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Chủ đề : Flow cytometry


Có 11+ tài liệu thuộc chủ đề "Flow cytometry"

TẠO CÂY DƯA HẤU TỨ BỘI BẰNG XỬ LÝ COLCHICINE IN VITRO

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TẠO CÂY DƯA HẤU TỨ BỘI BẰNG XỬ LÝ COLCHICINE IN VITRO. Dưa hấu không hạt được tạo thành do lai giữa cây dưa hấu tứ bội (4X=44) làm mẹ và cây nhị bội (2X=22) làm cha.. Trong tự nhiên, cây dưa hấu tứ bội không hiện diện. Thông thường, cây tứ bội được phát triển bởi xử lý đột...

Application of CD54 in diagnosing bone marrow involvement by using flow cytometry in patients with diffuse large Bcell lymphoma

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Application of CD54 in diagnosing bone marrow involvement by using flow. In this study, we detected the expression CD54 on lymphoma cells in BM specimens from DLBCL patients and clarified its diagnostic significance in BM involvement by DLBCL.. We detected and compared the expression of CD54 on lymphoma cells and normal mature B cells by using 10-color panels.. Results: Normal...

The effect of R547, a cyclin-dependent kinase inhibitor, on hepatocellular carcinoma cell death

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Hepatocellular carcinoma (HCC) is one of the most common types of cancer that originates from hepatocytes.. For approximately 70% of patients with HCC, this period is about 5 years (Wang et al., 2012). Treatment of the disease depends on tumor size and stage, and high-grade tumors result in poor prognosis. Surgery is one of the main treatment methods for HCC....

Genome size variation among natural populations of Brachypodium distachyon and B. hybridum collected from different regions of Turkey

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Genome size variation among natural populations of Brachypodium distachyon and B. Brachypodium distachyon is a useful model organism to study various aspects of plant and grass biology (Vogel et al., 2010. Catalán et al., 2014. Scholthof et al., 2018). Furthermore, it is also advantageous in terms of conversion to cereal and biofuel crops (Catalán et al., 2014. Lopez-Alvarez et al.,...

Genome size confirms the presence of Artemisia verlotiorum (Asteraceae) in Lebanon

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doi:10.3906/bot-2001-36. Although initially described based on specimens collected near Clermont-Ferrand in France in the second half of the 19th century (Lamotte, 1877), Artemisia verlotiorum Lamotte (Asteraceae) is native to China (Ling et al., 2011).. It is reportedly known from Australia and New Zealand, many countries in Europe and Southeastern Asia, and in North and South America (Ling et al., 2011....

Application of structural, functional, fluorescent, and cytometric indicators for assessing physiological state of marine diatoms under different light growth conditions

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Kovalevsky Institute of Biology of the Southern Seas of RAS, Moscow, Russian. Phytoplankton is a basic component of aquatic ecosystems, which determines the productivity of all links of the trophic chain and plays a key role in the global cycles of carbon, nitrogen, and phosphorus. A rapid response of microalgae to changes in environmental conditions allows using changes in their...

Fluorescein labelled annexin V-staining limitations for determination of blastodermal cells apoptosis

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blastodermal cells. Samli et al., 2005. Jin et al., 2011;. Hamidu et al., 2011. Dymond et al., 2013). The BCs are cells occured at egg oviposition (X stage) (Eyal-Giladi, Kochav, 1976) with the ability to contribute to the germline, ectodermal, mesodermal, and endodermal lines (Carsience et al., 1993. Fraser et al., 1993. Thoraval et al., 1994. Kagami et al., 1995;....

Induction of apomixis and fixation of heterosis in Egyptian rice Hybrid1 line using colchicine mutagenesis

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Pollen fertility examination was performed on the populations of the third season. The flow cytometry screening of the six yield-based identical populations and the control seeds showed that populations and 298 have higher nuclear DNA content (C2) than untreated hybrid (C2 &. They prevent megaspore mother cell (MMC) from entering meiosis and bypass mitosis directly or impedes cytokinesis after entrance...

Aberrant expression of CD54 detected by flow cytometry is a characteristic of B-lymphoma cells in bone marrow specimens

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Aberrant expression of CD54 detected by flow cytometry is a characteristic. of B-lymphoma cells in bone marrow specimens. Background: Flow cytometry (FC) is a popular method to detect bone marrow (BM) involvement in patients with B-cell non-Hodgkin lymphoma (B-NHL). The majority of screen panels of FC still rely on finding monoclonal B-cells, e.g., B-cells with immunoglobin (Ig) light-chain restriction, which...

Utility of a new notation to visualize flow cytometry analysis results: First preliminary comparison with immunohistochemistry to detect CD30 expression on T-cell lymphoma cells

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followed by 3, 2, and 1 “>” correspond to level-I, level-II, or level-III. Eight of the 52 cases were immunohistochemically CD30-negative. In the PRIME-F level-I to III group (excluding false- positive cases out of 23 cases) were <. Contrarily, in the level-IV &. The images or other third party material in this article are included in the article’s Creative Commons...

Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer

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lymphatic exudate from patients with breast cancer. Background: Breast cancer is the most common cancer, and the leading cause of cancer-related deaths, among females world-wide. Recent research suggests that extracellular vesicles (EVs) play a major role in the development of breast cancer metastasis. The overall aim was to isolate and characterize EVs from axillary serous fluid, and more specifically to...