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Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

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Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs. Background: There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA.

Correction for both common and rare cell types in blood is important to identify genes that correlate with age

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Conclusions: We conclude that gene expression analyses in blood strongly benefit from correction for both common and rare blood cell types, and recommend using blood-cell count estimates as standard covariates when studying whole blood gene expression.. Full list of author information is available at the end of the article.

Ancestry-specific associations identified in genome-wide combined-phenotype study of red blood cell traits emphasize benefits of diversity in genomics

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Ancestry-specific generalization varied by trait, with the highest proportion of generalization oc- curring in the European-ancestry sub-population and the lowest occurring in African Americans, which may be due to power differences to detect associations by ancestry.. 1%) in the European-ancestry GTEx population and had available information in whole blood, liver, spleen, and/or thyroid tissues. 1% in the GTEx study population and hence could not be evaluated for cis-eQTLs..

Đại học Y Dược Tp.HCM Khoa ĐD-KTYH Bộ môn Xét Nghiệm

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IL-6 Nhiệt độ bảo quản. 2 C : HC dễ vỡ tai biến truyền máu 1- Máu toàn phần (WHOLE BLOOD): Chỉ định: Mất máu cấp ( >25% Vmáu BN ml/ BN 50-60kg ) Mất máu < 25% Vmáu (700-800ml) kèm thêm tình trạng. 1- Máu toàn phần (WHOLE BLOOD): Chỉ định: Truyền thay máu (exchange transfusion) trong những trường hợp tán huyết do.

Evaluation of single step TaqMan real-time PCR assay lateral to conventional RT-PCR and antigen-capture ELISA for pre-clinical detection of classical swine fever virus

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Biological samples such as nasal and ocular swabs, tonsillar scrapings and whole blood collected from each animal at pre-clinical and late clinical phase of the disease were processed and tested by single step RT-qPCR, nested RT-PCR and Ag-ELISA.. Haematological analysis was carried out in a total of 70 blood samples collected at pre- clinical (n=30) and late clinical phase (n=30) of the disease. Detection of CSFV antigen and nucleic acid.

Comparative transcriptome analysis reveals ectopic delta-5 and delta-6 desaturases enhance protective gene expression upon Vibrio vulnificus challenge in Tilapia (Oreochromis niloticus)

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For inflammatory factors, cytokines and complement-related genes, we found that were sev- eral significant differences between wild-type and D56- transgenic tilapia whole blood.

DNA methylation of alu repeats in complicated urinary tract infection in children

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The manifestation of DNA methylation in Alu repeats from whole blood was analyzed in UTI cases and HC. Hypomethylation of Alu repeats was observed among UTI cases. Hypermethylation in HC indicates the trend of the presence of active de nova DNA methylation of Alu element than UTI cases (22).. Table.4 Comparison of Percentage of Alu DNA methylation between cases and controls Parameters % of Alu DNA methylation (Mean ±SD) P value.

Section X - Drugs Used for Immunomodulation

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Seven major metabolites have been identified in whole blood (Salm et al. Sirolimus is indicated for prophylaxis of organ transplant rejection in combination therapy with a calcineurin inhibitor and glucocorticoids (Kahan et al. It is recommended that the maintenance dose be reduced by approximately one-third in patients with hepatic impairment (Watson et al.

BC-3600 Vietnames OP

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Ch ọ n bi ể u t ượ ng chuy ể n đổ i mode trên màn hình , máy s ẽ chuy ể n t ừ “Whole blood” sang “Prediluted. Nh ấ n bi ể u t ượ ng b ơ m diluent , máy s ẽ hi ể n th ị thông báo sau.

Genomic targets for high-resolution inference of kinship, ancestry and disease susceptibility in orang-utans (genus: Pongo)

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We supplemented these with 51,128 additional SNP positions derived from 71 zoo-housed orang-utans that we genotyped from whole blood or tissue-derived DNAs on the Illumina iScan platform. We first extracted gen- omic DNA using either the Maxwell RSC Blood DNA or Tissue DNA kits, respectively, as automated on the Table 1 Published, re-sequenced genomes from 37 orang-utans. 0.01 and converted the final GenomeStudio file to VCF using the iScanVCFMerge tool (Fountain et al., in review)..

