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Chủ đề : BMC Genomics


Có 40+ tài liệu thuộc chủ đề "BMC Genomics"

The immune-modulating pregnancyspecific glycoproteins evolve rapidly and their presence correlates with hemochorial placentation in primates

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In humans, 10 PSG genes encode closely related secreted. In recent years, a role in modulation of the maternal immune system possibly to avoid rejection of the semiallogeneic fetus and to facilitate access of trophoblast cells to maternal resources via the blood system has been suggested. Alternatively, they could serve as soluble pathogen decoy receptors like other members of the...

GCA: An R package for genetic connectedness analysis using pedigree and genomic data

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Results: We developed the GCA R package to perform genetic connectedness analysis for pedigree and genomic data. The software implements a large collection of various connectedness statistics as a function of prediction error variance or variance of unit effect estimates. The GCA R package is available at GitHub and the source code is provided as open source.. Conclusions: The GCA...

Genome-wide identification, characterization, and expression analysis of tea plant autophagy-related genes (CsARGs) demonstrates that they play diverse roles during development and under abiotic stress

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In addition, the expression levels of 10 CsARGs were higher in the cold-resistant cultivar ‘ Longjing43 ’ than in the cold-susceptible cultivar. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.. Identification of...

Genome-wide identification and functional prediction of long non-coding RNAs in Sprague-Dawley rats during heat stress

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Background: Heat stress (HS) is a major stress event in the life of an animal, with detrimental upshots in production and health. 0.05) were identified in the liver and adrenal glands of the Control and H120 groups, respectively. Furthermore and 73 target differentially-expressed genes (DEGs) in the liver were predicted to interact with DElncRNAs based on trans. Similarly, 437, 73...

Metagenomic sequencing revealed the potential of banknotes as a repository of microbial genes

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In the genomic age, genomes of microorganisms from various environments have been deciphered. In this work, we attempted to use the easily accessible banknotes to search for novel microbial gene sequences.. Results: We used high-throughput genomic sequencing technology to comprehensively characterize the diversity of microorganisms on the US dollars and Chinese Renminbis (RMBs). In addition to finding a vast diversity...

QTL mapping of root traits in wheat under different phosphorus levels using hydroponic culture

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QTL mapping of root traits in wheat under different phosphorus levels using. Identification of useful loci for root morphological and P uptake related traits at seedling stage is important for wheat breeding. The most stable QTL QRRS.caas-4DS for ratio of root to shoot dry weight (RRS) harbored the stable genetic region with high phenotypic effect, and QTL clusters on 7A...

Transcriptome analysis of the transdifferentiation of canine BMSCs into insulin producing cells

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Transcriptome analysis of the. Background: Bone marrow mesenchymal stem cells are a potential resource for the clinical therapy of certain diseases. Canine, as a companion animal, living in the same space with human, is an ideal new model for human diseases research. Because of the high prevalence of diabetes, alternative transplantation islets resource (i.e. insulin producing cells) for diabetes treatment...

Comparative chloroplast genomes: insights into the evolution of the chloroplast genome of Camellia sinensis and the phylogeny of Camellia

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Comparative chloroplast genomes: insights into the evolution of the chloroplast. Here, we determined the chloroplast genome of the first natural triploid Chinary type tea. Further, the evolutionary mechanism of the chloroplast genome of Camellia sinensis and the relationships of Camellia species based on chloroplast genome were discussed.. Results: Comparative analysis showed the evolutionary dynamics of chloroplast genome of Camellia sinensis...

Transcriptome profiling of Lymnaea stagnalis (Gastropoda) for ecoimmunological research

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Ecoimmunological studies, however, typically use one/few phenotypic immune assays and thus do not consider the complexity of the immune system. Results: Immune defence factors identified in the de novo assembly covered elements broadly described in other gastropods. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a...

Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs

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As Ixodes scapularis nymph tick bites are responsible for most LD cases, this study sought to identify nymph tick saliva proteins associated with B. Results: We identified a combined 747 tick saliva proteins of uninfected and B. burgdorferi infected ticks. burgdorferi infected ticks.. burgdorferi infection modified protein content in tick saliva to promote its survival at the tick feeding site....

Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos

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Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts. To understand the conditions that compromise developmental competence of in vitro embryos, it is crucial to evaluate the transcriptional profile of porcine embryos during pre- implantation stages. In this study, we...

Assessment of heterosis based on parental genetic distance estimated with SSR and SNP markers in upland cotton (Gossypium hirsutum L.)

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Assessment of heterosis based on parental genetic distance estimated with SSR and SNP markers in upland cotton ( Gossypium hirsutum L.). Genetic distance (GD) is one of the valuable criteria for selecting parents in hybrid breeding.. The objectives of this study were to estimate the GD between parents using both simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP)...

Genome-wide characterization of 2- oxoglutarate and Fe(II)-dependent dioxygenase family genes in tomato during growth cycle and their roles in metabolism

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In addition, type I flavone synthase (FNSI) belongs to the 2ODD family and contributes to the biosynthesis of flavones, but this protein has not been characterized in tomato.. The Sl2ODD s in the same clade showed similar intron/exon distributions and conserved motifs. The Sl2ODD s were unevenly distributed across the 12 chromosomes, with different expression patterns among major tissues and...

Blinatumomab-induced T cell activation at single cell transcriptome resolution

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Here, blinatumomab-induced transcriptional changes reflected the functional immune activity of the blinatumomab-activated T cells, including the upregulation of pathways such as the immune system, glycolysis, IFNA signaling, gap junctions, and IFNG signaling. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material....

Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam]

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Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Enzymes in the first class prefer pectic β-(1 → 4)-galactan as the substrate, and enzymes in the other prefer the β-(1 → 3) and (1 → 6)-galactan backbones of arabinogalactan pro- teins [7, 8]. A typical bgal protein contains the GH35 conserved...

Transcriptomics analysis of Toxoplasma gondii-infected mouse macrophages reveals coding and noncoding signatures in the presence and absence of MyD88

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and MyD88. Only a minimal number of transcripts were differentially expressed between MyD88 knockout and wild type cells. gondii genes during infection, no differentially expressed parasite genes were identified when comparing infection in MyD88 knockout and wild type host BMDM.. Dual RNA sequencing of Toxoplasma-infected macrophages identifies host and parasite transcripts We infected wild type and MyD88 knockout (KO) bone...

Genome sequencing, annotation and exploration of the SO2-tolerant nonconventional yeast Saccharomycodes ludwigii

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Genome sequencing, annotation and exploration of the SO 2 -tolerant non- conventional yeast Saccharomycodes ludwigii. ludwigii UTAD17 genome using PacBio as well as the insights gathered from the exploration of the annotation performed over the assembled genome.. ludwigii UTAD17 genome with PacBio resulted in 20 contigs totaling 13 Mb of assembled DNA and corresponding to 95% of the DNA harbored...

Genome-wide miRNA analysis and integrated network for flavonoid biosynthesis in Osmanthus fragrans

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Results: In this study, we used high-throughput sequencing technology to analyze the expression profiles of miRNAs in leaf and flower tissues of O. In the end, flavonoid content was measured in flower and leaf tissues and potential role of miR858 in regulating flavonoid synthesis was illustrated in O. Conclusions: This study not only provided the genome-wide miRNA profiles in the...

An intriguing characteristic of enhancer-promoter interactions

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We also noticed that although a fraction of these clusters of enhancers do overlap with super-enhancers, the majority of the enhancer clusters are different from the known super-enhancers.. Early experimental studies identify enhancers by“enhancer trap”, which has established our rudimentary understand- ing of enhancers in spite of its low-throughput and time- consuming nature [10, 11]. A large number of enhancers...

A comparative analysis of heart microRNAs in vertebrates brings novel insights into the evolution of genetic regulatory networks

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morphophysiological differences in the fully formed heart of these species, such as chamber septation, heart rate frequency, blood pressure, and cardiac output volume. Conclusions: Both the conserved and group-specific miRNAs can be considered modulators orchestrating the core and peripheral genes of heart GRNs of vertebrates, which can be related to the morphophysiological differences and similarities existing in the heart of...