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Chủ đề : Single nucleotide polymorphism


Có 20+ tài liệu thuộc chủ đề "Single nucleotide polymorphism"

Integration of Infinium and Axiom SNP array data in the outcrossing species Malus × domestica and causes for seemingly incompatible calls

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Next, the accuracy of the SNP calls was evaluated on additional germplasm (n = 3141) from both arrays using Mendelian inconsistent and consistent errors across thousands of pedigree links. The impact of the latter was highly influenced by their number and proximity to the 3 ′ end of the probe.. Full list of author information is available at the end...

Genome-wide patterns of differentiation within and among U.S. commercial honey bee stocks

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commercial honey bee stocks. honey bee stocks remain poorly characterized despite the agricultural importance of Apis mellifera as the major crop pollinator. The variety of bees produced by artificial selection provides an opportunity to characterize the genetic diversity and regions of the genome undergoing selection in commonly managed stocks.. Results: Pooled sequencing of eight honey bee stocks found strong genetic...

A new method for long-read sequencing of animal mitochondrial genomes: Application to the identification of equine mitochondrial DNA variants

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We sequenced these amplified mitochondrial DNA using nanopore sequencing technology and obtained mitochondrial DNA reads that represented up to half of the sequencing output. The sequence reads were 2.3 kb of mean length and provided an even coverage of the mitochondrial genome. Long-reads spanning half or more of the whole mtDNA provided a coverage that varied between 118X and 488X....

Investigating the accuracy of imputing autosomal variants in Nellore cattle using the ARS-UCD1.2 assembly of the bovine genome

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Investigating the accuracy of imputing autosomal variants in Nellore cattle using the ARS-UCD1.2 assembly of the bovine genome. Background: Imputation accuracy among other things depends on the size of the reference panel, the marker ’ s minor allele frequency (MAF), and the correct placement of single nucleotide polymorphism (SNP) on the reference genome assembly. Further, we compared the reliability of...

Population analysis of the Korean native duck using whole-genome sequencing data

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Population analysis of the Korean native. Duck is one of the most popular domesticated waterfowls, which is economically important as a source of meat, eggs, and feathers. To study the distinct genomic features of Korean native duck, we conducted population-level genomic analysis of 20 Korean native ducks together with 15 other duck breeds.. Results: A total of 15.56 million single...

Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus

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Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus. napus germplasm resource exhibiting up-curled leaves and a semi-dwarf stature.. In progeny populations derived from NJAU5737 and Zhongshuang 11 (ZS11), we found that the up-curled leaf trait was controlled by a dominant locus, BnUC2. This result suggests that the candidate gene mutation (BnaA05g16700D, encoding...

Genome-wide identification and characterization of ABA receptor PYL gene family in rice

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The promoters of most of the OsPYLs have cis-regulatory elements involved in imparting abiotic stress responsive expression. Full list of author information is available at the end of the article. Despite the advancements achieved towards the comprehension of the role of PYL gene family in Arabidopsis, functional diversity and re- dundancy of PYLs in development and stress responsive processes in...

Genome wide association mapping and candidate gene analysis for hundred seed weight in soybean [Glycine max (L.) Merrill]

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Genome wide association mapping and candidate gene analysis for hundred seed weight in soybean [ Glycine max (L.) Merrill]. Background: The hundred seed weight (HSW) is one of the yield components of soybean [ Glycine max (L.) Merrill]. The seed weight (SW) of soybean (often denoted by hundred seed weight (HSW)) is an important yield com- ponent and positively correlates...

Deep expression analysis reveals distinct cold-response strategies in rubber tree (Hevea brasiliensis)

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In this work, transcriptome sequencing was used to identify a full set of transcripts and to evaluate the gene expression involved in the different cold-response strategies of the RRIM600 (cold-resistant) and GT1 (cold-tolerant) genotypes.. Full list of author information is available at the end of the article. Although the Amazon basin offers a suitable climate for this crop, Southeast Asia...

A high-throughput SNP discovery strategy for RNA-seq data

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A high-throughput SNP discovery strategy for RNA-seq data. The rapid advance of next generation sequencing (NGS) provides a high-throughput means of SNP discovery. However, SNP development is limited by the availability of reliable SNP discovery. Especially, the optimum assembler and SNP caller for accurate SNP prediction from next generation sequencing data are not known.. Results: Herein we performed SNP prediction...

Identification of new loci for salt tolerance in soybean by high-resolution genomewide association mapping

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Identification of new loci for salt tolerance in soybean by high-resolution genome- wide association mapping. Although a major gene for salt tolerance was identified and consistently mapped to chromosome (Chr.) 3 by linkage. Salt tolerance among soybean lines was evaluated by leaf scorch score (LSS), chlorophyll content ratio (CCR), leaf sodium content (LSC), and leaf chloride content (LCC).. Results: For...

Genetic and transcriptomic dissection of the fiber length trait from a cotton (Gossypium hirsutum L.) MAGIC population

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Genetic and transcriptomic dissection of the fiber length trait from a cotton. Gossypium hirsutum L.) MAGIC population. Background: Improving cotton fiber length without reducing yield is one of the major goals of cotton breeding.. However, genetic improvement of cotton fiber length by breeding has been a challenge due to the narrow genetic diversity of modern cotton cultivars and negative correlations...

Genome analysis identifies a spontaneous nonsense mutation in ppsD leading to attenuation of virulence in laboratorymanipulated Mycobacterium tuberculosis

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attenuation of virulence in laboratory- manipulated Mycobacterium tuberculosis. Background: A previous laboratory study involving wild type, mutant and devR / dosR complemented strains of Mycobacterium tuberculosis reported the attenuation phenotype of complemented strain, Comp1. This phenotype was intriguing since the parental strain H37Rv, devR mutant (Mut1) and additional complemented strains, Comp9 and Comp11, were virulent in the guinea pig model.....

A catalog of annotated high-confidence SNPs from exome capture and sequencing reveals highly polymorphic genes in Norway spruce (Picea abies)

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Background: Norway spruce [Picea abies (L.) Karst.] is ecologically and economically one of the most important conifer worldwide. The estimated true positive rate of the resource was 84.2%, which was comparable with the genotyping success rate obtained for P. 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0. The Creative...