Quantitative characterization of tumor cellfree DNA shortening

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The log2 fold change ratio of each 100 k bp window was calculated by dividing normalized read count of the tumor sample by that of its matched whole blood control.. CNV analysis of targeted sequencing was performed through a customized algorithm, which is a part of the LDT. The log2 fold change ratio of each target interval was calculated by dividing normalized read count of the cfDNA sample by that of its matched whole blood con- trol.

Pan-tissue transcriptome analysis of long noncoding RNAs in the American beaver Castor canadensis

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We profiled the expression of the 187 high-confidence lncRNAs across 16 beaver tissues (whole blood, brain, lung, liver, heart, stomach, intestine, skeletal muscle, kidney, spleen, ovary, placenta, castor gland, tail, toe-webbing, and tongue) and identified both tissue- specific and ubiquitous lncRNAs..

Blood-based epigenetic estimators of chronological age in human adults using DNA methylation data from the Illumina MethylationEPIC array

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Table 1 Description of the peripheral whole-blood-derived DNAm data on the EPIC platform Cohort Tissue type Platform GEO submitter N Normalization. EPIC Curtis et al. EPIC Shinozaki et al.. EPIC Arpon et al. EPIC Kilaru et al. a Pre-processing method for quantifying DNAm levels in the range of 0 to 1 Noob Normal-exponential out-of-band [29]. However, compared to eABEC, the accuracy of cABEC in the test set was slightly diminished (MAD .

RNA-Seq analysis of blood meal induced gene-expression changes in Aedes aegypti ovaries

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Clearly, a complex series of physiological events occurs in multiple tissues (e.g., midgut, fat body, and ovary) fol- lowing blood meal ingestion. RNA-Seq analysis provides a useful tool to analyze changes in gene expression in the whole organism as well as in pertinent tissues [19, 20]. Comparing gene expression patterns at various time points between sugar-fed and blood fed mosquitoes and tissues, one can identify the organism’s or tissue-specific responses to the blood meal.

Effects of AGXT2 variants on blood pressure and blood sugar among 750 older Japanese subjects recruited by the complete enumeration survey method

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We recruited a total of 927 subjects (61.3% of the whole population aged >. In terms of DDAH1 SNPs and 4 N ins/del, the allele frequencies of these genotypes were also simi- lar to those in the dbSNP database (https://www.ncbi.. However, we have to consider that ADMA concentrations were significantly higher in the GG genotype (gain of func- tion) than in the AA genotype (loss of function) of rs180749.

Section XI - Drugs Acting on the Blood and the Blood-Forming

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The next several years should see a further expansion of the use of growth-regulating agents in the. macromolecules in the subendothelial regions of the injured blood vessel.

Multi-ethnic genome-wide association analyses of white blood cell and platelet traits in the Population Architecture using Genomics and Epidemiology (PAGE) study

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Annotation of the independent variants we identified in the stepwise conditional analysis was performed by extracting information of each available variant from the whole genome sequence annotator (WGSA) dataset (Supplemental Table 12). We also examined these independent variants in the BCX gchromVAR results.

Species and population specific gene expression in blood transcriptomes of marine turtles

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Additionally, this ‘marine turtle core gene set’ contained 84.4% of the genes in the core set across reptilian blood transcriptomes previously identified by Waits et al. Green turtle - blood. The number of genes in each GO slim functional category a from green turtle blood transcriptome genes that belonged to orthogroups present in all four species ’ blood transcriptomes and b multi-tissue leatherback transcriptome genes that belonged to orthogroups present in all four leatherback tissues.

Assessing genomic diversity and signatures of selection in Jiaxian Red cattle using whole-genome sequencing data

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Permission for blood sampling was obtained from the Jiaxian Red Cattle Breeding Center on site.. Summary of the improvement work of breeding and conservation of Jiaxian red cattle (in Chinese). Comprehensive analysis of the mitochondrial DNA diversity in Chinese cattle. Whole-genome

A global analysis of CNVs in Chinese indigenous fine-wool sheep populations using whole-genome resequencing

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We collected blood samples from 32 fine-wool sheep (2- year-old rams, Additional file 14: Table S11), including 16 Alpine Merino sheep (8 horned, AMS_horn. The integrity and purity of the DNA was determined using 1.5% agarose gel electrophoresis and a NanoDrop 2000. CNVnator software (version 0.3) was used to detect the CNVs in the sheep genome samples [31]. The gene copy numbers (CN) of the genomes were estimated using the CNVnator “-geno- type” option.