis serotype 2 resistance genes with GWAS and transcriptomic microarray analysis

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Background: Swine streptococcosis has caused great economic loss in the swine industry, and the major pathogen responsible for this disease is Streptococcus Suis serotype 2 (SS2). With the development of GWAS and transcriptomic microarray technology, we now have powerful research tools to identify SS2 resistance genes.. With the F 2 generation of these two mice strains and GWAS analysis, we...

Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors

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These compounds are widely implicated in the beer brewing industry and are having potential biomedical applications. In the present work, a pioneering attempt has been made to overexpress master regulator binary transcription factor complex formed by HlWRKY1 and HlWDR1 using a plant expression vector to enhance the level of prenylflavonoid and bitter acid content in the hop. The detailed analyses...